RESUMO

Neste artigo realizou-se a avaliação de ovinos mestiços Santa Inês com pododermatite infecciosa, verificando as alterações no leucograma e proteínas de fase aguda. No primeiro experimento, 70 fêmeas foram separadas em três grupos de acordo com o seu escore podal: Grupo controle (G1) com escore 0; Dermatite interdigital (G2) com escore 1 ou 2 e Pododermatite necrosante (G3) com escore 3, 4 ou 5. Durante dois meses observou-se a evolução clínica e efeitos no leucograma em cinco momentos: M1 (dia 0), M2 (dia 15), M3 (dia 30), M4 (dia 45) e M5 (dia 60). A intensidade do resultado do leucograma foi de baixa magnitude, observando-se alterações significativas (p<0,05) como uma leve leucocitose (G3, M4), atribuída a neutrofilia e um discreto aumento no número total de monócitos (G3, M2 e M3) apesar do extenso dano e necrose tecidual existentes na última fase da doença. No segundo experimento, utilizaram-se 105 animais de sete propriedades com objetivo de isolar o agente etiológico e avaliar os efeitos da doença sobre proteína plasmática total e proteínas de fase aguda. Os animais também foram separados em três grupos: controle, dermatite interdigital e pododermatite necrosante. Em todas as propriedades foi realizado o isolamento de Dichelobacter nodosus. Não houve correlação significativa (p<0,05) das diferentes fases da doença sobre as proteínas estudadas, porém a haptoglobina dos grupos com animais doentes apresentou médias superiores ao grupo controle. De acordo com a metodologia utilizada e resultados obtidos, conclui-se que as proteínas de fase aguda estudadas não foram eficientes na caracterização das fases da pododermatite infecciosa ovina e que a resposta leucocitária foi branda, tornando difícil sua utilização para este fim.

In this paper, Santa Inês crossbred sheep with footrot were evaluated, checking the changes on leukogram and the acute phase proteins. In the first experiment, 70 females were divided into three groups according to their podal scores: a control group (G1) with score 0, scald group (G2) with score 1 or 2 and a footrot group (G3) with score 3, 4 or 5. During two months, the clinical course and its effect on leukogram were observed at five moments: M1 (day 0), M2 (day 15), M3 (day 30), M4 (day 45) and M5 (day 60). The intensity of the white blood cell count was low magnitude, observing significant changes (p <0.05) as a mild leukocytosis (G3, M4), attributed to neutrophilia and a slight increase in the total number of monocytes (G3, M2 and M3) despite the extensive damage and tissue necrosis existed during the final stage of the disease. In the second experiment, 105 animals from seven farms were used with the objective of isolating the causative agent and to evaluate the effect of disease on total plasma protein and acute phase proteins. The animals were also divided into three groups: control, scald and footrot. In all farms, the isolation of Dichelobacter nodosus was regarded. For the different stages of the disease on the proteins studied there was no significant correlation (p<0.05) but the haptoglobin in scald and footrot groups showed higher average than the control group. Based on these results, we conclude that the acute phase proteins studied were not efficient in the characterization of the phases of the disease and the leukocyte response was mild, making it difficult to use for this purpose.

Assuntos

RESUMO

Neste artigo realizou-se a avaliação de ovinos mestiços Santa Inês com pododermatite infecciosa, verificando as alterações no leucograma e proteínas de fase aguda. No primeiro experimento, 70 fêmeas foram separadas em três grupos de acordo com o seu escore podal: Grupo controle (G1) com escore 0; Dermatite interdigital (G2) com escore 1 ou 2 e Pododermatite necrosante (G3) com escore 3, 4 ou 5. Durante dois meses observou-se a evolução clínica e efeitos no leucograma em cinco momentos: M1 (dia 0), M2 (dia 15), M3 (dia 30), M4 (dia 45) e M5 (dia 60). A intensidade do resultado do leucograma foi de baixa magnitude, observando-se alterações significativas (p<0,05) como uma leve leucocitose (G3, M4), atribuída a neutrofilia e um discreto aumento no número total de monócitos (G3, M2 e M3) apesar do extenso dano e necrose tecidual existentes na última fase da doença. No segundo experimento, utilizaram-se 105 animais de sete propriedades com objetivo de isolar o agente etiológico e avaliar os efeitos da doença sobre proteína plasmática total e proteínas de fase aguda. Os animais também foram separados em três grupos: controle, dermatite interdigital e pododermatite necrosante. Em todas as propriedades foi realizado o isolamento de Dichelobacter nodosus. Não houve correlação significativa (p<0,05) das diferentes fases da doença sobre as proteínas estudadas, porém a haptoglobina dos grupos com animais doentes apresentou médias superiores ao grupo controle. De acordo com a metodologia utilizada e resultados obtidos, conclui-se que as proteínas de fase aguda estudadas não foram eficientes na caracterização das fases da pododermatite infecciosa ovina e que a resposta leucocitária foi branda, tornando difícil sua utilização para este fim.(AU)

