RESUMO
Alcohol abuse constitutes a major cohort among HIV-infected individuals. The precise effect of alcohol addiction on HIV pathogenesis remains inconclusive, however. This study was designed to determine the effect of alcohol dependence on virus replication and CD4 profiles in simian immunodeficiency virus/simian-HIV-infected rhesus macaques. A group of 3 male Indian rhesus macaques was adapted to a self-drinking model of alcohol consumption, whereas another group of 3 macaques was provided a Nutrasweet solution. After 7 weeks of alcohol consumption, the alcohol-dependent animals along with controls were intravenously inoculated with a mixture of SHIV(KU), SHIV(89.6)P, and SIV/17E-Fr. These animals were followed for a period of 24 weeks for complete blood cell counts, CD4 cell profiles, and viral loads in the blood and cerebral compartments. The alcohol and control groups showed comparable peak viral loads in the blood. The plasma viral load in the alcohol group was 31- to 85-fold higher than that in the control group at weeks 18 through 24 after infection, however. The pattern of cerebrospinal fluid viral replication was also comparable during the acute phase; however, the virus continued to replicate in the brain of alcohol-dependent animals, whereas it became undetectable in the controls. The extent of CD4 cell loss in the alcohol group was significantly higher than that in the control animals at week 1 after infection.
Assuntos
Alcoolismo/fisiopatologia , Retrovirus dos Símios/fisiologia , Síndrome de Imunodeficiência Adquirida dos Símios/fisiopatologia , Vírus da Imunodeficiência Símia/fisiologia , Replicação Viral , Alcoolismo/complicações , Alcoolismo/virologia , Animais , Relação CD4-CD8 , Linfócitos T CD4-Positivos/fisiologia , Linfócitos T CD8-Positivos/fisiologia , Macaca mulatta , Masculino , RNA Viral/sangue , RNA Viral/líquido cefalorraquidiano , Síndrome de Imunodeficiência Adquirida dos Símios/complicações , Síndrome de Imunodeficiência Adquirida dos Símios/virologia , Fatores de Tempo , Carga ViralRESUMO
Viral replication, histopathological and ultrastructural changes were observed for a period of nine days in the small intestine of suckling mice infected with a simian rotavirus (SA11). Samples taken from duodenum, jejunum and ileum were prepared for light microscopy, transmission and scanning electron microscopy analysis. Histopathologic effect could be detected within 8 hr post-infection, when only a few altered cells were observed. Damage was extensive after 16 hr post-infection, showing swollen enterocytes and reduced and irregularly oriented microvilli at intestinal villi tips. Virus particles were detected at 16 and 48 hr post-infection, budding from the viroplasm into the rough endoplasmic reticulum cisternae in ileum enterocytes. Clear evidence of viral replication, observed by electron microscopy was not described before in heterologous murine models. Regeneration of the intestinal villi began at the third day post-infection. Despite some differences observed in clinical symptoms and microscopic analysis of homologous and heterologous rotavirus infections, we concluded that mechanisms of heterologous rotavirus infection in mice follow similar patterns to those observed in the homologous models.
Assuntos
Intestinos/virologia , Retrovirus dos Símios/ultraestrutura , Infecções por Rotavirus/virologia , Animais , Intestinos/fisiopatologia , Intestinos/ultraestrutura , Camundongos , Retrovirus dos Símios/crescimento & desenvolvimento , Retrovirus dos Símios/isolamento & purificação , Infecções por Rotavirus/patologia , Replicação ViralRESUMO
Contiene:Serologia de la hepatitis viral,metotos de diagnostico laboratorial en las hepatitis virales, nuevos metodos de diagnostico para el virus de la hepatitis B y C, estado actual de la hepatitis A, mutantes del virus B de la hepatitis y sus implicociones clinicas, infeccion por virus C, hepatitis E, manual de diagnostico y tratamiento de las hepatotias virales