RESUMO
Probabilistic microbial modelling using logistic regression was used to predict the growth/no growth (G/NG) interfaces of Zygosaccharomyces bailii in simulated acid sauces as a function of natamycin, xanthan gum (XG) and sodium chloride concentrations. The growth was assessed colorimetrically by using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and 2-methoxy-1,4-naphthoquinone as detection reagents. The logistic regression model successfully predicted G/NG probability. The detection reagents used allowed the evaluation of G/NG interfaces in opaque systems with an excellent agreement with the plate count method. Natamycin concentration of 12â¯mg/L was needed to inhibit Z. bailii growth independently of the presence of XG and/or NaCl. Addition of 3.00 and 6.00% of NaCl exerted an antagonistic effect on natamycin action. Furthermore, addition of 0.25 and 0.50% XG decreased natamycin and/or NaCl action. However, an increased in XG concentration to 1.00% decreased yeast growth. Mentioned results highlighted the importance of the correct selection of stress factors applied to inhibit Z. bailii growth.
Assuntos
Antibacterianos/farmacologia , Condimentos/microbiologia , Conservantes de Alimentos/farmacologia , Modelos Biológicos , Natamicina/farmacologia , Polissacarídeos Bacterianos/farmacologia , Cloreto de Sódio/farmacologia , Zygosaccharomyces/efeitos dos fármacos , Antibacterianos/química , Relação Dose-Resposta a Droga , Manipulação de Alimentos , Microbiologia de Alimentos , Conservação de Alimentos , Conservantes de Alimentos/química , Concentração de Íons de Hidrogênio , Modelos Logísticos , Natamicina/química , Polissacarídeos Bacterianos/química , Cloreto de Sódio/química , Fatores de Tempo , Zygosaccharomyces/crescimento & desenvolvimentoRESUMO
In this paper, films based on tapioca starch and containing nisin, natamycin and glycerol were characterized in relation to their physicochemical properties, roughness and hydrophobicity. The content of glycerol affected the mechanical properties of the films studied and the roughness and it was observed an increase in WVP with the increase in glycerol content. The addition of antimicrobials affected the mechanical properties, being nisin the one that produced the greater decrease in the Young modulus. The color was highly affected by the joint presence of natamycin and nisin, which increased the yellow index. The contact angle increased with antimicrobial addition indicating a decrease in hydrophilicity. Nisin also affected the roughness of the films. Water vapor permeability was slightly reduced by the presence of natamycin. It was observed that water vapor permeability and contact angle were correlated with the roughness of the films.