In this paper, Santa Inês crossbred sheep with footrot were evaluated, checking the changes on leukogram and the acute phase proteins. In the first experiment, 70 females were divided into three groups according to their podal scores: a control group (G1) with score 0, scald group (G2) with score 1 or 2 and a footrot group (G3) with score 3, 4 or 5. During two months, the clinical course and its effect on leukogram were observed at five moments: M1 (day 0), M2 (day 15), M3 (day 30), M4 (day 45) and M5 (day 60). The intensity of the white blood cell count was low magnitude, observing significant changes (p <0.05) as a mild leukocytosis (G3, M4), attributed to neutrophilia and a slight increase in the total number of monocytes (G3, M2 and M3) despite the extensive damage and tissue necrosis existed during the final stage of the disease. In the second experiment, 105 animals from seven farms were used with the objective of isolating the causative agent and to evaluate the effect of disease on total plasma protein and acute phase proteins. The animals were also divided into three groups: control, scald and footrot. In all farms, the isolation of Dichelobacter nodosus was regarded. For the different stages of the disease on the proteins studied there was no significant correlation (p<0.05) but the haptoglobin in scald and footrot groups showed higher average than the control group. Based on these results, we conclude that the acute phase proteins studied were not efficient in the characterization of the phases of the disease and the leukocyte response was mild, making it difficult to use for this purpose.(AU)

Assuntos

RESUMO

Background: Circadian rhythms are biological rhythms generated by an organism or group of organisms that have an intrinsic period of 24 h. Among the many variables that exhibit circadian rhythmicity, body temperature has received considerable attention. The circadian rhythm of body temperature is a well-documented physiological phenomen. Also, it has been shown that changes in heat loss via convection and radiation are primarily caused by variations in skin blood flow, with consequent changes in skin temperature. For this reason, foot temperature assumes a great importance both as indicator of equine laminitis and foot-and-mouth disease in sheep. Particularly, the foot rot, term loosely used to describe lameness associated with the bovine foot, is characterized by acute inflammation of the skin and adjacent soft tissue of the interdigital cleft or space in which the modifications of temperature assume a great importance. In fact, the regulation of body temperature is an essential component of the process of fever, which plays an important role in an organism's response to infection and disease, and its manipulation is a standard procedure in various surgical and therapeutic procedures. In this regards, the aim of this study was to evaluate the pattern of rectal temperature (RT) both in healthy Comisana sheep and in Comisana sheep affected by foot rot. Materials, Methods & Results: This experiment was carried out on 10 clinically healthy and not pregnant female sheep, and 10 not pregnant female sheep affected by foot rot. During the experimental period, all animals were kept under a natural photoperiod (sunrise 06:30, sunset 19:00). RT was measured every 3 h intervals for 24 consecutive hours starting at 11:00 on day 1 and ending at 11:00 on day 2. A trigonometric statistical model was applied to the average values of each time series, so as to describe the periodic phenomenon analytically, by characterizing the main rhythmic parameters according to the single cosinor procedure. Four rhythmic parameters were determined: mean level, amplitude (the difference between the peak, or trough, and the mean value of a wave), acrophase (the time at which the peak of a rhythm occurs), and robustness (strength of rhythmicity). The application of the periodic model showed a circadian rhythm of RT in healthy subjects and no circadian rhytmicity of RT was observed in ill sheep. Discussion: The analysis of the results obtained under experimental conditions used in the present study indicated the existence of a circadian periodicity of rectal temperature in healthy sheep whereas no circadian oscillations of rectal temperature were revealed in sheep affected by foot rot. A simple and coherent explanation for these pathological alterations is that the set point is elevated during the rising phase and returned to normal during the later phase. Taking together, the findings of the present study, along with those of others, emphasize the concept that RT exhibits a circadian rhythm that reflect the familiar circadian patterns of endogenous source and the existence of circadian oscillations around a set point with changes in mean body temperature and an absent rhythm when temperature is modified to a new set point, as observed in sheep affected by foot rot.

Assuntos