RESUMO
Long non-coding RNAs (lncRNAs) have emerged as important regulators of many biological processes, although their regulatory roles remain poorly characterized in woody plants, especially in gymnosperms. A major challenge of working with lncRNAs is to assign functional annotations, since they have a low coding potential and low cross-species conservation. We utilised an existing RNA-Sequencing resource and performed short RNA sequencing of somatic embryogenesis developmental stages in Norway spruce (Picea abies L. Karst). We implemented a pipeline to identify lncRNAs located within the intergenic space (lincRNAs) and generated a co-expression network including protein coding, lincRNA and miRNA genes. To assign putative functional annotation, we employed a guilt-by-association approach using the co-expression network and integrated these results with annotation assigned using semantic similarity and co-expression. Moreover, we evaluated the relationship between lincRNAs and miRNAs, and identified which lincRNAs are conserved in other species. We identified lincRNAs with clear evidence of differential expression during somatic embryogenesis and used network connectivity to identify those with the greatest regulatory potential. This work provides the most comprehensive view of lincRNAs in Norway spruce and is the first study to perform global identification of lincRNAs during somatic embryogenesis in conifers. The data have been integrated into the expression visualisation tools at the PlantGenIE.org web resource to enable easy access to the community. This will facilitate the use of the data to address novel questions about the role of lincRNAs in the regulation of embryogenesis and facilitate future comparative genomics studies.
Assuntos
Regulação da Expressão Gênica de Plantas , Picea , RNA Longo não Codificante , Picea/genética , Picea/embriologia , Picea/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas/genética , RNA Longo não Codificante/genética , MicroRNAs/genética , Técnicas de Embriogênese Somática de Plantas/métodos , RNA de Plantas/genética , Anotação de Sequência Molecular , Redes Reguladoras de Genes/genéticaRESUMO
Plant defence mechanisms, including physical barriers like toughened bark and chemical defences like allelochemicals, are essential for protecting them against pests. Trees allocate non-structural carbohydrates (NSCs) to produce secondary metabolites like monoterpenes, which increase during biotic stress to fend off pests like the Eurasian spruce bark beetle, ESBB (Ips typographus). Despite these defences, the ESBB infests Norway spruce, causing significant ecological damage by exploiting weakened trees and using pheromones for aggregation. However, the mechanism of sensing and resistance towards host allelochemicals in ESBB is poorly understood. We hypothesised that the exposure of ESBB to spruce allelochemicals, especially monoterpenes, leads to an upsurge in the important detoxification genes like P450s, GSTs, UGTs, and transporters, and at the same time, genes responsible for development must be compromised. The current study demonstrates that exposure to monoterpenes like R-limonene and sabiene effectively elevated detoxification enzyme activities. The differential gene expression (DGE) analysis revealed 294 differentially expressed (DE) detoxification genes in response to R-limonene and 426 DE detoxification genes in response to sabiene treatments, with 209 common genes between the treatments. Amongst these, genes from the cytochrome P450 family 4 and 6 genes (CP4 and CP6), esterases, glutathione S-transferases family 1 (GSTT1), UDP-glucuronosyltransferase 2B genes (UDB), and glucose synthesis-related dehydrogenases were highly upregulated. We further validated 19 genes using RT-qPCR. Additionally, we observed similar high expression levels of detoxification genes across different monoterpene treatments, including myrcene and α-pinene, suggesting a conserved detoxification mechanism in ESBB, which demands further investigation. These findings highlight the potential for molecular target-based beetle management strategies targeting these key detoxification genes.
