RESUMO
Objetivo. El objetivo de este estudio fue investigar el potencial efecto antiespasmódico de Rubus roseus y Mentha pulegium en íleon aislado de cobayo (Cavia porcellus) Materiales y Métodos. Los segmentos de íleon se precontrajeron con acetilcolina (ACh; 4 × 10−6 M) y cloruro de potasio (KCl; 4 × 10-4 M) luego se evaluó la respuesta de los decoctos de Rubus roseus (Rr) Mentha pulegium (Mp) en el baño de órganos aislado en concentraciones crecientes (no acumulativas), como controles positivos se utilizaron nifedipino 5 × 10-8 M (antagonista de canales de Ca2+ voltaje dependientes); atropina sulfato 5,5 × 10-9 M (antagonista muscarínico no selectivo), y N-butilbromuro de hioscina 3 x 10-3 M (antagonista muscarínico M3 selectivo). Se cuantificó la amplitud y la frecuencia de contracción (durante diez minutos) después de la administración de los decoctos. Resultados. Los decoctos de Rubus roseus y Mentha pulegium reducen las contracciones inducidas por acetilcolina (p < 0,001) y KCl (p < 0,01) similares a nifedipino (Mp: 4 y 8 mg/mL); atropina sulfato (Rr: 0,2-4 mg/mL; Mp: 0,8 y 1,6 mg/mL) y N-butilbromuro de hioscina (Mp: 2,4 y 8 mg/mL). Por otro lado, ambos decoctos reducen la frecuencia de contracción inducidas por Ach (p < 0,001) y KCl (p < 0,001). Conclusión. Los decoctos de Rubus roseus y Mentha pulegium tienen efecto antiespasmódico en íleon aislado de cobayo (Cavia porcellus).
Objective. This study aimed to investigate the potential antispasmodic effect of Rubus roseus and Mentha pulegium on isolated guinea pig (Cavia porcellus) ileum. Materials and Methods. The ileum segments were pre-contracted with acetylcholine (ACh, 4 × 10-6 M) and potassium chloride (KCl, 4 × 10-4 M) then the response of the Rubus roseus (Rr) and Mentha pulegium (Mp) decocts was evaluated in the organ bath isolated in increasing concentrations (non-cumulative), as positive controls were used nifedipine 5 × 10-8 M (antagonist of voltage dependent Ca2+ channels) atropine sulfate 5,5 × 10-9 M (non-selective muscarinic antagonist) and 3 x 10-3 M hyoscine N-butylbromide (selective M3 muscarinic antagonist). The amplitude and the frequency of contraction were quantified (during 10 minutes) after the administration of the decocts. Results. The decocts of Rubus roseus and Mentha pulegium decrease the contractions induced by acetylcholine (p < 0.001) and KCl (p <0.01) comparable to nifedipine (Mp: 4 and 8 mg/mL), atropine sulfate (Rr: 0.2 - 4 mg/mL, Mp: 0.8 and 1.6 mg/mL) and hyoscine N-butylbromide (Mp: 2.4 and 8 mg/mL). On the other hand, both decocts reduce the frequency of contraction induced by Ach (p <0.001) and KCl (p <0.001). Conclusions. The decocts of Rubus roseus and Mentha pulegium have an antispasmodic effect on isolated guinea pig ileum (Cavia porcellus).
Assuntos
Animais , Parassimpatolíticos , Mentha pulegium , Rubus , Plantas Medicinais , Cobaias , Íleo , Medicina TradicionalRESUMO
The biotransformation of (4R)-(-)-carvone by Mentha pulegium (pennyroyal) leaves and its endophytic bacteria was performed in order to search for novel biocatalysts with enoate reductase activity. The obtained results clearly indicated that endophytes play an important role in the biotransformation of (4R)-(-)-carvone with pennyroyal plant tissues. The best activity was associated to the endophytic bacteria Pseudomonas proteolytica FM18Mci1 and Bacillus sp. FM18civ1. Enoate reductase activity for the reduction of (4R)-(-)-carvone and (4S)-(+)-carvone as model substrates was evaluated for each strain. Finally, both isolated strains were evaluated for the kinetic resolution of racemic carvone. The two bacteria gave (1R, 4R) or (1R, 4S)-dihydrocarvone as major products. P. proteolytica FM18Mci1 had preference for the 4S-(-)-carvone, reaching a conversion 95% in 24 h. In contrast, Bacillus sp. FM18civ1 had preference for (4R)-(-)-carvone. The results obtained in the kinetic resolution of carvone indicated that the Bacillus strain could be useful for resolving a racemic mixture of carvone.
