RESUMO
BACKGROUND: The aims of this study were to investigate the immunolocalization of ezrin and its relationship with the podoplanin expression in keratocystic odontogenic tumors. MATERIAL AND METHODS: The immunohistochemical expressions of ezrin and podoplanin by odontogenic epithelium were evaluated in keratocystic odontogenic tumors using monoclonal antibodies. RESULTS: Our results showed strong cytoplasmic ezrin and membranous podoplanin expressions in basal epithelial layer of all keratocystic odontogenic tumors. The cytoplasmic and membranous ezrin expressions were also detected in suprabasal epithelial layers of tumors. Statistically significant difference between cellular immunolocalization of ezrin and podoplanin odontogenic epithelium were found by Wilcoxon's test (p < 0.05). No correlation between both proteins in keratocystic odontogenic tumors was detected by Spearman test. CONCLUSIONS: These results suggest that ezrin and podoplanin may contribute to the expansive growth and local invasiveness of keratocystic odontogenic tumors. Additionally, as both proteins were overexpressed by odontogenic epithelium, their possible roles need to be further explored in benign odontogenic tumors.
Assuntos
Biomarcadores Tumorais/análise , Proteínas do Citoesqueleto/análise , Glicoproteínas de Membrana/análise , Tumores Odontogênicos/química , Adolescente , Adulto , Idoso , Anticorpos Monoclonais , Membrana Celular/química , Criança , Citoplasma/química , Epitélio/química , Feminino , Humanos , Imuno-Histoquímica , Masculino , Neoplasias Mandibulares/química , Neoplasias Mandibulares/patologia , Neoplasias Maxilares/química , Neoplasias Maxilares/patologia , Pessoa de Meia-Idade , Tumores Odontogênicos/patologia , Adulto JovemRESUMO
BACKGROUND: Sonic hedgehog (SHH) pathway activation has been identified as a key factor in the development of many types of tumors, including odontogenic tumors. Our study examined the expression of genes in the SHH pathway to characterize their roles in the pathogenesis of keratocystic odontogenic tumors (KOT) and ameloblastomas (AB). METHODS: We quantified the expression of SHH, SMO, PTCH1, SUFU, GLI1, CCND1, and BCL2 genes by qPCR in a total of 23 KOT, 11 AB, and three non-neoplastic oral mucosa (NNM). We also measured the expression of proteins related to this pathway (CCND1 and BCL2) by immunohistochemistry. RESULTS: We observed overexpression of SMO, PTCH1, GLI1, and CCND1 genes in both KOT (23/23) and AB (11/11). However, we did not detect expression of the SHH gene in 21/23 KOT and 10/11 AB tumors. Low levels of the SUFU gene were expressed in KOT (P = 0.0199) and AB (P = 0.0127) relative to the NNM. Recurrent KOT exhibited high levels of SMO (P = 0.035), PTCH1 (P = 0.048), CCND1 (P = 0.048), and BCL2 (P = 0.045) transcripts. Using immunolabeling of CCND1, we observed no statistical difference between primary and recurrent KOT (P = 0.8815), sporadic and NBCCS-KOT (P = 0.7688), and unicystic and solid AB (P = 0.7521). CONCLUSIONS: Overexpression of upstream (PTCH1 and SMO) and downstream (GLI1, CCND1 and BCL2) genes in the SHH pathway leads to the constitutive activation of this pathway in KOT and AB and may suggest a mechanism for the development of these types of tumors.
