RESUMO

Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells. We expressed human lymphotactin (LTN) by the lactic-acid bacterium Lactococcus lactis. Biological activity of LTN was confirmed by chemo-attraction of human T-cells by chemotaxis demonstrating, for the first time, how this chemokine secreted by a food-grade prokaryote retains biological activity and chemoattracts T lymphocytes. This strain thus represents a feasible well-tolerated vector to deliver active LTN at a mucosal level.

Assuntos

Quimiocinas C/biossíntese , Quimiocinas C/farmacologia , Quimiotaxia/fisiologia , Lactococcus lactis/metabolismo , Linfócitos/efeitos dos fármacos , Linfócitos/fisiologia , Engenharia de Proteínas/métodos , Células Cultivadas , Quimiocinas C/genética , Quimiotaxia/efeitos dos fármacos , Humanos , Lactococcus lactis/genética , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/metabolismo