RESUMO
In maternally separated rats, variable chronic stress decreased the emotional reactivity and provoked a state of hypoactivity of the hypothalamic-pituitary-adrenal system at 3 months old but increased its activity after the open field test. The anterodorsal thalami nuclei control of the endocrine response under stress conditions was not manifested however its seems activate grooming behavior. The development of behavioral and endocrine response to stress is influenced by early postnatal environment. On the other hand, the anterodorsal thalami nuclei exert an inhibitory influence on the hypothalamic-pituitary-adrenal system under basal and stressful conditions. The aim of this work is to determine the magnitude of behavioral and hypothalamic-pituitary-adrenal responses to variable chronic stress in adult female rats with anterodorsal thalami nuclei lesions, previously isolated for 4.5 h daily during the first 3 weeks of life. The groups were: non-maternally separated sham and lesioned, maternally separated sham and lesioned with variable chronic stress with and without open field test. At 3 months old, under variable chronic stress, maternal separation provoked an increase in ambulation in sham and lesioned animals (P<0.01) but this parameter was not modified by lesion in either non-maternally separated or maternally separated groups. Neither the lesion nor the maternal separation changed the defecation and rearing parameter. Grooming behavior was lower in maternally separated lesioned rats (P<0.05). Under variable chronic stress maternal separation decreased adrenocorticotrophin hormone in comparison with non-maternally separated (P<0.001) and the lesion did not alter this response. Regarding corticosterone concentrations, maternal separation did not affect this hormone under variable chronic stress conditions and after the open field test there was an increase of this in both non-maternally separated and maternally separated sham and lesioned (P<0.001).
Assuntos
Núcleos Anteriores do Tálamo/fisiopatologia , Comportamento Animal/fisiologia , Sistema Hipotálamo-Hipofisário/fisiopatologia , Privação Materna , Sistema Hipófise-Suprarrenal/fisiopatologia , Estresse Psicológico/fisiopatologia , Hormônio Adrenocorticotrópico/sangue , Hormônio Adrenocorticotrópico/metabolismo , Animais , Núcleos Anteriores do Tálamo/lesões , Doença Crônica , Corticosterona/sangue , Corticosterona/metabolismo , Denervação , Modelos Animais de Doenças , Feminino , Sistema Hipotálamo-Hipofisário/metabolismo , Inibição Neural/fisiologia , Vias Neurais/lesões , Vias Neurais/fisiopatologia , Sistemas Neurossecretores/fisiopatologia , Sistema Hipófise-Suprarrenal/metabolismo , Ratos , Estresse Psicológico/sangue , Estresse Psicológico/etiologia , Regulação para Cima/fisiologiaRESUMO
The hypothalamic-pituitary-adrenal (HPA) axis is normally regulated by extrahypothalamic limbic structures, among these, the anterodorsal thalami nuclei (ADTN), which exert an inhibitory influence on HPA, in basal and acute stress conditions in rats. In the present work we have investigated whether neonatal maternal deprivation (MD) produces long-term changes in the ADTN regulation of HPA activity. Maternal deprivation, in female rats, for 4.5 hs daily, during the first 3 weeks of life, produced at 3 months old, a significant decrease in plasma ACTH concentration (p<0.001) and an increase in plasma corticosterone (C) (p<0.001), compared to control non-deprived rats (NMD). Also MD showed higher plasma epinephrine (E) and norepinephrine (NE) levels than NMD rats. The increase of NE (66.6% p<0.001) was higher than that observed in E (19%). After 30 days of ADTN lesion, plasma ACTH values were higher than in sham lesioned rats, in both NMD and MD animals. ACTH response was greater in MD rats. Plasma C, in NMD, was higher, whereas in MD lesioned animals, it was significantly lower than in sham lesioned. In MD rats, lesion produced a significant increase in plasma E and NE (p<0.001), and again, NE increase was higher than E increase. The more accentuated increase of NE than E, suggests sympathetic nervous system hyperactivity. In summary, neonatal maternal deprivation induces long-term alterations on HPA axis sensitivity and medullo adrenal secretion; enhanced sympathetic nervous system activity and, therefore affected the ADTN inhibitory influence on ACTH and adrenal glands secretion.
Assuntos
Núcleos Anteriores do Tálamo/lesões , Sistema Hipotálamo-Hipofisário/fisiopatologia , Privação Materna , Sistema Hipófise-Suprarrenal/fisiopatologia , Hormônio Adrenocorticotrópico/sangue , Animais , Animais Recém-Nascidos , Núcleos Anteriores do Tálamo/patologia , Corticosterona/sangue , Epinefrina/sangue , Feminino , Norepinefrina/sangue , Distribuição Aleatória , Ratos , Ratos WistarRESUMO
The limbic structures play an important role in the control of the neuroendocrine and sympathical adrenal function in basal and stress conditions. This work was undertaken to evaluate plasma ACTH, adrenocortical activity, cardiac adrenoceptors density and affinity response to variable chronic stress (VCS) in anterodorsal thalamic nuclei (ADTN) lesioned rats. Thirty days after lesion, shamlesioned stressed animals increased plasma ACTH and corticosterone as compared to sham-lesioned unstressed animals (p < 0.05); lesioned rats increased ACTH levels after VCS (p < 0.05) as compared to unstressed-lesioned rats. Whereas in sham-lesion plasma corticosterone (C) increased after stress, in lesioned animals(C) remained unchanged as compared to unstressed-lesioned animals. In the stressed groups, adrenal C contents were below those found in unstressed rats. beta-receptors affinity, in all the experimental groups, was similar, but VCS sham-lesioned animals underwent a significant increase in cardiac D-adrenergic receptors density when compared with basal and lesioned groups (P < 0.001). Our findings would demonstrate that the increment in cardiac beta adrenoceptors density appears as a consequence of the increase in ACTH, plasma corticosterone and sympathetic response provoked by chronic stress situations. ADTN lesion attenuated this hipophisoadrenal system response to chronic stress as well as the above mentioned cardiac beta adrenoceptors density increment.