RESUMO
Minimally invasive transcatheter interventional therapy utilizing cardiac occluders represents the primary approach for addressing congenital heart defects and left atrial appendage (LAA) thrombosis. However, incomplete endothelialization and delayed tissue healing after occluder implantation collectively compromise clinical efficacy. In this study, we have customized a recombinant humanized collagen type I (rhCol I) and developed an rhCol I-based extracellular matrix (ECM)-mimetic coating. The innovative coating integrates metal-phenolic networks with anticoagulation and anti-inflammatory functions as a weak cross-linker, combining them with specifically engineered rhCol I that exhibits high cell adhesion activity and elicits a low inflammatory response. The amalgamation, driven by multiple forces, effectively serves to functionalize implantable materials, thereby responding positively to the microenvironment following occluder implantation. Experimental findings substantiate the coating's ability to sustain a prolonged anticoagulant effect, enhance the functionality of endothelial cells and cardiomyocyte, and modulate inflammatory responses by polarizing inflammatory cells into an anti-inflammatory phenotype. Notably, occluder implantation in a canine model confirms that the coating expedites reendothelialization process and promotes tissue healing. Collectively, this tailored ECM-mimetic coating presents a promising surface modification strategy for improving the clinical efficacy of cardiac occluders.
Assuntos
Materiais Revestidos Biocompatíveis , Matriz Extracelular , Cicatrização , Animais , Matriz Extracelular/metabolismo , Cães , Humanos , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Cicatrização/efeitos dos fármacos , Colágeno Tipo I/metabolismo , Materiais Biomiméticos/química , Materiais Biomiméticos/farmacologia , Células Endoteliais da Veia Umbilical Humana , Reepitelização/efeitos dos fármacos , Adesão Celular/efeitos dos fármacosRESUMO
The (002) crystallographic plane-oriented hydroxyapatite (HA) and anatase TiO2 enable favorable hydrophilicity, osteogenesis, and biocorrosion resistance. Thus, the crystallographic plane control in HA coating and crystalline phase control in TiO2 is vital to affect the surface and interface bioactivity and biocorrosion resistance of titanium (Ti) implants. However, a corresponding facile and efficient fabrication method is absent to realize the HA(002) mineralization and anatase TiO2 formation on Ti. Herein, we utilized the predominant Ti(0002) plane of the fibrous-grained titanium (FG Ti) to naturally form anatase TiO2 and further achieve a (002) basal plane oriented nanoHA (nHA) film through an in situ mild hydrothermal growth strategy. The formed FG Ti-nHA(002) remarkably improved hydrophilicity, mineralization, and biocorrosion resistance. Moreover, the nHA(002) film reserved the microgroove-like topological structure on FG Ti. It could enhance osteogenic differentiation through promoted contact guidance, showing one order of magnitude higher expression of osteogenic-related genes. On the other hand, the nHA(002) film restrained the osteoclast activity by blocking actin ring formation. Based on these capacities, FG Ti-nHA(002) improved new bone growth and binding strength in rabbit femur implantation, achieving satisfactory osseointegration within 2 weeks.
Assuntos
Durapatita , Osseointegração , Titânio , Titânio/química , Durapatita/química , Animais , Osseointegração/efeitos dos fármacos , Coelhos , Osteogênese/efeitos dos fármacos , Corrosão , Camundongos , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Diferenciação Celular/efeitos dos fármacosRESUMO
BACKGROUND: Drug-coated balloons (DCBs) angioplasty is safe and effective for coronary artery disease. However, DCBs dilatation for the treatment of benign esophageal strictures is rarely reported. PURPOSE: We aimed to report the clinical outcomes of DCBs dilatation for patients with benign esophageal strictures. METHODS: From May 2020 to August 2023, 18 patients underwent DCBs dilatation for benign esophageal strictures. Baseline demographics were recorded and evaluated, including gender, age, comorbidities, stricture diameter and length, dilatation session, complications. RESULTS: A total of 24 dilatation sessions of DCBs were performed, with a mean of 1.3 ± 0.6 sessions per patients (range 1.0-5.0). Dysphagia score decreased significantly after DCBs dilatation (2.6 ± 1.1 vs. 0.9 ± 1.3, p = 0.0002). Both stricture diameter and stricture index decreased significantly after DCBs dilatation (p < 0.0001). No procedure-related death, massive bleeding or esophageal perforation was observed during or after DCBs dilatation. Minor complications were found in only 3 patients (16.7%). All 18 patients were successfully followed up for a median period of 12.0 months. By the end of follow up, 10 patients showed no dysphagia, 6 patients showed mild dysphagia and 2 patients showed no improvement in dysphagia. The clinical success rate of DCBs dilatation is 88.9%. CONCLUSION: DCBs dilatation may be a safe, effective and feasible treatment for benign esophageal strictures, and can be utilized as an alternative option after standard dilatation has failed. Prospective studies with large samples are needed to further validate its clinical efficacy.
