RESUMO
Mercury (Hg) pollution has been a global concern in recent decades, posing a significant threat to entire ecosystems and human health due to its cumulative toxicity, persistence, and transport in the atmosphere. The intense interaction between mercury and selenium has opened up a new field for studying mercury removal from industrial flue gas pollutants. Besides the advantages of good Hg° capture performance and low secondary pollution of the mineral selenium compounds, the most noteworthy is the relatively low regeneration temperature, allowing adsorbent regeneration with low energy consumption, thus reducing the utilization cost and enabling recovery of mercury resources. This paper reviews the recent progress of mineral selenium compounds in flue gas mercury removal, introduces in detail the different types of mineral selenium compounds studied in the field of mercury removal, reviews the adsorption performance of various mineral selenium compounds adsorbents on mercury and the influence of flue gas components, such as reaction temperature, air velocity, and other factors, and summarizes the adsorption mechanism of different fugitive forms of selenium species. Based on the current research progress, future studies should focus on the economic performance and the performance of different carriers and sizes of adsorbents for the removal of Hg0 and the correlation between the gas-particle flow characteristics and gas phase mass transfer with the performance of Hg0 removal in practical industrial applications. In addition, it remains a challenge to distinguish the oxidation and adsorption of Hg0 quantitatively.
Assuntos
Poluentes Atmosféricos , Mercúrio , Mercúrio/química , Adsorção , Poluentes Atmosféricos/química , Selênio/química , Gases/química , Compostos de Selênio/químicaRESUMO
In contemporary floriculture, particularly within the cut flower industry, there is a burgeoning interest in innovative methodologies aimed at enhancing the aesthetic appeal and prolonging the postharvest longevity of floral specimens. Within this context, the application of nanotechnology, specifically the utilization of silicon and selenium nanoparticles, has emerged as a promising approach for augmenting the qualitative attributes and extending the vase life of cut roses. This study evaluated the impact of silicon dioxide (SiO2-NPs) and selenium nanoparticles (Se-NPs) in preservative solutions on the physio-chemical properties of 'Black Magic' roses. Preservative solutions were formulated with varying concentrations of SiO2-NPs (25 and 50 mg L-1) and Se-NPs (10 and 20 mg L-1), supplemented with a continuous treatment of 3% sucrose. Roses treated with 20 mg L-1 Se-NPs exhibited the lowest relative water loss, highest solution uptake, maximum photochemical performance of PSII (Fv/Fm), and elevated antioxidative enzyme activities. The upward trajectory of hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels in petals was mitigated by different levels of SiO2 and Se-NPs, with the lowest H2O2 and MDA observed in preservatives containing 50 mg L-1 SiO2- and 20 mg L-1 Se-NPs at the 15th day, surpassing controls and other treatments. Extended vase life and a substantial enhancement in antioxidative capacity were noted under Se and Si nanoparticles in preservatives. The levels of total phenols, flavonoids, and anthocyanin increased during the vase period, particularly in the 50 and 20 mg L-1 Se-NPs and SiO2-NPs. Petal carbohydrate exhibited a declining trend throughout the longevity, with reductions of 8% and 66% observed in 20 mg L-1 Se-NPs and controls, respectively. The longest vase life was achieved with Se-NPs (20 mg L-1), followed by SiO2-NPs (50 mg L-1) up to 16.6 and 15th days, respectively. These findings highlight the significant potential of SiO2- and Se-NPs in enhancing the vase life and physiological qualities of 'Black Magic' roses, with SiO2-NPs showing broad-spectrum efficacy.
