RESUMO
The genus Ochrobactrum belongs to the family Brucellaceae and its members are known to be adapted to a wide range of ecological niches. Ochrobactrum anthropi ATCC 49188T and Ochrobactrum lupini LUP21T are strains isolated from human clinical and plant root nodule samples, respectively, which share high similarity for phylogenetic markers (i.e 100â% for 16S rRNA, 99.9â% for dnaK and 99.35â% for rpoB). In this work, multiple genome average nucleotide identity (ANI) approaches, digital DNA-DNA hybridization (dDDH) and phylogenetic analysis were performed in order to investigate the taxonomic relationship between O. anthropi ATCC 49188T, O. lupini LUP21T, and other five type strains from the genus Ochrobactrum. Whole-genome comparisons demonstrated that O. lupini LUP21T and the Ochrobactrum genus type species, O. anthropi ATCC 49188T, share 97.55â% of ANIb, 98.25â% of ANIm, 97.99â% of gANI, 97.94â% of OrthoANI and 83.9â% of dDDH, which exceed the species delineation thresholds. These strains are also closely related in phylogenies reconstructed from a concatenation of 1193 sequences from single-copy ortholog genes. A review of their profiles revealed that O. anthropi ATCC 49188T and O. lupini LUP21T do not present pronounced differences at phenotypic and chemotaxonomic levels. Considering phylogenetic, genomic, phenotypic and chemotaxonomic data, O. lupini should be considered a later heterotypic synonym of O. anthropi.
Assuntos
Ochrobactrum anthropi/classificação , Ochrobactrum/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Humanos , Hibridização de Ácido Nucleico , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
Introducción: El semen es una mezcla compleja de fluidos y células que posee las condiciones adecuadas para albergar microorganismos, especialmente bacterias. Objetivo: Evaluar la presencia de bacterias en el semen de individuos normozoospérmicos asintomáticos para infecciones urogenitales. Métodos: Se realizó una secuenciación estándar posterior a la amplificación por PCR con el uso de los cebadores universales 27F y 1492R para identificación de bacterias, en 10 muestras de semen de voluntarios normozoospérmicos asintomáticos para infecciones urogenitales. Resultados: Se identificó a Ochrobactrum anthropi en 8 de las 10 muestras seminales evaluadas y a Haemophilus paraurethrae o Escherichia coli en los dos restantes. O. anthropi es una bacteria comensal, ampliamente distribuida en la naturaleza, especialmente en las fuentes de agua que, a pesar de su baja virulencia, ocasionalmente causa infecciones en individuos inmunocomprometidos. Conclusión: La alta frecuencia de O. anthropi en las muestras de semen de individuos normozoospérmicos asintomáticos para infecciones urogenitales puede asociarse a procesos de contaminación durante la recolección de la muestra, debido a la amplia distribución de esta bacteria, especialmente en las fuentes de agua(AU)
Introduction: Semen is a complex combination of fluids and cells that can harbor microorganisms, especially bacteria. Objective: To assess the presence of bacteria in semen samples from asymptomatic normozoospermic individuals, for urogenital infections. Methods: Standard sequencing after PCR amplification was performed with the use of the universal primers 27F and 1492R for bacterial identification, in 10 semen samples of asymptomatic normozoospermic volunteers for urogenital infections. Results: Thisidentified Ochrobactrum anthropi in 8 out of 10 samples assessed. In the remaining two samples, we identified Haemophilus paraurethrae and Escherichia coli. O. anthropi is a commensal bacterium, widely spread in nature, especially in water sources that, despite its low virulence, occasionally cause infections in immune compromised individuals. Conclusion: The high frequency of O. anthropi in semen samples from asymptomatic normozoospermic individuals, for urogenital infections can be associated with contamination during the collection of the sample, due to the wide distribution of this bacterium, especially in water sources(AU)
