RESUMO
Hemolytic activity of 7 isolates and 2 clones of Trichomonas vaginalis and 1 isolate and 2 clones of Tritrichomonas suis was determined using incubation with erythrocytes. T. vaginalis hemolyzed all human blood groups, and no correlation between pathogenicity and hemolytic activity was observed and no hemolysin released by the parasite could be detected. No hemolytic activity was observed with strains and clones of T. suis against erythrocytes from all blood groups and with swine erythrocytes.
Assuntos
Hemólise , Trichomonas vaginalis/patogenicidade , Tritrichomonas/patogenicidade , Animais , Antígenos de Grupos Sanguíneos , Células Clonais , Concanavalina A/farmacologia , Feminino , Humanos , Infecções por Protozoários/parasitologia , Infecções Protozoárias em Animais , Especificidade da Espécie , Suínos/parasitologia , Doenças dos Suínos/parasitologia , Vaginite por Trichomonas/parasitologia , Trichomonas vaginalis/efeitos dos fármacos , Tritrichomonas/efeitos dos fármacos , VirulênciaRESUMO
The general structure of Tritrichomonas foetus incubated in the presence of the peptide antibiotic polymyxin B, which interacts specifically with anionic phospholipids, was analyzed using transmission electron microscopy of thin sections, and freeze-fracture replicas. Polymyxin B induced morphological changes in the plasma membrane of the parasites with the formation of membrane blebs with a diameter varying from 65 nm to 1.5 micron. Freeze-fracture images of the membrane lining the blebs showed that their inner membrane half is smooth. However, membrane particles, with a density similar to that observed on the E face of the plasma membrane, were seen on the outer half of this membrane.
Assuntos
Polimixina B/farmacologia , Polimixinas/farmacologia , Tritrichomonas/ultraestrutura , Animais , Membrana Celular/efeitos dos fármacos , Membrana Celular/ultraestrutura , Técnica de Fratura por Congelamento , Histocitoquímica , Microscopia Eletrônica , Tritrichomonas/efeitos dos fármacosRESUMO
1. The effect of metronidazole, a drug used in the treatment of trichomoniasis, on the surface chage of both Trichomonas vaginalis and Tritrichomonas foetus was analyzed by cell electrophoresis. 2. Incubation of the parasites under anaerobic conditions for 2 or 120 min in the presence of 2.0 microng/ml metronidazole, a concentration which inhibits cell growth by about 50%, led to a marked decrease in the net surface charge of the parasites. 3. The metronidazole analogue, 1-hydroxyethyl-2-methyl-1-4-inhibited protozoan growth but had no effect on met surface charge. 4. These observations indicate that, in addition to its effect on intracellular structure, cuch as hydrogenosomes, metronidazole alters the surface of trichomonads