Assuntos
Flores , Nanopartículas , Rosa , Selênio , Dióxido de Silício , Selênio/química , Selênio/farmacologia , Dióxido de Silício/química , Nanopartículas/química , Rosa/química , Flores/química , Antioxidantes/química , Antioxidantes/farmacologia , Peróxido de Hidrogênio/metabolismo , Malondialdeído/metabolismoRESUMO
Introduction: Photoaging-induced skin damage leads to appearance issues and dermatoma. Selenium nanoparticles (SeNPs) possess high antioxidant properties but are prone to inactivation. In this study, human serum albumin/SeNPs (HSA-SeNPs) were synthesized for enhanced stability. Methods: HSA-SeNPs were prepared by self-assembling denatured human serum albumin and inorganic selenite. The cytotoxicity of HSA-SeNPs was assessed using the MTT method. Cell survival and proliferation rates were tested to observe the protective effect of HSA-SeNPs on human skin keratinocytes against photoaging. Simultaneously, ICR mice were used for animal experiments. H&E and Masson trichromatic staining were employed to observe morphological changes in skin structure and collagen fiber disorders after UVB irradiation. Quantitative RT-PCR was utilized to measure changes in mRNA expression levels of factors related to collagen metabolism, inflammation, oxidative stress regulation, and senescence markers. Results: The HSA-SeNPs group exhibited significantly higher survival and proliferation rates of UVB-irradiated keratinocytes than the control group. Following UVB irradiation, the back skin of ICR mice displayed severe sunburn with disrupted collagen fibers. However, HSA-SeNPs demonstrated superior efficacy in alleviating these symptoms compared to SeNPs alone. In a UVB-irradiated mice model, mRNA expression of collagen type I and III was dysregulated while MMP1, inflammatory factors, and p21 mRNA expression were upregulated; concurrently Nrf2 and Gpx1 mRNA expression were downregulated. In contrast, HSA-SeNPs maintained the mRNA expression of those factors to be stable In addition, the level of SOD decreased, and MDA elevated significantly in the skin after UVB irradiation, but no significant differences in SOD and MDA levels between the HSA-SeNPs group with UVB irradiation and the UVB-free untreated group. Discussion: HSA-SeNPs have more anti-photoaging effects on the skin than SeNPs, including the protective effects on skin cell proliferation, cell survival, and structure under photoaging conditions. HSA-SeNPs can be used to protect skin from photoaging and repair skin injury caused by UVB exposure.
Assuntos
Proliferação de Células , Sobrevivência Celular , Queratinócitos , Camundongos Endogâmicos ICR , Nanopartículas , Selênio , Envelhecimento da Pele , Pele , Raios Ultravioleta , Animais , Humanos , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Selênio/química , Selênio/farmacologia , Selênio/administração & dosagem , Raios Ultravioleta/efeitos adversos , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Nanopartículas/química , Queratinócitos/efeitos dos fármacos , Queratinócitos/efeitos da radiação , Sobrevivência Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Camundongos , Albumina Sérica Humana/química , Estresse Oxidativo/efeitos dos fármacos , Antioxidantes/farmacologia , Antioxidantes/químicaRESUMO
The rapid variation of influenza challenges vaccines and treatments, which makes an urgent task to develop the high-efficiency and low-toxicity new anti-influenza virus drugs. Selenium is one of the essential trace elements for the human body that possesses a good antiviral activity. In this study, we assessed anti-influenza A virus (H1N1) activity of polyethylene glycol (PEG)-modified gray selenium nanoparticles (PEG-SeNPs) on Madin-Darby Canine Kidney (MDCK) cells in vitro. CCK-8 assay showed that PEG-SeNPs had a protective effect on H1N1-infected MDCK cells. Moreover, PEG-SeNPs significantly reduced the mRNA level of H1N1. TUNEL-DAPI test showed that DNA damage reached a high level but effectively prevented after PEG-SeNPs treatment. Meanwhile, JC-1, Annexin V-FITC and cell cycle assay demonstrated the apoptosis induced by H1N1 was reduced greatly when treated with PEG-SeNPs. Furthermore, the downregulation of p-ATM, p-ATR and P53 protein, along with the upregualation of AKT protein indicated that PEG-SeNPs could inhibit H1N1-induced cell apoptosis through reactive oxygen species (ROS)-mediated related signaling pathways. Finally, Cytokine detection demonstrated PEG-SeNPs inhibited the production of pro-inflammatory factors after infection, including IL-1ß, IL-5, IL-6, and TNF-α. To sum up, PEG-SeNPs might become a new potential anti-H1N1 influenza virus drug due to its antiviral and anti-inflammatory activity.
Assuntos
Apoptose , Vírus da Influenza A Subtipo H1N1 , Polietilenoglicóis , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Cães , Células Madin Darby de Rim Canino , Polietilenoglicóis/química , Polietilenoglicóis/farmacologia , Inflamação/tratamento farmacológico , Antivirais/farmacologia , Selênio/farmacologia , Selênio/química , Espécies Reativas de Oxigênio/metabolismo , Nanopartículas/química , Humanos , Dano ao DNA/efeitos dos fármacosRESUMO
Excessive reactive oxygen species (ROS) is a hallmark of both the onset and progression of inflammatory bowel disease (IBD), where a continuous cycle of ROS and inflammation drives the progression of diseases. The design of oral antioxidant nanoenzymes for scavenging ROS has emerged as a promising strategy to intervene in IBD. However, the practical application of these nanoenzymes is limited due to their single catalytical property and significantly impacted by substantial leakage in the upper gastrointestinal tract. This study introduces a novel oral delivery system, SP@CS-SeNPs, combining natural microalgae Spirulina platensis (SP), which possesses superoxide dismutase (SOD)-like activity, with chitosan-functionalized selenium nanoparticles (CS-SeNPs) that exhibit catalase-like activity. The SP@CS-SeNPs system leverages the dual catalytic capabilities of these components to initiate a cascade reaction that first converts superoxide anion radicals (O2â¢-) into hydrogen peroxide (H2O2), and then catalyzes the decomposition of H2O2 into water and oxygen. This system not only utilizes the resistance of the microalgae carrier to gastric acid and its efficient capture by intestinal villi, thereby enhancing intestinal distribution and retention but also demonstrates significant anti-inflammatory effects and effective repair of the damaged intestinal barrier in a colitis mice model. These results demonstrate that this oral delivery system successfully combines the features of microalgae and nanozymes, exhibits excellent biocompatibility, and offers a novel approach for antioxidant nanozyme intervention in IBD.
