RESUMO
We investigated neovasculization effects of embolus-carried human vascular endothelial cell growth factor 165 (VEGF165)-encoded adenovirus (Ad) vector in the hindlimbs of rats with thromboangiitis obliterans (TAO). Rats were equally divided into blank control (I), TAO model (II), embolus (III), Ad-VEGF165 intravascular treatment (IV), Ad-VEGF165 intramuscular treatment (V), and embolus-carried Ad-VEGF165 (VI) groups. After interventional treatment, the neovasculization effect of the test gene was observed using immunohistochemistry. At 1 week after administration, compared with group II, groups V and VI had significantly increased microvessel densities, but no significant difference was observed between groups V and VI. At 2 weeks, groups V and VI exhibited significantly increased microvessel densities. At 1 week after administration, compared with group II, both groups V and VI showed a significant difference in the ratio between the α-smooth muscle actin count and the muscle fiber count, whereas no significant difference was observed between them. At 2 weeks, groups V and VI also exhibited significant differences in these ratios compared with the other groups. We conclude that Ad-VEGF165 promotes neovasculization in ischemic limbs. Embolus-carried Ad- VEGF165 had the most pronounced effect.
Assuntos
Terapia Genética , Neovascularização Patológica/genética , Tromboangiite Obliterante/genética , Fator A de Crescimento do Endotélio Vascular/genética , Adenoviridae/genética , Animais , Embolia/genética , Embolia/terapia , Células Endoteliais/patologia , Extremidades/patologia , Técnicas de Transferência de Genes , Humanos , Isquemia/genética , Isquemia/terapia , Ratos , Tromboangiite Obliterante/patologia , Tromboangiite Obliterante/terapia , Fator A de Crescimento do Endotélio Vascular/biossínteseRESUMO
Thromboangiitis obliterans (TAO) is an inflammatory, nonocclusive, and nonatherosclerotic vascular disease. It commonly affects arteries, veins, and surrounding neural elements and is directly related to smoking. Although distal vessels of lower and upper extremities are the most commonly involved, other vessels such as intestinal arteries can be rarely affected. The authors describe a 41-year-old white male smoker who presented with abdominal pain for 3 months and developed an acute bowel ischemia. He underwent urgent surgery, and segmental enterectomy was performed. Histopathologic findings were suggestive of TAO, showing typical involvement of small-sized veins and arteries with intact internal elastic lamina, preserved media, a local nonspecific inflammatory reaction, with new and older arterial and venous thromboses associated. Although mesenteric arteries are seldom injured by TAO, this diagnosis must be considered when the usual causes of intestinal ischemia are ruled out. In this case, even without any other clinical symptoms of TAO, this rare diagnosis could be made.
Assuntos
Intestinos/irrigação sanguínea , Isquemia/etiologia , Tromboangiite Obliterante/complicações , Adulto , Humanos , Isquemia/patologia , Isquemia/terapia , Masculino , Tromboangiite Obliterante/patologia , Tromboangiite Obliterante/terapiaRESUMO
The case of a 26-year-old male chronic smoker is reported, who had thrombotic incidences on three occasions in both upper and lower limbs over a 10-month period. Laboratory examinations, including hematologic studies and creatinine, cholesterol, and glucose levels, were normal. However, IgG and IgM anticardiolipin antibodies were detected. Duplex ultrasonography confirmed deep venous thrombosis in both lower limbs (two occurrences) and computer tomography depicted an inferior cava vein thrombosis. The patient fulfilled all of Shionoya's criteria for Buerger's disease, thus suggesting an association between anticardiolipin antibodies and this disease.
Assuntos
Anticorpos Anticardiolipina/sangue , Síndrome Antifosfolipídica/complicações , Tromboangiite Obliterante/diagnóstico , Adulto , Anticoagulantes/uso terapêutico , Heparina/uso terapêutico , Humanos , Masculino , Prognóstico , Doença de Raynaud/etiologia , Fumar/efeitos adversos , Abandono do Hábito de Fumar , Tromboangiite Obliterante/tratamento farmacológico , Tromboangiite Obliterante/etiologia , Tromboangiite Obliterante/terapia , Tromboflebite/etiologia , Varfarina/uso terapêuticoRESUMO
Se analiza el papel preponderante que juega en la medicina actual la valoración del lecho microcirculatorio en varias patologías. Se hace un repaso de los métodos de diágnostico disponibles. Se presenta una clasificación clínica de Acrosíndromes.
Assuntos
Humanos , Gasometria/métodos , Diagnóstico Clínico , Hemorreologia , Microcirculação/patologia , Doença de Raynaud/diagnóstico , Doença de Raynaud/terapia , Vasculite/classificação , Vasculite/diagnóstico , Vasculite/terapia , Angiopatias Diabéticas/fisiopatologia , Cianose/classificação , Pé Diabético , Eritema Endurado , Eritema Nodoso , Dermatopatias Vasculares , Tromboangiite Obliterante/terapiaRESUMO
Se analiza el papel preponderante que juega en la medicina actual la valoración del lecho microcirculatorio en varias patologías. Se hace un repaso de los métodos de diágnostico disponibles. Se presenta una clasificación clínica de Acrosíndromes. (AU)
Assuntos
Humanos , Microcirculação/patologia , Hemorreologia/métodos , Diagnóstico Clínico , Gasometria/métodos , Vasculite/classificação , Vasculite/diagnóstico , Vasculite/terapia , Doença de Raynaud/diagnóstico , Doença de Raynaud/terapia , Dermatopatias Vasculares , Cianose/classificação , Eritema Endurado , Eritema Nodoso , Tromboangiite Obliterante/terapia , Angiopatias Diabéticas/fisiopatologia , Pé DiabéticoRESUMO
Objetivos. Conocer las características clínicas de la presentación de la enfermedad de Buerger en el Hospital San Juan de Dios de Santa Fe de Bogotá
Assuntos
Humanos , Tromboangiite Obliterante , Tromboangiite Obliterante/diagnóstico , Tromboangiite Obliterante/etiologia , Tromboangiite Obliterante/fisiopatologia , Tromboangiite Obliterante/terapiaRESUMO
La tromboangeítis obliterante o enfermedad de Buerger ha sido reconocida como entidad patológica desde hace más de 80 años. Afecta primordialmente las arterias de miembros inferiores. Dentro de los criterios utilizados para su diagnóstico se incluyen los cambios arteriográficos en pacientes con enfermedad de Buerger. Informamos nuestra experiencia en estudios arteriográficos en pacientes con enfermedad de Buerger en los últimos cinco años. Llamamos la atención sobre la alta frecuencia de lesiones proximales a la arteria poplítea (57 por ciento). Los signos arteriográficos más frecuentes encontrados fueron tortuosidad de los vasos (57 por ciento), adelgazamiento progresivo del lumen (50 por ciento), alternancia de la lesión (50 por ciento), adelgazamiento abrupto (50 por ciento), siendo menos frecuentes los signos clásicamente descritos para la enfermedad como lo son: colaterales en tirabuzón (29 por ciento), signo de Martorrell (21 por ciento), corrugado de la pared del vaso (14 por ciento) e imagen en raíz de árbol (7 por ciento). Todas las arteriografías mostraron múltiples hallazgos de compromiso vascular, entre 3 y 7 de los signos descritos para la enfermedad