Assuntos
Humanos , Masculino , Adulto , Sêmen/microbiologia , Infecções Bacterianas/microbiologia , Reação em Cadeia da Polimerase/métodos , Ochrobactrum anthropi/isolamento & purificação , Poluição da Água/efeitos adversosRESUMO
Ochrobactrum and Brucella are genetically related genera of the family Brucellaceae, sharing 98.8% rRNA similarity. Because of their phenotypic similarity, Ochrobactrum can be miscoded as Brucella by automated identification systems. The misidentification on blood cultures (BCs) of B. suis as O. anthropi by the VITEK 2 system is herein described. A 67-year-old male with a prosthetic mitral valve and fever was admitted with bacteremia due to a Gram-negative coccobacillus identified as O. anthropi by VITEK 2. The patient's fever persisted along with positive blood cultures despite specific antimicrobial treatment. Due to this adverse outcome, the patient was interrogated again and admitted having domestic swine. Serological tests were positive for acute brucellosis. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of BC strains identified B. suis biovar 1. Timely identification of Brucella is essential for providing proper treatment to the patient and for advising safe handling of laboratory cultures in biological safety cabinets to prevent laboratory-acquired infection. Countries where brucellosis is endemic must be aware of this possibility.
Assuntos
Bacteriemia/diagnóstico , Brucella suis/classificação , Brucella suis/isolamento & purificação , Brucelose/diagnóstico , Erros de Diagnóstico , Ochrobactrum anthropi/classificação , Idoso , Animais , Automação Laboratorial , Técnicas de Tipagem Bacteriana , Doenças Transmitidas por Alimentos/diagnóstico , Genótipo , Humanos , Masculino , Fenótipo , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Testes Sorológicos , SuínosRESUMO
Ochrobactrum spp is a gram-negative bacillus currently considered an emerging and opportunistic infection, rare inhumans, and generally associated with indwelling foreign bodies. We report a case of primary bloodstream infectionrelated to a dialysis catheter, caused by Ochrobactrum oryzae misidentified...
Assuntos
Diálise , Ochrobactrum anthropi , SangueRESUMO
Fluoranthene and pyrene are polycyclic aromatic hydrocarbons of high molecular weight that are recalcitrant and toxic to humans; therefore, their removal from the environment is crucial. From hydrocarbon-contaminated soil, 25 bacteria and 12 filamentous fungi capable of growth on pyrene and fluoranthene as the sole carbon and energy source were isolated. From these isolates, Ochrobactrum anthropi BPyF3 and Fusarium sp. FPyF1 were selected and identified because they grew quickly and abundantly in both hydrocarbons. Furthermore, O. anthropi BPyF3 and Fusarium sp. FPyF1 were most efficient at removing pyrene (50.39 and 51.32 %, respectively) and fluoranthene (49.85 and 49.36 %, respectively) from an initial concentration of 50 mg L(-1) after 7 days of incubation. Based on this and on the fact that there was no antagonism between the two microorganisms, a coculture composed of O. anthropi BPyF3 and Fusarium sp. FPyF1 was formed to remove fluoranthene and pyrene at an initial concentration of 100 mg L(-1) in a removal kinetic assay during 21 days. Fluoranthene removal by the coculture was higher (87.95 %) compared with removal from the individual cultures (68.95 % for Fusarium sp. FPyF1 and 64.59 % for O. anthropi BPyF3). In contrast, pyrene removal by the coculture (99.68 %) was similar to that obtained by the pure culture of Fusarium sp. FPyF1 (99.75 %). The kinetics of removal for both compounds was adjusted to a first-order model. This work demonstrates that the coculture formed by Fusarium sp. FPyF1 and O. anthropi BPyF3 has greater potential to remove fluoranthene than individual cultures; however, pyrene can be removed efficiently by Fusarium sp. FPyF1 alone.