Assuntos
Quitosana , Doenças Inflamatórias Intestinais , Microalgas , Selênio , Spirulina , Animais , Camundongos , Selênio/química , Selênio/farmacologia , Doenças Inflamatórias Intestinais/tratamento farmacológico , Doenças Inflamatórias Intestinais/metabolismo , Doenças Inflamatórias Intestinais/patologia , Spirulina/química , Microalgas/química , Administração Oral , Quitosana/química , Portadores de Fármacos/química , Nanopartículas/química , Antioxidantes/química , Antioxidantes/farmacologia , Humanos , Espécies Reativas de Oxigênio/metabolismoRESUMO
Selenium nanoparticles (SeNPs) have garnered extensive research interest and shown promising applications across diverse fields owing to their distinctive properties, including antioxidant, anticancer, and antibacterial activity (Ojeda et al., 2020; Qu et al., 2023; Zambonino et al., 2021, 2023). Among the various approaches employed for SeNP synthesis, green synthesis has emerged as a noteworthy and eco-friendly methodology. Keshtmand et al. (2023) underscored the significance of green-synthesized SeNPs, presenting a compelling avenue in this domain. This innovative strategy harnesses the potential of natural resources, such as plant extracts or microorganisms, to facilitate the production of SeNPs.
Assuntos
Bacillus , Química Verde , Selênio , Selênio/química , Bacillus/metabolismo , Nanopartículas Metálicas/química , Nanopartículas/química , Antioxidantes/químicaRESUMO
Traumatic brain injury (TBI) is an incurable and overwhelming disease accompanied with serve disability and huge financial burden, where the overproduced reactive oxygen species (ROS) can exacerbate the secondary injury, leading to massive apoptosis of neurons. In this study, ß-cyclodextrin (CD)-capped hyperbranched polymers containing selenium element (HSE-CD) were crosslinked with CD-modified hyaluronic acid (HA-CD) and amantadine-modified hyaluronic acid (HA-AD) to obtain a ROS-responsive ointment (R-O). The structures of synthesized polymers were characterized with 1H nuclear magnetic resonance, and the properties of ointment were investigated with rheology and antioxidation. Compared to non-ROS-responsive ointment (N-O), the R-O ointment had stronger efficiency in decreasing the ROS level in BV2 cells in vitro. In a controlled rat cortical impact (CCI) model, the R-O ointment could relieve the DNA damage and decrease apoptosis in injured area via reducing the ROS level. Besides, after the R-O treatment, the rats showed significantly less activated astrocytes and microglia, a lower level of pro-inflammatory cytokines and a higher ratio of M2/M1 macrophage and microglia. Moreover, compared to the TBI group the R-O ointment promoted the doublecortin (DCX) expression and tissue structure integrity around the cavity, and promoted the recovery of nerve function post TBI. STATEMENT OF SIGNIFICANCE: Traumatic brain injury (TBI) is an incurable and overwhelming disease, leading to severe disability and huge social burden, where reactive oxygen species (ROS) are considered as one of the most significant factors in the secondary injury of TBI. A ROS responsive supramolecular ointment containing di-selenide bonds was injected in rats with controlled cortical impact. It relieved the DNA damage and decreased apoptosis in the injured area via reducing the ROS levels, downregulated neuroinflammation, and improved neurological recovery of TBI in vivo. This designed self-adaptive biomaterial effectively regulated the pathological microenvironment in injured tissue, and achieved better therapeutic effect.
