RESUMO

G-protein-coupled receptors (GPCRs) play a major role in psychiatric disorders and are the targets of several current therapeutic approaches in this field. A number of studies have now shown that GPCRs can assemble as high molecular weight homo- and hetero-oligomers, which could affect ligand binding, intracellular signalling or trafficking. This information could be critical in design of new drugs to treat neurological and psychiatric disorders. This chapter describes a sequential co-immunoprecipitation and immunoblot protocol for determining oligomerisation of the 5-hydroxytryptamine (HT)1A receptor with other GPCRs in co-transfected HEK-293 cells.

Assuntos

Western Blotting/métodos , Cromatografia de Afinidade/métodos , Imunoprecipitação/métodos , Receptores Acoplados a Proteínas G/análise , Células HEK293 , Humanos , Oligopeptídeos , Multimerização Proteica , Proteínas Proto-Oncogênicas c-myc , Receptor 5-HT1A de Serotonina/análise , Receptor 5-HT1A de Serotonina/química , Receptor 5-HT1B de Serotonina/análise , Receptor 5-HT1B de Serotonina/química , Receptor 5-HT1D de Serotonina/análise , Receptor 5-HT1D de Serotonina/química , Receptores Acoplados a Proteínas G/química , Proteínas Recombinantes de Fusão/análise , Proteínas Recombinantes de Fusão/química , Transfecção