RESUMO
PURPOSE: To investigate if low level laser therapy (LLLT) can decrease spinal cord injuries after temporary induced spinal cord ischemia-reperfusion in rats because of its anti-inflammatory effects. METHODS: Forty eight rats were randomized into two study groups of 24 rats each. In group I, ischemic-reperfusion (I-R) injury was induced without any treatment. Group II, was irradiated four times about 20 minutes for the following three days. The lesion site directly was irradiated transcutaneously to the spinal direction with 810 nm diode laser with output power of 150 mW. Functional recovery, immunohistochemical and histopathological changes were assessed. RESULTS: The average functional recovery scores of group II were significantly higher than that the score of group I (2.86 ± 0.68, vs 1.38 ± 0.09; p<0.05). Histopathologic evaluations in group II were showed a mild changes in compare with group I, that suggested this group survived from I-R consequences. Moreover, as seen from TUNEL results, LLLT also protected neurons from I-R-induced apoptosis in rats. CONCLUSION: Low level laser therapy was be able to minimize the damage to the rat spinal cord of reperfusion-induced injury.
Assuntos
Terapia com Luz de Baixa Intensidade/métodos , Traumatismo por Reperfusão/radioterapia , Traumatismos da Medula Espinal/radioterapia , Isquemia do Cordão Espinal/radioterapia , Medula Espinal/irrigação sanguínea , Animais , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Lasers Semicondutores/uso terapêutico , Masculino , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Traumatismos da Medula Espinal/reabilitação , Isquemia do Cordão Espinal/reabilitação , Fatores de Tempo , Resultado do TratamentoRESUMO
To investigate if low level laser therapy (LLLT) can decrease spinal cord injuries after temporary induced spinal cord ischemia-reperfusion in rats because of its anti-inflammatory effects. Forty eight rats were randomized into two study groups of 24 rats each. In group I, ischemic-reperfusion (I-R) injury was induced without any treatment. Group II, was irradiated four times about 20 minutes for the following three days. The lesion site directly was irradiated transcutaneously to the spinal direction with 810 nm diode laser with output power of 150 mW. Functional recovery, immunohistochemical and histopathological changes were assessed. The average functional recovery scores of group II were significantly higher than that the score of group I (2.86 ± 0.68, vs 1.38 ± 0.09; p<0.05). Histopathologic evaluations in group II were showed a mild changes in compare with group I, that suggested this group survived from I-R consequences. Moreover, as seen from TUNEL results, LLLT also protected neurons from I-R-induced apoptosis in rats. Low level laser therapy was be able to minimize the damage to the rat spinal cord of reperfusion-induced injury.(AU)
Assuntos
Animais , Masculino , Terapia com Luz de Baixa Intensidade/métodos , Traumatismo por Reperfusão/radioterapia , Traumatismos da Medula Espinal/radioterapia , Isquemia do Cordão Espinal/radioterapia , Medula Espinal/irrigação sanguínea , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Lasers Semicondutores/uso terapêutico , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Traumatismos da Medula Espinal/reabilitação , Isquemia do Cordão Espinal/reabilitação , Fatores de Tempo , Resultado do TratamentoRESUMO
PURPOSE:To investigate if low level laser therapy (LLLT) can decrease spinal cord injuries after temporary induced spinal cord ischemia-reperfusion in rats because of its anti-inflammatory effects.METHODS: Forty eight rats were randomized into two study groups of 24 rats each. In group I, ischemic-reperfusion (I-R) injury was induced without any treatment. Group II, was irradiated four times about 20 minutes for the following three days. The lesion site directly was irradiated transcutaneously to the spinal direction with 810 nm diode laser with output power of 150 mW. Functional recovery, immunohistochemical and histopathological changes were assessed.RESULTS:The average functional recovery scores of group II were significantly higher than that the score of group I (2.86 ± 0.68, vs 1.38 ± 0.09; p<0.05). Histopathologic evaluations in group II were showed a mild changes in compare with group I, that suggested this group survived from I-R consequences. Moreover, as seen from TUNEL results, LLLT also protected neurons from I-R-induced apoptosis in rats.CONCLUSION:Low level laser therapy was be able to minimize the damage to the rat spinal cord of reperfusion-induced injury.
Assuntos
Animais , Masculino , Terapia com Luz de Baixa Intensidade/métodos , Traumatismo por Reperfusão/radioterapia , Traumatismos da Medula Espinal/radioterapia , Isquemia do Cordão Espinal/radioterapia , Medula Espinal/irrigação sanguínea , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Lasers Semicondutores/uso terapêutico , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Traumatismos da Medula Espinal/reabilitação , Isquemia do Cordão Espinal/reabilitação , Fatores de Tempo , Resultado do TratamentoRESUMO
It remains unknown if the oxidative stress can be regulated by low-level laser therapy (LLLT) in lung inflammation induced by intestinal reperfusion (i-I/R). A study was developed in which rats were irradiated (660 nm, 30 mW, 5.4 J) on the skin over the bronchus and euthanized 2 h after the initial of intestinal reperfusion. Lung edema and bronchoalveolar lavage fluid neutrophils were measured by the Evans blue extravasation and myeloperoxidase (MPO) activity respectively. Lung histology was used for analyzing the injury score. Reactive oxygen species (ROS) was measured by fluorescence. Both expression intercellular adhesion molecule 1 (ICAM-1) and peroxisome proliferator-activated receptor-y (PPARy) were measured by RT-PCR. The lung immunohistochemical localization of ICAM-1 was visualized as a brown stain. Both lung HSP70 and glutathione protein were evaluated by ELISA. LLLT reduced neatly the edema, neutrophils influx, MPO activity and ICAM-1 mRNA expression. LLLT also reduced the ROS formation and oppositely increased GSH concentration in lung from i-I/R groups. Both HSP70 and PPARy expression also were elevated after laser irradiation. Results indicate that laser effect in attenuating the acute lung inflammation is driven to restore the balance between the pro- and antioxidants mediators rising of PPARy expression and consequently the HSP70 production.