Assuntos
Besouros , Inativação Metabólica , Monoterpenos , Picea , Animais , Monoterpenos/metabolismo , Monoterpenos/farmacologia , Picea/metabolismo , Picea/genética , Besouros/metabolismo , Besouros/genética , Besouros/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas , Sistema Enzimático do Citocromo P-450/metabolismo , Sistema Enzimático do Citocromo P-450/genética , Casca de Planta/química , Casca de Planta/metabolismoRESUMO
The rhizosphere is the hotspot for microbial enzyme activities and contributes to carbon cycling. Precipitation is an important component of global climate change that can profoundly alter belowground microbial communities. However, the impact of precipitation on conifer rhizospheric microbial populations has not been investigated in detail. In the present study, using high-throughput amplicon sequencing, we investigated the impact of precipitation on the rhizospheric soil microbial communities in two Norway Spruce clonal seed orchards, Lipová Lhota (L-site) and Prenet (P-site). P-site has received nearly double the precipitation than L-site for the last three decades. P-site documented higher soil water content with a significantly higher abundance of Aluminium (Al), Iron (Fe), Phosphorous (P), and Sulphur (S) than L-site. Rhizospheric soil metabolite profiling revealed an increased abundance of acids, carbohydrates, fatty acids, and alcohols in P-site. There was variance in the relative abundance of distinct microbiomes between the sites. A higher abundance of Proteobacteria, Acidobacteriota, Ascomycota, and Mortiellomycota was observed in P-site receiving high precipitation, while Bacteroidota, Actinobacteria, Chloroflexi, Firmicutes, Gemmatimonadota, and Basidiomycota were prevalent in L-site. The higher clustering coefficient of the microbial network in P-site suggested that the microbial community structure is highly interconnected and tends to cluster closely. The current study unveils the impact of precipitation variations on the spruce rhizospheric microbial association and opens new avenues for understanding the impact of global change on conifer rizospheric microbial associations.
Assuntos
Microbiota , Picea , Rizosfera , Microbiologia do Solo , Picea/microbiologia , Microbiota/genética , Bactérias/genética , Bactérias/classificação , Bactérias/metabolismo , Solo/química , Chuva , Sementes/crescimento & desenvolvimento , Sementes/microbiologia , Mudança ClimáticaRESUMO
Population genetics theory predicts a relationship between fitness, genetic diversity (H0) and effective population size (Ne), which is often tested through heterozygosity-fitness correlations (HFCs). We tested whether population and individual fertility and heterozygosity are correlated in two endangered Mexican spruces (Picea martinezii and Picea mexicana) by combining genomic, demographic and reproductive data (seed development and germination traits). For both species, there was a positive correlation between population size and seed development traits, but not germination rate. Individual genome-wide heterozygosity and seed traits were only correlated in P. martinezii (general-effects HFC), and none of the candidate single nucleotide polymorphisms (SNPs) associated with individual fertility showed heterozygote advantage in any species (no local-effects HFC). We observed a single and recent (c. 30 thousand years ago (ka)) population decline for P. martinezii; the collapse of P. mexicana occurred in two phases separated by a long period of stability (c. 800 ka). Recruitment always contributed more to total population census than adult trees in P. mexicana, while this was only the case in the largest populations of P. martinezii. Equating fitness to either H0 or Ne, as traditionally proposed in conservation biology, might not always be adequate, as species-specific evolutionary factors can decouple the expected correlation between these parameters.
Assuntos
Evolução Biológica , Espécies em Perigo de Extinção , Florestas , Aptidão Genética , Reprodução , Sementes , Árvores , Árvores/genética , Árvores/fisiologia , Sementes/genética , Sementes/fisiologia , Sementes/crescimento & desenvolvimento , Reprodução/genética , Picea/genética , Picea/fisiologia , Picea/crescimento & desenvolvimento , Heterozigoto , Germinação/genética , Polimorfismo de Nucleotídeo Único/genética , Densidade Demográfica , Variação GenéticaRESUMO
Defoliation by eastern spruce budworm is one of the most important natural disturbances in Canadian boreal and hemi-boreal forests with annual area affected surpassing that of fire and harvest combined, and its impacts are projected to increase in frequency, severity, and range under future climate scenarios. Deciding on an active management strategy to control outbreaks and minimize broader economic, ecological, and social impacts is becoming increasingly important. These strategies differ in the degree to which defoliation is suppressed, but little is known about the downstream consequences of defoliation and, thus, the implications of management. Given the disproportionate role of headwater streams and their microbiomes on net riverine productivity across forested landscapes, we investigated the effects of defoliation by spruce budworm on headwater stream habitat and microbiome structure and function to inform management decisions. We experimentally manipulated a gradient of defoliation among 12 watersheds during a spruce budworm outbreak in the Gaspésie Peninsula, Québec, Canada. From May through October of 2019-2021, stream habitat (flow rates, dissolved organic matter [DOM], water chemistry, and nutrients), algal biomass, and water temperatures were assessed. Bacterial and fungal biofilm communities were examined by incubating six leaf packs for five weeks (mid-August to late September) in one stream reach per watershed. Microbiome community structure was determined using metabarcoding of 16S and ITS rRNA genes, and community functions were examined using extracellular enzyme assays, leaf litter decomposition rates, and taxonomic functional assignments. We found that cumulative defoliation was correlated with increased streamflow rates and temperatures, and more aromatic DOM (measured as specific ultraviolet absorbance at 254 nm), but was not correlated to nutrient concentrations. Cumulative defoliation was also associated with altered microbial community composition, an increase in carbohydrate biosynthesis, and a reduction in aromatic compound degradation, suggesting that microbes are shifting to the preferential use of simple carbohydrates rather than more complex aromatic compounds. These results demonstrate that high levels of defoliation can affect headwater stream microbiomes to the point of altering stream ecosystem productivity and carbon cycling potential, highlighting the importance of incorporating broader ecological processes into spruce budworm management decisions.
