RESUMO

This article describes a case of horizontal (heterosexual) and subsequent vertical (mother to infant) transmission of 2 human immunodeficiency viruses type 1 (HIV-1) subtypes. Dual infection in a husband, his wife, and their child was initially detected by use of a restriction fragment length polymorphism assay of the proviral protease in peripheral blood mononuclear cells. The simultaneous presence of highly similar sets of HIV-1 subtypes B and C infecting the 3 family members was confirmed by DNA sequence analysis of pol, gag, and env genes. These data, together with available epidemiologic information, may indicate that the husband's high-risk sexual behavior was the source of dual infections. Because his wife did not report such activities, it was likely that he passed HIV-1 strains to his spouse, who subsequently transmitted them to their child.

Assuntos

DNA Viral/análise , Infecções por HIV/transmissão , HIV-1/isolamento & purificação , Adulto , Brasil/epidemiologia , Clonagem Molecular , Transmissão de Doença Infecciosa , Endopeptidases/análise , Feminino , Genes env , Genes gag , Genes pol , Infecções por HIV/epidemiologia , Infecções por HIV/genética , HIV-1/classificação , Humanos , Recém-Nascido , Transmissão Vertical de Doenças Infecciosas , Masculino , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Provírus/enzimologia , Assunção de Riscos