RESUMO
O trauma é frequentemente relatado na rotina clínica de pequenos animais, podendo gerar fraturas de componentes ósseos e lesões de tecidos moles adjacentes. No presente trabalho, objetivou-se relatar o caso de um canino, macho, sem raça definida, de sete meses de idade, vítima de trauma craniomaxilofacial decorrente de mordedura, diagnosticado com fratura e deslocamento de osso zigomático, além de importante laceração de pele. O tratamento estabelecido baseou-se no debridamento da ferida, estabilização cirúrgica de arco zigomático com fios de Nylon e na sutura dos ferimentos de pele causados. Em um segundo tempo cirúrgico, foi realizada enucleação e recobrimento do defeito na órbita com malha cirúrgica de polipropileno, além de remoção de tecido exuberante e confecção de retalho subdérmico de avanço facial lateral para recobrimento do defeito cutâneo. A complicação evidenciada na primeira intervenção cirúrgica foi a deiscência das suturas de pele, enquanto no segundo tempo cirúrgico, não houve sinais de complicações. No pós-operatório, o paciente apresentou evolução clínica satisfatória, recebendo alta médica 21 dias após o segundo procedimento cirúrgico realizado. Tendo em vista a adequada evolução clínica, bem como os reduzidos efeitos colaterais, sugere-se que a conduta clínica e técnicas cirúrgicas adotadas para tratamento do paciente em questão foram efetivas.
Trauma is frequently reported in the small animal clinics, and can lead to fractures of cranial bone components and injuries to the adjacent soft tissues. In the present study, the objective was to report the case of a seven-month-old male mongrel dog, that had a craniomaxillofacial trauma resulting from a bite, characterized by zygomatic bone fracture and displacement, in addition to a major skin laceration. The stablished treatment was based on wound debridement, surgical stabilization of the zygomatic arch with nylon threads and in the suturing of skin wounds. In a second surgical procedure, enucleation was performed and a surgical polypropylene mesh was applied to cover the orbital defect, exuberant tissue was removed and a subdermal advancement flap was used to cover the skin defect. The complication observed in the first surgical intervention was dehiscence of the skin sutures, while in the second surgical procedure, there were no signs of complications. Postoperatively, the patient had a satisfactory clinical recovery, being discharged 21 days after the second surgical procedure. Considering the adequate clinical evolution and the reduced complications, it issuggestedthat the clinical conduct and surgical techniques adopted for the treatment of the patient in question were effective.
Assuntos
Animais , Cães , Polipropilenos , Retalhos Cirúrgicos/veterinária , Telas Cirúrgicas/veterinária , Ferimentos e Lesões/veterinária , Zigoma/cirurgia , Procedimentos de Cirurgia Plástica/veterinária , Cães/cirurgia , Face/cirurgiaRESUMO
OBJECTIVES: To test the feasibility of a new device for gasless laparoscopy in providing working space for diaphragmatic hernia repair in an ex vivo canine model as a pre-clinical study. STUDY DESIGN: Technical feasibility study. ANIMAL: Eight beagles and two greyhound cadavers (not client-owned). METHODS: The new device was used for abdominal traction in gasless laparoscopic reconstruction of diaphragmatic hernias produced in dog cadavers. It consists of three main parts (vertical and horizontal rods, a three-piece structure, and a 3D-printed unit that incorporates slots for haemostatic forceps). Composite hernias (two incisions of about 4 cm) were closed by an intra-corporeal suture [suture group (GS), n = 5] or by a central suture and a polypropylene mesh [mesh group (GM), n = 5]. Surgical steps were T1 (primary port access up to third port placement), T2 (defect development), and T3 (diaphragmatic reconstruction). Total surgical time (TT) was also recorded. RESULTS: The defect was successfully developed and reconstructed in all cadavers. To close the defect, 7.0 ± 0.7 crossed mattress sutures were required in the GS, and 15.2 ± 1.9 hernia staples and one intra-corporal suture were used in the GM. T3 was longer (p = 0.0076) in GS (50.00 ± 16.46 min) than in GM (23.24 ± 5.25 min). TT was 87.22 ± 19.23 min in GS and 66.45 ± 6.38 min in GM (p = 0.0547). CONCLUSIONS: Gasless laparoscopic diaphragmatic hernia repair using the developed device is feasible in the canine cadaver model. Both suture and mesh graft techniques for experimental diaphragmatic herniorrhaphy can be performed using this new device in this pre-clinical model. CLINICAL SIGNIFICANCE: This new device for gasless laparoscopy allows diaphragmatic herniorrhaphy by intra-corporeal suture or mesh implantation in ex vivo canine model. The device demonstrates potential for future use in clinical cases.
