RESUMO

Murine hybridoma monoclonal antibodies (MAbs) were produced against the capsular polysaccharide (CPs) of serogroups A, C, W135 and Y meningococci (MenA, MenC, MenW, MenY) in order to develop immunological reagents for the identification of meningococcal polysaccharides. Each serogroup-specific MAb reacted with the CPs from its homologous serogroup only and did not react with CPs from the other three serogroups. The affinity constant (Ka) of the four MAbs measured by non-competitive ELISA was 6.62 × 10(9), 2.76 × 10(9), 1.48 × 10(9) and 3.8 × 10(9) M(-1) for MenA, MenC, MenW and MenY MAbs respectively. The application of these MAbs for identity tests was demonstrated by their abilities to correctly identify the CPs from serogroups A, C, W135 and Y in meningococcal CPs-based vaccines through ELISA. The MAbs obtained in this work are a very valuable set of tools for study meningococcal polysaccharides vaccines.

Assuntos

Anticorpos Antibacterianos , Anticorpos Monoclonais Murinos , Cápsulas Bacterianas , Neisseria meningitidis Sorogrupo A , Neisseria meningitidis Sorogrupo C , Neisseria meningitidis Sorogrupo W-135 , Neisseria meningitidis Sorogrupo Y , Polissacarídeos Bacterianos , Animais , Anticorpos Antibacterianos/química , Anticorpos Antibacterianos/imunologia , Anticorpos Monoclonais Murinos/química , Anticorpos Monoclonais Murinos/imunologia , Especificidade de Anticorpos , Cápsulas Bacterianas/química , Cápsulas Bacterianas/metabolismo , Ensaio de Imunoadsorção Enzimática/métodos , Camundongos , Neisseria meningitidis Sorogrupo A/química , Neisseria meningitidis Sorogrupo A/imunologia , Neisseria meningitidis Sorogrupo C/química , Neisseria meningitidis Sorogrupo C/imunologia , Neisseria meningitidis Sorogrupo W-135/química , Neisseria meningitidis Sorogrupo W-135/imunologia , Neisseria meningitidis Sorogrupo Y/química , Neisseria meningitidis Sorogrupo Y/imunologia , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/imunologia