RESUMO
Murine hybridoma monoclonal antibodies (MAbs) were produced against the capsular polysaccharide (CPs) of serogroups A, C, W135 and Y meningococci (MenA, MenC, MenW, MenY) in order to develop immunological reagents for the identification of meningococcal polysaccharides. Each serogroup-specific MAb reacted with the CPs from its homologous serogroup only and did not react with CPs from the other three serogroups. The affinity constant (Ka) of the four MAbs measured by non-competitive ELISA was 6.62 × 10(9), 2.76 × 10(9), 1.48 × 10(9) and 3.8 × 10(9) M(-1) for MenA, MenC, MenW and MenY MAbs respectively. The application of these MAbs for identity tests was demonstrated by their abilities to correctly identify the CPs from serogroups A, C, W135 and Y in meningococcal CPs-based vaccines through ELISA. The MAbs obtained in this work are a very valuable set of tools for study meningococcal polysaccharides vaccines.
Assuntos
Anticorpos Antibacterianos , Anticorpos Monoclonais Murinos , Cápsulas Bacterianas , Neisseria meningitidis Sorogrupo A , Neisseria meningitidis Sorogrupo C , Neisseria meningitidis Sorogrupo W-135 , Neisseria meningitidis Sorogrupo Y , Polissacarídeos Bacterianos , Animais , Anticorpos Antibacterianos/química , Anticorpos Antibacterianos/imunologia , Anticorpos Monoclonais Murinos/química , Anticorpos Monoclonais Murinos/imunologia , Especificidade de Anticorpos , Cápsulas Bacterianas/química , Cápsulas Bacterianas/metabolismo , Ensaio de Imunoadsorção Enzimática/métodos , Camundongos , Neisseria meningitidis Sorogrupo A/química , Neisseria meningitidis Sorogrupo A/imunologia , Neisseria meningitidis Sorogrupo C/química , Neisseria meningitidis Sorogrupo C/imunologia , Neisseria meningitidis Sorogrupo W-135/química , Neisseria meningitidis Sorogrupo W-135/imunologia , Neisseria meningitidis Sorogrupo Y/química , Neisseria meningitidis Sorogrupo Y/imunologia , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/imunologiaAssuntos
Cooperação Internacional , Meningite Meningocócica/prevenção & controle , Vacinas Meningocócicas , Neisseria meningitidis Sorogrupo A/imunologia , Neisseria meningitidis Sorogrupo C/imunologia , Academias e Institutos , África Subsaariana/epidemiologia , Brasil , Cuba , Países em Desenvolvimento , Custos de Medicamentos , Humanos , Meningite Meningocócica/epidemiologia , Vacinas Meningocócicas/economia , Vacinas Meningocócicas/provisão & distribuiçãoRESUMO
The development of new immune potentiators for human vaccines is an important and expanding field of research. In the present study, the ability of the capsular polysaccharide from Neisseria meningitidis serogroup A (CPS-A), a mannose-containing carbohydrate, to enhance the antibody production against a co-administered model vaccine antigen, is examined. A protein-meningococcal serogroup C capsular polysaccharide (CPS-C) conjugate was selected as the model antigen for this study. After subcutaneous immunization of Balb/C mice, the conjugate mixed with CPS-A induced higher anti-CPS-C IgG and IgG(2a) antibody levels and higher anti-meningococcal serogroup C bactericidal titers than the conjugate alone or mixed with CPS-C. The immuno-stimulatory properties exhibited by CPS-A and the fact that vaccines based on purified CPS-A has been safely used during decades to fight the serogroup A meningococcal disease, support the proposal to use CPS-A as immune potentiator for human vaccination studies.