Assuntos
Lesões Encefálicas Traumáticas , Pomadas , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio , Selênio , Animais , Selênio/química , Selênio/farmacologia , Lesões Encefálicas Traumáticas/tratamento farmacológico , Lesões Encefálicas Traumáticas/patologia , Masculino , Espécies Reativas de Oxigênio/metabolismo , Ratos , Apoptose/efeitos dos fármacos , Proteína Duplacortina , Linhagem Celular , Camundongos , Microglia/efeitos dos fármacos , Microglia/patologia , Microglia/metabolismoRESUMO
Food-derived nanomaterials optimizing bioactive peptides is an emerging route in the functional food field. Zein and gum arabic (GA) possess favorable encapsulation properties for controlled release, targeted delivery and stabilization of food bioactive ingredients, and thus are considered as promising carriers for delivery systems. In order to improve the bioavailability of rice selenium-containing peptide TSeMMM (T), the nanoparticles (ZTGNs) containing peptide T, zein and GA have been previously prepared. This study focused on evaluating the immunomodulatory capacity of ZTGNs. The results showed that ZTGNs significantly alleviated cyclophosphamide-induced reduction in immune organ indices and liver glutathione content of mice. There was a significant upregulation observed in the levels of immune-related cytokines IL-6, TNF-α, and IFN-γ as well as their mRNA expression. Moreover, ZTGNs enriched the diversity of the intestinal flora and promoted the proportion of beneficial bacteria. In conclusion, ZTGNs have potential as immunomodulatory enhancers for food bioactive ingredients, providing prospects for further optimization of dietary supplements.
Assuntos
Goma Arábica , Nanopartículas , Peptídeos , Selênio , Zeína , Animais , Goma Arábica/farmacologia , Goma Arábica/química , Camundongos , Nanopartículas/química , Selênio/farmacologia , Selênio/química , Zeína/química , Zeína/farmacologia , Peptídeos/farmacologia , Peptídeos/química , Masculino , Microbioma Gastrointestinal/efeitos dos fármacos , Fatores Imunológicos/farmacologia , Fatores Imunológicos/química , Citocinas/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Ciclofosfamida/farmacologiaRESUMO
Selenium nanoparticles (SeNPs) are used in several sectors as antitumor, antimicrobial, and environmental adsorbents. Thus, the present research objective was the production of bacterial-SeNPs as an active and environmentally-friendly antibacterial and adsorbent agents and application into novel nanocomposite filter. From a total of 25 samples (soil, wastewater, and water) obtained from different locations in Egypt, 60 selenium-resistant bacterial isolates were obtained (on a mineral salt medium supplemented with selenium ions). After screening (based on the conversion of selenium from ionic form to nanoform), a superior bacterial isolate for SeNPs formation was obtained and molecular identified as Bacillus pumilus isolate OR431753. The high yield of SeNPs was noted after optimization (glucose as carbon source, pH 9 at 30 °C). The produced SeNPs were characterized as approximately 15 nm-diameter spherical nanoparticles, in addition to the presence of organic substances around these particles like polysaccharides and aromatic amines (protein residues). Also, they have antibacterial activity increased after formation of nanocomposite with nano-chitosan (SeNPs/NCh) against several pathogens. The antibacterial activity (expressed as a diameter of the inhibitory zone) averaged between 2.1 and 4.3, 2.7 and 4.8 cm for SeNPs and SeNPs/NCh, respectively compared with 1.1 to 1.8 cm for Amoxicillin. The produced nanoselenium/chitosan was used as a biofilter to remove mercury (Hg) and AgNPs as model chemicals with serious toxicity and potential pollutant for water bodies in many industries. The new SeNPs/NCh biofilter has proven highly effective in individually removing mercury and AgNPs from their synthetic wastewaters, with an efficiency of up to 99%. Moreover, the removal efficiency of AgNPs stabilized at 99% after treating them with the syringe filter-Se nanocomposite for 4 cycles of treatment (5 min each).