Assuntos
Técnicas de Cocultura , Fluorenos/isolamento & purificação , Fluorenos/metabolismo , Fusarium/metabolismo , Ochrobactrum anthropi/metabolismo , Pirenos/isolamento & purificação , Pirenos/metabolismo , Biodegradação Ambiental , Meios de Cultura/química , Poluentes Ambientais/isolamento & purificação , Poluentes Ambientais/metabolismo , Fusarium/crescimento & desenvolvimento , Cinética , Ochrobactrum anthropi/crescimento & desenvolvimentoRESUMO
Ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. We report the clinical and microbiological characteristics of Ochrobactrum anthropi bacteremia in a preterm patient.
Assuntos
Bacteriemia/diagnóstico , Bacteriemia/patologia , Fibrose Cística/complicações , Ochrobactrum anthropi/isolamento & purificação , Adulto , Antibacterianos/farmacologia , Bacteriemia/microbiologia , Feminino , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Testes de Sensibilidade Microbiana , Ochrobactrum anthropi/classificação , Ochrobactrum anthropi/efeitos dos fármacos , Ochrobactrum anthropi/genéticaRESUMO
Ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. We report the clinical and microbiological characteristics of Ochrobactrum anthropi bacteremia in a preterm patient.
Assuntos
Adulto , Feminino , Humanos , Recém-Nascido , Bacteriemia/diagnóstico , Bacteriemia/patologia , Fibrose Cística/complicações , Ochrobactrum anthropi/isolamento & purificação , Antibacterianos/farmacologia , Bacteriemia/microbiologia , Recém-Nascido Prematuro , Testes de Sensibilidade Microbiana , Ochrobactrum anthropi/classificação , Ochrobactrum anthropi/efeitos dos fármacos , Ochrobactrum anthropi/genéticaRESUMO
Ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. We report the clinical and microbiological characteristics of Ochrobactrum anthropi bacteremia in a preterm patient.
Assuntos
Humanos , Feminino , Recém-Nascido , Adulto , Bacteriemia/diagnóstico , Bacteriemia/patologia , Fibrose Cística/complicações , Ochrobactrum anthropi/isolamento & purificação , Antibacterianos/farmacologia , Bacteriemia/microbiologia , Recém-Nascido Prematuro , Testes de Sensibilidade Microbiana , Ochrobactrum anthropi/classificação , Ochrobactrum anthropi/efeitos dos fármacos , Ochrobactrum anthropi/genéticaRESUMO
Postoperative endophthalmitis often progresses to significant visual impairment. This paper describes an outbreak of Ochrobactrum anthropi endophthalmitis following cataract surgery, and propose a new sterilization protocol to minimize the risk of further cases. Medical records of patients with O. anthropi endophthalmitis or with suggestive clinical findings during the outbreak were reviewed. Seven cases of O. anthropi pseudophakic endophthalmitis were confirmed between 24 July and 10 November 2010. The most probable cause of the outbreak of Ochrobactrum anthropi endophthalmitis was contamination of the tubing of the phaco-emulsification machine. Following introduction of a new sterilization protocol, no further cases occurred in over 1000 subsequent procedures.
Assuntos
Extração de Catarata/efeitos adversos , Surtos de Doenças , Endoftalmite/epidemiologia , Infecções por Bactérias Gram-Negativas/epidemiologia , Ochrobactrum anthropi/isolamento & purificação , Endoftalmite/microbiologia , Equipamentos e Provisões/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Humanos , Controle de Infecções/métodosRESUMO
Ochrobactrum anthropi is a rare pathogen in humans that is usually related to infections in inmunocompromised patients or people with indwelling medical devices. There are very few reported cases of O. anthropi bacteremia in patients on hemodialysis. We report a 19-year-old female on hemodialysis through a jugular catheter, with fever and malaise lasting one month. The catheter was withdrawn and Ochrobactrum anthropi was isolated from it and from blood cultures. The patient was successfully treated with levofloxacin.