Assuntos
Endófitos/enzimologia , Endófitos/isolamento & purificação , Endófitos/metabolismo , Mentha pulegium/microbiologia , Oxirredutases/metabolismo , Bacillus/enzimologia , Bacillus/isolamento & purificação , Bacillus/metabolismo , Bactérias/enzimologia , Bactérias/isolamento & purificação , Bactérias/metabolismo , Biotransformação , Monoterpenos Cicloexânicos , Endófitos/genética , Cinética , Monoterpenos/química , Monoterpenos/metabolismo , Oxirredução , Filogenia , Folhas de Planta/metabolismo , Folhas de Planta/microbiologia , Pseudomonas/enzimologia , Pseudomonas/isolamento & purificação , Pseudomonas/metabolismo , RNA Ribossômico 16S/genéticaRESUMO
Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances - TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP, potentiate NPSH oxidation and hemolysis. Moreover, both extracts significantly prevents against t-BHP-induced TBARS production. A significant correlation among hemolysis and NPSH levels was found. Taking together, our data points that the association of M. pulegium extracts with t-BHP culminates in toxic effect to exposed erythrocytes, besides its protective effect against t-BHP-induced TBARS production. So, we infer that the use of this extract may exert negative effect during painful crisis in sickle cell anemia. However, more studies are still necessary to better investigate/understand the mechanism(s) involved in the toxic effect resultant from this association.
Assuntos
Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Mentha pulegium/química , Extratos Vegetais/farmacologia , terc-Butil Hidroperóxido/farmacologia , Cromatografia Líquida de Alta Pressão , Humanos , Oxirredução , Estresse Oxidativo , Compostos de SulfidrilaRESUMO
ABSTRACT Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances - TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP, potentiate NPSH oxidation and hemolysis. Moreover, both extracts significantly prevents against t-BHP-induced TBARS production. A significant correlation among hemolysis and NPSH levels was found. Taking together, our data points that the association of M. pulegium extracts with t-BHP culminates in toxic effect to exposed erythrocytes, besides its protective effect against t-BHP-induced TBARS production. So, we infer that the use of this extract may exert negative effect during painful crisis in sickle cell anemia. However, more studies are still necessary to better investigate/understand the mechanism(s) involved in the toxic effect resultant from this association.
Assuntos
Humanos , Extratos Vegetais/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Mentha pulegium/química , terc-Butil Hidroperóxido/farmacologia , Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Oxirredução , Compostos de Sulfidrila , Cromatografia Líquida de Alta Pressão , Estresse OxidativoRESUMO
The objective of this study was to determine the chemical composition, apparent metabolizable energy (AME), AME corrected by nitrogen retention (AMEn) and metabolization coefficients (MC and MCn) of different feeds for broilers from the western region of Paraná State. The experiment was conducted at the Physiology of Birds Laboratory at UNIOESTE. Two hundred and sixty male chicks of the Cobb 500 strain, at 21 days old, were distributed in a randomized design with 12 treatments and four replicates of five birds per experimental unit. The feed ingredients studied were ten different wheat middlings, one triticale, one wheat grain and a reference diet. Values for dry matter, nitrogen, gross energy, neutral detergent fiber, acid detergent fiber, mineral matter, calcium, phosphorus, sodium, potassium and magnesium composition were determined as well as the geometric mean diameter of the feed particles. To determine the apparent metabolizable energy (AME) and AME corrected for nitrogen balance (AMEn) the total excreta collection method was used with growing birds. The chemical composition of the feeds was variable. The values for AME and AMEn ranged from 1. 819-2. 958 and 1. 734-2. 882 kcal. kg-1, respectively. Based on these energetic values and the values for gross energy, the coefficients of metabolizability were calculated. The values ranged from 43. 26-75. 24% for MC and from 42. 28-73. 31% for MCn.(AU)
