RESUMO

Expression of nine genes encoding enzymes involved in the sulfur assimilation pathway was examined by RNA blot hybridization. Significantly increased levels of transcripts encoding ATP sulfurylase and APS reductase were apparent under sulfur deprivation. However, in the absence of nitrogen, their responsiveness to sulfur deprivation was markedly reduced. Results suggest that the sulfur assimilation pathway is regulated at the transcriptional level by both nitrogen and sulfur sources.

Assuntos

Genes de Plantas/genética , Nitrogênio/deficiência , Oxirredutases atuantes sobre Doadores de Grupo Enxofre , Oxirredutases/biossíntese , Plantas Medicinais/enzimologia , Sulfato Adenililtransferase/biossíntese , Enxofre/metabolismo , Colômbia , Escuridão , Luz , Oxirredutases/genética , Plantas Medicinais/genética , RNA de Plantas/biossíntese , RNA de Plantas/genética , Sulfato Adenililtransferase/genética , Enxofre/deficiência , Transcrição Gênica