RESUMO
BACKGROUND: Mammospheres are breast cancer stem cells (BCSCs) that could be yielded through culturing cells in non-adherent and non-differentiating condition. With regard to therapy resistance of cancer stem cells (CSCs), it is essential to discover efficient approaches targeting CSCs. Viola odorata extract has been considered as a traditional herbal anti-metastatic drug in several cancer cells. Effect of this drug on BCSCs has not been clearly identified. Current study tries to detect and to compare effect of Viola odorata extract on malignant characterization of breast cancer cell lines and BCSCs. MATERIALS AND METHODS: MCF7 and SKBR3 and their derived mammospheres as BCSCs were used and the effect of alcoholic extraction of Viola odorata on apoptosis and malignant characters of MCF7, SKBR3 and their derived BCSCs were analyzed and compared. RESULTS: Viola odorata extract induced cell death in MCF7, SKBR3 and their derived mammospheres through apoptosis without any effects on MCF10A. Also, this extract showed anti-migratory, anti-invasion and anti-colony formation activity in MCF7, SKBR3 and their derived mammospheres which was significantly more in MCF7- and SKBR3-derived mammospheres. Also, this extract decreased size and volume of tumors generated by MCF7, SKBR3 and their derived mammospheres in chicken embryo model. CONCLUSION: Viola odorata extract exerted anti-cancerous activity on both breast cancer cell lines and their derived BCSCs. Anti-cancerous activity of this extract was significantly more in MCF7-, SKBR3-derived mammospheres in comparison with dedicated cell lines. Data suggest that Viola odorata extract mostly targets cancerous cells, not normal cells with exception in high concentration. It acts in a cell-dependent manner.
Assuntos
Neoplasias da Mama/tratamento farmacológico , Células-Tronco Neoplásicas/efeitos dos fármacos , Extratos Vegetais/farmacologia , Viola/química , Animais , Apoptose/efeitos dos fármacos , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Embrião de Galinha , Feminino , Humanos , Células-Tronco Neoplásicas/patologia , Esferoides CelularesRESUMO
Heartsease, also known as wild pansy (Viola tricolor L.), contains considerable amounts of polyphenols: 109.32 ± 1.29 mg of Gallic acid equivalent/g of extract. This study investigates their phytoconstituents and antioxidant capacity and validates a method for the quantification of rutin in the crude extract of the flowers of V. tricolor and in the extract incorporated in gel. Much better antioxidant capacity was found for the extract [inhibition concentration (IC50) of 16.00 ± 0.78 µg/mL] than the standard ascorbic acid (IC50 of 16.57 ± 0.95 µg/mL); these excellent results may be attributable to the amounts of polyphenols, flavonoids and condensed tannins. The high-performance liquid chromatography method for the quantification of rutin in the extract and gel was linear, sensitive, precise, specific, accurate and robust. This validated method can be used to control the quality of the extract and the gel.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Extratos Vegetais/análise , Rutina/análise , Viola/química , Antioxidantes/análise , Antioxidantes/isolamento & purificação , Géis/análise , Extratos Vegetais/isolamento & purificação , Rutina/isolamento & purificaçãoRESUMO
This work deals with the ultrasonic degradation (800 kHz) of crystal violet (CV) under different experimental conditions. The effects of saturating gas (argon, carbon dioxide and air), CV concentration (2.45-1225 µmol L(-1)), pH (3-9) and power (20-80 W) were evaluated. The best performances were obtained at 80 W with argon as a saturating gas. The pH had no significant effect. The influence of several water matrices containing anions (chloride, sulphate and bicarbonate) and cations (Fe(2+)) on the sonolytic degradation of CV was also investigated. Significant differences were not observed with the presence of chloride and sulphate. However, at relatively low pollutant concentration (2.45 µmol L(-1)) bicarbonate showed a particular effect: a high bicarbonate concentration (350 mmol L(-1)) produced a detrimental effect, while a low bicarbonate concentration (3 mmol L(-1)) increased the efficiency of the process. The presence of Fe(2+) (1 mmol L(-1)) also increased the CV (49 µmol L(-1)) degradation by 32% after 180 min. Analyses of intermediates by GC-MS led to the identification of several sonochemical by-products: N,N-dimethylaminobenzene, 4-(N,N-dimethylamino)-4'-(N',N'-dimethylamino)benzophenone, and N,N,N',N'-tetramethyl-4,4'-diaminodiphenylmethane. The presence of these aromatic structures showed that the main ultrasonic CV degradation pathway is linked to the reaction with *OH radicals. At the end of the treatment, these early products were converted into biodegradable organic by-products which could be easily treated in a subsequent biological treatment.