Assuntos
Doenças do Cão , Hérnia Diafragmática , Laparoscopia , Animais , Cadáver , Doenças do Cão/cirurgia , Cães , Hérnia Diafragmática/cirurgia , Hérnia Diafragmática/veterinária , Herniorrafia/métodos , Herniorrafia/veterinária , Laparoscopia/veterinária , Telas Cirúrgicas/veterináriaRESUMO
Purpose: To evaluate fibrosis formation and number of macrophages in capsules formed around textured implants without and with mesh coverage. Methods: Fibrosis was analyzed through transforming growth factor-beta 1 (TGF-ß1) immunomarker expression and the number of macrophages through CD68 percentage of cells in magnified field. Sixty female Wistar rats were distributed into two groups of 30 rats (unmeshed and meshed). Each group was then subdivided into two subgroups for postoperative evaluation after 30 and 90 days. The p value was adjusted by Bonferroni lower than 0.012. Results: No difference was observed in fibrosis between meshed and unmeshed groups (30 days p = 0.436; 90 days p = 0.079) and from 30 to 90 days in the unmeshed group (p = 0.426). The meshed group showed higher fibrosis on the 90th day (p = 0.001). The number of macrophages was similar between groups without and with mesh coverage (30 days p = 0.218; 90 days p = 0.044), and similar between subgroups 30 and 90 days (unmeshed p = 0.085; meshed p = 0.059). Conclusions: In the meshed group, fibrosis formation was higher at 90 days and the mesh-covered implants produced capsules similar to microtextured ones when analyzing macrophages. Due to these characteristics, mesh coating did not seem to significantly affect the local fibrosis formation.
Assuntos
Animais , Feminino , Ratos , Telas Cirúrgicas/veterinária , Fibrose/veterinária , Antígenos CD/análise , Implantes de Mama/veterinária , Implante Mamário/instrumentação , Fator de Crescimento Transformador beta1/análise , Ratos Wistar/cirurgiaRESUMO
Purpose To evaluate the effect of a PP mesh on duct deferens morphology, testicular size and testosterone levels. Methods Forty adult male rats were distributed into groups: 1) no surgery; 2) inguinotomy; 3) mesh placed on the duct deferens; and 4) mesh placed on the spermatic funiculus. After 90 postoperative days, the inguinal region was resected, and blood samples were collected for the measurement of serum testosterone (pg/dl). The ducts deferens were sectioned in three axial sections according to the relationship with the mesh cranial, medial and caudal. The wall thickness and duct deferens lumen area were measured. Results The morphology of the duct deferens was preserved in all groups. The mesh placement did not alter this morphology in any of the analyzed segments. Surgery, with or without mesh placement, did not alter the morphology, wall thickness or lumen area (p>0.05). In all operated groups, serum testosterone levels were similar (p>0.05) but there was a decrease in testicle size (p 0.05). Conclusion Surgery, with or without mesh placement, did not alter the morphology of the duct deferens and, although this treatment resulted in testicular size reduction, it did not affect serum testosterone levels.(AU)
Assuntos
Animais , Masculino , Ratos , Polipropilenos/efeitos adversos , Ducto Deferente/crescimento & desenvolvimento , Testículo/crescimento & desenvolvimento , Telas Cirúrgicas/efeitos adversos , Telas Cirúrgicas/veterinária , Testosterona/análise , Hormônios Esteroides Gonadais/análiseRESUMO