O objetivo deste trabalho foi determinar a composição química e os valores de energia metabolizável aparente (EMA), EMA corrigida pela retenção de nitrogênio (EMAn) e seus respectivos coeficientes de metabolizabilidade (CMA e CMAn) de diferentes alimentos para frangos de corte provenientes da região oeste do Estado do Paraná. O experimento foi realizado no Laboratório de Fisiologia de Aves da UNIOESTE. Foram utilizados 260 pintos de corte machos, da linhagem Cobb 500, com 21 dias de idade, distribuídos em um delineamento inteiramente casualizado, com doze tratamentos, quatro repetições e cinco aves por unidade experimental. Os ingredientes estudados foram dez diferentes farelos de trigo, um triticale, um trigo grão e uma ração referência. Foram determinados valores de matéria seca, nitrogênio, energia bruta, fibra em detergente neutro, fibra em detergente ácido, matéria mineral, cálcio, fósforo, sódio, potássio e magnésio e o valor do diâmetro geométrico médio. Para determinação dos valores de EMA e EMAn foi utilizado o método de coleta total de excretas com aves em crescimento. A composição química dos alimentos estudados apresentaram variações. Os valores de EMA e EMAn variaram de 1819 a 2958 e 1734 a 2882 kcal/kg, respectivamente. De acordo com os valores energéticos e energia bruta, foram calculados os coeficientes de metabolizabilidade, valores estes que variaram de 43,26 a 75,24% para CMA e de 42,28 a 73,31% para CMAn.(AU)
Assuntos
Mentha pulegium/química , Óleos de Plantas/análise , Antifúngicos/análise , Cladosporium/efeitos dos fármacosRESUMO
O objetivo deste estudo foi comparar o rendimento, a composição química e a atividade antifúngica do óleo essencial de Mentha pulegium (poejo) em diferentes estágios de desenvolvimento, cultivada sob condições controladas no sul do Brasil. A hidrodestilação de folhas frescas de M. pulegium, coletadas aos 60, 70 e 85 dias após o transplante, apresentou rendimento de óleo essencial de 0,17 %, 0,23 % e 0,17 %, respectivamente. Todas as amostras de óleo apresentaram atividade contra Cladosporium herbarum. Através das análises de cromatografia gasosa (GC) e GC/MS do óleo essencial, foram identificados onze constituintes, sete (mentona, isomentona, neoisomentol, pulegona, piperitona, 1.1- dimetoxi-2- nonino e piperitenona) comuns às três amostras, enquanto dois outros (mentofurano, mirtenal) foram detectados somente nas amostras da primeira e segunda coletas. A pulegona foi o principal constituinte nas duas primeiras amostras (26,65 %), seguida pela piperitenona (20,41; 12,60 %). A concentração de pulegona aumentou para 31,05 % na última coleta, porém o constituinte majoritário foi a piperitenona (36,32 %). Os resultados demonstraram que o óleo essencial de M. pulegium apresenta potencial como agente antifúngico e sua composição química depende do estágio de desenvolvimento da planta.(AU)
The aim of this study was to compare the yield, chemical composition and antifungal activity of the essential oil that is obtained from Mentha pulegium in different developmental stages and cultivated under controlled conditions in southern Brazil. The hydrodistillation of fresh leaves that were collected at 60, 70 and 85 days resulted inessential oil yields of 0.17 %, 0.23 % and 0.17 %, respectively. All of the essential oil samples showed antifungal activity against Cladosporium herbarum. The gas chromatograph (GC) and GC/MS analysis revealed eleven constituents: seven (pulegone, piperitenone, menthone, isomenthone, neoisomenthol, piperitone and 1.1-dimethoxy-2-nonyne) were common to three samples, while menthofuran and myrtenal were detected only in samples of the first and second stages. Pulegone was the main constituent of the essential oil samples from the first and second stages (26.65 %), followed by piperitenone (20.41; 12.60 %). The pulegone concentration increased to 31.05 % in the last collection, while the major constituent was piperitenone (36.32 %). In conclusion, the results demonstrated that M. pulegium essential oil presents potential as an antifungal agent, and its chemical composition depends on the stage of development during which it was extracted.(AU)
Assuntos
Mentha pulegium/química , Óleos de Plantas/análise , Antifúngicos/análise , Cladosporium/efeitos dos fármacosRESUMO
O objetivo deste estudo foi comparar o rendimento, a composição química e a atividade antifúngica do óleo essencial de Mentha pulegium (poejo) em diferentes estágios de desenvolvimento, cultivada sob condições controladas no sul do Brasil. A hidrodestilação de folhas frescas de M. pulegium, coletadas aos 60, 70 e 85 dias após o transplante, apresentou rendimento de óleo essencial de 0,17 %, 0,23 % e 0,17 %, respectivamente. Todas as amostras de óleo apresentaram atividade contra Cladosporium herbarum. Através das análises de cromatografia gasosa (GC) e GC/MS do óleo essencial, foram identificados onze constituintes, sete (mentona, isomentona, neoisomentol, pulegona, piperitona, 1.1- dimetoxi-2- nonino e piperitenona) comuns às três amostras, enquanto dois outros (mentofurano, mirtenal) foram detectados somente nas amostras da primeira e segunda coletas. A pulegona foi o principal constituinte nas duas primeiras amostras (26,65 %), seguida pela piperitenona (20,41; 12,60 %). A concentração de pulegona aumentou para 31,05 % na última coleta, porém o constituinte majoritário foi a piperitenona (36,32 %). Os resultados demonstraram que o óleo essencial de M. pulegium apresenta potencial como agente antifúngico e sua composição química depende do estágio de desenvolvimento da planta.