Assuntos
Biodegradação Ambiental , Mercúrio , Nanocompostos , Selênio , Prata , Águas Residuárias , Purificação da Água , Águas Residuárias/química , Nanocompostos/química , Selênio/química , Mercúrio/química , Mercúrio/isolamento & purificação , Purificação da Água/métodos , Prata/química , Poluentes Químicos da Água/química , Nanopartículas Metálicas/química , Antibacterianos/farmacologia , Antibacterianos/química , Desinfecção/métodos , Filtração/métodos , Bacillus/metabolismo , Bacillus/efeitos dos fármacos , Quitosana/química , Quitosana/farmacologiaRESUMO
Helicobacter pylori can infect most people worldwide to cause hazardous consequences to health; the bacteria could not easily be controlled or disinfected. Toward exploring of innovative biocidal nanoformulations to control H. pylori, broccoli seeds (Brassica oleracea var. italica) mucilage (MBS) was employed for biosynthesizing selenium nanoparticles (MBS/SeNPs), which was intermingled with chitosan nanoparticles (NCT) to generate bioactive nanocomposites for suppressing H. pylori. The MBS could effectually generate and stabilize SeNPs with 13.61 nm mean diameter, where NCT had 338.52 nm mean diameter and positively charged (+ 39.62 mV). The cross-linkages between NCT-MBS-SeNPs were verified via infrared analysis and the nanocomposites from NCT:MBS/SeNPs at 1:2 (T1), 1:1 (T2) and 2:1 (T3) ratios had mean diameters of 204, 132 and 159 nm, respectively. The entire nanomaterials/composites exhibited potent anti- H. pylori activities using various assaying methods; the T2 nanocomposite was the utmost bactericidal agent with 0.08-0.10 mg/L minimal concentration and 25.9-27.3 mm inhibition zones. The scanning microscopy displayed the ability of nanocomposite to attach the bacterial cells, disrupt their membranes, and completely lyse them within 10 h. The NCT/MBS/SeNPs nanocomposites provided effectual innovative approach to control H. pylori.
Assuntos
Antibacterianos , Brassica , Quitosana , Helicobacter pylori , Nanocompostos , Mucilagem Vegetal , Selênio , Helicobacter pylori/efeitos dos fármacos , Quitosana/química , Quitosana/farmacologia , Nanocompostos/química , Selênio/química , Selênio/farmacologia , Antibacterianos/farmacologia , Antibacterianos/química , Brassica/microbiologia , Mucilagem Vegetal/química , Nanopartículas/química , Testes de Sensibilidade MicrobianaRESUMO
Intracerebral hemorrhage is a lethal cerebrovascular disease, and the inevitable secondary brain injury (SBI) is responsible for serious disability and death. Perfect therapeutic goal is to minimize SBI and restore neurobehavioral functions. Recently, neuroprotection is highlighted to reduce SBI, but it still faces "Neuronal survival but impaired functions" dilemma. Herein, this work further proposes a novel combinational therapeutic strategy of neuroprotection and neurogenesis toward this goal. However, appropriate therapeutic agents are rarely reported, and their discovery and development are urgently needed. Selenium participates in various physiological/pathological processes, which is hypothesized as a potential targeting molecule. To explore this effect, this work formulates an ultra-small selenium nanodot with a seleno-amino acid derived carbon dot domain and a hydrophilic PEG layer, surprisingly finding that it increases various selenoproteins levels at perihematomal region, to not only exert multiple neuroprotective roles at acute phase but promote neurogenesis and inhibit glial scar formation at recovery phase. At a safe dose, this combinational strategy effectively prevents SBI and recovers neurobehavioral functions to a normal level. Furthermore, its molecular mechanisms are revealed to broaden application scopes in other complex diseases.
Assuntos
Lesões Encefálicas , Acidente Vascular Cerebral Hemorrágico , Fármacos Neuroprotetores , Selênio , Animais , Selênio/química , Selênio/farmacologia , Selênio/uso terapêutico , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/uso terapêutico , Lesões Encefálicas/tratamento farmacológico , Acidente Vascular Cerebral Hemorrágico/tratamento farmacológico , Neurogênese/efeitos dos fármacos , Masculino , Camundongos , Selenoproteínas/metabolismo , Nanopartículas/química , Neurônios/efeitos dos fármacos , Encéfalo/efeitos dos fármacosRESUMO
This study aimed to estimate the hepatic and immune ameliorating potential of extracted bovine lactoferrin (LF), Selenium nanoparticles (SeNPs) or their combination (LF/SeNPs) against bleomycin (BLM) induced hepatic injury. Fifty adult male rats (160-200 g) were equally divided into five groups: (1) the saline control group, (2) BLM-injected (15 mg/kg twice a week, ip), and (3-5) groups treated orally with LF (200 mg/kg/day), SeNPs (0.0486 mg/kg/day) or LF/SeNPs combination (200.0486 mg/kg/day) for 6 weeks post BLM-intoxication. Blood and liver samples were subjected to biochemical, histopathological, and immunohistochemical analyses. The results revealed that BLM caused a significant increase in hepatic lipid peroxidation and nitric oxide, as well as serum markers of liver functions (AST, ALT and GGT activities), and levels of GM-CSF, CD4, TNF-α, IL-1ß, TGF-ß1, fibronectin, triglycerides, cholesterol and LDL-C. Additionally, hepatic glutathione, Na+/K+-ATPase, and glutathione peroxidase, as well as serum HDL-C, total protein and albumin levels were significantly reduced. Moreover, BLM injection resulted in marked histopathological alterations and severe expression of caspase 3. Post-treatment of BLM-intoxicated rats with LF, SeNPs or LF/SeNPs combination obviously improved the BLM-induced hepatic damages; this was achieved from the marked modulations in the mentioned parameters, besides improving the histopathological hepatic architecture. It is worth mentioning that LF/SeNPs exerted the greatest potency. In conclusion, the obtained results demonstrated that LF, SeNPs and LF/SeNPs succeeded in attenuating the BLM-induced hepatic dysfunction. Therefore, these supplements might be used to protect against drug-associated side effects.