Background: Malignant peripheral nerve sheath tumors are neurogenic neoplasms that originate from cells that surroundthe axons of peripheral nerves. Surgery is the treatment of choice for peripheral nerve sheath tumors. They have a betterprognosis when the lesion is in the extremity of a limb and the surgeon leaves wide peripheral margins after resection.However, this procedure makes local treatment a challenge due to difficult wound healing in this region. This report describes a successful case involving the use of a meshed skin graft immediately after resection of a neurofibrosarcoma inthe distal region of the radius bone of a dog.Case: A 6-year-old Boxer bitch weighing 40 kg was admitted with a history of a round, firm, non-ulcerated skin noduleattached to the lateral side of the distal region of the right radius bone, which had been present for about 40 days. Fineneedle aspiration cytology of the lesion showed the presence of mesenchymal cells, suggesting a sarcoma. Thus, the decision was made for an incisional biopsy to confirm the diagnosis and for the preparation of a subdermal pattern tubular flapfor subsequent rotation and transposition to close the wound that would be formed after the complete removal of the lesion.After the 7th postoperative day, the diagnosis of low-grade neurofibrosarcoma was confirmed and due to the presence ofnecrotic onset in the middle portion of the tubular flap, further surgical intervention was scheduled for the resection of thetube flap, en bloc removal of the neoplastic lesion with peripheral margins of 2 cm, and wound closure with a free skingraft. A mesh skin graft was made with a portion of the right flank skin. The mesh graft was carefully implanted on therecipient bed using simple interrupted sutures with a 3-0 non-absorbable monofilament suture material. A dressing madewith water-based sterile lubricating solution and...(AU)
Assuntos
Animais , Feminino , Cães , Transplante de Pele/veterinária , Telas Cirúrgicas/veterinária , Rádio (Anatomia)/cirurgia , Neurofibrossarcoma/cirurgia , Neurofibrossarcoma/veterinária , Neoplasias de Bainha Neural/veterináriaRESUMO
Background: Malignant peripheral nerve sheath tumors are neurogenic neoplasms that originate from cells that surroundthe axons of peripheral nerves. Surgery is the treatment of choice for peripheral nerve sheath tumors. They have a betterprognosis when the lesion is in the extremity of a limb and the surgeon leaves wide peripheral margins after resection.However, this procedure makes local treatment a challenge due to difficult wound healing in this region. This report describes a successful case involving the use of a meshed skin graft immediately after resection of a neurofibrosarcoma inthe distal region of the radius bone of a dog.Case: A 6-year-old Boxer bitch weighing 40 kg was admitted with a history of a round, firm, non-ulcerated skin noduleattached to the lateral side of the distal region of the right radius bone, which had been present for about 40 days. Fineneedle aspiration cytology of the lesion showed the presence of mesenchymal cells, suggesting a sarcoma. Thus, the decision was made for an incisional biopsy to confirm the diagnosis and for the preparation of a subdermal pattern tubular flapfor subsequent rotation and transposition to close the wound that would be formed after the complete removal of the lesion.After the 7th postoperative day, the diagnosis of low-grade neurofibrosarcoma was confirmed and due to the presence ofnecrotic onset in the middle portion of the tubular flap, further surgical intervention was scheduled for the resection of thetube flap, en bloc removal of the neoplastic lesion with peripheral margins of 2 cm, and wound closure with a free skingraft. A mesh skin graft was made with a portion of the right flank skin. The mesh graft was carefully implanted on therecipient bed using simple interrupted sutures with a 3-0 non-absorbable monofilament suture material. A dressing madewith water-based sterile lubricating solution and...