Assuntos
Ecossistema , Microbiota , Rios , Animais , Quebeque , Mariposas/fisiologia , Picea , LarvaRESUMO
Northern temperate coniferous forests serve as crucial connectors between boreal and temperate forests, yet they are vulnerable to various stressors such as climate change and human activities. Severe drought poses a significant threat to plant species within these forests, prompting recent research into its impacts. However, many studies lack explicit definitions of post-disturbance vegetation processes and fail to identify potential interactions with disturbance factors, necessitating comprehensive discussions. This study examines the effects of drought on tree growth patterns of the main dominant species in northern temperate regions: Abies nephrolepis and Picea jezoensis, along with two commonly associated Betula ermanii, and Quercus mongolica. Additionally, new disturbance factors in forests inhabited by these species (A. nephrolepis and P. jezoensis) were evaluated based on community classification. The study sites were located in the Mt. Baekdu (Changbai) and South Korea regions, which are positioned at the southern limit of the phytogeographical patterns of target species. Results indicate that A. nephrolepis and P. jezoensis exhibit high levels of recovery and resilience, while B. ermanii and Q. mongolica demonstrate high resistance. Species-specific responses align with drought intensity, with resistance, recovery, and resilience decreasing notably with increasing pre-drought radial growth. South Korean forests, the invasion of the vine species Tripterygium regelii after the death of A. nephrolepis in the overstory vegetation threatens the regeneration of new trees. However, certain environmental factors, such as high rock exposure and dense overstory canopy, limit vine invasion. Based on the results, pre-drought radial growth emerges as a key determinant in how trees respond to drought. Additionally, the results suggest the potential for new disturbances to emerge in forest gaps due to overstory vegetation mortality induced by global warming. These findings contribute to a deeper understanding of increasing drought stress, aid in identifying climate refugia, and inform conservation priorities based on habitat characteristics.
Assuntos
Mudança Climática , Secas , Florestas , Árvores , Árvores/fisiologia , República da Coreia , Espécies Introduzidas , China , Quercus/fisiologia , Quercus/crescimento & desenvolvimento , Picea/fisiologia , Picea/crescimento & desenvolvimento , Abies/fisiologiaRESUMO
Forests globally are experiencing severe droughts, leading to significant reductions in growth, crown dieback and even tree mortality. The ability of forest ecosystems to acclimate to prolonged and repeated droughts is critical for their survival with ongoing climate change. In a five-year throughfall exclusion experiment, we investigated the long-term physiological and morphological acclimation of mature Norway spruce (Picea abies [L.] KARST.) and European beech (Fagus sylvatica L.) to repeated summer drought at the leaf, shoot and whole tree level. Throughout the drought period, spruce reduced their total water use by 70 % to only 4-9 L per day and tree, while beech was less affected with about 30 % reduction of water use. During the first two summers, spruce achieved this by closing their stomata by up to 80 %. Additionally, from the second drought summer onwards, spruce produced shorter shoots and needles, resulting in a stepwise reduction of total leaf area of over 50 % by the end of the experiment. Surprisingly, no premature leaf loss was observed. This reduction in leaf area allowed a gradual increase in stomatal conductance. After the five-year drought experiment, water consumption per leaf area was the same as in the controls, while the total water consumption of spruce was still reduced. In contrast, beech showed no significant reduction in whole-tree leaf area, but nevertheless reduced water use by up to 50 % by stomatal closure. If the restriction of transpiration by stomatal closure is sufficient to ensure survival of Norway spruce during the first drought summers, then the slow but steady reduction in leaf area will ensure successful acclimation of water use, leading to reduced physiological drought stress and long-term survival. Neighboring beech appeared to benefit from the water-saving strategy of spruce by using the excess water.