The aim of this study was to compare the yield, chemical composition and antifungal activity of the essential oil that is obtained from Mentha pulegium in different developmental stages and cultivated under controlled conditions in southern Brazil. The hydrodistillation of fresh leaves that were collected at 60, 70 and 85 days resulted inessential oil yields of 0.17 %, 0.23 % and 0.17 %, respectively. All of the essential oil samples showed antifungal activity against Cladosporium herbarum. The gas chromatograph (GC) and GC/MS analysis revealed eleven constituents: seven (pulegone, piperitenone, menthone, isomenthone, neoisomenthol, piperitone and 1.1-dimethoxy-2-nonyne) were common to three samples, while menthofuran and myrtenal were detected only in samples of the first and second stages. Pulegone was the main constituent of the essential oil samples from the first and second stages (26.65 %), followed by piperitenone (20.41; 12.60 %). The pulegone concentration increased to 31.05 % in the last collection, while the major constituent was piperitenone (36.32 %). In conclusion, the results demonstrated that M. pulegium essential oil presents potential as an antifungal agent, and its chemical composition depends on the stage of development during which it was extracted.
Assuntos
Antifúngicos/análise , Cladosporium/efeitos dos fármacos , Mentha pulegium/química , Óleos de Plantas/análiseRESUMO
The objective of this study was to determine the chemical composition, apparent metabolizable energy (AME), AME corrected by nitrogen retention (AMEn) and metabolization coefficients (MC and MCn) of different feeds for broilers from the western region of Paraná State. The experiment was conducted at the Physiology of Birds Laboratory at UNIOESTE. Two hundred and sixty male chicks of the Cobb 500 strain, at 21 days old, were distributed in a randomized design with 12 treatments and four replicates of five birds per experimental unit. The feed ingredients studied were ten different wheat middlings, one triticale, one wheat grain and a reference diet. Values for dry matter, nitrogen, gross energy, neutral detergent fiber, acid detergent fiber, mineral matter, calcium, phosphorus, sodium, potassium and magnesium composition were determined as well as the geometric mean diameter of the feed particles. To determine the apparent metabolizable energy (AME) and AME corrected for nitrogen balance (AMEn) the total excreta collection method was used with growing birds. The chemical composition of the feeds was variable. The values for AME and AMEn ranged from 1. 819-2. 958 and 1. 734-2. 882 kcal. kg-1, respectively. Based on these energetic values and the values for gross energy, the coefficients of metabolizability were calculated. The values ranged from 43. 26-75. 24% for MC and from 42. 28-73. 31% for MCn.
O objetivo deste trabalho foi determinar a composição química e os valores de energia metabolizável aparente (EMA), EMA corrigida pela retenção de nitrogênio (EMAn) e seus respectivos coeficientes de metabolizabilidade (CMA e CMAn) de diferentes alimentos para frangos de corte provenientes da região oeste do Estado do Paraná. O experimento foi realizado no Laboratório de Fisiologia de Aves da UNIOESTE. Foram utilizados 260 pintos de corte machos, da linhagem Cobb 500, com 21 dias de idade, distribuídos em um delineamento inteiramente casualizado, com doze tratamentos, quatro repetições e cinco aves por unidade experimental. Os ingredientes estudados foram dez diferentes farelos de trigo, um triticale, um trigo grão e uma ração referência. Foram determinados valores de matéria seca, nitrogênio, energia bruta, fibra em detergente neutro, fibra em detergente ácido, matéria mineral, cálcio, fósforo, sódio, potássio e magnésio e o valor do diâmetro geométrico médio. Para determinação dos valores de EMA e EMAn foi utilizado o método de coleta total de excretas com aves em crescimento. A composição química dos alimentos estudados apresentaram variações. Os valores de EMA e EMAn variaram de 1819 a 2958 e 1734 a 2882 kcal/kg, respectivamente. De acordo com os valores energéticos e energia bruta, foram calculados os coeficientes de metabolizabilidade, valores estes que variaram de 43,26 a 75,24% para CMA e de 42,28 a 73,31% para CMAn.