Assuntos
Feminino , Animais , Cães , Rádio (Anatomia)/cirurgia , Telas Cirúrgicas/veterinária , Transplante de Pele/veterinária , Neoplasias de Bainha Neural/veterinária , Neurofibrossarcoma/cirurgia , Neurofibrossarcoma/veterináriaRESUMO
The aim of this study was to characterize the tissue reactions triggered by the polypropylene mesh coated with chitosan and polyethylene glycol film, and if it's able to prevent the formation of peritoneal adhesions. Defects in the abdominal wall of rats were induced and polypropylene meshes coated with chitosan/polyethylene glycol (CPEG group, n= 12) and uncoated (PP control group, n= 12) were implanted. On the fourth and forty-fifth postoperative day the formation of adhesion and the tissue reaction to the biomaterial was evaluated through histological and histochemical analysis. The area (P= 0.01) and severity (P= 0.002) of the adhesion was significatively less in the CPEG group. On the fourth day the foreign body reaction was less intense in CPEG group (P= 0.018) and the production of collagen fibers was more intense in this group (P= 0.041). The tissue reactions caused by the biomaterials were similar on the 45th day, with the exception of the high organization of collagen fibers in the CPEG group. The CPEG meshes did not fully prevent the formation of adhesions, but minimized the severity of the process. The foreign body reaction promoted by polypropylene meshes coated with CPEG is less intense than that triggered by uncoated polypropylene meshes.(AU)
O objetivo deste estudo foi caracterizar as reações tissulares desencadeadas pela tela de polipropileno revestida com o filme de quitosana e polietilenoglicol e verificar se ela é capaz de prevenir a formação de aderências peritoneais. Um defeito na parede abdominal dos ratos foi realizado, e as telas de polipropileno revestidas com quitosana/polietilenoglicol (grupo CPEG, n= 12) e sem revestimento (grupo controle PP, n= 12) foram implantadas. No quarto e no 45º dia pós-operatório, avaliou-se a formação de aderências e a reação tecidual ao biomaterial por análise histológica e histoquímica. A área (P= 0,01) e a severidade (P= 0,002) da aderência peritoneal foram significativamente menores no grupo CPEG no 45º dia. No quarto dia, observou-se que a reação do corpo estranho foi menor no grupo CPEG (P= 0,018), e a produção de fibras de colágeno mais intensa (P= 0,041). As reações tissulares causadas pelos biomateriais implantados foram semelhantes no 45º dia, com exceção da melhor organização das fibras colágenas no grupo CPEG. As telas CPEG não impediram completamente a formação de aderências, porém minimizaram a gravidade do processo. A reação de corpo estranho promovida por telas de polipropileno revestidas com CPEG é menos intensa do que a desencadeada por telas de polipropileno não revestidas.(AU)
Assuntos
Animais , Ratos , Polietilenoglicóis , Polipropilenos , Telas Cirúrgicas/veterinária , Reação a Corpo Estranho/veterinária , Parede Abdominal/cirurgia , Quitosana , Aderências Teciduais/veterináriaRESUMO
The aim of this study was to characterize the tissue reactions triggered by the polypropylene mesh coated with chitosan and polyethylene glycol film, and if it's able to prevent the formation of peritoneal adhesions. Defects in the abdominal wall of rats were induced and polypropylene meshes coated with chitosan/polyethylene glycol (CPEG group, n= 12) and uncoated (PP control group, n= 12) were implanted. On the fourth and forty-fifth postoperative day the formation of adhesion and the tissue reaction to the biomaterial was evaluated through histological and histochemical analysis. The area (P= 0.01) and severity (P= 0.002) of the adhesion was significatively less in the CPEG group. On the fourth day the foreign body reaction was less intense in CPEG group (P= 0.018) and the production of collagen fibers was more intense in this group (P= 0.041). The tissue reactions caused by the biomaterials were similar on the 45th day, with the exception of the high organization of collagen fibers in the CPEG group. The CPEG meshes did not fully prevent the formation of adhesions, but minimized the severity of the process. The foreign body reaction promoted by polypropylene meshes coated with CPEG is less intense than that triggered by uncoated polypropylene meshes.(AU)
O objetivo deste estudo foi caracterizar as reações tissulares desencadeadas pela tela de polipropileno revestida com o filme de quitosana e polietilenoglicol e verificar se ela é capaz de prevenir a formação de aderências peritoneais. Um defeito na parede abdominal dos ratos foi realizado, e as telas de polipropileno revestidas com quitosana/polietilenoglicol (grupo CPEG, n= 12) e sem revestimento (grupo controle PP, n= 12) foram implantadas. No quarto e no 45º dia pós-operatório, avaliou-se a formação de aderências e a reação tecidual ao biomaterial por análise histológica e histoquímica. A área (P= 0,01) e a severidade (P= 0,002) da aderência peritoneal foram significativamente menores no grupo CPEG no 45º dia. No quarto dia, observou-se que a reação do corpo estranho foi menor no grupo CPEG (P= 0,018), e a produção de fibras de colágeno mais intensa (P= 0,041). As reações tissulares causadas pelos biomateriais implantados foram semelhantes no 45º dia, com exceção da melhor organização das fibras colágenas no grupo CPEG. As telas CPEG não impediram completamente a formação de aderências, porém minimizaram a gravidade do processo. A reação de corpo estranho promovida por telas de polipropileno revestidas com CPEG é menos intensa do que a desencadeada por telas de polipropileno não revestidas.(AU)