Assuntos
Aclimatação , Mudança Climática , Secas , Fagus , Picea , Folhas de Planta , Estações do Ano , Fagus/fisiologia , Picea/fisiologia , Aclimatação/fisiologia , Folhas de Planta/fisiologia , Água , Estômatos de Plantas/fisiologiaRESUMO
The impact of atmospheric pollution on the growth of European forest tree species, particularly European beech, Silver fir and Norway spruce, is examined in five mesic forests in the Czech Republic. Analyzing of basal area increment (BAI) patterns using linear mixed effect models reveals a complex interplay between atmospheric nitrogen (N) and sulphur (S) deposition, climatic variables and changing CO2 concentrations. Beech BAI responds positively to N deposition (in tandem with air CO2 concentration), with soil phosphorus (P) availability emerging as a significant factor influencing overall growth rates. Fir BAI, on the other hand, was particularly negatively influenced by S deposition, although recent growth acceleration suggests growth resilience in post-pollution period. This fir growth surge likely coincides with stimulation of P acquisition following the decline of acidic pollution. The consequence is the current highest productivity among the studied tree species. The growth dynamics of both conifers were closely linked to the stoichiometric imbalance of phosphorus in needles, indicating the possible sensitivity of exogenous controls on nutrient uptake. Furthermore, spruce BAI was positively linked to calcium availability across sites. Despite enhanced water-use efficiency under elevated CO2, spruce growth is constrained by precipitation deficit and demonstrates weakening resilience to increasing growing season air temperatures. Overall, these findings underscore the intricate relationships between atmospheric pollution, nutrient availability, and climatic factors in shaping the growth dynamics of European forest ecosystems. Thus, incorporating biogeochemical context of nutrient availability is essential for realistic modelling of tree growth in a changing climate.
Assuntos
Poluentes Atmosféricos , Poluição do Ar , Monitoramento Ambiental , Fagus , Picea , Árvores , República Tcheca , Poluição do Ar/estatística & dados numéricos , Poluentes Atmosféricos/análise , Picea/crescimento & desenvolvimento , Fagus/efeitos dos fármacos , Fagus/crescimento & desenvolvimento , Fósforo/análise , Florestas , Nitrogênio , AbiesRESUMO
Quantification mercury (Hg) pools in forests is crucial for understanding the Hg assimilation, flux and even biogeochemical cycle in forest ecosystems. While several investigations focused on Hg pools among broad-leaved, coniferous and mixed forests, there was still absent information on alpine forest. We sampled soil, moss and various tissues of the dominant Qinghai spruce (Picea crassifolia Kom.) to investigate Hg concentrations and pools, and assess Hg accumulation dynamics in the Qilian Mountains, northwestern China. The mean Hg concentration increased in the following order: trunk wood (1.8 ± 0.7 ng g-1) < branch (4.6 ± 0.8 ng g-1) < root (12.2 ± 2.9 ng g-1) < needle (19.3 ± 5.6 ng g-1) < bark (28.7 ± 9.0 ng g-1) < soil (34.1 ± 7.7 ng g-1) < litterfall (42.9 ± 2.9 ng g-1) < moss (62.5 ± 5.0 ng g-1). The soil contained Hg pools two orders of magnitude higher than vegetation and accounted for 92.2 % of the total Hg pool in the alpine forest ecosystem. Moss, despite representing only 2.7 % of total vegetation biomass, contained a disproportionate 16.7 % of the Hg pool. Although species-specific, aboveground spruce tissues exhibited higher Hg pools in alpine forests compared to other forests in China and America. The dynamic accumulation indicated that increasing atmospheric Hg concentration and enhancing tree productivity contributed to rising Hg assimilation in remote alpine forests, particularly after the 1960s. Our results highlight the relatively high levels of Hg pools in aboveground tree tissues of alpine forest and reveal a significant increase in Hg accumulation. We recommend that when assessing Hg dynamics in forest ecosystems, it is crucial to consider both the variability in atmospheric Hg exposure levels and the forest productivity.