Assuntos
Bleomicina , Doença Hepática Induzida por Substâncias e Drogas , Lactoferrina , Fígado , Nanopartículas , Selênio , Lactoferrina/farmacologia , Lactoferrina/administração & dosagem , Animais , Selênio/farmacologia , Selênio/administração & dosagem , Selênio/química , Masculino , Ratos , Bleomicina/efeitos adversos , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Nanopartículas/química , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Ratos WistarRESUMO
A novel selenium dioxide promoted selenylation/cyclization of leucosceptrane sesterterpenoids was reported. Two types of leucosceptrane derivatives with different valence states of selenium atoms (Se2+ and Se4+) were obtained. The mechanisms of these two processes were proposed, and the selenium-containing derivates may serve as intermediates of Riley oxidation that could be trapped with appropriate substrates. Immunosuppressive activity screening revealed that 10 and 11 had obvious inhibitory effects on IFN-γ production, with IC50 values of 5.29 and 17.60 µM, respectively, which were more active than their precursor leucosceptroid A.
Assuntos
Óxidos de Selênio , Sesterterpenos , Ciclização , Óxidos de Selênio/química , Sesterterpenos/química , Sesterterpenos/farmacologia , Interferon gama/metabolismo , Imunossupressores/química , Imunossupressores/farmacologia , Estrutura Molecular , Animais , Camundongos , Selênio/química , Selênio/farmacologiaRESUMO
A recent scientific investigation has shown promising results of selenium nanoparticles (SeNPs) for the anticancer and antimicrobial activities. This study aims to evaluate the effects of PVP SeNPs on bacterial strains, including Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus), Klebsiella pneumoniae (K. pneumoniae), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa). Also, its antitumor activity against the MRC-5 carcinoma cell line. SeNPs were prepared via gamma irradiation using PVP as a capping agent, and their size and morphological structure were determined using HRTEM. The size of the SeNPs ranged from 36 to 66.59 nm. UV-vis spectra confirmed the formation of SeNPs, while FTIR measurement confirmed a change in the PVP structure after adding selenium nanoparticles. The highest effect was reported on HepG2 by an IC50 with a value of 8.87 µg/ml, followed by HeLa, PC3, MCF-7, and Caco2 cell lines, respectively. Furthermore, ZOI reached 36.33 ± 3.05 mm. The best value of the minimum inhibitory concentration (MIC) was 0.313 µg/ml. Scanning electron microscope (SEM) imaging against bacteria showed deformations and distortions in their structures. Transmission electron (TEM) revealed ultrastructure changes in treated bacteria because of the free radicals that made cytotoxicity which confirmed by Electron spin resonance (ESR).
Assuntos
Antibacterianos , Raios gama , Testes de Sensibilidade Microbiana , Selênio , Humanos , Antibacterianos/farmacologia , Antibacterianos/química , Selênio/química , Selênio/farmacologia , Antineoplásicos/farmacologia , Antineoplásicos/química , Linhagem Celular Tumoral , Bactérias/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Bacillus cereus/efeitos dos fármacos , Nanopartículas/química , Pseudomonas aeruginosa/efeitos dos fármacosRESUMO
Stem cells are an essential consideration in the fields of tissue engineering and regenerative medicine. Understanding how nanoengineered biomaterials and mesenchymal stem cells (MSCs) interact is crucial for their role in bone regeneration. Taking advantage of the structural stability of selenium nanoparticles (Se-NPs) and biological properties of natural polymers, Se-NPs-functionalized, injectable, thermoresponsive hydrogels with an interconnected molecular structure were prepared to identify their role in the osteogenic differentiation of different types of mesenchymal stem cells. Further, comprehensive characterization of their structural and biological properties was performed. The results showed that the hydrogels undergo a sol to gel transition with the help of ß-glycerophosphate, while functionalization with Se-NPs significantly enhances their biological response through stabilizing their polymeric structure by forming Se-O covalent bonds. Further results suggest that Se-NPs enhance the differentiation of MSCs toward osteogenic lineage in both the 2D as well as 3D. We demonstrated that the Se-NPs-functionalized hydrogels could enhance the differentiation of osteoporotic bone-derived MSCs. We also focused on specific cell surface marker expression (CD105, CD90, CD73, CD45, CD34) based on the exposure of healthy rats' bone marrow-derived stem cells (BMSCs) to the Se-NP-functionalized hydrogels. This study provides essential evidence for pre-clinical/clinical applications, highlighting the potential of the nanoengineered biocompatible elastic hydrogels for bone regeneration in diseased bone.