Assuntos
Animais , Ratos , Polietilenoglicóis , Polipropilenos , Telas Cirúrgicas/veterinária , Reação a Corpo Estranho/veterinária , Parede Abdominal/cirurgia , Quitosana , Aderências Teciduais/veterináriaRESUMO
The incidence of accidents with cats and its consequences on the health of these animals have been reported frequently in the literature. The abdominal hernia is an example of a condition developed as a result of trauma. Abdominal hernia is a defect of the inner wall of the abdomen that can allow part of the abdominal contents to protrude. In this case report, a pregnant cat has developed an abdominal hernia after disappearing for a week. The animal was clinically well, but the abdominal volume was considerable. Surgical intervention was necessary as soon as possible, because it was an irreducible hernia. A significant amount of devitalized muscle tissue and fragile tissue were observed. Because of the abdominal necrosis, there was no possibility to perform conventional suturing technique in the abdominal wall to correct the hernia. It was necessary to apply a polypropylene mesh implant, which is anchored by means of simple interrupted stitches. Besides hernioplasty, a lumpectomy and ovariohysterectomy were performed due to the presence of a suppurative inflammation of the mammary glands and fetal maceration in the uterus. The review of the patient, after 10 days, revealed no signs of infection of the surgical wound and the implant remained stable. The patient continued feeding and drinking water normally. The goal of this study is to describe the surgical technique of hernia repair using polypropylene implant in a cat that has lost approximately 50% of the abdominal muscle tissue caused by an abdominal trauma.(AU)
Assuntos
Animais , Feminino , Gravidez , Gatos , Hérnia Abdominal/cirurgia , Hérnia Abdominal/veterinária , Polipropilenos/análise , Telas Cirúrgicas/veterinária , Próteses e Implantes/veterinária , Herniorrafia/veterináriaRESUMO
The incidence of accidents with cats and its consequences on the health of these animals have been reported frequently in the literature. The abdominal hernia is an example of a condition developed as a result of trauma. Abdominal hernia is a defect of the inner wall of the abdomen that can allow part of the abdominal contents to protrude. In this case report, a pregnant cat has developed an abdominal hernia after disappearing for a week. The animal was clinically well, but the abdominal volume was considerable. Surgical intervention was necessary as soon as possible, because it was an irreducible hernia. A significant amount of devitalized muscle tissue and fragile tissue were observed. Because of the abdominal necrosis, there was no possibility to perform conventional suturing technique in the abdominal wall to correct the hernia. It was necessary to apply a polypropylene mesh implant, which is anchored by means of simple interrupted stitches. Besides hernioplasty, a lumpectomy and ovariohysterectomy were performed due to the presence of a suppurative inflammation of the mammary glands and fetal maceration in the uterus. The review of the patient, after 10 days, revealed no signs of infection of the surgical wound and the implant remained stable. The patient continued feeding and drinking water normally. The goal of this study is to describe the surgical technique of hernia repair using polypropylene implant in a cat that has lost approximately 50% of the abdominal muscle tissue caused by an abdominal trauma.
Assuntos
Feminino , Animais , Gravidez , Gatos , Hérnia Abdominal/cirurgia , Hérnia Abdominal/veterinária , Polipropilenos/análise , Telas Cirúrgicas/veterinária , Herniorrafia/veterinária , Próteses e Implantes/veterináriaRESUMO
The efficiency of the mesh graft used in lacerating wound was evaluated when comparedto another wound treated by second intention. The debridement of the wound wasperformed, the necrotic tissue was removed for subsequent granulation tissue formationand graft application. The transplanted skin was collected from the thoracic region with thesame measures of the defect to be corrected. The preparation of the graft consisted of theremoval of adipose tissue, and later, with the use of the scalpel blade, several rectilinearcuts were made, leaving it with mesh pattern. The graft was fixed to the wound with theuse of a separate single suture. After the surgical procedure, a bandage with hydrogel wasapplied to the limb of the animal, where the dressing change occurred at intervals of fourdays. We concluded that the wound treatment in the MTE using the graft was efficient withtotal wound healing when compared to the non-grafted MTD treatment.Palavras-chaves: cirurgia reconstrutiva; plástica; cão.
