RESUMO

The use of King A and King B media for the faster diagnosis of Pseudomonas aeruginosa is introduced in the Microbiology Laboratory of Centro Habana Pediatric Teaching Hospital. The appropriate use of these media allows to classify as Pseudomonas aeruginosa 77.2% of the Pseudomonas sp. strains studied, which represent 95.2% of the total of strains identified as Pseudomonas aeruginosa, according to Hugh and Gilardi conventional technique. There are no significant differences in the implementation of the two procedures (X2: 0.5; p less than 0.05). The demonstrations of pyocines with King A medium is significantly superior to the method for extraction and solubility of the pigment in chloroform (X2: 15.05; p less than 0.01).

Assuntos

Meios de Cultura , Oligopeptídeos , Infecções por Pseudomonas/diagnóstico , Pseudomonas aeruginosa/isolamento & purificação , Técnicas de Tipagem Bacteriana/métodos , Cuba , Hospitais Pediátricos , Hospitais de Ensino , Humanos , Pigmentos Biológicos/análise , Pigmentos Biológicos/biossíntese , Pseudomonas/classificação , Pseudomonas aeruginosa/metabolismo , Piocianina/análise , Piocianina/biossíntese