RESUMO

Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.

Assuntos

Neurotoxinas/biossíntese , Neurotoxinas/farmacologia , Venenos de Aranha/química , Venenos de Aranha/farmacologia , Animais , Cálcio/fisiologia , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/fisiologia , Linhagem Celular , Clonagem Molecular , Receptores de Inositol 1,4,5-Trifosfato/biossíntese , Camundongos , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Canal de Liberação de Cálcio do Receptor de Rianodina/biossíntese , Venenos de Aranha/biossíntese , Aranhas/química