Assuntos
Animais , Cães , Lacerações/terapia , Lacerações/veterinária , Pelve/lesões , Telas Cirúrgicas/veterinária , TransplantesRESUMO
The efficiency of the mesh graft used in lacerating wound was evaluated when comparedto another wound treated by second intention. The debridement of the wound wasperformed, the necrotic tissue was removed for subsequent granulation tissue formationand graft application. The transplanted skin was collected from the thoracic region with thesame measures of the defect to be corrected. The preparation of the graft consisted of theremoval of adipose tissue, and later, with the use of the scalpel blade, several rectilinearcuts were made, leaving it with mesh pattern. The graft was fixed to the wound with theuse of a separate single suture. After the surgical procedure, a bandage with hydrogel wasapplied to the limb of the animal, where the dressing change occurred at intervals of fourdays. We concluded that the wound treatment in the MTE using the graft was efficient withtotal wound healing when compared to the non-grafted MTD treatment.Palavras-chaves: cirurgia reconstrutiva; plástica; cão.(AU)
Assuntos
Animais , Cães , Lacerações/terapia , Lacerações/veterinária , Pelve/lesões , Telas Cirúrgicas/veterinária , TransplantesRESUMO
PURPOSE:To investigate whether there is a difference between Marlex(r) and Dynamesh PP-light Marlex(r) meshes, in the abdominal wall defect correction, on rats in contaminated surgical site.METHODS:Twenty-eight Wistar rats were divided into two groups of 14, and four subgroups of seven animals. All subgroups underwent similar surgical procedure. One group received the mesh Marlex(r) and the other Dynamesh PP-light(r) for correction of the defect. Before implanting, the meshes went through a contamination process, on which was used standard solution containing 10 UFC of Escherichia coli. Fragments of the animal's abdominal wall received macroscopic, microscopic and microbiological analysis.RESULTS:There was no statistical significance in the analysis of macroscopic variables. Accentuated inflammatory process was shown in all subgroups. The foreign body type reaction was mild in all subgroups, except Dynamesh(r)-14, which was moderate with no statistical significance. The microbiological analysis of the meshes was also similar between the subgroups.CONCLUSION:There was no difference between the meshes of Marlex(r) and Dynamesh PP-light(r) in the ventral abdominal wall defect correction on rats in contaminated surgical site.(AU)
Assuntos
Animais , Ratos , Parede Abdominal/cirurgia , Telas Cirúrgicas/microbiologia , Telas Cirúrgicas/veterinária , Escherichia coli , Contaminação de Equipamentos , Hérnia Incisional/veterinária , Ratos WistarRESUMO
PURPOSE:To develop an experimental model for incisional hernias and to compare morphological and functional aspects of hernia repairs by suture, polypropylene mesh and collagen mesh.METHODS:A defect measuring 7cm x 2cm was created in the anterior abdominal of 28 New Zealand male rabbits, divided into four groups (n = 7): (1) control, (2) suture of the anterior sheath of the rectus abdominal muscle, (3) setting of polypropylene mesh, and (4) setting of collagen mesh. On the 90th postoperative day, the animals were examined to verify the presence of incisional hernia. Samples of abdominal wall and scar were collected for histological study.RESULTS:Incisional hernia was identified in 85.7% of the control group, 57.1% of the suture group, 42.9% of the collagen mesh group, and none in the polypropylene mesh group (p = 0.015). Mesh exposure could be identified in 71.4% of the animals in group 3 and in no animal in group 4 (p = 0.021). The polypropylene mesh is effective in the treatment of abdominal wall defects, causing an intense inflammatory reaction.CONCLUSION:The collagen mesh is biocompatible, producing a minimal inflammatory reaction, but fails in the treatment of abdominal wall defects.(AU)
Assuntos
Animais , Masculino , Coelhos , Hérnia Abdominal/cirurgia , Hérnia Abdominal/terapia , Hérnia Abdominal/veterinária , Suturas/veterinária , Polipropilenos , Colágeno , Telas Cirúrgicas/veterinária , Próteses e Implantes/veterináriaRESUMO
PURPOSE:To compare fibroplasia and the resistance of the abdominal wall when polypropylene meshes and polypropylene/poliglecaprone are used.METHODS:Seventy-seven male Wistar rats were divided into three groups: Control Group (for resistance); Group E (polypropylene mesh); and Group U (polypropylene/poliglecaprone mesh). The animals in Groups E and U had a standard muscular and aponeurotic defect, with integral peritoneum, and correction with the mesh. Measurements were taken 4, 7, 14, 28 and 56 days after surgery. The resistance, and collagen density were studied.RESULTS:Resistance on the 56th day was similar in both meshes. The gain in resistance described an ascending curve for the polypropylene mesh and was irregular in the case of the polypropylene/poliglecaprone. Fibroplasia showed a gain in type I and type III collagen in both groups (p<0.001). Collagen III stabilized in the 14th day and collagen I continued to ascend.CONCLUSIONS:The gain in resistance of the polypropylene mesh is regular and ascending, whereas the polypropylene/poliglecaprone is not regular. The final resistance of both meshes is similar; the collagen density increases over time, and show the same inflammatory potential.(AU)