Assuntos
Monitoramento Ambiental , Florestas , Mercúrio , Mercúrio/análise , Mercúrio/metabolismo , China , Picea/metabolismo , Árvores , Poluentes Atmosféricos/análise , Poluentes do Solo/análise , Poluentes do Solo/metabolismo , Solo/químicaRESUMO
We present a database of Norway spruce phenotypic traits measured over a period of 51 years in provenance trials planted across western Germany. These trials are part of the 'Inventory Provenance Test 1964/68 with Norway spruce' (IPTNS) that tested a total of 1,100 provenances collected across Europe, both from the species natural and artificial range. Phenotypic traits (first height, later diameter at breast height, survival) were recorded at 33 trial sites of 100 provenances, each a subsample from the entire collection area that represents an approximately comparable climate space. The dataset contains 424,781 records of the same 65,518 trees ranging from age 7 to 51. Overall, it captures the considerable genetic and phenotypic intraspecific variation present in Norway spruce and should be of interest to various fields including quantitative genetics, ecology, biogeography and the adaptive management of forests.
Assuntos
Fenótipo , Picea , Picea/genética , Alemanha , ÁrvoresRESUMO
Most tree species underwent cycles of contraction and expansion during the Quaternary. These cycles led to an ancient and complex genetic structure that has since been affected by extensive gene flow and by strong local adaptation. The extent to which hybridization played a role in this multi-layered genetic structure is important to be investigated. To study the effect of hybridization on the joint population genetic structure of two dominant species of the Eurasian boreal forest, Picea abies and P. obovata, we used targeted resequencing and obtained around 480 K nuclear SNPs and 87 chloroplast SNPs in 542 individuals sampled across most of their distribution ranges. Despite extensive gene flow and a clear pattern of Isolation-by-Distance, distinct genetic clusters emerged, indicating the presence of barriers and corridors to migration. Two cryptic refugia located in the large hybrid zone between the two species played a critical role in shaping their current distributions. The two species repeatedly hybridized during the Pleistocene and the direction of introgression depended on latitude. Our study suggests that hybridization helped both species to overcome main shifts in their distribution ranges during glacial cycles and highlights the importance of considering whole species complex instead of separate entities to retrieve complex demographic histories.
Assuntos
Fluxo Gênico , Genética Populacional , Hibridização Genética , Picea , Polimorfismo de Nucleotídeo Único , Picea/genética , Polimorfismo de Nucleotídeo Único/genética , Noruega , DNA de Cloroplastos/genética , Evolução Biológica , Análise de Sequência de DNARESUMO
Authorities have long proved the utility of bioindicators in monitoring the state of environmental pollution. Some biological indicators can measure environmental pollutant levels, and many tree species have been tested for suitability for monitoring purposes. The differences in morphological characteristics in the trees have demonstrated the effects of human activities on different materials. Measuring bark and wood biomass from contaminated sites was identified and directly compared with those from a clean site or areas characterized by distinct contamination sources. However, preliminary results demonstrate the approach's potential in the realization of strategies for disease control and promoting health to reduce environmental and health inequalities in at-risk urban areas. Picea orientalis L. and Cedrus atlantica Endl., especially their bark, can be regarded as a more robust storage of Cu (37.95 mg/kg) and Mn (188.25 mg/kg) than Pinus pinaster, Cupressus arizonica, and Pseudotsuga menziesii, which and is therefore a better bioindicator for Cu and Mn pollution. Considering the total concentrations as a result of the study, the pollution is thought to be caused by environmental problems and traffic in the region. The deposition of Cu, Mn, Ni, and Zn elements was found P. menziesii (60, 443, 58, and 258 mg/kg) and P. orientalis (76, 1684, 41, and 378 mg/kg) and seems to reflect atmospheric quite clearly compared to P. pinaster, C. arizonica, and C. atlantica. Ni and Zn concentrations have significantly increased since 1983, and P. menziesii and P. orientalis can be potentially valuable bioindicators for emphasizing polluted fields.
