RESUMO
Abstract Pediococcus acidilactici strain S1, a lactic acid-fermenting bacterium, was isolated from makgeolli-a Korean traditional fermented alcoholic beverage. Here we report the 1,980,172 bp (G + C content, 42%) genome sequence of Pediococcus acidilactici strain S1 with 1,525 protein-coding sequences (CDS), of which 47% could be assigned to recognized functional genes. The genome sequence of the strain S1 might provide insights into the genetic basis of the lactic acid bacterium with alcohol-tolerant.
Assuntos
Genoma Bacteriano , Ácido Láctico/metabolismo , Bebidas Alcoólicas/microbiologia , Pediococcus acidilactici/isolamento & purificação , Pediococcus acidilactici/genética , Sequência de Bases , República da Coreia , Fermentação , Pediococcus acidilactici/metabolismo , Sequenciamento Completo do GenomaRESUMO
Pediococcus acidilactici strain S1, a lactic acid-fermenting bacterium, was isolated from makgeolli-a Korean traditional fermented alcoholic beverage. Here we report the 1,980,172bp (G+C content, 42%) genome sequence of Pediococcus acidilactici strain S1 with 1,525 protein-coding sequences (CDS), of which 47% could be assigned to recognized functional genes. The genome sequence of the strain S1 might provide insights into the genetic basis of the lactic acid bacterium with alcohol-tolerant.
Assuntos
Bebidas Alcoólicas/microbiologia , Genoma Bacteriano , Ácido Láctico/metabolismo , Pediococcus acidilactici/genética , Pediococcus acidilactici/isolamento & purificação , Sequência de Bases , Fermentação , Pediococcus acidilactici/metabolismo , República da Coreia , Sequenciamento Completo do GenomaRESUMO
Abstract Pediococcus acidilactici strain K3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional Korean fermentation starter for makgeolli brewing. Draft genome of this strain was approximately 1,991,399 bp (G+C content, 42.1%) with 1525 protein-coding sequences (CDS), of which 44% were assigned to recognized functional genes. This draft genome sequence data of the strain K3 will provide insights into the genetic basis of its alcohol-tolerance.
Assuntos
Adaptação Biológica/efeitos dos fármacos , Adaptação Biológica/genética , Genoma Bacteriano , Etanol/farmacologia , Pediococcus acidilactici/efeitos dos fármacos , Pediococcus acidilactici/genética , Ácido Láctico/biossíntese , Biologia Computacional/métodos , Genômica/métodos , Etanol/metabolismo , Fermentação , Sequenciamento de Nucleotídeos em Larga Escala , Anotação de Sequência Molecular , Pediococcus acidilactici/isolamento & purificação , Pediococcus acidilactici/metabolismoRESUMO
Pediococcus acidilactici strain K3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional Korean fermentation starter for makgeolli brewing. Draft genome of this strain was approximately 1,991,399bp (G+C content, 42.1%) with 1525 protein-coding sequences (CDS), of which 44% were assigned to recognized functional genes. This draft genome sequence data of the strain K3 will provide insights into the genetic basis of its alcohol-tolerance.
Assuntos
Adaptação Biológica/efeitos dos fármacos , Adaptação Biológica/genética , Etanol/farmacologia , Genoma Bacteriano , Pediococcus acidilactici/efeitos dos fármacos , Pediococcus acidilactici/genética , Biologia Computacional/métodos , Etanol/metabolismo , Fermentação , Genômica/métodos , Sequenciamento de Nucleotídeos em Larga Escala , Ácido Láctico/biossíntese , Anotação de Sequência Molecular , Pediococcus acidilactici/isolamento & purificação , Pediococcus acidilactici/metabolismoRESUMO
During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01 and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance, after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 but inactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1-9% NaCl concentration. The plate overlay assay showed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P. acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1).