RESUMO
BACKGROUND: The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions. AIM: To assess the frequency of different HPV genotypes in pre cancerous cervical lesions. MATERIAL AND METHODS: A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism. RESULTS: All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76% of women. Fourteen percent had an infection with multiple subtypes and in 10%, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53. CONCLUSIONS: There is a high rate of infection with HPV with a high oncogenic risk among these women.
Assuntos
DNA Viral/isolamento & purificação , Papillomaviridae/isolamento & purificação , Lesões Pré-Cancerosas/virologia , Displasia do Colo do Útero/virologia , Neoplasias do Colo do Útero/virologia , Esfregaço Vaginal , Adolescente , Adulto , Idoso , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Sondas de DNA de HPV/genética , Sondas de DNA de HPV/isolamento & purificação , Feminino , Genótipo , Humanos , Pessoa de Meia-Idade , Papillomaviridae/classificação , Papillomaviridae/genética , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/genética , Polimorfismo de Fragmento de Restrição , Índice de Gravidade de DoençaRESUMO
Background: The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions. Aim: To assess the frequency of different HPV genotypes in pre cancerous cervical lesions. Material and methods: A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism. Results: All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76 percent of women. Fourteen percent had an infection with multiple subtypes and in 10 percent, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53. Conclusions: There is a high rate of infection with HPV with a high oncogenic risk among these women.
Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Displasia do Colo do Útero/virologia , DNA Viral/isolamento & purificação , Papillomaviridae/isolamento & purificação , Lesões Pré-Cancerosas/virologia , Displasia do Colo do Útero/virologia , Neoplasias do Colo do Útero/virologia , Esfregaço Vaginal , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Sondas de DNA de HPV/genética , Sondas de DNA de HPV/isolamento & purificação , Genótipo , Papillomaviridae/classificação , Papillomaviridae/genética , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/genética , Polimorfismo de Fragmento de Restrição , Índice de Gravidade de DoençaRESUMO
Se realizó una revisión de la s 245 pacientes enviadas a Colposcopia por citología PAP II más cambios virales, en un período de 3 años, se encontró en la biopsia dirigida un 68.16 por ciento de lesión por condiloma, un 31.42 por ciento asociado a NIC y un 0.4 por ciento con cáncer invasor. Esto confirma los informes de la relación de PVH y cáncer de cérvix y por lo tanto la necesidad de colposcopia y biopsia en las pacientes con citologías PAP II y cambios virales (coilocitos, disqueratocitos, binucleación)
Assuntos
Humanos , Feminino , Adulto , Anexina A4 , Anexina A4/isolamento & purificação , Sondas de DNA de HPV , Sondas de DNA de HPV/história , Sondas de DNA de HPV/isolamento & purificação , PapilomaRESUMO
Se analizaron 64 muestras de ADN obtenido de tejido histológicamente normal del cérvix y de lesiones tempranas del cáncer cervical por hibridación dot-blot, para detectar la presencia de papilomavirus humano(PVH). Utilizando ADN de controles adecuados y sondas específicas, detectamos la infección por PVH en 39 por ciento(25 de 64) de los casos; mientras que 61 por ciento (39 de 64) resultó negativo a PVH. Se detectó PVH del grupo de ®bajo riesgo¼ (tipo 6/11) en un 80 por ciento (20 de 25) de las muestras de ADN; mientras que un 8 por ciento (2 de 25) fue positivo a PVH del grupo de ®alto riesgo¼ (tipo 16/18). Además, encontramos 12 por ciento (3 de 25) de las muestras positivas a ambos grupos de virus