Assuntos
Poluentes Atmosféricos , Monitoramento Ambiental , Casca de Planta , Monitoramento Ambiental/métodos , Poluentes Atmosféricos/análise , Casca de Planta/química , Árvores , Metais Pesados/análise , Monitoramento Biológico/métodos , Cidades , Picea/químicaRESUMO
The osmotic resistance mechanism has been extensively studied in whole plants or plant tissues. However, little is known about it in embryogenic tissue (ET) which is widely used in plant-based biotechnological systems. Suberin, a cell wall aliphatic and aromatic heteropolymer, plays a critical role in plant cells against osmosis stress. The suberin regulatory biosynthesis has rarely been studied in gymnosperms. Here, PaMYB11, a subgroup 11 R2R3-MYB transcription factor, plays a key role in the osmotic resistance of Norway spruce (Picea abies) ETs during cryoprotectant pretreatment. Thus, RNA-seq, histological, and analytical chemical analyses are performed on the stable transformations of PaMYB11-OE and PaMYB11-SRDX in Norway spruce ETs. DAP-seq, Y1H, and LUC are further combined to explore the PaMYB11 targets. Activation of PaMYB11 is necessary and sufficient for suberin lamellae deposition on Norway spruce embryogenic cell walls, which plays a decisive role in ET survival under osmotic stress. Transcriptome analysis shows that PaMYB11 enhances suberin lamellae monomer synthesis by promoting very long-chain fatty acid (VLCFA) synthesis. PaPOP, PaADH1, and PaTET8L, the first two (PaADH1 and PaPOP, included) involved in VLCFA synthesis, are proved to be the direct targets of PaMYB11. Our study identified a novel osmotic response directed by PaMYB11 in Norway spruce ET, which provides a new understanding of the resistance mechanism against osmosis in gymnosperms.
Assuntos
Criopreservação , Lipídeos , Pressão Osmótica , Picea , Proteínas de Plantas , Picea/genética , Picea/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Criopreservação/métodos , Osmose , Regulação da Expressão Gênica de Plantas , Fatores de Transcrição/metabolismo , Fatores de Transcrição/genética , Parede Celular/metabolismoRESUMO
Climate change is raising concerns about how forests will respond to extreme droughts, heat waves and their co-occurrence. In this greenhouse study, we tested how carbon and water relations relate to seedling growth and mortality of northeastern US trees during and after extreme drought, warming, and combined drought and warming. We compared the response of our focal species red spruce (Picea rubens Sarg.) with a common associate (paper birch, Betula papyrifera Marsh.) and a species expected to increase abundance in this region with climate change (northern red oak, Quercus rubra L.). We tracked growth and mortality, photosynthesis and water use of 216 seedlings of these species through a treatment and a recovery year. Each red spruce seedling was planted in containers either alone or with another seedling to simulate potential competition, and the seedlings were exposed to combinations of drought (irrigated, 15-d 'short' or 30-d 'long') and temperature (ambient or 16 days at +3.5 °C daily maximum) treatments. We found dominant effects of the drought reducing photosynthesis, midday water potential, and growth of spruce and birch, but that oak showed considerable resistance to drought stress. The effects of planting seedlings together were moderate and likely due to competition for limited water. Despite high temperatures reducing photosynthesis for all species, the warming imposed in this study minorly impacted growth only for oak in the recovery year. Overall, we found that the diverse water-use strategies employed by the species in our study related to their growth and recovery following drought stress. This study provides physiological evidence to support the prediction that native species to this region like red spruce and paper birch are susceptible to future climate extremes that may favor other species like northern red oak, leading to potential impacts on tree community dynamics under climate change.
Assuntos
Betula , Mudança Climática , Secas , Picea , Quercus , Árvores , Quercus/crescimento & desenvolvimento , Quercus/fisiologia , Picea/crescimento & desenvolvimento , Picea/fisiologia , Betula/crescimento & desenvolvimento , Betula/fisiologia , Árvores/crescimento & desenvolvimento , Árvores/fisiologia , Plântula/crescimento & desenvolvimento , Plântula/fisiologia , Fotossíntese/fisiologia , New England , Água/metabolismo , Resistência à SecaRESUMO
Climate warming poses major threats to temperate forests, but the response of tree root metabolism has largely remained unclear. We examined the impact of long-term soil warming (>14 years, +4°C) on the fine root metabolome across three seasons for 2 years in an old spruce forest, using a liquid chromatography-mass spectrometry platform for primary metabolite analysis. A total of 44 primary metabolites were identified in roots (19 amino acids, 12 organic acids and 13 sugars). Warming increased the concentration of total amino acids and of total sugars by 15% and 21%, respectively, but not organic acids. We found that soil warming and sampling date, along with their interaction, directly influenced the primary metabolite profiles. Specifically, in warming plots, concentrations of arginine, glycine, lysine, threonine, tryptophan, mannose, ribose, fructose, glucose and oxaloacetic acid increased by 51.4%, 19.9%, 21.5%, 19.3%, 22.1%, 23.0%, 38.0%, 40.7%, 19.8% and 16.7%, respectively. Rather than being driven by single compounds, changes in metabolite profiles reflected a general up- or downregulation of most metabolic pathway network. This emphasises the importance of metabolomics approaches in investigating root metabolic pathways and understanding the effects of climate change on tree root metabolism.
