RESUMO
Physical exercise induces inflammatory and oxidative markers production in the skeletal muscle and this process is under the control of both endogenous and exogenous modulators. Recently, molecular hydrogen (H2) has been described as a therapeutic gas able to reduced oxidative stress in a number of conditions. However, nothing is known about its putative role in the inflammatory and oxidative status during a session of acute physical exercise in sedentary rats. Therefore, we tested the hypothesis that H2 attenuates both inflammation and oxidative stress induced by acute physical exercise. Rats ran at 80% of their maximum running velocity on a closed treadmill inhaling either the H2 gas (2% H2, 21% O2, balanced with N2) or the control gas (0% H2, 21% O2, balanced with N2) and were euthanized immediately or 3â¯h after exercise. We assessed plasma levels of inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1ß and IL-6] and oxidative markers [superoxide dismutase (SOD), thiobarbituric acid reactive species (TBARS) and nitrite/nitrate (NOx)]. In addition, we evaluated the phosphorylation status of intracellular signaling proteins [glycogen synthase kinase type 3 (GSK3α/ß) and the cAMP responsive element binding protein (CREB)] that modulate several processes in the skeletal muscle during exercise, including changes in exercise-induced reactive oxygen species (ROS) production. As expected, physical exercise increased virtually all the analyzed parameters. In the running rats, H2 blunted exercise-induced plasma inflammatory cytokines (TNF-α and IL-6) surges. Regarding the oxidative stress markers, H2 caused further increases in exercise-induced SOD activity and attenuated the exercise-induced increases in TBARS 3â¯h after exercise. Moreover, GSK3α/ß phosphorylation was not affected by exercise or H2 inhalation. Otherwise, exercise caused an increased CREB phosphorylation which was attenuated by H2. These data are consistent with the notion that H2 plays a key role in decreasing exercise-induced inflammation, oxidative stress, and cellular stress.
Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Hidrogênio/farmacologia , Músculo Esquelético/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/antagonistas & inibidores , Administração por Inalação , Animais , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/sangue , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/genética , Glicogênio Sintase Quinase 3 beta/sangue , Glicogênio Sintase Quinase 3 beta/genética , Interleucina-1beta/antagonistas & inibidores , Interleucina-1beta/sangue , Interleucina-1beta/genética , Interleucina-6/antagonistas & inibidores , Interleucina-6/sangue , Interleucina-6/genética , Isoenzimas/sangue , Isoenzimas/genética , Masculino , Músculo Esquelético/metabolismo , Músculo Esquelético/fisiopatologia , Nitratos/antagonistas & inibidores , Nitratos/sangue , Nitritos/antagonistas & inibidores , Nitritos/sangue , Condicionamento Físico Animal/métodos , Esforço Físico/efeitos dos fármacos , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/sangue , Corrida , Superóxido Dismutase/sangue , Superóxido Dismutase/genética , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/genéticaRESUMO
Reported models of denitrification rates integrate in an unique parameter the pH-dependent inhibition by HNO2 and the pH effect on the bacterial metabolic activity; furthermore, they do not quantify separately the pH effect on the nitrate and on the nitrite reduction rates. The goal of this work was to quantify both effects on the kinetics of nitrate and nitrite reduction to improve the models predictive value. Assays were performed at a pH range of 6.5-9.0 in batch reactors at 37ºC with an activated sludge. At the studied pH range and at below the HNO2 inhibitory concentration (0.004 mg L-1), the maximum nitrate reduction rate diminished 23 percent and 50 percent by decreasing or increasing, respectively, one pH unit from 8.0. The maximum nitrite reduction at pH 8.0 diminished 15 percent at pH 7.0 and 40 percent at pH 9.0. At HNO2 concentrations over the inhibitory concentration, except at pH > 8.0, the maximum nitrate reduction rate diminished 50 percent upon decreasing the pH from 8.0 to 7.0 or increasing it from 8.0 to 9.0. Inclusion of the pH effect in the reported models improved their predictive value; average deviations from the experimental data were reduced from 53 percent to 10.7 percent or 33.8 percent to 10.5 percent for nitrite and nitrate reduction rates, respectively.
Assuntos
Desnitrificação , Concentração de Íons de Hidrogênio , Nitratos/antagonistas & inibidores , Fenômenos Químicos , Paracoccus denitrificans/enzimologia , Pseudomonas fluorescens/enzimologiaRESUMO
Plant-food-derived antioxidants and active principles such as flavonoids, hydroxycinnamates (ferulic acid, chlorogenic acids, vanillin etc.), beta-carotene and other carotenoids, vitamin E, vitamin C, or rosemary, sage, tea and numerous extracts are increasingly proposed as important dietary antioxidant factors. In this endeavor, assays involving oxidative DNA damage for characterizing the potential antioxidant actions are suggested as in vitro screens of antioxidant efficacy. The critical question is the bioavailability of the plant-derived antioxidants.
