RESUMO
DNA polymeric films (DNA-PFs) are a promising drug delivery system (DDS) in modern medicine. In this study, we evaluated the growth behavior of oral squamous cell carcinoma (OSCC) cells on DNA-PFs. The morphological, biochemical, and cytometric features of OSCC cell adhesion on DNA-PFs were also assessed. An initial, temporary alteration in cell morphology was observed at early time points owing to the inhibition of cell attachment to the film, which then returned to a normal morphological state at later time points. MTT and resazurin assays showed a moderate reduction in cell viability related to increased DNA concentration in the DNA-PFs. Flow cytometry studies showed low cytotoxicity of DNA-PFs, with cell viabilities higher than 90% in all the DNA-PFs tested. Flow cytometric cell cycle analysis also showed average cell cycle phase distributions at later time points, indicating that OSCC cell growth is maintained in the presence of DNA-PFs. These results show high biocompatibility of DNA-PFs and suggest their use in designing "dressing material," where the DNA film acts as a support for cell growth, or with incorporation of active or photoactive compounds, which can induce tissue regeneration and are useful to treat many diseases, especially oral cancer.
Assuntos
Proliferação de Células , DNA/química , Membranas Artificiais , Polímeros/química , Medicina Regenerativa , Técnicas de Cultura de Tecidos/instrumentação , Alicerces Teciduais/química , Materiais Biocompatíveis/análise , Materiais Biocompatíveis/farmacologia , Carcinoma de Células Escamosas/patologia , Linhagem Celular Tumoral , Sobrevivência Celular , DNA/farmacologia , Humanos , Teste de Materiais , Neoplasias Bucais/patologia , Polímeros/farmacologia , Medicina Regenerativa/instrumentação , Medicina Regenerativa/métodos , Técnicas de Cultura de Tecidos/métodosRESUMO
El tratamiento endodóntico convencional en casos de dientes con desarrollo radicular incompleto y periodontitis apical incluye opciones como la cirugía endodóntica o laapexificación mediante el uso de hidróxido de calcio o del compuesto de minerales trióxido. Sin embargo, numerosos ensayos ex vivo e in vivo en modelos animales, así como estudios clínicos en humanos, han demostrado que, luego de una adecuada desinfección y la formación de un coágulo sanguíneo, la posibilidad de obtener la regeneración de los tejidos infectados dentro del espacio del sistema de conductos radiculares permitiendo a su vez la continuación del desarrollo de la raíz en DDRI con periodontitis apical es actualmente una realidad con evidencia científicacomprobada. En ese sentido, la combinación de tres antibióticos talescomo metronidazol, ciprofloxacina y minociclina conocida como pasta triple antibiótica ha demostrado ser muy efectiva para obtener el nivel de desinfección necesaria. El propósito del presente estudio fue analizar la bibliografía referida al rol de la pasta triple antibiótica en endodoncia regenerativa para el tratamiento de dientes con desarrollo radicular incompleto con periodontitis apical.
Assuntos
Adolescente , Pré-Escolar , Criança , Periodontite Periapical/tratamento farmacológico , Medicina Regenerativa/instrumentação , Medicina Regenerativa/métodos , Materiais Restauradores do Canal Radicular/farmacologia , Materiais Restauradores do Canal Radicular/uso terapêutico , Ápice Dentário/fisiologia , Antibacterianos/classificação , Antibacterianos/uso terapêutico , Combinação de Medicamentos , Dente Decíduo , Dentição PermanenteRESUMO
Adhesion and Viability study with human dental pulp stem cell using natural nanotolith/bacterial cellulose scaffolds for regenerative medicine are presented at first time in this work. Nanotolith, are osteoinductors, i.e., they stimulate bone regeneration, enabling higher cells migration for bone tissue regeneration formation. This is mainly because nanotoliths are rich minerals present in the internal ear of bony fish. In addition, are part of a system which acts as a depth sensor and balance, acting as a sound vibrations detector and considered essential for the bone mineralization process, as in hydroxiapatites. Nanotoliths influence in bacterial cellulose was analyzed using transmission infrared spectroscopy (FTIR). Results shows that fermentation process and nanotoliths agglomeration decrease initial human dental pulp stem cell adhesion however tested bionanocomposite behavior has cell viability increase over time.