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J Ind Microbiol Biotechnol ; 32(8): 345-8, 2005 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-15986227

RESUMO

Synthesis of extracellular xylanase in Cellulomonas flavigena is induced in the presence of xylan and sugarcane bagasse as substrates. The essential factors for efficient production of xylanase are the appropriate medium composition and an inducing substrate. The increase in xylanase production levels in C. flavigena were tested with a number of carbon sources and different culture conditions. Xylose, arabinose, glycerol and glucose did not induce xylanase production in this microorganism. beta-Methyl-xyloside (beta-mx), a structural analog of xylobiose, also did not induce xylanase when used as the sole carbon source, but when xylan or sugar cane bagasse was supplemented with beta-mx, extracellular xylanase production increased by 25 or 46%, respectively. The response of C. flavigena to xylan plus beta-mx was accompanied by a significant accumulation of reducing sugar, an effect not observed with the combination sugarcane bagasse plus beta-mx as substrate. To our knowledge, this is the first report on the effect of beta-mx on the induction of xylanase in C. flavigena.


Assuntos
Cellulomonas/enzimologia , Dissacarídeos/metabolismo , Metilglicosídeos/farmacologia , Xilosidases/biossíntese , Cellulomonas/genética , Cellulomonas/crescimento & desenvolvimento , Celulose , Indução Enzimática/efeitos dos fármacos , Xilosidases/genética
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