RESUMO

This work investigated the capacity of (-)-epicatechin to prevent the renal damage induced by LPS administration in rats. Male Sprague Dawley rats were fed for 4 days a diet without or with supplementation with (-)-epicatechin (80mg/kg BW/d), and subsequently i.p. injected with lipopolysaccharide (LPS). Six hours after injection, LPS-treated rats exhibited increased plasma creatinine and urea levels as indicators of impaired renal function. The renal cortex of the LPS-treated rats showed: i) increased expression of inflammatory molecules (TNF-α, iNOS and IL-6); ii) activation of several steps of NF-κB pathway; iii) overexpression of TLR4, and iv) higher superoxide anion production and lipid peroxidation index in association with increased levels of gp91phox and p47phox (NOX2) and NOX4. Pretreatment with dietary (-)-epicatechin prevented the adverse effects of LPS challenge essentially by inhibiting TLR4 upregulation and NOX activation and the consequent downstream events, e.g. NF-kB activation.

Assuntos

Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Catequina/farmacologia , Rim/efeitos dos fármacos , Nefrite/prevenção & controle , Administração Oral , Animais , Creatinina/sangue , Regulação da Expressão Gênica , Injeções Intraperitoneais , Interleucina-6/genética , Interleucina-6/imunologia , Rim/imunologia , Rim/patologia , Lipopolissacarídeos , Masculino , NADPH Oxidase 2/genética , NADPH Oxidase 2/imunologia , NADPH Oxidase 4/genética , NADPH Oxidase 4/imunologia , NADPH Oxidases/genética , NADPH Oxidases/imunologia , NF-kappa B/genética , NF-kappa B/imunologia , Nefrite/induzido quimicamente , Nefrite/genética , Nefrite/patologia , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/imunologia , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/imunologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Ureia/sangue