RESUMO

Mexiletine (MEX), hydroxymethylmexiletine (HMM) and p-hydroxymexiletine (PHM) were analyzed in rat plasma by LC-MS/MS. The plasma samples were prepared by liquid-liquid extraction using methyl-tert-butyl ether as extracting solvent. MEX, HMM, and PHM enantiomers were resolved on a Chiralpak(R) AD column. Validation of the method showed a relative standard deviation (precision) and relative errors (accuracy) of less than 15% for all analytes studied. Quantification limits were 0.5 ng ml(-1) for the MEX and 0.2 ng ml(-1) for the HMM and PHM enantiomers. The validated method was successfully applied to quantify the enantiomers of MEX and its metabolites in plasma samples of rats (n = 6) treated with a single oral dose of racemic MEX.

Assuntos

Cromatografia Líquida de Alta Pressão/métodos , Mexiletina/análogos & derivados , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos , Animais , Antiarrítmicos , Cromatografia Líquida , Meios de Cultura , Medicamentos de Ervas Chinesas/análise , Masculino , Espectrometria de Massas , Mexiletina/análise , Mexiletina/metabolismo , Ratos , Ratos Wistar , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta

Assuntos

Mexiletina/metabolismo , Adulto , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão , Feminino , Humanos , Cinética , Masculino , Mexiletina/sangue , Pessoa de Meia-Idade

Assuntos

Adulto , Humanos , Masculino , Feminino , Mexiletina/metabolismo , Cinética , Disponibilidade Biológica