Assuntos
Regeneração Óssea , Diferenciação Celular , Quitosana , Colágeno , Hidrogéis , Células-Tronco Mesenquimais , Nanopartículas , Selênio , Hidrogéis/química , Hidrogéis/farmacologia , Regeneração Óssea/efeitos dos fármacos , Animais , Selênio/química , Selênio/farmacologia , Diferenciação Celular/efeitos dos fármacos , Ratos , Nanopartículas/química , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/citologia , Colágeno/química , Quitosana/química , Osteogênese/efeitos dos fármacos , Osteoblastos/efeitos dos fármacos , Osteoblastos/citologia , Ratos Sprague-Dawley , Células CultivadasRESUMO
Heteroatom doping has become an important method to enhance the performance of traditional carbon dots in modern times. Selenium (Se) is a nonmetallic trace element with excellent redox properties and is therefore essential for health. Previous studies have mainly used pure chemicals as selenium sources to prepare selenium-doped carbon dots (Se-CDs), but the precursor pure chemicals have the disadvantages of being expensive, difficult to obtain, toxic, and having low fluorescence yields of the synthesised Se-CDs. Fortunately, our team achieved successful synthesis of selenium carbon dots, exhibiting excellent luminescence and biocompatibility through a one-step hydrothermal method using selenium-enriched natural plant Cardamine, as an alternative to selenium chemicals. This approach aims to address the limitations and high costs associated with Se-CDs precursors. Electron spin resonance spectroscopy (ESR) and cellular antioxidant tests have confirmed the protective ability of Se-CDs against oxidative damage induced by excessive reactive oxygen species (ROS). A new concept and method for synthesizing selenium carbon dots on the basis of biomass, a rationale for the antioxidant effects on human health, and a wide range of development and application possibilities were offered in this work.
Assuntos
Antioxidantes , Carbono , Luminescência , Pontos Quânticos , Selênio , Selênio/química , Carbono/química , Pontos Quânticos/química , Antioxidantes/química , Antioxidantes/farmacologia , Humanos , Espécies Reativas de Oxigênio/metabolismo , Plantas/química , Plantas/metabolismoRESUMO
Herein, the selenium (Se) modified gold nanoparticles (Se-AuNPs) was synthesized using cerium doped carbon dots (Ce-CDs) as a reducing agent and template. As desired, Se-AuNPs displays enhanced peroxidase (POD)-like activity in the presence of Hg2+. The mechanism for the enhanced activity was attributed to the increased affinity between Se-AuNPs-Hg2+ and the substrate, in which Se and Au elements have a strong binding capacity to Hg2+, forming Hg-Se bonds and Au-Hg amalgam to generate more ·OH. This POD-like activity of Se-AuNPs-Hg2+ correlates with the colorimetric reaction by the catalytic reaction between 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2. The oxidation of TMB was completely inhibited by the introduction of the reductive S2-. Based on the above findings, a strategy for the colorimetric detection of Hg2+ and S2- by Se-AuNPs was established with linear ranges of 0.33-66 µg/L and 0.625-75 µg/L, and low detection limits of 0.17 µg/L and 0.12 µg/L (3.3 δ/k), respectively. When the colorimetric probes for detection of Hg2+ and S2- was applied in environmental water samples, the recoveries were in the range of 90.3-108.0 %. This method will provide a new idea for the colorimetric detection strategy of Hg2+ due to the strong interaction between Hg and Se.
Assuntos
Colorimetria , Ouro , Mercúrio , Nanopartículas Metálicas , Selênio , Colorimetria/métodos , Mercúrio/análise , Ouro/química , Nanopartículas Metálicas/química , Selênio/química , Limite de Detecção , Poluentes Químicos da Água/análise , Benzidinas/química , Peroxidase/química , Peroxidase/metabolismo , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/análiseRESUMO
Heavy metals are crucial carcinogenic agents threatening the environment and living habituates. Among them, arsenic (As) is an important metalloid that is categorized as a group I toxic carcinogen. Roxarsone (RX) is an organoarsenic antibiotic compound primarily used as a veterinarian drug and growth promoter for poultry animals. The extensive usage of RX increased the accumulation of As in living beings and the ecosystem. Therefore, we have prepared an electrochemical sensor based on 3D bismuth oxybromide with 2D selenium-doped graphitic carbon nitride (BOB/SCN) electrocatalyst for the rapid detection of RX. The elemental and structural details were thoroughly investigated with several spectroscopic techniques. The electrochemical properties were measured by impedance and voltammetric measurements. The electrocatalytic behavior toward the RX was estimated with different voltammetric methods. Therefore, our BOB/SCN-based electrochemical sensor demonstrated a low detection limit (2.3 nM), low quantification value (7.7 nM), optimal sensitivity (0.675 µA µM-1 cm-2), and good linear ranges (0.01-77 and 77-857 µM). Additionally, this sensor showed good electrochemical performance and was applied to monitor the RX in various real samples with remarkable recoveries. Based on these results, our BOB/SCN sensor is a promising electrochemical platform for determining RX.
Assuntos
Bismuto , Técnicas Eletroquímicas , Grafite , Roxarsona , Selênio , Grafite/química , Técnicas Eletroquímicas/métodos , Catálise , Selênio/química , Bismuto/química , Roxarsona/análise , Roxarsona/química , Animais , Compostos de Nitrogênio/química , Limite de Detecção , Nitrilas/químicaRESUMO
Selenium nanoparticles (SeNPs) are of interest for their versatility and low toxicity, but bare SeNPs are unstable and tend to aggregate and precipitate as black elemental Se, which limits the application of SeNPs. This study evaluated the physicochemical properties, physical stability, antioxidant activities and cytotoxicity of SeNPs stabilized by Grifola frondosa polysaccharides (GFPs) and GFPs-gallic acid conjugates (GFPs-GA). The results showed that the particle size (PZ), polymer index (PDI) and zeta potential (ZP) of the GFPs-SeNPs and GFPs-GA-SeNPs were 58.72 ± 0.53 nm, 0.11, -8.36 ± 0.21 mV and 61.80 ± 0.16 nm, 0.12, -9.37 ± 0.13 mV, respectively. Besides, the GFPs-SeNPs and GFPs-GA-SeNPs remained stable when stored at 4 °C for 70 days in darkness. SeNPs stabilized with GFPs have improved the antioxidant activity and selective toxicity to tumour cells. Interestingly, SeNPs stabilized with GFPs-GA further enhanced these biological activities. This work provided a simple and effective method to construct well-dispersed SeNPs in aqueous systems, demonstrating the important roles of GFPs and GFPs-GA in the size control, dispersion and stabilization of SeNPs. The prepared GFPs-SeNPs and GFPs-GA-SeNPs can serve as good selenium supplements and have potential prospects for antioxidant activity and tumour inhibition.
Assuntos
Antioxidantes , Ácido Gálico , Grifola , Selênio , Selênio/química , Ácido Gálico/química , Grifola/química , Antioxidantes/química , Antioxidantes/farmacologia , Antioxidantes/síntese química , Humanos , Tamanho da Partícula , Polissacarídeos/química , Polissacarídeos/farmacologia , Nanopartículas/química , Sobrevivência Celular/efeitos dos fármacos , Nanopartículas Metálicas/química , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologiaRESUMO
Selenium nanoparticles (SeNPs), as a potential cancer therapeutic agent, have attracted extensive attention due to their high anticancer activity and low toxicity. Polysaccharides could be the modifiers and stabilizers to improve the stability and dispersibility of SeNPs in aqueous solution. This study aimed to investigate the physicochemical characterization, stability, and anti-pancreatic cancer cell activities of SeNPs stabilized by a heteroxylan PVP3-1 extracted from the clusters of Prunella vulgaris Linn. Our results showed that PVP3-1 with Mw of 154 kDa was composed of â4)-ß-D-Xylp(1â, â2, 4)-ß-D-Xylp(1â, t-α-L-Araf(1â and 4-MeO-α-D-GlcpA(1â. Red, zero-valent, and uniform spherical SeNPs with an average diameter of about 60 nm and high stability in aqueous solution were constructed successfully by polysaccharide PVP3-1. Anti-pancreatic cancer cell activity assays showed that PVP3-1-SeNPs could inhibit the proliferation and migration of pancreatic cancer cells in vitro. Furthermore, PVP3-1-SeNPs induced apoptosis and autophagy of pancreatic cancer cells through inhibiting mTOR signaling pathway. In conclusion, these results indicated that PVP3-1-SeNPs could be potential anti-tumor nanoparticles for treating pancreatic cancer.