RESUMO
Se estudian los perfiles ribosomales en un miometrio normal en menopausia, y tres miometrios normales al final del embarazo. Se estudian tambien estos perfiles ribosomales en tres miometrios patologicos en adenomiosis, en miomatosis y en sarcomatosis. La mayor actividad en sintesis proteica miometrial fue revelada en orden decreciente por la adenomiosis, la sarcomatosis, la miomatosis, el embarazo a termino y finalmente la menopausia.
Assuntos
Humanos , Células Eucarióticas/análise , Células Eucarióticas/citologia , Células Eucarióticas/patologia , Miométrio , Ribossomos/análise , Ribossomos/fisiologiaRESUMO
Two types of cytoplasmic ribosomes of Euglena gracilis have been found when the stability in vitro of the large subunit at 25 degrees C for 30 min has been considered. Sucrose density gradient analysis have revealed that the large subunit of ribosomes obtained from cells harvested in the stationary phase of growth was degraded when heated at 25 degrees C. The large subunit of ribosomes obtained from exponentially growing cells was stable in the same experimental conditions. Studies of the ribosomal RNAs have not explained this differential stability observed in vitro. In fact, after SDS-extraction, the largest RNA species always appears degraded in both types of ribosomes. Therefore, an explanation to this phenomenon have been searched for through the analysis of the ribosomal proteins. One and two dimensional gel electrophoresis techniques have been employed. At least one difference between the two ribosomal protein groups has been observed and this could be associated to the differential stability of the ribosomes. Approximately 72 proteins molecules have been found in Euglena's cytoplasmic ribosome. About 42 proteins belong to the large subunit and 30 to the small one.
Assuntos
Euglena/análise , Proteínas Ribossômicas/classificação , Animais , Centrifugação com Gradiente de Concentração , Citoplasma/análise , Eletroforese em Gel de Poliacrilamida , Euglena/crescimento & desenvolvimento , Temperatura Alta , Proteínas Ribossômicas/isolamento & purificação , Ribossomos/análiseRESUMO
The cytoplasmic ribosomes from Euglena gracilis var. bacillaris are found to be of two types taking into consideration their stability "in vitro". In the group of unstable ribosomes the large subunit is degraded. The other group apparently does not suffer any degradation under the conditions described. However the RNAs extracted from both types of ribosomes are degraded during sucrose density gradients. The degradation of the largest RNA species has been reported previously, but no comment has been made about the stability of the ribosome itself.