Assuntos
Antioxidantes , Dano ao DNA , Estresse Oxidativo , Plantas Comestíveis/química , Antioxidantes/análise , Antioxidantes/metabolismo , Cobre/metabolismo , Adutos de DNA/análise , Adutos de DNA/genética , Nitratos/antagonistas & inibidores , Nitratos/metabolismo , Fenantrolinas/metabolismo , Plantas Comestíveis/metabolismoRESUMO
Peroxynitrite (ONOO-) is a potent oxidizing and nitrating agent produced by the reaction of nitric oxide with superoxide. It readily nitrates phenolic compounds such as tyrosine residues in proteins, and it has been demonstrated that nitration of tyrosine residues in proteins inhibits their phosphorylation. During immune responses, tyrosine phosphorylation of key substrates by protein tyrosine kinases is the earliest of the intracellular signaling pathways following activation through the TCR complex. This work was aimed to evaluate the effects of ONOO- on lymphocyte tyrosine phosphorylation, proliferation, and survival. Additionally, we studied the generation of nitrating species in vivo and in vitro during immune activation. Our results demonstrate that ONOO-, through nitration of tyrosine residues, is able to inhibit activation-induced protein tyrosine phosphorylation in purified lymphocytes and prime them to undergo apoptotic cell death after PHA- or CD3-mediated activation but not upon phorbol ester-mediated stimulation. We also provide evidence indicating that peroxynitrite is produced during in vitro immune activation, mainly by cells of the monocyte/macrophage lineage. Furthermore, immunohistochemical studies demonstrate the in vivo generation of nitrating species in human lymph nodes undergoing mild to strong immune activation. Our results point to a physiological role for ONOO- as a down-modulator of immune responses and also as key mediator in cellular and tissue injury associated with chronic activation of the immune system.
Assuntos
Apoptose/efeitos dos fármacos , Imunossupressores/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Nitratos/farmacologia , Linfócitos T/imunologia , Tirosina/metabolismo , Apoptose/imunologia , Complexo CD3/fisiologia , Divisão Celular/efeitos dos fármacos , Divisão Celular/imunologia , Células Cultivadas , Relação Dose-Resposta Imunológica , Humanos , Linfonodos/imunologia , Linfonodos/patologia , Metaloporfirinas/farmacologia , Monócitos/fisiologia , Nitratos/antagonistas & inibidores , Nitratos/metabolismo , Óxido Nítrico Sintase/antagonistas & inibidores , Fosforilação/efeitos dos fármacos , Proteínas/metabolismo , Superóxido Dismutase/farmacologia , Linfócitos T/citologia , Linfócitos T/enzimologia , Linfócitos T/metabolismo , Fatores de Tempo , Tirosina/análogos & derivadosRESUMO
Rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was inactivated by peroxynitrite under biologically relevant conditions. The decrease of enzymatic activity followed an exponential function, and the concentration of peroxynitrite needed to inactivate 50% of 7 microM GAPDH (IC50) was 17 microM. Hydroxyl radical scavengers did not protect GAPDH from inactivation, but molecules that react directly with peroxynitrite such as cysteine, glutathione, or methionine and the substrate, glyceraldehyde 3-phosphate, afforded significant protection. Assuming simple competition kinetics between scavengers and the enzyme, we estimated a second-order rate constant of (2.5 +/- 0.5) x 10(5) M-1 s-1 at 25 degreesC and pH 7.4 for the GAPDH tetramer. The loss of enzyme activity was accompanied by protein thiol oxidation (two thiols oxidized per subunit) with only one critical thiol responsible of enzyme inactivation. Indeed, the pH profile of inactivation was consistent with the reaction of GAPDH sulfhydryls (GAPDH-SH) with peroxynitrite. Peroxynitrite-inactivated GAPDH was resistant to arsenite reduction and only 15% recovered by 20 mM dithiothreitol, suggesting that GAPDH-SH has been mainly oxidized to sulfinic or sulfonic acid, with a minor proportion yielding a disulfide. On the other hand, under anaerobic conditions the peroxynitrite precursor, nitric oxide (*NO), only slowly inactivated GAPDH with a rate constant of 11 M-1 s-1. The remarkable reactivity of the critical thiol group in GAPDH (Cys-149) toward peroxynitrite, which is one order of magnitude higher than that of previously studied sulfhydryls, indicate that it may constitute a preferential intracellular target for peroxynitrite.