Assuntos
Animais , Ratos , Telas Cirúrgicas/veterinária , Colágeno , Hérnia Ventral/veterinária , Cicatrização , Ratos Wistar , PolipropilenosRESUMO
PURPOSE:To evaluate the tissue integration of a double-sided mesh after fixation in diaphragm and to study the diaphragmatic mobility by ultrasound.METHODS:Twenty male Wistar rats were used. The animals were assigned into two equal groups according to the day of euthanasia. The animals were anesthetized and a 1.5 x 1.5 cm of double-layer mesh was inserted between the diaphragm and the liver. For the evaluation of the diaphragm mobility a sonographic method was used. Measurements on specific breathing parameters were taking place. Pathological evaluation took place after the animal's euthanasia.RESULTS:Extra-hepatic granuloma was not differentiated overtime, (χ2=0.04, p>0.05). Neither fibrosis was significantly differentiated, (χ2=0.04, p>0.05). Intra-hepatic granuloma was significantly differentiated overtime, (χ2=10.21, p<0.05). Concerning Te parameter, means were significantly differentiated over time, F (3, 30) = 5.12, (p<0.01). Ttot parameter, it was differentiated over time, F (3, 8)=4.79, (p<0.05). IR parameter was also longitudinally differentiated, F (3, 30)=3.73, (p<0.05).CONCLUSION:The measurements suggest a transient malfunction of diaphragmatic mobility despite the fact that inflammatory reaction, fibrosis and extra-hepatic granuloma were not significantly differentiated with the passage of time.(AU)
Assuntos
Animais , Masculino , Ratos , Diafragma/fisiologia , Telas Cirúrgicas/veterinária , Fibrose/veterinária , Corpos Estranhos/veterinária , Ratos WistarRESUMO
Background: Soft tissue sarcomas are a group of invasive malignant tumors formed by neoplastic mesenchymal cells. In most cases, the treatment require surgical resection. When sarcoma characteristics disqualify conventional tumor excision, polypropylene mesh can be used for abdominal or chest wall reconstruction. This paper aims to describe the clinical, computed tomography features, histopathlogical and immunohistochemical aspects of a chest wall fibrosarcoma, as well as to describe the tumor excision technique combined with resection of multiple ribs, diaphragm advancement and reconstruction of thoracic and abdominal wall with a synthetic polypropylene mesh. Case: An 11-year-old male Boxer was presented with a progressive growth tumor in the left paralumbar area. The invasive tumor measuring 15 cm in diameter, was firm epidermodermal coverage and was adherent to the subcutaneous tissue, having a smooth and non-ulcerative skin surface. Ultrasound of the mass consisted of a heterogeneous structure comprising paralumbar region, invading abdomen and left thoracic wall. Thoracic radiography showed no signs of nodular interstitial pulmonary pattern compatible with metastasis. The dog was submitted to a CT examination of thoracolumbar region, which demonstrated the presence of the circumscribed mass, measuring approximately 17 cm in diameter in the left paralumbar region with...(AU)
Assuntos
Animais , Cães , Polipropilenos , Parede Torácica , Parede Abdominal , Regeneração , Telas Cirúrgicas/veterinária , Fibrossarcoma/cirurgiaRESUMO
Background: Soft tissue sarcomas are a group of invasive malignant tumors formed by neoplastic mesenchymal cells. In most cases, the treatment require surgical resection. When sarcoma characteristics disqualify conventional tumor excision, polypropylene mesh can be used for abdominal or chest wall reconstruction. This paper aims to describe the clinical, computed tomography features, histopathlogical and immunohistochemical aspects of a chest wall fibrosarcoma, as well as to describe the tumor excision technique combined with resection of multiple ribs, diaphragm advancement and reconstruction of thoracic and abdominal wall with a synthetic polypropylene mesh. Case: An 11-year-old male Boxer was presented with a progressive growth tumor in the left paralumbar area. The invasive tumor measuring 15 cm in diameter, was firm epidermodermal coverage and was adherent to the subcutaneous tissue, having a smooth and non-ulcerative skin surface. Ultrasound of the mass consisted of a heterogeneous structure comprising paralumbar region, invading abdomen and left thoracic wall. Thoracic radiography showed no signs of nodular interstitial pulmonary pattern compatible with metastasis. The dog was submitted to a CT examination of thoracolumbar region, which demonstrated the presence of the circumscribed mass, measuring approximately 17 cm in diameter in the left paralumbar region with...