Assuntos
Antifúngicos/análise , Cladosporium/efeitos dos fármacos , Mentha pulegium/química , Óleos de Plantas/análiseRESUMO
OBJECTIVES: We evaluated the relaxant activity of the essential oil of Mentha pulegium L. (EOMP) and pulegone in rat isolated tracheal and bladder smooth muscles. METHODS: ISOMETRIC contractions of isolated tracheal and bladder strips from male Wistar rats were induced by KCl (K60; 60 mm) or acetylcholine (ACh; 10 µm). EOMP and its majory compound pulegone were incubated, after contracting agent, with the tissues in cumulating concentrations. KEY FINDINGS: EOMP (3-300 µg/ml) inhibited the contractions induced by ACh and K60 in both tissues, but was more effective against the contractions induced by K60 in trachea (IC50 = 40.47 ± 3.27 µg/ml) compared with ACh. Its relaxant action rules out ganglia and NO participation. Pulegone (10(-7) to 10(-3 ) m) inhibited the contractions induced by ACh and K60 in both tissues. EOMP concentration-dependently inhibited the contractions evoked by addition of CaCl(2) in depolarised trachea, suggesting inhibition of extracellular calcium entry. CONCLUSIONS: These findings suggests that EOMP induced relaxant responses in pre-contracted smooth muscles of rat trachea and bladder, which are likely to be mediated via inhibition of calcium entry, mainly by its major compound, pulegone. These effects are coherent with the popular use of EOMP as an antispasmodic agent.
Assuntos
Mentha pulegium/química , Contração Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Óleos Voláteis/farmacologia , Parassimpatolíticos/farmacologia , Traqueia/efeitos dos fármacos , Bexiga Urinária/efeitos dos fármacos , Acetilcolina , Animais , Cloreto de Cálcio/metabolismo , Monoterpenos Cicloexânicos , Relação Dose-Resposta a Droga , Concentração Inibidora 50 , Masculino , Monoterpenos/farmacologia , Músculo Liso/fisiologia , Extratos Vegetais/farmacologia , Cloreto de Potássio , Ratos , Ratos Wistar , Traqueia/fisiologia , Bexiga Urinária/fisiologiaRESUMO
The aim of the present work was to determine the in vitro effect of Mentha piperita and Mentha pulegium essential oils against Echinococcus granulosus and to compare the effectiveness of both oils according to the exposure time and concentration. Although both treatments had a protoscolicidal effect, M. pulegium had a considerably stronger effect than M. piperita. Essential oil of M. pulegium produced dose- and time-dependent effects. Maximal protoscolicidal effect was observed after 12 days of incubation and reached 0% after 18 days. This lack of viability was proved during the determination of infectivity into mice. Essential oil of M. piperita produced only a time-dependent effect. At 24 days p.i., the viability of protoscoleces decreased to approximately 50%. Scanning and transmission electron microscopy (SEM and TEM) demonstrated the drug-induced ultrastructural damage. On the other hand, a loss of turgidity was detected in all M. pulegium-treated cysts respective of the drug concentration. There was a correlation between the intensity of damage and the concentration of the essential oil assayed. Studies by SEM revealed that the germinal layer of treated cysts lost the feature multicellular structure. M. pulegium essential oil showed piperitone oxide as main compound in their composition, and we suggest that this component could be responsible of the markedly anthelmintic effect detected. Our data suggest that essential oils of Mentha spp. can be a promising source of potential protoscolicidal agents. The isolation of active anthelmintic constituents is in progress and may lead to the discovery of compounds with improved therapeutic value.
