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1.
mSphere ; 6(4): e0059921, 2021 08 25.
Artigo em Inglês | MEDLINE | ID: mdl-34346702

RESUMO

Vibrio parahaemolyticus is a marine Gram-negative bacterium that is a leading cause of seafood-borne gastroenteritis. Pandemic strains of V. parahaemolyticus rely on a specialized protein secretion machinery known as the type III secretion system 2 (T3SS2) to cause disease. The T3SS2 mediates the delivery of effector proteins into the cytosol of infected cells, where they subvert multiple cellular pathways. Here, we identify a new T3SS2 effector protein encoded by VPA1328 (VP_RS21530) in V. parahaemolyticus RIMD2210633. Bioinformatic analysis revealed that VPA1328 is part of a larger family of uncharacterized T3SS effector proteins with homology to the VopG effector protein in Vibrio cholerae AM-19226. These VopG-like proteins are found in many but not all T3SS2 gene clusters and are distributed among diverse Vibrio species, including V. parahaemolyticus, V. cholerae, V. mimicus, and V. diabolicus and also in Shewanella baltica. Structure-based prediction analyses uncovered the presence of a conserved C-terminal kinase domain in VopG orthologs, similar to the serine/threonine kinase domain found in the NleH family of T3SS effector proteins. However, in contrast to NleH effector proteins, in tissue culture-based infections, VopG did not impede host cell death or suppress interleukin 8 (IL-8) secretion, suggesting a yet undefined role for VopG during V. parahaemolyticus infection. Collectively, our work reveals that VopG effector proteins, a new family of likely serine/threonine kinases, is widely distributed in the T3SS2 effector armamentarium among marine bacteria. IMPORTANCE Vibrio parahaemolyticus is the leading bacterial cause of seafood-borne gastroenteritis worldwide. The pathogen relies on a type III secretion system to deliver a variety of effector proteins into the cytosol of infected cells to subvert cellular function. In this study, we identified a novel Vibrio parahaemolyticus effector protein that is similar to the VopG effector of Vibrio cholerae. VopG-like effectors were found in diverse Vibrio species and contain a conserved serine/threonine kinase domain that bears similarity to the kinase domain in the enterohemorrhagic Escherichia coli (EHEC) and Shigella NleH effectors that manipulate host cell survival pathways and host immune responses. Together our findings identify a new family of Vibrio effector proteins and highlight the role of horizontal gene transfer events among marine bacteria in shaping T3SS gene clusters.


Assuntos
Proteínas de Bactérias/genética , Proteínas Serina-Treonina Quinases/genética , Sistemas de Secreção Tipo III/genética , Vibrio parahaemolyticus/enzimologia , Vibrio parahaemolyticus/genética , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Células CACO-2 , Biologia Computacional , Regulação Bacteriana da Expressão Gênica , Humanos , Interleucina-8/imunologia , Família Multigênica , Transporte Proteico , Serina/metabolismo , Sistemas de Secreção Tipo III/metabolismo , Vibrio parahaemolyticus/metabolismo , Vibrio parahaemolyticus/patogenicidade
2.
Rev. argent. microbiol ; Rev. argent. microbiol;45(4): 267-70, dic. 2013.
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-1171794

RESUMO

The enzymatic characterization of vibrios has been used as a virulence indicator of sanitary interest. The objective of this study was to determine the enzymatic profile of Vibrio parahaemolyticus strains (n=70) isolated from Crassostrea rhizophorae oysters. The strains were examined for the presence of gelatinase (GEL), caseinase (CAS), elastase (ELAS), phospholipase (PHOS), lipase (LIP), amilase (AML) and DNase. All enzymes, except elastase, were detected in more than 60


of the strains. The most recurrent enzymatic profiles were AML + DNase + PHOS + GEL + LIP (n=16; 22.9


) and AML + CAS + DNase + PHOS + GEL + LIP (n=21; 30


). Considering the fact that exoenzyme production by vibrios is closely related to virulence, one must be aware of the bacteriological risk posed to human health by the consumption of raw or undercooked oysters.


Assuntos
Crassostrea/microbiologia , Vibrio parahaemolyticus/classificação , Vibrio parahaemolyticus/enzimologia , Animais , Vibrio parahaemolyticus/isolamento & purificação
3.
Rev Argent Microbiol ; 45(4): 267-70, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24401782

RESUMO

The enzymatic characterization of vibrios has been used as a virulence indicator of sanitary interest. The objective of this study was to determine the enzymatic profile of Vibrio parahaemolyticus strains (n=70) isolated from Crassostrea rhizophorae oysters. The strains were examined for the presence of gelatinase (GEL), caseinase (CAS), elastase (ELAS), phospholipase (PHOS), lipase (LIP), amilase (AML) and DNase. All enzymes, except elastase, were detected in more than 60% of the strains. The most recurrent enzymatic profiles were AML + DNase + PHOS + GEL + LIP (n=16; 22.9%) and AML + CAS + DNase + PHOS + GEL + LIP (n=21; 30%). Considering the fact that exoenzyme production by vibrios is closely related to virulence, one must be aware of the bacteriological risk posed to human health by the consumption of raw or undercooked oysters.


Assuntos
Crassostrea/microbiologia , Vibrio parahaemolyticus/classificação , Vibrio parahaemolyticus/enzimologia , Animais , Vibrio parahaemolyticus/isolamento & purificação
4.
Rev. argent. microbiol ; 45(4): 267-70, 2013 Oct-Dec.
Artigo em Espanhol | BINACIS | ID: bin-132750

RESUMO

The enzymatic characterization of vibrios has been used as a virulence indicator of sanitary interest. The objective of this study was to determine the enzymatic profile of Vibrio parahaemolyticus strains (n=70) isolated from Crassostrea rhizophorae oysters. The strains were examined for the presence of gelatinase (GEL), caseinase (CAS), elastase (ELAS), phospholipase (PHOS), lipase (LIP), amilase (AML) and DNase. All enzymes, except elastase, were detected in more than 60


of the strains. The most recurrent enzymatic profiles were AML + DNase + PHOS + GEL + LIP (n=16; 22.9


) and AML + CAS + DNase + PHOS + GEL + LIP (n=21; 30


). Considering the fact that exoenzyme production by vibrios is closely related to virulence, one must be aware of the bacteriological risk posed to human health by the consumption of raw or undercooked oysters.


Assuntos
Crassostrea/microbiologia , Vibrio parahaemolyticus/classificação , Vibrio parahaemolyticus/enzimologia , Animais , Vibrio parahaemolyticus/isolamento & purificação
5.
Rev Inst Med Trop Sao Paulo ; 33(4): 263-5, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-1844947

RESUMO

Of 21 human fecal strains of Vibrio parahaemolyticus, isolated on the Northeast Coast of Brazil, eight (38%) were urease positive. Most of these strains, in contrast to the urease-negative ones, did not produce the hemolysin responsible for the Kanagawa phenomenon.


Assuntos
Urease/metabolismo , Vibrio parahaemolyticus/isolamento & purificação , Adulto , Brasil , Fezes/parasitologia , Humanos , Vibrio parahaemolyticus/enzimologia
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