Assuntos
Feminino , Humanos , Adulto Jovem , Bacteriemia/microbiologia , Infecções Relacionadas a Cateter/microbiologia , Cateteres de Demora/efeitos adversos , Infecções por Bactérias Gram-Negativas/microbiologia , Ochrobactrum anthropi/isolamento & purificação , Diálise Renal/efeitos adversosRESUMO
Ochrobactrum anthropi is a rare pathogen in humans that is usually related to infections in immunocompromised patients or people with indwelling medical devices. There are very few reported cases of O. anthropi bacteremia in patients on hemodialysis. We report a 19-year-old female on hemodialysis through a jugular catheter, with fever and malaise lasting one month. The catheter was withdrawn and Ochrobactrum anthropi was isolated from it and from blood cultures. The patient was successfully treated with levofloxacin.
Assuntos
Bacteriemia/microbiologia , Infecções Relacionadas a Cateter/microbiologia , Cateteres de Demora/efeitos adversos , Infecções por Bactérias Gram-Negativas/microbiologia , Ochrobactrum anthropi/isolamento & purificação , Diálise Renal/efeitos adversos , Feminino , Humanos , Adulto JovemRESUMO
Ochrobactrum anthtropi es un bacilo gram negativo aeróbico, no fermentador de la glucosa, anteriormente conocido como Achromobacter sp o CDC grupo Vd. Ha sido aislado del medio ambiente y de infecciones en seres humanos que generalmente presentaban algún tipo de inmunocompromiso. Las infecciones por este microorganismo fueron bacteriemias relacionadas a catéteres y en ocasiones endoftalmitis, infecciones urinarias, meningitis, endocarditis, absceso hepático, osteocondritis, absceso pelviano y absceso pancreático. Se presenta el caso de un paciente de sexo masculino, de 69 años de edad, que consultó a la guardia por hipotensión sostenida y síndrome febril de cuatro días de evolución, escalofrío, sudoración profusa y deterioro del sensorio. El paciente tenía diabetes de tipo 2 y antecedente de accidente cerebrovascular. Debido a insuficiencia renal crónica presentaba un catéter de doble lumen para la diálisis. Se documentó una bacteriemia relacionada a catéter por cultivo de sangre a través de catéter y de vena periférica, utilizando el sistema automatizado de hemocultivos Bact-Alert y la metodología de tiempo diferencial (>120min). La confirmación se realizó, una vez removido el catéter, por la técnica semicuantitativa de Maki (> 15 UFC). El microorganismo fue identificado por API 20NE y Vitek 1 como Ochrobactrum anthropi.
Ochrobactrum anthropi is a non-glucose fermentative, aerobic gram-negative bacillus, formerly known as Achromobacter sp or CDC group Vd. It has been isolated from the environment and from infections in usually immunocompromised human beings. The documented infections frequently involved catheter related bacteremia whereas endophthalmitis, urinary infections, meningitis, endocarditis, hepatic abscess, osteochondritis, pelvic abscess and pancreatic abscess were rarely involved. Here it is presented the case of a male patient aged 69 years with sustained hypotension, four day febrile syndrome, chill, lavish perspiration and sensorium deterioration. He had type 2 diabetes and antecedent of cerebrovascular accident. A double-lumen dialysis catheter was present due to chronic renal insufficiency. An episode of catheter-related bloodstream infection was documented by using Bact-Alert Blood Culture System and Differential-Time-to-Positivity Method for central venous catheter versus peripheral blood cultures (>120 min). Once removed, it was confirmed through Maki semi quantitative technique (>15 FCU). The microorganism was identified by API 20NE and Vitek 1 as Ochrobactrum anthropi.