Assuntos
Anti-Helmínticos/farmacologia , Echinococcus granulosus/efeitos dos fármacos , Echinococcus granulosus/crescimento & desenvolvimento , Mentha piperita/química , Mentha pulegium/química , Óleos Voláteis/farmacologia , Animais , Anti-Helmínticos/química , Relação Dose-Resposta a Droga , Equinococose/tratamento farmacológico , Equinococose/parasitologia , Echinococcus granulosus/patogenicidade , Echinococcus granulosus/ultraestrutura , Feminino , Camundongos , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Óleos Voláteis/química , Testes de Sensibilidade Parasitária , Fitoterapia , Óleos de Plantas/química , Óleos de Plantas/farmacologia , Especificidade da Espécie , Análise de Sobrevida , Fatores de TempoRESUMO
Nesse trabalho foi investigado a composição química de óleos essenciais de duas plantas medicinais cultivadas em Ilhéus, Brasil, Plectranthus amboinicus (Lour.) Spreng. (hortelã-grosso) e Mentha pulegium L. (poejo). Os óleos essenciais foram extraídos das folhas frescas por hidrodestilação e foram analisados por CG/FID e CG-EM, na primavera e no inverno. P. amboinicus forneceu, tanto na primavera como no inverno, 0,10 por cento de óleo sendo timol o componente majoritário. M. pulegium forneceu na primavera 0,20 por cento de óleo e no inverno 0,09 por cento. Os componentes majoritários dos óleos foram pulegona e trans-cariofileno; borneol, mentol e piperitona foram identificados em menores quantidades. No inverno foram observados maiores quantidades de mentol e isomentol. Acetatos de neoisomentila, de mentila e de isometila foram observados somente no inverno. Esse trabalho contribui para o conhecimento das espécies vegetais cultivadas na região do sul da Bahia.
This work investigated the chemical composition of the essential oils of two medicinal plants grown in Ilhéus Municipality, Bahia State, Brazil: Plectranthus amboinicus (Lour.) Spreng (Mexican mint) and Mentha pulegium L. (pennyroyal). The essential oils were extracted from fresh leaves by hydrodistillation and analyzed by GC-FID and GC-MS, in the spring and in the winter. In both seasons, P. amboinicus yielded 0.10 percent oil and had thymol as major component. M. pulegium yielded 0.20 percent and 0.09 percent oil in the spring and winter, respectively. The major components were pulegone and trans-caryophyllene, whereas borneol, menthol and piperitone were identified at small quantities. Higher menthol and isomenthol levels were detected in the winter. In addition, neoisomenthyl, menthyl and isomenthyl acetates were only observed in the winter. This work contributes to the knowledge of plant species grown in southern Bahia.
Assuntos
Mentha pulegium , Óleos Voláteis/química , Plectranthus , Brasil , Folhas de Planta/química , Lamiaceae/química , Plantas Medicinais , Estações do AnoRESUMO
In a rural population of Bulnes, Bio-Bio Region of Chile, it was assessed the daily consumption of medicinal plants and drugs. Through 168 interviews the consumption of 5 medicinal plants was evaluated, considering their application, used plant parts, type of consumption, preparation, age range of people consuming mainly medicinal plants, use of other pharmacological treatments, main diseases present in the population under study and the type of drugs consumed. The obtained results demonstrate that higher consumed medicinal plants were peppermint (Mentha piperita) chamomile (Matricaria recutita), rute (Ruta graveolens), oregano (Origanum vulgare) and pennyroyal (Mentha pulegium). The consumption of medicinal plants was mainly complementary. The therapeutic use of medicinal plants in the rural population of Bulnes, is mainly digestive and it is not necessarily related to those diseases with more prevalence or most commonly observed in the population. It is important to consider future studies to determine if medicinal plants are more likely used to reduce the digestive discomfort which arises as a lateral damage of pharmaceutical drugs.
En una población rural de la Comuna de Bulnes, VIII región de Chile, fue valorado el consumo de plantas medicinales y fármacos. A través de 168 encuestas fue valorado el consumo de 5 plantas medicinales, su aplicación, las partes mas usadas de las plantas, tipo de consumo, preparación, porcentaje del rango etáreo que consume mayoritariamente plantas medicinales, porcentaje de la población sin y con consumo de fármacos, el porcentaje de las enfermedades presentes en la población en estudio y de los fármacos consumidos. Se obtuvo que las plantas medicinales más consumidas fueron la menta (Mentha piperita), manzanilla (Matricaria recutita), ruda (Ruta graveolens), orégano (Origanum vulgare) y poleo (Mentha pulegium). El consumo de las plantas medicinales fue principalmente de tipo complementario. El uso terapéutico de las plantas medicinales en la población rural de Bulnes, es principalmente de tipo digestivo no relacionándose con las enfermedades de mayor presencia en la población. Dicho uso es probablemente para disminuir las molestias digestivas causadas por el consumo de fármacos. Es necesario realizar mas estudios tendientes a esclarecer este punto de importancia relevante.
