RESUMO
A polyethylene-glycol insoluble serum fraction was studied in patients with sickle cell anaemia during the steady state of the disease. The levels of C1q-precipitins were normal but increased amounts of proteins, IgM C3 and factor B were detected in this immune complex enriched serum fraction. These findings are a sign that circulating immune complexes can be detected even in the asymptomatic period of the disease.
Assuntos
Anemia Falciforme/imunologia , Complexo Antígeno-Anticorpo/sangue , Adolescente , Adulto , Anemia Falciforme/sangue , Proteínas Sanguíneas/análise , Criança , Complemento C1q/análise , Humanos , Imunoglobulinas/análise , Precipitinas/análiseAssuntos
Alveolite Alérgica Extrínseca/imunologia , Pulmão do Criador de Aves/imunologia , Adulto , Animais , Pulmão do Criador de Aves/diagnóstico , Pulmão do Criador de Aves/genética , Criança , Pré-Escolar , Doença Crônica , Columbidae/imunologia , Feminino , Humanos , Imunodifusão , Masculino , Pessoa de Meia-Idade , Precipitinas/análiseRESUMO
Investigations were initiated to study the possible detection and measurement of coproantibodies in animals infected with a gastrointestinal nematode parasite. Faecal extracts, extracts of small intestinal mucosa and sera of rats infected with intestinal nematode Nippostrongylus brasiliensis were examined for total IgA, IgM and IgG levels and haemagglutinating and precipitating antibodies specific to parasite antigens over a 30-day-period following infection. It was found that in both faecal and mucosal extracts immunoglobulin concentrations increased after a primary infection. In faecal extracts there was a seven-fold increase of IgA, a three to six-fold increase of IgG and about a fifty-fold increase of IgM. Haemagglutinins in faecal extracts detected by adult worm excretory-secretory (ES) products and adult worm and infective larvae somatic extracts were observed from 3 days after infection (DAI). Haemagglutinins detected by ES products reached their highest titres on 11-12 DAI while those reacting with adult worm somatic extracts showed the highest level between 15 and 19 DAI. A similar pattern of response was found in the antibody levels of the intestinal mucosa. Haemagglutinins detected in faeces during the first 12 DAI reacted with the same antigens as antibodies present in the sera at that time but coproantibodies from 18, 24 and 30 DAI were different from those circulating in sera at that stage of the infection. The results suggest that measurement of coproantibody levels may provide a convenient and useful index of local immune responses to gastrointestinal helminths.
Assuntos
Anticorpos/análise , Fezes/imunologia , Infecções por Nematoides/imunologia , Nippostrongylus/imunologia , Animais , Feminino , Hemaglutininas/análise , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Mucosa Intestinal/imunologia , Precipitinas/análise , RatosAssuntos
Actinomyces/imunologia , Alveolite Alérgica Extrínseca/imunologia , Anticorpos Antibacterianos/análise , Antígenos de Fungos/análise , Columbidae/imunologia , Precipitinas/análise , Microbiologia do Ar , Animais , Exposição Ambiental , Feminino , Humanos , Imunodifusão , Masculino , Porto RicoRESUMO
A six-year-old girl with recurrent urticaria and angioedema, vasculitis, and probable renal disease exhibited marked blood eosinophilia, increased levels of serum IgE, circulating Clq precipitins, and hypocomplementemia with evidence of activation of complement by the classic pathway. Biopsies of skin and muscle revealed heavy infiltrations of the vessel walls with eosinophils. Immunofluorescence studies revealed deposition of IgM, IgE, and C3 in the vessel walls. Exacerbations of the disease were associated with an increase in the eosinophil count and a decrease in the serum levels of C4 and C3. Remission was achieved with corticosteroid therapy. This patient has many features in common with the syndrome of skin lesions, angioedema, and hypocomplementemia recently described in adults.
Assuntos
Complemento C3/deficiência , Proteínas do Sistema Complemento/deficiência , Edema/imunologia , Eosinofilia/imunologia , Urticária/imunologia , Criança , Proteínas do Sistema Complemento/análise , Feminino , Humanos , Imunoglobulina E/análise , Técnicas Imunológicas , Precipitinas/análise , SíndromeRESUMO
Protectins and agglutinins in several organs, fluids and spawn from Argentine terrestrial and fresh-water gastropod species were examined. Differences or analogies with vertebrate immunoglobulin serological behaviour are summarized. Individual or group variability and the evolutionary meaning of the reproductive system-linked and the Ca++ ion-linked protectins are discussed.