Assuntos
Animais , Cães , Parede Abdominal , Parede Torácica , Polipropilenos , Regeneração , Telas Cirúrgicas/veterinária , Fibrossarcoma/cirurgiaRESUMO
Electrospun materials have been widely explored for biomedical applications because of their advantageous characteristics, i.e., tridimensional nanofibrous structure with high surface-to-volume ratio, high porosity, and pore interconnectivity. Furthermore, considering the similarities between the nanofiber networks and the extracellular matrix (ECM), as well as the accepted role of changes in ECM for hernia repair, electrospun polymer fiber assemblies have emerged as potential materials for incisional hernia repair. In this work, we describe the application of electrospun non-absorbable mats based on poly(ethylene terephthalate) (PET) in the repair of abdominal defects, comparing the performance of these meshes with that of a commercial polypropylene mesh and a multifilament PET mesh. PET and PET/chitosan electrospun meshes revealed good performance during incisional hernia surgery, post-operative period, and no evidence of intestinal adhesion was found. The electrospun meshes were flexible with high suture retention, showing tensile strengths of 3 MPa and breaking strains of 8-33%. Nevertheless, a significant foreign body reaction (FBR) was observed in animals treated with the nanofibrous materials. Animals implanted with PET and PET/chitosan electrospun meshes (fiber diameter of 0.71 ± 0.28 µm and 3.01 ± 0.72 µm, respectively) showed, respectively, foreign body granuloma formation, averaging 4.2-fold and 7.4-fold greater than the control commercial mesh group (Marlex). Many foreign body giant cells (FBGC) involving nanofiber pieces were also found in the PET and PET/chitosan groups (11.9 and 19.3 times more FBGC than control, respectively). In contrast, no important FBR was observed for PET microfibers (fiber diameter = 18.9 ± 0.21 µm). Therefore, we suggest that the reduced dimension and the high surface-to-volume ratio of the electrospun fibers caused the FBR reaction, pointing out the need for further studies to elucidate the mechanisms underlying interactions between cells/tissues and nanofibrous materials in order to gain a better understanding of the implantation risks associated with nanostructured biomaterials.
Assuntos
Materiais Biocompatíveis/farmacologia , Quitosana/farmacologia , Reação a Corpo Estranho/induzido quimicamente , Granuloma de Corpo Estranho/induzido quimicamente , Nanofibras/química , Polietilenoglicóis/farmacologia , Telas Cirúrgicas/veterinária , Animais , Materiais Biocompatíveis/química , Quitosana/química , Modelos Animais de Doenças , Técnicas Eletroquímicas , Reação a Corpo Estranho/patologia , Granuloma de Corpo Estranho/patologia , Hérnia Abdominal/cirurgia , Herniorrafia/instrumentação , Masculino , Teste de Materiais , Polietilenoglicóis/química , Polietilenotereftalatos , Polipropilenos/farmacologia , Ratos , Ratos Wistar , Propriedades de Superfície , Suturas , Resistência à TraçãoRESUMO
Foi atendido no Hospital de Clínicas Veterinária da UFRGS um canino da raça Pitbull, de aproximadamente cinco anos e pesando 40 kg. O animal apresentava evisceração de diversos órgãos abdominais devido a atropelamento, além de choque hipovolêmico.(AU)