Assuntos
Humanos , Recém-Nascido , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso de 80 Anos ou mais , Preparações Farmacêuticas , Plantas Medicinais , Terapias Complementares , Chile , Coleta de Dados , Família , Matricaria , Mentha piperita , Mentha pulegium , Origanum , Zona Rural , RutaRESUMO
Mentha pulegium is common known as "poleo" and used for the treatment of diarrhea, headache and cough in Mexican traditional medicine. Organic extracts from aerial parts were evaluated to determine their spasmolytic action on rat isolated ileum test. Hexanic (HEMp), dichloromethanic (DEMp) and methanolic (MEMp) extracts induced a concentration-dependent (0.97 to 1000 microg/mL) antispasmodic effect on spontaneous contractions. DEMp was the most active extract; therefore, spasmolytic mechanism was investigated. This extract (200 microg/mL) induced a significant depression on cumulative concentration-response curve for carbachol and serotonin (P<0.05). Besides, extract decreased and shifted to the right KCl- and CaCl2-induced contraction curves. Moreover, pre-incubation with chlorpromazine (0.001 mM) shifted to the left the relaxant curve. Pre-treatment with L-NAME (1 mM), papaverine (0.01 mM), teophylline (0.01 mM), TEA (1 mM) and glybenclamide (0.1 mM) did not produced any changed of the relaxant curves of DEMp. Findings indicate that dichloromethanic extract of M. pulegium induced its spasmolytic effect through Ca2+-influx blockade, which may explain its traditional use against diarrhea.
Assuntos
Íleo/efeitos dos fármacos , Íleo/fisiologia , Parassimpatolíticos/farmacologia , Animais , Masculino , Mentha pulegium , México , NG-Nitroarginina Metil Éster/farmacologia , Ratos , Ratos WistarRESUMO
The repeated use of permethrin and other insecticides for the control of head lice, Pediculus humanus capitis De Geer (Anoplura: Pediculidae), during past decades has resulted in the development of marked levels of resistance. Thus, new alternative insecticides are needed for the control of head lice. We studied the fumigant and repellent properties of essential oils from 16 native and exotic plants in Argentina, and 21 chemical components against permethrin-resistant head lice from Argentina. With a direct vapor-exposure bioassay, the most effective oil was from the native Myrcianthes cisplatensis Cambess (Myrtaceae) with a time to 50% knockdown (KT50) of 1.3 min, followed by exotic species, Eucalyptus cinerea F.V. Muell., Eucalyptus viminalis Labill., and Eucalyptus saligna Smith. with KT50 values of 12.0, 14.9, and 17.4 min, respectively. The most effective components were 1,8-cineole and anisole, with KT50 values of 11.1 and 12.7 min, respectively. Regression analysis of KT50 values and vapor pressures and water-partition coefficients for the essential oil components revealed that the most effective fumigants were among the more volatile components. Repellency assays indicated that the essential oil from Mentha pulegium L. and its benzyl alcohol component were the most effective repellents, having repellency indices of 75.5 and 57.8%, respectively. Thus, some Argentinean plants contain essential oils and components that function as fumigants or as repellents and thereby show potential for development of new control products for head lice.