Assuntos
Florestas , Picea , Raízes de Plantas , Solo , Picea/metabolismo , Raízes de Plantas/metabolismo , Solo/química , Mudança Climática , Metaboloma , Metabolômica , Árvores/metabolismo , Aminoácidos/metabolismoRESUMO
Due to integrated pollution prevention and control measures and the reduced burning of coal, air concentrations of mercury (Hg0) are currently levelling off. In the future, however, evasion from land surfaces will probably reverse this trend. Reasons are the rising temperatures and the loss of forest cover caused by calamities, droughts, storms and wildfires. Plant leaves constitute an important matrix for the accumulation of gaseous mercury and uptake and re-volatilisation by plants depends on the species, the vitality and the age and morphology of leaf organs. It has been shown that older conifer needles show higher concentrations than young needles and Hg accumulation is increasing throughout the season. In present study, we collected branches from Norway Spruce (Picea abies) in a former cinnabar mining region in Northern Palatinate, where artisanal and small-scale mining left innumerable waste dumps. While mining, smelting and processing of the ores were terminated during WWII, high total mercury concentrations remained in the top soils locally, with presumably only small fractions being plant available. In the lab, up to seven needle age classes were analysed. 1000 needle weights increased with age and as expected, also the Hg concentrations were elevated in the older needles. Needle concentrations were higher than those reported from other national biomonitoring programs confirming the regional imprint from legacy mercury. To complement our biomonitoring study, we collected edible mushrooms in former mining areas. Hg concentrations in most samples exceeded the EU maximum residue levels (MRL), while only a few broke the existing cadmium and lead limits. Tolerable weekly intake (TWI) for inorganic mercury would be surpassed with the consumption of a small portion of mushrooms. Further studies should be performed on the outgassing of Hg from mine wastes and the incorporation of Hg in the local food web, including its methylation and biomagnification.
Assuntos
Monitoramento Ambiental , Mercúrio , Mineração , Folhas de Planta , Poluentes do Solo , Mercúrio/análise , Mercúrio/metabolismo , Monitoramento Ambiental/métodos , Folhas de Planta/química , Alemanha , Poluentes do Solo/análise , Agaricales/química , Picea/química , Traqueófitas/química , Compostos de MercúrioRESUMO
Picea schrenkiana is the dominant tree species in Ili River Basin located in the western Tianshan Mountains of Xinjiang. We investigated the growth decline characteristics of P. schrenkiana at different altitudes (1800, 2300 and 2800 m) based on tree-ring index (TRI) and percentage growth change (GC), aiming to understand the growth response of P. schrenkiana to drought events at different altitudes and the impacts of altitude on tree growth decline in this region. The results showed that P. schrenkiana experienced multiple decline events at low-altitude (1800 m). TRI and GC identified inconsistent occurrence time of the decline events. The variations of TRI indicated that P. schrenkiana at low-altitude experienced two large-scale declines during 1927-1933 and 2017-2014, respectively. The variations of GC identified four decline events, including 1891-1893, 1924-1926, 1973-1975, and 2004-2009. The radial growth of P. schrenkiana across altitudes from low to high was significantly affected by the Palmer drought severity index (PDSI) of the previous growing season. The impact of current PDSI on P. schrenkiana during the growing season initially enhanced but later decreased with increasing altitude. In the extreme drought year 1917, the magnitude of growth decline increased with altitude. At low-altitude (1800 m), the TRI was 0.65, which was 35% lower than the normal level. At mid-altitude (2300 m) and high-altitude (2800 m), it was 0.56 and 0.54, respectively, being 40% lower than the average level. The drought event in 1917 had a 2-year legacy effect on the growth of P. schrenkiana at all the altitudes, with the TRI in 1920 recovered to exceeding 0.9, being close to the normal level. The impact of altitude on drought-induced forest decline was significant. Tree growth in low-altitude areas was more vulnerable to drought events due to the relatively poorer water and temperature conditions at low-altitude, which could lead to multiple large-scale decline events. In mid- and high-altitude areas, where hydrothermal conditions were more favorable, trees could experience even more severe decline during extreme droughts.
