RESUMO
En la unidad de Fertilidad y Esterilidad de la Clínica Avila de Caracas (UNIFERTES), entre 1988 y 1990 se practicaron treinta transferencias intratubarias de gametos (GIFT) a veinte y seis pacientes con historias de esterilidad de dos o más años de evolución, las cuales fueron estudiadas desde el punto de vista de fertilidad sin la laparoscopia diagnóstica previa. Las pacientes fueron sometidas a estimulación ovárica con citrato de clomifeno más MG más H.C.G y monitorizada su evolución con ultrasonografía transvaginal y valores de estradiol sérico. Transferimos de 3 a 5 oocitos metafase II en una o ambas trompas, con un número de espermatozoides mótiles entre 100.000 y 150.000 por ml. Se obtuvieron nueve embarazos (30%), siendo éxito seis de ellos (20%), terminando 3 de en parto vaginal y 3 en cesárea. El peso promedio de los niños al nacer fue de 3112 g. No hubo malformaciones congénitas
Assuntos
Humanos , Feminino , Transferência Intrafalopiana de Gameta/instrumentação , Transferência Intrafalopiana de Gameta/métodos , Citratos/uso terapêutico , Fertilização/genética , Fertilização/fisiologiaRESUMO
A procedure utilizing transvaginal aspiration of stimulated gametes followed by transcervical, ultrasound-guided catheterization of the tubal ostia was performed as a modification of the standardized gamete intrafallopian transfer (GIFT) technique. Among 14 patients with 16 cycles there were four normal, intrauterine pregnancies and one ectopic pregnancy. In two patients the beta-human gonadotropin level rose significantly and then started to fall; the patients aborted spontaneously. The procedure can be performed with a higher degree of patient acceptance than can traditional GIFT, and the success rate in this small series was promising.
Assuntos
Transferência Intrafalopiana de Gameta/métodos , Adulto , Cateterismo , Comportamento do Consumidor , Feminino , Humanos , Ultrassonografia , VaginaRESUMO
A new technique for vaginal intratubal insemination (VITI) and transvaginal gamete intra-Fallopian transfer (TV-GIFT) using endosonography has been developed. A total of 47 infertile couples were treated using a K-JITS 1000-1100 catheter (William Cook, Australia; Jansen Anderson Intratubal Transfer Set) introduced by Jansen and Anderson in Sydney, Australia, and endosonographic control with a Kretz Combison 310 echograph and a 5/7.5 MHz vaginal sectorial transducer. In 40 couples, the VITI technique was used, and in the other seven couples, TV-GIFT was used. Fifteen pregnancies (37.5%) were obtained in 63 VITI cycles (23.8% per treatment cycle) and three with TV-GIFT (42% per treatment cycle). A review is also presented of the migration--sedimentation technique used for management of male factor in an artificial insemination programme.
Assuntos
Transferência Intrafalopiana de Gameta/métodos , Inseminação Artificial Heteróloga/métodos , Inseminação Artificial Homóloga/métodos , Inseminação Artificial/métodos , Ultrassom/métodos , Adulto , Feminino , Humanos , MasculinoRESUMO
Techniques of "In Vitro" fertilization (IFV) and gamete intrafallopian transfer (GIFT) are proving to be successful in the treatment of infertility. We report here in detail the laboratory procedures of sperm processing for these two techniques also in the present work we pointing out the media quality control methods and the incubation time for the maintenance of spermatozoa fertilizing ability.