Assuntos
Controle de Insetos/métodos , Repelentes de Insetos/farmacologia , Óleos Voláteis/farmacologia , Pediculus/efeitos dos fármacos , Óleos de Plantas/farmacologia , Animais , Argentina , Bioensaio/métodos , Criança , Fumigação/métodos , Humanos , Controle de Insetos/normas , Repelentes de Insetos/administração & dosagem , Repelentes de Insetos/química , Resistência a Inseticidas , Infestações por Piolhos/parasitologia , Infestações por Piolhos/prevenção & controle , Mentha pulegium/química , Óleos Voláteis/química , Óleos de Plantas/administração & dosagem , Óleos de Plantas/química , VolatilizaçãoRESUMO
The effects of the essential oil of Mentha pulegium L. (EOMP), a plant commonly known as "pennyroyal" or "poejo" that is used in folk medicine as an abortifaceant, were assessed on the isolated rat myometrium. Myometrial strips were stimulated with 10 nM oxytocin or 10 microM PGF (2alpha). EOMP (10 - 300 microg/mL) concentration-dependently and reversibly inhibited the amplitude of oscillatory contractions, being approximately 3-fold more active against contractions stimulated by oxytocin than those by PGF (2alpha) (IC (50) values of 45.7 +/- 5.6 microg/mL and 160.9 +/- 5.9 microg/mL , respectively), although the maximal inhibitory effect occurred at the same concentration (300 microg/mL ) in both cases. This action was shared by pulegone (30 - 300 microM), the principal component of the essential oil (IC (50) values of 21.8 +/- 2.1 microg/mL and 12.7 +/- 4.6 microg/mL , respectively). Nifedipine (30 nM - 30 microM) also abolished agonist-stimulated contractions, and was approximately twice and 12 times as potent as EOMP in inhibiting oxytocin- and prostaglandin F (2alpha) (PGF (2alpha))-stimulated contractions, respectively. In conclusion, our results show that the essential oil of the abortifaceant plant Mentha pulegium exerts an inhibitory effect on the contractile activity of the isolated rat myometrium. This oil shares a common effect with the voltage-dependent calcium channel (VDCC) blocker nifedipine, although ostensibly acting via a different mechanism. It thus appears that EOMP and pulegone do not exert direct toxic effects on the myometrium per se that would cause abortion, and other possibilities such as systemic metabolism of plant constituents may rather underlie the abusive use of Mentha pulegium in popular medicine.
Assuntos
Mentha pulegium , Miométrio/efeitos dos fármacos , Fitoterapia , Óleos de Plantas/farmacologia , Tocolíticos/farmacologia , Contração Uterina/efeitos dos fármacos , Animais , Monoterpenos Cicloexânicos , Relação Dose-Resposta a Droga , Feminino , Concentração Inibidora 50 , Monoterpenos/farmacologia , Miométrio/fisiologia , Nifedipino/farmacologia , Óleos de Plantas/administração & dosagem , Óleos de Plantas/uso terapêutico , Ratos , Ratos Wistar , Tocolíticos/administração & dosagem , Tocolíticos/uso terapêuticoRESUMO
O híbrido Mentha pulegium x spicata, também conhecido como poejo de praia, é uma planta perene, rasteira, ramosa, com caule arroxeado, folhas opostas, lanceoladas e serreadas no bordo, apresentando odor característico. O objetivo do presente trabalho foi realizar um estudo da anatomia foliar, incluindo um estudo de biometria tecidual e análise quantitativa de tricomas secretores/mm2 e de estômatos/mm2 em ambas as faces da folha, além de uma análise química do óleo essencial. Foram efetuadas lâminas permanentes para análise anatômica do limbo foliar e também lâminas da impressão foliar para a contagem do número de tricomas e estômatos. A análise química foi efetuada através da extração do óleo essencial por arraste à vapor d'água e analisado por CG-EM. Os tricomas capitados e peltados estão presentes em ambas as faces da folha, porém predominam na superfície abaxial. A análise de variância mostrou que há diferenças significativas para o número de tricomas capitados e tectores entre as superfícies adaxial e abaxial, mas não são significativas para o tricoma peltado. O óleo essencial analisado, mostra a presença do componente majoritário, trans-epóxido de piperitona, responsável por mais de 80% da composição relativa no óleo bruto
Assuntos
Folhas de Planta/anatomia & histologia , Folhas de Planta/fisiologia , Folhas de Planta/química , Lamiaceae , Mentha pulegium , Biometria , Óleos Voláteis/análiseRESUMO
Por las indicaciones que hacen notables profesores al respecto de este vegetal. se ve pues que con el tiempo está llamado a ocupar un lugar preferente entre los remedios que emplea la terapéutica para curar varias enfermedades del estómago, razón por la cual vuelvo a repetir he creído conveniente ocuparme sobre el particular, mas tal vez lo encuentre rudimentario y deficiente, no obstante no haber omitido esfuerzo alguno para hacer lo mejor posible, teniendo siempre que lamentar que cuando uno trata de hacer cualquier trabajo de esta naturaleza, se encuentra a cada momento con dificultades dada la escasez de medios y material científico de que no disponemos en nuestro país.(AU)