RESUMO
Anthelmintic resistance is a worldwide concern in small ruminant industry and new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Mentha piperita, Cymbopogon martinii and Cymbopogon schoenanthus essential oils were evaluated against developmental stages of trichostrongylids from sheep naturally infected (95% Haemonchus contortus and 5% Trichostrogylus spp.) through the egg hatch assay (EHA), larval development assay (LDA), larval feeding inhibition assay (LFIA), and the larval exsheathment assay (LEA). The major constituent of the essential oils, quantified by gas chromatography for M. piperita oil was menthol (42.5%), while for C. martinii and C. schoenanthus the main component was geraniol (81.4% and 62.5%, respectively). In all in vitro tests C. schoenanthus essential oil had the best activity against ovine trichostrongylids followed by C. martini, while M. piperita presented the least activity. Cymbopogon schoenanthus essential oil had LC(50) value of 0.045 mg/ml in EHA, 0.063 mg/ml in LDA, 0.009 mg/ml in LFIA, and 24.66 mg/ml in LEA. The anthelmintic activity of essential oils followed the same pattern in all in vitro tests, suggesting C. schoenanthus essential oil could be an interesting candidate for nematode control, although in vivo studies are necessary to validate the anthelmintic properties of this oil.
Assuntos
Anti-Helmínticos/farmacologia , Cymbopogon/química , Haemonchus/efeitos dos fármacos , Mentha piperita/química , Óleos Voláteis/farmacologia , Trichostrongylus/efeitos dos fármacos , Monoterpenos Acíclicos , Animais , Anti-Helmínticos/química , Cromatografia Gasosa-Espectrometria de Massas , Haemonchus/crescimento & desenvolvimento , Haemonchus/fisiologia , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Larva/fisiologia , Óleos Voláteis/química , Testes de Sensibilidade Parasitária , Óleos de Plantas/química , Óleos de Plantas/farmacologia , Ovinos , Terpenos/química , Terpenos/farmacologia , Trichostrongylus/crescimento & desenvolvimento , Trichostrongylus/fisiologiaRESUMO
The anthelmintic (AH) effect of Acacia pennatula, Leucaena leucocephala, Lisyloma latisiliquum and Piscidia piscipula was evaluated in the infective larvae (L(3)) of Trichostrongylus colubriformis. Different concentrations of lyophilized extracts were tested using the larval migration inhibition (LMI) test. An inhibitor of tannins (the polyvinyl polypyrrolidone [PVPP]) was used to verify whether these compounds were responsible for the AH effects. Then, the effect of extracts on larval exsheathment was examined by observing the exsheathment process at 10-min intervals for 70 min. The LMI test showed a dose-dependant AH effect for A. pennatula, L. leucocephala and L. latisiliquum (P<0.01), but not for P. piscipula. The restoration of L(3) migration to values similar to those of controls after the addition of PVPP, indicates that tannins are involved in AH effects. Trichostrongylus colubriformis exsheathment was partially or totally blocked by the four plants extracts. Tropical tanniniferous plants evaluated in the current study may have potential as AH for the control of T. colubriformis if in vivo investigations indicate useful effects.
Assuntos
Anti-Helmínticos/farmacologia , Extratos Vegetais/farmacologia , Trichostrongylus/efeitos dos fármacos , Trichostrongylus/fisiologia , Animais , Anti-Helmínticos/química , Relação Dose-Resposta a Droga , Larva/efeitos dos fármacos , Larva/fisiologia , Extratos Vegetais/química , Folhas de Planta/química , Fatores de Tempo , Clima TropicalRESUMO
The objective of this study was to determine the main sources of variation in herbage densities of infective third stage larvae of goat strongyles during the marked dry season of 1994 in Guadeloupe (FWI). Herbage samples were collected for L3 density (LD) determination by an accurate method, 4 times at 4-week intervals in 58 paddocks of 21 farms spread out in five regions of the archipel of Guadeloupe. At the same time, FEC of each grazing animal and fecal culture for parasite genus determination according to sex and age were carried out. Stocking rate, dry matter content of soil, and daily climatic data were also recorded. An index of egg development in larvae (IEDL) was calculated as the ratio of LD to the eggs deposed during the 4th, 3rd, and 2nd weeks before sampling. Medians of LD in herbage were 3397, 1853, 1410, and 324 L3/kg DM for all parasites, Haemonchus, Trichostrongylus, and Oesophagostomum, respectively. Date of sampling, region, and irrigation practice in the northern windward region were the main sources of variation in LD and in frequency of each parasite. LD decreased as the dryness lasted, but it remained important (500 L3/kg DM) despite the drought. LD in windward regions were higher than in other regions. The region, the farm, and the paddock were the main sources of variation of IEDL. LD of each parasites were inversely correlated to global radiation recorded 1 to 3 weeks before herbage sampling, but no relation was found with rainfall data. Trichostrongylus frequency in L3 population increased as the dryness lasted. A dryness axis was extracted from environmental variables (climatic data, dry matter of soil, duration of dryness) by a multiple factorial procedure. LD and Haemonchus frequency in L3 population were inversely correlated to dryness axis (p < 0.01). In contrast, Trichostrongylus frequency was positively correlated to the dryness component.