Assuntos
Altitude , Secas , Picea , China , Picea/crescimento & desenvolvimento , Ecossistema , RiosRESUMO
We conducted field surveys on foraging habitat and foraging activities of Picoides tridactylus in Liangshui National Nature Reserve of Heilongjiang Province, China, from April to May and November to December 2022. By using the resource selection function, we analyzed the factors affecting foraging habitat selection of P. tridactylus, compared the differences between foraging habitat selection and foraging activities in winter and spring by chi-square and Mann-Whitney U tests, and investigated their foraging preference with Bailey's method. The results showed that dominant tree species and dead arbor number were the important factors affecting foraging habitat selection of P. tridactylus. They preferred habitats with a large number of dead arbor and dominant trees, such as Picea asperata and Abies fabri. They preferred trees with a height of 10-20 m and a diameter at breast height of 15-45 cm. In spring, they favored semi-withered arbors and showed random utilization of P. koraiensis. During winter, they preferred dead arbors and avoided choosing P. koraiensis. They preferred to forage on tree trunk, in spring pecking in the middle of the tree for a short duration, and during winter, digging in the upper part of the tree for a long duration. Foraging habitat selection and foraging activities of P. koraiensis showed certain differences between winter and spring.
Assuntos
Ecossistema , Estações do Ano , China , Animais , Árvores/crescimento & desenvolvimento , Comportamento Alimentar , Picea/crescimento & desenvolvimento , Conservação dos Recursos NaturaisRESUMO
Our study delved into the relationship between root-associated fungi, gene expression and plant morphology in Norway spruce cuttings derived from both slow-and fast-growing trees. We found no clear link between the gene expression patterns of adventitious roots and the growth phenotype, suggesting no fundamental differences in the receptiveness to fungal symbionts between the phenotypes. Interestingly, saplings from slow-growing parental trees exhibited a higher richness of ectomycorrhizal species and larger roots. Some ectomycorrhizal species, typically found on mature spruces, were more prevalent on saplings from slow-growing spruces. The ericoid mycorrhizal fungus, Hyaloscypha hepaticola, showed a stronger association with saplings from fast-growing spruces. Moreover, saplings from slow-growing spruces had a greater number of Ascomycete taxa and free-living saprotrophic fungi. Aboveground sapling stems displayed some phenotypic variation; saplings from fast-growing phenotypes had longer branches but fewer whorls in their stems compared to those from the slow-growing group. In conclusion, the observed root-associated fungi and phenotypic characteristics in young Norway spruces may play a role in their long-term growth rate. This suggests that the early interactions between spruces and fungi could potentially influence their growth trajectory.
Assuntos
Micorrizas , Picea , Raízes de Plantas , Picea/microbiologia , Picea/crescimento & desenvolvimento , Raízes de Plantas/microbiologia , Raízes de Plantas/crescimento & desenvolvimento , Micorrizas/genética , Micorrizas/crescimento & desenvolvimento , Micorrizas/fisiologia , Noruega , Simbiose , Fungos/genética , Fungos/classificação , Fungos/crescimento & desenvolvimento , Ascomicetos/genética , Ascomicetos/crescimento & desenvolvimentoRESUMO
BACKGROUND: Forest geneticists typically use provenances to account for population differences in their improvement schemes; however, the historical records of the imported materials might not be very precise or well-aligned with the genetic clusters derived from advanced molecular techniques. The main objective of this study was to assess the impact of marker-based population structure on genetic parameter estimates related to growth and wood properties and their trade-offs in Norway spruce, by either incorporating it as a fixed effect (model-A) or excluding it entirely from the analysis (model-B). RESULTS: Our results indicate that models incorporating population structure significantly reduce estimates of additive genetic variance, resulting in substantial reduction of narrow-sense heritability. However, these models considerably improve prediction accuracies. This was particularly significant for growth and solid-wood properties, which showed to have the highest population genetic differentiation (QST) among the studied traits. Additionally, although the pattern of correlations remained similar across the models, their magnitude was slightly lower for models that included population structure as a fixed effect. This suggests that selection, consistently performed within populations, might be less affected by unfavourable genetic correlations compared to mass selection conducted without pedigree restrictions. CONCLUSION: We conclude that the results of models properly accounting for population structure are more accurate and less biased compared to those neglecting this effect. This might have practical implications for breeders and forest managers where, decisions based on imprecise selections can pose a high risk to economic efficiency.