Assuntos
Idoso , Humanos , Masculino , Bacteriemia/microbiologia , Infecções Relacionadas a Cateter/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Ochrobactrum anthropi/isolamento & purificação , Infecções Relacionadas a Cateter/diagnóstico , Cateteres de Demora/microbiologiaRESUMO
Reporte de caso de bacteriemia por Ochrobactrum anthropi con probable hepatitis bacteriana asociada, en un paciente inmunocompetente, el cual ingresa por sospecha de colangitis y obstrucción biliar. O. anthropi es una bacteria emergente en infecciones intrahospitalarias con notable resistencia antimicrobiana, y es un patógeno inusual en humanos.
A case report of bacteraemia by Ochrobactrum anthropi probably associated with bacterial hepatitis, in a inmuno competent patient, who was admitted to the hospital with the diagnostic impression of cholangitis and biliary obstruction. O. anthropi is an emerging bacteria in nosocomial infections with remarkable antimicrobial resistance, being an unusual pathogen in humans.
Assuntos
Bacteriemia , Colestase , Ochrobactrum anthropi , Infecção HospitalarRESUMO
Ochrobactrum anthropi is a non-glucose fermentative, aerobic gram-negative bacillus, formerly known as Achromobacter sp or CDC group Vd. It has been isolated from the environment and from infections in usually immunocompromised human beings. The documented infections frequently involved catheter related bacteremia whereas endophthalmitis, urinary infections, meningitis, endocarditis, hepatic abscess, osteochondritis, pelvic abscess and pancreatic abscess were rarely involved. Here it is presented the case of a male patient aged 69 years with sustained hypotension, four day febrile syndrome, chill, lavish perspiration and sensorium deterioration. He had type 2 diabetes and antecedent of cerebrovascular accident. A double-lumen dialysis catheter was present due to chronic renal insufficiency. An episode of catheter-related bloodstream infection was documented by using Bact-Alert Blood Culture System and Differential-Time-to-Positivity Method for central venous catheter versus peripheral blood cultures (>120 min). Once removed, it was confirmed through Maki semi quantitative technique (>15 FCU). The microorganism was identified by API 20NE and Vitek 1 as Ochrobactrum anthropi.
Assuntos
Bacteriemia/microbiologia , Infecções Relacionadas a Cateter/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Ochrobactrum anthropi/isolamento & purificação , Idoso , Infecções Relacionadas a Cateter/diagnóstico , Cateteres de Demora/microbiologia , Humanos , MasculinoRESUMO
Previous findings indicate that Brucella antigens and those from nonpathogenic alphaproteobacteria (NPAP) are cross-recognized by the immune system. We hypothesized that immunization with NPAP would protect mice from Brucella infection. Mice were immunized subcutaneously with heat-killed Ochrobactrum anthropi, Sinorhizobium meliloti, Mesorhizobium loti, Agrobacterium tumefaciens, or Brucella melitensis H38 (standard positive control) before intravenous challenge with Brucella abortus 2308. Cross-reacting serum antibodies against Brucella antigens were detected at the moment of challenge in all NPAP-immunized mice. Thirty days after B. abortus challenge, splenic CFU counts were significantly lower in mice immunized with O. anthropi, M. loti, and B. melitensis H38 than in the phosphate-buffered saline controls (protection levels were 0.80, 0.66, and 1.99 log units, respectively). In mice immunized intraperitoneally with cytosoluble extracts from NPAP or Brucella abortus, protection levels were 1.58 for the latter, 0.63 for O. anthropi, and 0.40 for M. loti. To test whether the use of live NPAP would increase protection further, mice were both immunized and challenged by the oral route. Immunization with NPAP induced a significant increase in serum immunoglobulin G (IgG), but not serum or fecal IgA, against Brucella antigens. After challenge, anti-Brucella IgA increased significantly in the sera and feces of mice orally immunized with O. anthropi. For all NPAP, protection levels were higher than those obtained with systemic immunizations but were lower than those obtained by oral immunization with heat-killed B. abortus. These results show that immunization with NPAP, especially O. anthropi, confers partial protection against Brucella challenge. However, such protection is lower than that conferred by immunization with whole Brucella or its cytosoluble fraction.