Assuntos
Ameloblastoma/genética , Perfilação da Expressão Gênica , Proteínas Hedgehog/genética , Tumores Odontogênicos/genética , Transcrição Gênica/genética , Adolescente , Adulto , Ameloblastoma/química , Ameloblastos/patologia , Ciclina D1/análise , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Proteínas Hedgehog/análise , Humanos , Masculino , Neoplasias Mandibulares/química , Pessoa de Meia-Idade , Mucosa Bucal/química , Recidiva Local de Neoplasia/química , Recidiva Local de Neoplasia/patologia , Tumores Odontogênicos/química , Receptores Patched , Receptor Patched-1 , Proteínas Proto-Oncogênicas c-bcl-2/análise , Receptores de Superfície Celular/análise , Receptores Acoplados a Proteínas G/análise , Proteínas Repressoras/análise , Receptor Smoothened , Fatores de Transcrição/análise , Adulto Jovem , Proteína GLI1 em Dedos de ZincoRESUMO
Leiomyosarcoma (LMS) of the oral cavity, a rare mesenchymal tumor exhibiting smooth-muscle differentiation, is extremely uncommon in childhood. The most frequent location of childhood LMS is the gastrointestinal tract, particularly the stomach. The purpose of this paper is to report a case of leiomyosarcoma affecting the gingival tissues and mandible of a 9-year-old girl with peculiar clinical, microscopic, and radiographic features. Clinical and radiographical examinations revealed a gingival growth affecting the primary mandibular right first molar with inflammatory features. The lesion was initially suspected to be pyogenic granuloma and was removed by excisional biopsy. Microscopic findings showed a hypercellular proliferation of mesenchymal spindle cells, suggesting malignant spindle cell neoplasm. Immunohistochemical, histochemical, and radiographic studies were undertaken, and the final diagnosis established was a low-grade leiomyosarcoma in the gingiva.
Assuntos
Neoplasias Gengivais/patologia , Leiomiossarcoma/patologia , Neoplasias Mandibulares/patologia , Actinas/análise , Criança , Diagnóstico Diferencial , Feminino , Neoplasias Gengivais/química , Humanos , Leiomiossarcoma/química , Neoplasias Mandibulares/químicaRESUMO
Ameloblastoma is a benign epithelial odontogenic tumor and is the most commonly encountered odontogenic tumor in the jaws. Histologically, ameloblastomas occur in different patterns, including plexiform pattern and follicular pattern. "Hybrid" lesion of ameloblastoma is a tumor variant in which histologically, areas of follicular or plexiform ameloblastoma coexist with characteristic areas of ameloblastoma exhibiting pronounced stromal desmoplasia (desmoplastic ameloblastoma). The purpose of this article is to present a case of "hybrid" lesion of desmoplastic ameloblastoma (AD) and conventional, and investigate extracellular matrix proteins such as tenascin, fibronectin, and type I collagen.
Assuntos
Ameloblastoma/patologia , Neoplasias Mandibulares/patologia , Adulto , Ameloblastoma/química , Ameloblastoma/cirurgia , Colágeno Tipo I/análise , Fibronectinas/análise , Humanos , Imuno-Histoquímica , Masculino , Neoplasias Mandibulares/química , Neoplasias Mandibulares/cirurgia , Proteínas de Neoplasias/análise , Tenascina/análiseRESUMO
Rhabdomyosarcoma is the most common soft-tissue sarcoma of the head and neck region in children and adolescents. Oral cavity involvement is relatively uncommon, with tongue, soft palate, hard palate, and buccal mucosa being the sites of predilection. This report presents a rare case of intraosseous oral rhabdomyosarcoma arising in the mandibular bone of a 6-year-old child. Clinical, radiologic, and histopathologic features and possible pathogenesis are discussed.
Assuntos
Neoplasias Mandibulares/patologia , Rabdomiossarcoma Embrionário/patologia , Biomarcadores Tumorais/análise , Criança , Terapia Combinada , Evolução Fatal , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Mandibulares/química , Neoplasias Mandibulares/terapia , Recidiva Local de Neoplasia , Rabdomiossarcoma Embrionário/química , Rabdomiossarcoma Embrionário/terapiaRESUMO
Central granular cell tumor of the jaw was formerly known as granular cell ameloblastic fibroma; recently, the term central granular cell odontogenic fibroma has been proposed. This study attempts to determine the ultrastructural features and selected immunohistochemical properties of the tumor cells. Four formalin-fixed specimens were processed for electron microscopy, and for immunohistochemical staining with antiactin, anti-glial fibrillary acidic protein, and OKT6 (CD1) with the avidin-biotin-peroxidase complex method. Tumor cells contained many primary lysosomes, autophagic vacuoles, and phagocytic vacuoles. The phagocytic vacuoles appeared to contain collagen fibrils. Tumor cells stained positive with antiactin and OKT6 (CD1), and negative with anti-glial fibrillary acidic protein. The results indicate that tumor cells are actively phagocytic and suggest that tumor cells might arise from Langerhans' cells.