RESUMO
Wohlfahrtiimonas chitiniclastica is an emerging zoonotic bacterium commensally living in larvae of particular flies. It has been associated with human and animal infections but never isolated from food. In the present study, a whole chicken carcass was rinsed in buffered peptone water which was then inoculated into BHI and the growth plated onto selective medium. Species identification was performed by MALDI-TOF MS. Those bacteria identified as W. chitiniclastica were subjected to 16S rRNA sequencing for confirmation and MEGA software was used to obtain their phylogenetic position. The findings of this study raise concerns regarding the abattoir, transport and stock practices of frozen meat carcasses and should be of interest with regard to microbiology, entomology and food production.
Assuntos
Galinhas/microbiologia , Alimentos Congelados/microbiologia , Carne/microbiologia , Xanthomonadaceae/classificação , Xanthomonadaceae/isolamento & purificação , Zoonoses/microbiologia , Animais , Brasil , Doenças Transmissíveis Emergentes/microbiologia , DNA Bacteriano/genética , Dípteros/microbiologia , Manipulação de Alimentos , Microbiologia de Alimentos , Filogenia , RNA Ribossômico 16S/genética , Infecção dos Ferimentos/microbiologia , Xanthomonadaceae/química , Xanthomonadaceae/genéticaRESUMO
We report the first case of fulminant sepsis due to Wohlfahrtiimonas chitiniclastica. This case is also the first one reported in South America. We emphasize the importance of recognizing bacteria that live in the larvae of a parasitic fly as the causative agent of severe infections in homeless patients.
Assuntos
Infecções por Bactérias Gram-Negativas/diagnóstico , Sepse/microbiologia , Xanthomonadaceae/isolamento & purificação , Idoso , Animais , Antibacterianos/farmacologia , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Dípteros/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Pessoas Mal Alojadas , Humanos , Masculino , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , América do Sul , Xanthomonadaceae/classificação , Xanthomonadaceae/genética , Xanthomonadaceae/fisiologiaRESUMO
OBJECTIVE: The aim of this study was to correlate the presence of Enterobacteriaceae, Pseudomonadaceae, Moraxellaceae and Xanthomonadaceae on the posterior dorsum of the human tongue with the presence of tongue coating, gender, age, smoking habit and denture use. MATERIAL AND METHODS: Bacteria were isolated from the posterior tongue dorsum of 100 individuals in MacConkey agar medium and were identified by the API 20E system (Biolab-Mérieux). RESULTS: 43% of the individuals, presented the target microorganisms on the tongue dorsum, with greater prevalence among individuals between 40 and 50 years of age (p = 0.001) and non-smokers (p=0.0485). CONCLUSIONS: A higher prevalence of Enterobacteriaceae and Pseudomonadaceae was observed on the tongue dorsum of the individuals evaluated. There was no correlation between these species and the presence and thickness of tongue coating, gender and presence of dentures.
Assuntos
Enterobacteriaceae/isolamento & purificação , Pseudomonadaceae/isolamento & purificação , Língua/microbiologia , Adulto , Fatores Etários , Contagem de Colônia Microbiana , Prótese Total/microbiologia , Prótese Parcial Fixa/microbiologia , Prótese Parcial Removível/microbiologia , Dentaduras , Enterobacter cloacae/isolamento & purificação , Enterobacteriaceae/classificação , Feminino , Halitose/microbiologia , Humanos , Masculino , Mannheimia haemolytica/isolamento & purificação , Pessoa de Meia-Idade , Moraxellaceae/classificação , Moraxellaceae/isolamento & purificação , Higiene Bucal , Pasteurella pneumotropica/isolamento & purificação , Pseudomonadaceae/classificação , Fumar , Língua/patologia , Xanthomonadaceae/classificação , Xanthomonadaceae/isolamento & purificaçãoRESUMO
OBJECTIVE: The aim of this study was to correlate the presence of Enterobacteriaceae, Pseudomonadaceae, Moraxellaceae and Xanthomonadaceae on the posterior dorsum of the human tongue with the presence of tongue coating, gender, age, smoking habit and denture use. MATERIAL AND METHODS: Bacteria were isolated from the posterior tongue dorsum of 100 individuals in MacConkey agar medium and were identified by the API 20E system (Biolab-Mérieux). RESULTS: 43 percent of the individuals, presented the target microorganisms on the tongue dorsum, with greater prevalence among individuals between 40 and 50 years of age (p = 0.001) and non-smokers (p=0.0485). CONCLUSIONS: A higher prevalence of Enterobacteriaceae and Pseudomonadaceae was observed on the tongue dorsum of the individuals evaluated. There was no correlation between these species and the presence and thickness of tongue coating, gender and presence of dentures.
Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Enterobacteriaceae/isolamento & purificação , Pseudomonadaceae/isolamento & purificação , Língua/microbiologia , Fatores Etários , Contagem de Colônia Microbiana , Dentaduras , Prótese Total/microbiologia , Prótese Parcial Fixa/microbiologia , Prótese Parcial Removível/microbiologia , Enterobacter cloacae/isolamento & purificação , Enterobacteriaceae/classificação , Halitose/microbiologia , Mannheimia haemolytica/isolamento & purificação , Moraxellaceae/classificação , Moraxellaceae/isolamento & purificação , Higiene Bucal , Pasteurella pneumotropica/isolamento & purificação , Pseudomonadaceae/classificação , Fumar , Língua/patologia , Xanthomonadaceae/classificação , Xanthomonadaceae/isolamento & purificaçãoRESUMO
Three mesophilic bacteria (strains AMX 26B(T), UR374_02 and 12-3(T)) isolated respectively from an anaerobic digester, human urine and urban riverside soil were characterized. Cells were Gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum and had a strictly respiratory metabolism with O(2) as the preferential terminal electron acceptor. Phylogenetic analysis based on 16S rRNA gene sequences revealed that all strains clustered within the Xanthomonadaceae branch of the Proteobacteria. Isolates AMX 26B(T) and UR374_02 exhibited 100 % 16S rRNA gene sequence similarity and both were related to strain 12-3(T) (99.6 % similarity). The closest relative of all the isolates was Pseudoxanthomonas broegbernensis DSM 12573(T) (similarity 97.1-97.5 %), and they were equidistantly related to Xanthomonas species (95.4-96.6 %), Stenotrophomonas species (95.3-96.1 %) and Pseudoxanthomonas taiwanensis ATCC BAA-4040(T) (95.3-95.4 %). Chemotaxonomic and biochemical data (branched-chain cellular fatty acid pattern without C(13 : 0) iso 3-OH, ubiquinone with eight isoprenoid units, limited range of substrates used, ability to reduce nitrite but not nitrate with the production of N(2)O) supported their affiliation to the genus Pseudoxanthomonas. The results of DNA-DNA hybridization and/or phenotypic analysis allowed them to be differentiated from the two Pseudoxanthomonas species with validly published names and showed that strain 12-3(T) was genomically and phenotypically distinct from the other two isolates. On the basis of these results, two novel species of the genus Pseudoxanthomonas are proposed: Pseudoxanthomonas mexicana sp. nov., consisting of strains AMX 26B(T) (=ATCC 700993(T)=CIP 106674(T)=JCM 11524(T)) (type strain) and UR374_02 (=DSM 15133), and Pseudoxanthomonas japonensis sp. nov., consisting of strain 12-3(T) (=CCUG 48231(T)=CIP 107388(T)=JCM 11525(T)). The report of these two novel species leads to the emendation of the description of the genus Pseudoxanthomonas and the re-evaluation of the phenotype of P. broegbernensis DSM 12573(T) necessitates the emendation of its description.