RESUMO
INTRODUCTION: The promising non-clinical antileishmanial effects of gentian violet (GV) encouraged us to evaluate the additive effect of GV on cryotherapy. METHODS: For 8 weeks, 59/161 cutaneous leishmaniasis patients/lesions underwent cryotherapy alone (group 1) or cryotherapy accompanied by 1% GV application (group 2). The primary endpoint was clinical response. RESULTS: Ultimately, 54.7% and 45.3% of the significantly cured lesions belonged to groups 1 and 2, respectively, which was not statistically significant. The clinical response was significantly different between the two groups at the end of the fourth week. CONCLUSIONS: Although the clinical response of the two groups was significantly different at the end of the fourth week, application of GV did not increase the efficacy of cryotherapy.
Assuntos
Anti-Infecciosos Locais/administração & dosagem , Crioterapia/métodos , Violeta Genciana/administração & dosagem , Leishmaniose Cutânea/tratamento farmacológico , Adulto , Feminino , Seguimentos , Humanos , Masculino , Projetos Piloto , Método Simples-Cego , Resultado do TratamentoRESUMO
Abstract INTRODUCTION: The promising non-clinical antileishmanial effects of gentian violet (GV) encouraged us to evaluate the additive effect of GV on cryotherapy. METHODS: For 8 weeks, 59/161 cutaneous leishmaniasis patients/lesions underwent cryotherapy alone (group 1) or cryotherapy accompanied by 1% GV application (group 2). The primary endpoint was clinical response. RESULTS: Ultimately, 54.7% and 45.3% of the significantly cured lesions belonged to groups 1 and 2, respectively, which was not statistically significant. The clinical response was significantly different between the two groups at the end of the fourth week. CONCLUSIONS: Although the clinical response of the two groups was significantly different at the end of the fourth week, application of GV did not increase the efficacy of cryotherapy.
Assuntos
Humanos , Masculino , Feminino , Adulto , Leishmaniose Cutânea/tratamento farmacológico , Crioterapia/métodos , Violeta Genciana/administração & dosagem , Anti-Infecciosos Locais/administração & dosagem , Método Simples-Cego , Projetos Piloto , Seguimentos , Resultado do TratamentoRESUMO
La parotiditis crónica conocida también como parotiditis juvenil recurrente, se define como la inflamación recurrente de la glándula parótida, asociada a la aparición de sialectasias o dilataciones no obstructivas de los conductos glandulares. Como tratamiento se emplea habitualmente la sialografía que tiene una tasa de éxito que va de 62 por ciento a 94 por ciento. Se presenta aquí un caso de un niño de 4 años de edad con parotiditis crónica, que persiste con episodios de parotiditis, pese a la realización de dos sialografías con un intervalo de 1 año entre ambas. Se decide inyectar violeta genciana, procedimiento que no tuvo complicaciones, después de la inyección remitió la enfermedad
Chronic parotitis, also known as juvenile recurrent parotits, is defined as the recurrent inflammation of the parotid gland, associated to the appearance of sialectasis or non-obstructive dilation of the glandular ducts. Sialography is regularly employed as a treatment, with success rates ranging between 62 and 94 percent. Here we present the case of a 4 year old child with chronic parotitis, that persisted with parotitis episodes despite having two sialographies performed, with one year interval between them. We decided to inject methyl violet, a procedure that was carried out without any complications; and the disease resolved shortly after the injection
Assuntos
Humanos , Masculino , Pré-Escolar , Parotidite/diagnóstico , Parotidite/terapia , Corantes/administração & dosagem , Doença Crônica , Glândula Parótida , Resultado do Tratamento , Sialografia , Violeta Genciana/administração & dosagemAssuntos
Anti-Infecciosos Locais/administração & dosagem , Cateteres de Demora/efeitos adversos , Violeta Genciana/administração & dosagem , Granuloma de Corpo Estranho/etiologia , Diálise Peritoneal Ambulatorial Contínua/efeitos adversos , Administração Tópica , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/etiologia , Infecções Bacterianas/microbiologia , Granuloma de Corpo Estranho/tratamento farmacológico , HumanosRESUMO
BACKGROUND: Allogeneic blood transfusions are the second most frequent route of Chagas' disease transmission in countries where the disease is endemic. The prevention of transfusion-associated Chagas' disease has been attempted through clinical and serologic screening of blood donors and/or by the addition of trypanomicidal substances such as gentian violet (GV) to stored blood for 24 hours. The present study describes an alternative method of chemoprophylaxis of transfusion-associated Chagas' disease that reduces the sterilization time by using a combination of low-concentration GV, ascorbic acid (AA), and photoradiation with visible light. STUDY DESIGN AND METHODS: To better reproduce the conditions of blood transfusion in developing areas, normal human blood was collected in blood collection bags, infected with different concentrations of Trypanosoma cruzi, and treated with GV, AA, and photoradiation. Mice were then inoculated with the T. cruzi-infected human blood that had been stored at different incubation intervals. Active parasites were sought in mouse blood for parasitologic diagnosis and serologic evaluation (mice inoculation, blood culture, and indirect immunofluorescence). RESULTS: The association of GV (250 micrograms/mL), and photoradiation with visible light (75W) sterilized T. cruzi-infected blood even after treatment for less than 30 minutes and even when chagasic blood was treated with low-concentration GV (62.5 micrograms/mL for 30 min). Moreover, the trypanomicidal activity of GV associated with AA and photoradiation with visible light was found even when blood was infected with a 10-fold parasite concentration. CONCLUSIONS: The proposed alternative prophylactic method is reproducible, easy to perform, and inexpensive, and it may have practical importance in endemic areas where serologic screening of donor blood is not always available. In addition, the reduction of the GV trypanomicidal concentration might further minimize the potential for GV-related side effects.
Assuntos
Ácido Ascórbico , Transfusão de Sangue , Doença de Chagas/prevenção & controle , Violeta Genciana , Luz , Esterilização , Animais , Doença de Chagas/transmissão , Relação Dose-Resposta a Droga , Violeta Genciana/administração & dosagem , Humanos , Camundongos , Fatores de Tempo , Trypanosoma cruzi/efeitos dos fármacos , Trypanosoma cruzi/efeitos da radiaçãoRESUMO
O canal radicular de 160 dentes foi obturado com OZE ou Sealapex. Após a impermeabilizaçäo radicular eles foram imersos em azul de metileno a 2 por cento, fluoresceína a 2 por cento, violeta de genciana a 2 por cento e rhodamina B a 1 por cento, onde permaneceram por 24 ou 168 horas. Foram a seguir, secionados longitudinalmente, procedendo-se as leituras específicas da microinfiltraçäo. As maiores magnitudes de infiltraçäo foram observadas com a fluoresceína e a rhodamina B, seguindo-se o azul de metileno e a violeta de genciana, sendo que o azul de metileno apresentou a menor variedade e a fluorescína a maior. O cimento Sealapex mostrou-se melhor selador que o OZE