Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Intervalo de ano de publicação
1.
Acta Cir Bras ; 33(10): 896-903, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30484499

RESUMO

PURPOSE: To investigate the apoptotic mechanisms in rabbits with blast-induced acute lung injury (ALI). METHODS: A total of 40 rabbits were randomly divided into a blank control group (A, n=10) and an experimental group (EXP, n=30). Explosion-induced chest-ALI models were prepared and sampled at different time points (4, 12, and 24h after modeling, T1-T3) to test the lung dry weight/wet weight ratio (W/D) and arterial oxygen pressure (PaO2), apoptosis of lung tissue by the TUNEL assay, and Caspase-3, Bax, and Bcl-2 levels by immunohistochemical analysis. Furthermore, lung tissue was sampled to observe pathological morphology by microscopy. RESULTS: Under a light microscope, Group EXP exhibited obvious edema in the pulmonary interstitial substance and alveoli, a large number of red blood cells, inflammatory cells, and serous exudation in the alveolar cavity, as well as thickening of the pulmonary interstitial fluid. Compared to Group A, the W/D ratio was significantly increased in Group EXP (P<0.01), while PaO2 was significantly reduced (P<0.01). The apoptosis index was significantly increased (P<0.01), and caspase-3 and Bax/Bcl-2 levels were increased (P<0.01). CONCLUSION: Apoptosis plays an important role in the occurrence and development of acute lung injury in rabbits by participating in lung injury and promoting the progression of ALI.


Assuntos
Lesão Pulmonar Aguda/fisiopatologia , Apoptose/fisiologia , Traumatismos por Explosões/fisiopatologia , Lesão Pulmonar Aguda/sangue , Lesão Pulmonar Aguda/patologia , Animais , Traumatismos por Explosões/sangue , Traumatismos por Explosões/patologia , Caspase 3/sangue , Modelos Animais de Doenças , Feminino , Masculino , Proteínas Proto-Oncogênicas c-bcl-2/sangue , Alvéolos Pulmonares/patologia , Coelhos , Distribuição Aleatória , Proteína X Associada a bcl-2/sangue
2.
Acta cir. bras ; Acta cir. bras;33(10): 896-903, Oct. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-973463

RESUMO

Abstract Purpose: To investigate the apoptotic mechanisms in rabbits with blast-induced acute lung injury (ALI). Methods: A total of 40 rabbits were randomly divided into a blank control group (A, n=10) and an experimental group (EXP, n=30). Explosion-induced chest-ALI models were prepared and sampled at different time points (4, 12, and 24h after modeling, T1-T3) to test the lung dry weight/wet weight ratio (W/D) and arterial oxygen pressure (PaO2), apoptosis of lung tissue by the TUNEL assay, and Caspase-3, Bax, and Bcl-2 levels by immunohistochemical analysis. Furthermore, lung tissue was sampled to observe pathological morphology by microscopy. Results: Under a light microscope, Group EXP exhibited obvious edema in the pulmonary interstitial substance and alveoli, a large number of red blood cells, inflammatory cells, and serous exudation in the alveolar cavity, as well as thickening of the pulmonary interstitial fluid. Compared to Group A, the W/D ratio was significantly increased in Group EXP (P<0.01), while PaO2 was significantly reduced (P<0.01). The apoptosis index was significantly increased (P<0.01), and caspase-3 and Bax/Bcl-2 levels were increased (P<0.01). Conclusion: Apoptosis plays an important role in the occurrence and development of acute lung injury in rabbits by participating in lung injury and promoting the progression of ALI.


Assuntos
Animais , Masculino , Feminino , Coelhos , Traumatismos por Explosões/fisiopatologia , Apoptose/fisiologia , Lesão Pulmonar Aguda/fisiopatologia , Alvéolos Pulmonares/patologia , Traumatismos por Explosões/patologia , Traumatismos por Explosões/sangue , Distribuição Aleatória , Proteínas Proto-Oncogênicas c-bcl-2/sangue , Modelos Animais de Doenças , Proteína X Associada a bcl-2/sangue , Caspase 3/sangue , Lesão Pulmonar Aguda/patologia , Lesão Pulmonar Aguda/sangue
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA