RESUMO
Two types of cervical mucus are recognized, oestrogenic and gestagenic. These are constituted by different subtypes, and their characteristics change depending on variations in the hormonal levels and on the existence of several pathologies. Our aim was to identify the ultrastructure and crystallization characteristics of the cervical mucus in women suffering from polycystic ovary syndrome, and to compare these characteristics with those of normal control women. Cervical mucus samples were taken from 10 women, 4 control group women (with normal ovulatory menstrual cycles) and 6 suffering from polycystic ovary syndrome (2 with ovulatory and 4 with anovulatory cycles). This mucus was characterized according to its ultrastructure and crystallization. The type of mucus obtained was related to the levels of oestradiol and progesterone present when the samples were taken. As regards mucus ultrastructure, differences were found between the control women and those with polycystic ovary syndrome and anovulatory menstrual cycles. Such variations were evident in the type of mesh and the average diameter of the mucus pores. Mucus crystallization in control women showed the usual oestrogenic disposition: fern-like (L, P2), rectilinear (S) or a hexagonal structure (P6). On the other hand, in women with polycystic ovary syndrome, indefinite mucus crystallizations were found, as well as crystallization patches resembling oestrogenic and gestagenic-like mucus. This study shows that the ultrastructure and crystallization characteristics of the cervical mucus in polycystic ovary syndrome women are different from those of control women. The latter would be dependent on their levels of oestradiol and progesterone.
Assuntos
Muco do Colo Uterino/química , Microscopia Eletrônica de Varredura/métodos , Síndrome do Ovário Policístico/ultraestrutura , Cristalização , Estradiol/urina , Feminino , Humanos , Ciclo Menstrual , Microscopia/métodos , Ovulação , Síndrome do Ovário Policístico/metabolismo , Progesterona/urinaRESUMO
Numerous models have been developed to study polycystic ovarian syndrome in rats. In the present study, the syndrome was induced by exposure to constant light. The histological structure and differential distribution of extracellular matrix (ECM) fibers as well as the glycosaminoglycans (GAGs) content and composition of the ovarian follicular wall of rats with polycystic syndrome were evaluated. Histochemical differences were observed in the graunlosa and theca externa of follicular cysts when compared to normal preovulatory follicles. The colagen content of the theca externa of follicular cysts, quantified by the picrosirius method, was higher than in the controls. The neural carbohydrate and acidic GAC levels were lower in the granulosa and higher in the theca externa of cyst follicles than in control ovaries. Histomorphometrically, the follicular diameter was both a convenient and appropriate measurement for describing the cyst status; there were no differences in the thickness of each follicular layer. In conclusion, differences in the components of ECM were observed in the follicular wall of ovarian cysts compared eith normal preovulatory follicles. Howere, sinde these changes did not occur uniformly in all layers of the follicular wall, their role in cyst development remains to be established.
Assuntos
Animais , Ratos , Matriz Extracelular , Histocitoquímica/métodos , Síndrome do Ovário Policístico/etiologia , Síndrome do Ovário Policístico/fisiopatologia , Síndrome do Ovário Policístico/ultraestrutura , Folículo Ovariano/anormalidades , Folículo Ovariano/citologia , GlicosaminoglicanosRESUMO
Objetivo: Evaluar el efecto del tratamiento endoscópico del síndrome de ovarios poliquísticos en un modelo murino inducido con estrógeno de depósito: Material y métodos: Grupo 1 (control, n = 15) ratas con inhibición de la actividad gonadotrópica y tres grupos con síndrome de ovarios poliquísticos: grupo 2 (n = 22) evaluadas exclusivamante por laparoscopia; grupo 3 (n = 11) fue efectuada una fulguración en cada ovario durante el procedimientos endoscópico; y grupo 4 (n = 10) con tres fulguraciones en cada ovario. Un mes después de la cirugía, los animales fueron sacrificados y se realizó estudio de los órganos intraabdominales mediante microscopio quirúrgico y evaluación histológica de las gónadas. Resultados: Todos los animales tuvieron síndrome de ovarios poliquísticos. Los del grupo 1 no desarrollaron adherencias; mientras que los del grupo 3 las presentaron en 36.3 por ciento de los casos y hubo atrofía gonadal en 18.1 por ciento. El 66.6 por ciento de las ratas del grupo 3 tuvieron adherencias intraabdominales. Hubo diferencia significativa en el grado de formación de adherencias cuando se compararon los grupos 3 y 4 con el grupo 2. Conclusiones: El uso de estrógeno de depósito es útil para la producción del síndrome de ovarios poliquísticos en animales de experimentación. El procedimiento puede emplearse para estudiar los efectos de la inducción quirúrgica de la ovulación; su utilización en otras condiciones también parece ser promisorio
Assuntos
Animais , Ratos , Endoscopia , Endoscopia/tendências , Endoscopia/estatística & dados numéricos , Síndrome do Ovário Policístico/história , Síndrome do Ovário Policístico/metabolismo , Síndrome do Ovário Policístico/ultraestrutura , Indução da Ovulação/métodos , Indução da Ovulação , Laparoscopia , Laparoscopia/história , Laparoscopia/estatística & dados numéricosRESUMO
Os autores descrevem os aspectos morfológicos do ovário de ratas em estro permanente, com ênfase para distribuiçäo dos tipos de colágeno, utilizando o método do Picrosírius-Polarizaçäo. Os dados obtidos säo indicativos de que o colágeno, particularmente os tipos Ie III, säo susceptíveis a hiperplasia na dependência de açäo hormonal