RESUMO
Serum pepsinogen A (PGA) and total pepsin activity (PA) in gastric juice were investigated in 87 duodenal ulcer patients. The analysis of data shows a significant increase of PGA and PA in comparison with controls. The grouping of duodenal ulcer patients according to Helicobacter Pylori (HP) infection revealed in HP positive patients a significant increase of PGA, while PA does not change. This contrast may be due to various factors: in particular the two parameters are an expression of different cellular activities and the PA in gastric juice is expression of all pepsinogens. It can be concluded that the damaging action of HP on duodenal mucosa is due to a direct action and not related to alteration of pepsin activity in gastric juice. Moreover, serum PGA can be held as useful parameter of HP infection.
Assuntos
Úlcera Duodenal/metabolismo , Infecções por Helicobacter/metabolismo , Helicobacter pylori , Pepsina A/análise , Pepsinogênios/análise , Adulto , Úlcera Duodenal/microbiologia , Feminino , Suco Gástrico/química , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
The immunohistochemical expression of pepsinogen I (PI) and pepsinogen II (PII) was studied in 103 gallbladder carcinomas, 25 non tumoral gallbladder lesions and 23 gallbladder cancer metastases. PI was positive in 6% of carcinomas and only in 2% of non tumoral contiguous mucosa. PII was positive in 46% of carcinomas and 43% of controls. Metastases were negative for both enzymes. Pyloric gland metaplasia of non tumoral mucosa adjacent to the tumor expressed PII in 68.9% and PI in 3.4% of cases. The same lesion in non tumoral gallbladders was positive for PII in 66.6% of cases, and no case expressed PI. There was no relationship between PI expression and the histological degree of differentiation. Only tumors with serosal or subserosal involvement were positive for PI. PII had a higher expression in early or well differentiated tumors. Tumors with pyloric gland metaplasia in the contiguous non tumoral mucosa expressed PII more frequently. Our results, based on the expression of PI and PII, suggest a relationship between the presence of pyloric gland metaplasia and some types of gallbladder cancer.
Assuntos
Biomarcadores Tumorais/análise , Carcinoma/enzimologia , Neoplasias da Vesícula Biliar/enzimologia , Vesícula Biliar/enzimologia , Isoenzimas/análise , Metástase Neoplásica , Proteínas de Neoplasias/análise , Pepsinogênios/análise , Carcinoma/patologia , Diferenciação Celular , Vesícula Biliar/patologia , Doenças da Vesícula Biliar/enzimologia , Doenças da Vesícula Biliar/patologia , Neoplasias da Vesícula Biliar/patologia , Mucosa Gástrica/enzimologia , Humanos , Hiperplasia , Técnicas Imunoenzimáticas , Metaplasia , Invasividade Neoplásica , Lesões Pré-Cancerosas/enzimologia , Lesões Pré-Cancerosas/patologia , PiloroRESUMO
Gastric secretion was checked in two groups of rats with pyloric ligature and experimental hypovolemia subjecting one of the groups to acute hypoxia; in this group there was significant decrease in volume, acid and pepsin secretion and potassium concentration in gastric contents. In gastric mucosa pepsinogen was increased. We wish to emphasise in these changes, the importance of water and hypoxia in electrolytic balance.
Assuntos
Volume Sanguíneo , Suco Gástrico/metabolismo , Hipóxia/fisiopatologia , Animais , Cloretos/análise , Feminino , Suco Gástrico/análise , Mucosa Gástrica/análise , Masculino , Pepsina A/análise , Pepsinogênios/análise , Potássio/análise , Ratos , Sódio/análise , Equilíbrio HidroeletrolíticoRESUMO
Gastric secretion was studied in two groups of rats in which pyloric ligature was made according to Dai's method. Both groups were put in a hypopressure chamber. One of the groups was normovolemic. The other group was made hypovolemic by acute bleeding. The following parameters were checked in gastire contents: volumen, acid and pepsin secretion, U.P./g. of stomach, chloride, potassium and sodium; in gastric mucosa total U.P. and pepsinogen concentration were measured. In the rats subjected to acute hypovolemia and hypoxia it was found a significant decrease in acid secretion, chloride and potassium mEq/1; there were no statistically significant changes in volume, pepsin secretion; U.P./g. of stomach and sodium mEq/1. Total U. P. and U.P./. of stomach increased in the gastirc mucosa.