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1.
Braz J Med Biol Res ; 49(2): e5039, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26871971

RESUMO

Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with cornin (10 µM) blocked the reduction of expression of phospho-CREB and phospho-Akt in a hypoxic condition. Treatment of rats with cornin (30 mg/kg, iv) protected them from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics, and reduction of severity of myocardial damage. Cornin treatment also attenuated the reduction of expression of phospho-CREB and phospho-Akt in ischemic myocardial tissue. These data suggest that cornin exerts protective effects due to an increase in expression of phospho-CREB and phospho-Akt.


Assuntos
Proteína de Ligação a CREB/metabolismo , Frutas/química , Glicosídeos Iridoides/farmacologia , Isquemia Miocárdica/tratamento farmacológico , Fitoterapia , Substâncias Protetoras/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Verbena/química , Animais , Western Blotting , Proteína de Ligação a CREB/efeitos dos fármacos , Linhagem Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Creatina Quinase/sangue , Modelos Animais de Doenças , Hipóxia/tratamento farmacológico , Glicosídeos Iridoides/isolamento & purificação , Masculino , Extratos Vegetais/química , Proteínas Proto-Oncogênicas c-akt/efeitos dos fármacos , Ratos Sprague-Dawley , Troponina/sangue
2.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;49(2): e5039, 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-951660

RESUMO

Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with cornin (10 µM) blocked the reduction of expression of phospho-CREB and phospho-Akt in a hypoxic condition. Treatment of rats with cornin (30 mg/kg, iv) protected them from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics, and reduction of severity of myocardial damage. Cornin treatment also attenuated the reduction of expression of phospho-CREB and phospho-Akt in ischemic myocardial tissue. These data suggest that cornin exerts protective effects due to an increase in expression of phospho-CREB and phospho-Akt.


Assuntos
Animais , Masculino , Isquemia Miocárdica/tratamento farmacológico , Verbena/química , Proteína de Ligação a CREB/metabolismo , Glicosídeos Iridoides/farmacologia , Frutas/química , Fitoterapia , Troponina/sangue , Linhagem Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Western Blotting , Ratos Sprague-Dawley , Creatina Quinase/sangue , Modelos Animais de Doenças , Proteína de Ligação a CREB/efeitos dos fármacos , Glicosídeos Iridoides/isolamento & purificação , Hipóxia/tratamento farmacológico
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