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Glucosylceramides (GlcCer) are the main neutral glycosphingolipids expressed in fungal cells. In this work, glucosylceramides (GlcCer) were extracted from three strains of Scedosporium (Pseudallescheria) boydii, one strain of Pseudallescheria ellipsoidea and one strain of Pseudallescheria angusta and purified by several chromatographic steps. Using high-performance thin layer chromatography (HPTLC), we found a similarity between GlcCer obtained from all of the analysed strains. A detailed structural analysis of the P. ellipsoidea GlcCer was performed via electrospray ionization mass spectrometry (ESI-MS) and confirmed in 1- and 2-D heteronuclear NMR experiments ((1)H-(13) C HSQC). GlcCer species produced by mycelial forms of these strains displayed the same structure previously demonstrated by our group for P. boydii, Cryptococcus neoformans, Pseudallescheria minustipora, Fusarium solani, and Colletotrichum gloesporioides. A monoclonal antibody (mAb) against GlcCer was used for immunofluorescence experiments. Our results revealed that GlcCer is present on the surface of these fungi, and no difference was observed in the GlcCer structure of the present set of strains in terms of geographic or clinical origin, suggesting a conserved GlcCer structure similar to those previously described for Scedosporium apiospermum, Scedosporium aurantiacum, and P. minutispora. The surface distribution of GlcCer in these fungi is suggestive of the involvement of this molecule in fungal growth.

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El complejo Pseudallescheria/Scedosporium y taxas relacionados, pertenecientes a un grupo de microhongos con conidios viscosos e integrantes del orden Microascales, se presentan en diversos ambientes comunes asociados a las actividades humanas, ya sea en el suelo como en aguas contaminadas. Actualmente se consideran entre los mayores grupos de hongos filamentosos oportunistas causante de infecciones cutáneas y profundas en el hombre y otros mamíferos. El objetivo principal de este trabajo, consiste en reunir los datos primarios morfofisiológicos más relevantes, útiles para el micólogo médico en el laboratorio, con el aporte adicional de algunos aspectos, biológicos, ecológicos, taxonómicos y moleculares complementarios descritos en la literatura moderna.

The Pseudallescheria/Scedosporium complex and their related taxa are a group of fungus that conidia are presents in viscous mass and belong to the order Microascales. They are in several common enviroment related to human activities either in soil as in contaminated water. Nowadays they are considerated one of the most opportunistic group of filamentous fungus that may cause superficial and deep skin mycoses infections in man and other mammalian. The aim of this work is to gather the primary relevant morphophysiological aspects, usefull to the medical mycologist in the laboratory, plus the contribution of some of biological, ecological, taxonomical and moleculars complementary aspects that are describe in modern literature.

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A case of a solitary pulmonary nodule due to Scedosporium apiospermum (Pseudallescheria boydii) is related. A review of the pertinent literature was done and, in addition, similar lesions caused by other opportunistic fungi are commented.

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This paper reports a case of a single subcutaneous nodule caused by Pseudallescheria boydii in a renal transplant recipient, possibly of nontraumatic origin. The patient was treated surgically and with itraconazole.

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Paciente de sexo femenino, 48 años, sin antecedentes clínicos de importancia presenta obstrucción nasal, rinorrea y poliposis. La tomografía axial computadorizada revela masa heterogénea en cavidad maxilar y paranasal derechas, fosa nasal con desviación del tabique y erosión ósea. Se efectúa sinusotomía maxilar derecha y del material se aísla pseudallescheria boydii. Recibe tratamiento con anfotericina B 975 mg hasta tipificación del agente etiológico; continúa con itraconazol 200 mg/día hasta la fecha (9 meses) sin recidivas (AU)

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RESUMO

Paciente de sexo femenino, 48 años, sin antecedentes clínicos de importancia presenta obstrucción nasal, rinorrea y poliposis. La tomografía axial computadorizada revela masa heterogénea en cavidad maxilar y paranasal derechas, fosa nasal con desviación del tabique y erosión ósea. Se efectúa sinusotomía maxilar derecha y del material se aísla pseudallescheria boydii. Recibe tratamiento con anfotericina B 975 mg hasta tipificación del agente etiológico; continúa con itraconazol 200 mg/día hasta la fecha (9 meses) sin recidivas

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Os autores fazem relato de um caso de eumicetoma produzido por Petriellidium boydii. Ao exame direto, observaram-se gräos branco-amarelados, que cultivados em agar Sabouraud, mostraram, à micologia, conídios característicos. Após um mês de cultivo, observaram-se elementos esféricos cujo exame microscópico mostrou cleistotécios abertos e fechados. A paciente foi submetida a diferentes esquemas de tratamento, näo demostrando melhora no quadro. Há um mês, foi alterado o tratamento para ketocconazole, näo havendo ainda, tempo suficiente para observaçäo do seu efeito

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A wide variety of bat inhabitats was surveyed for the presence of Histoplasma capsulatum and other human pathogenic fungi. H. capsulatum was recovered from 9 tree hollows. Four isolates of Allescheria boydii and 7 of Microsporum gypseum were obtained from similar arboreal sites. The significance of these findings is discussed (summary)

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