RESUMO
El presente trabajo tuvo como finalidad demostrar la toxicidad oral a 45 días del extracto etanólico de Cenchrus echinatus L. "cadillo" en ratas albinas. El diseño es un estudio pre-clinico, desarrollado en la Escuela de Farmacia y Bioqímica, Facultad de Medicina Humana. Universidad San Pedro, chimbote, Perú. Para lo cual se utilizó ratas albinas y extracto etanólico de C. equinatus. La muestra vegetal se recolectó en San José, (Santiago de Cao, Ascope, La Libertad). Se trabajo con dos grupos normales de ratas albinas macho y hembras que recibieron SSF 4mL/kg, y 2 grupos experimentales machos y hembras que recibieron el extracto a dosis de 100 mg/kg, los tratamientos se administraron diariamente por vía oral. Las principales medidas de la toxicidad fueron el aumento de peso corporal, alteracione físicas y conductuales, pruebas hematológicas, homogeneidad de peso de los órganos blandos y fórmula leucocitaria. Se observó que no existe variación significativa entre los grupos que recibieron extracto y controles. Se concluye que en condiciones experimentales, el extracto de C. equinatus no es tóxico cuando se administra en ratas albinas, durante 45 días por vía oral.
The present work was to demonstrate oral toxicity 45 days of ethano extract of Cenchrus echinatus L. "cocklebur" in albino rats. The design is a pre-clinical study, which was developed at the School of Pharmacy and Biochemistry, Faculty of Human Medicine. University of San Pedro, Chimbote, Perú. Which was used for albino rats and ethanol extract of C. equinatus. The plant sample was collected in San José(Santiago de Cao Ascope, La Libertad). For this study considering two normal groups of albino rats (males and females) receiving 4 mL/kg SSF, and 2 groups received extract (males and females) at doses of 100 mg/kg, treatments were administered daily by mouth. The main measure of toxicity included body weight gain, physical and behavioral changes, blood tests, homogeneity of soft organ weights and blood differential. It was observed that there is significant variation between the groups receiving extract and controls. It can be concluded that under experimental conditions, C. equinatus extract is not toxic when administered in albino rats for 45 days orally.
Assuntos
Extratos Vegetais/toxicidade , Plantas Medicinais , Toxicidade , Tribulus/toxicidadeRESUMO
PURPOSE: Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate solution, using phytotherapy is being sought. The present study attempted to evaluate the antilithiatic properties of Tribulus terrestris commonly called as "gokhru" which is often used in ayurveda to treat various urinary diseases including urolithiasis. MATERIALS AND METHODS: The activity of Tribulus terrestris was investigated on nucleation and the growth of the calcium oxalate (CaOx) crystals as well as on oxalate induced cell injury of NRK 52E renal epithelial cells. RESULTS: Tribulus terrestris extract exhibited a concentration dependent inhibition of nucleation and the growth of CaOx crystals. When NRK-52E cells were injured by exposure to oxalate for 72 h, Tribulus terrestris extract prevented the injury in a dose-dependent manner. On treatment with the different concentrations of the plant, the cell viability increased and lactate dehydrogenase release decreased in a concentration dependent manner. CONCLUSION: The current data suggests that Tribulus terrestris extract not only has a potential to inhibit nucleation and the growth of the CaOx crystals but also has a cytoprotective role. Our results indicate that it could be a potential candidate for phytotherapy against urolithiasis.
Assuntos
Oxalato de Cálcio/química , Células Epiteliais/efeitos dos fármacos , Túbulos Renais/efeitos dos fármacos , Extratos Vegetais/farmacologia , Tribulus/química , Urolitíase , Animais , Cristalização , Modelos Animais de Doenças , Células Epiteliais/patologia , Cálculos Renais/induzido quimicamente , Túbulos Renais/citologia , Túbulos Renais/patologia , Fitoterapia , Extratos Vegetais/uso terapêutico , Extratos Vegetais/toxicidade , Ratos , Tribulus/toxicidade , Urolitíase/prevenção & controleRESUMO
PURPOSE: Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate solution, using phytotherapy is being sought. The present study attempted to evaluate the antilithiatic properties of Tribulus terrestris commonly called as gokhru which is often used in ayurveda to treat various urinary diseases including urolithiasis. MATERIALS AND METHODS: The activity of Tribulus terrestris was investigated on nucleation and the growth of the calcium oxalate (CaOx) crystals as well as on oxalate induced cell injury of NRK 52E renal epithelial cells. RESULTS: Tribulus terrestris extract exhibited a concentration dependent inhibition of nucleation and the growth of CaOx crystals. When NRK-52E cells were injured by exposure to oxalate for 72 h, Tribulus terrestris extract prevented the injury in a dose-dependent manner. On treatment with the different concentrations of the plant, the cell viability increased and lactate dehydrogenase release decreased in a concentration dependent manner. CONCLUSION: The current data suggests that Tribulus terrestris extract not only has a potential to inhibit nucleation and the growth of the CaOx crystals but also has a cytoprotective role. Our results indicate that it could be a potential candidate for phytotherapy against urolithiasis.