Assuntos
Transtornos de Deglutição , Dilatação , Estenose Esofágica , Humanos , Estenose Esofágica/terapia , Estenose Esofágica/etiologia , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Dilatação/métodos , Dilatação/instrumentação , Resultado do Tratamento , Transtornos de Deglutição/terapia , Transtornos de Deglutição/etiologia , Estudos Retrospectivos , Materiais Revestidos Biocompatíveis , Adulto , Angioplastia com Balão/métodos , Idoso de 80 Anos ou maisRESUMO
PURPOSE: To compare the endothelial coverage of different stents in porcine carotid arteries. Research problem: How effective are polyurethane stents (PU) and PU + rapamycin (PU + RAPA) compared to bare-metal stents on endothelial coverage by neointima in pigs after 28 days? METHODS: The methodology had two phases for an interventional, experimental, prospective study, with three Moura pigs, 12 weeks old and weighing between 19 and 22.5 kg. In phase I, eight stents were implanted in carotid arteries; three stents coated with PU, three coated with PU + RAPA, and two without coating. After 28 days, phase II was carried out, consisting of euthanasia, removal of the stents, to evaluate the exposed area of the stent struts, and the percentage of endothelialization through optical microscopy and scanning electron microscopy. RESULTS: The eight stents implanted with ultrasound sizing and post-dilation with a larger diameter balloon were analyzed by Doppler ultrasound, intravascular ultrasound, and angiography after 28 days. CONCLUSIONS: This study showed complete endothelial coverage by the endoluminal neointima of the stent struts, good integration and coverage with the arterial wall, with no exposed struts showing the presence of intimal hyperplasia (whitish tissue).
Assuntos
Stents Farmacológicos , Sirolimo , Animais , Sirolimo/administração & dosagem , Sirolimo/farmacologia , Suínos , Artérias Carótidas/cirurgia , Artérias Carótidas/efeitos dos fármacos , Neointima/patologia , Microscopia Eletrônica de Varredura , Poliuretanos , Estudos Prospectivos , Endotélio Vascular/efeitos dos fármacos , Reprodutibilidade dos Testes , Polímeros , Modelos Animais , Fatores de Tempo , Materiais Revestidos BiocompatíveisRESUMO
Implant-related infections pose significant challenges to orthopedic surgeries due to the high risk of severe complications. The widespread use of bioactive prostheses in joint replacements, featuring roughened surfaces and tight integration with the bone marrow cavity, has facilitated bacterial proliferation and complicated treatment. Developing antibacterial coatings for orthopedic implants has been a key research focus in recent years to address this critical issue. Researchers have designed coatings using various materials and antibacterial strategies. In this study, we fabricated 3D-printed porous titanium rods, incorporated vancomycin-loaded mPEG750-b-PCL2500 gel, and coated them with a PCL layer. We then evaluated the antibacterial efficacy through both in vitro and in vivo experiments. Our coating passively inhibits bacterial biofilm formation and actively controls antibiotic release in response to bacterial growth, providing a practical solution for proactive and sustained infection control. This study utilized 3D printing technology to produce porous titanium rod implants simulating bioactive joint prostheses. The porous structure of the titanium rods was used to load a thermoresponsive gel, mPEG750-b-PCL2500 (PEG: polyethylene glycol; PCL: polycaprolactone), serving as a novel drug delivery system carrying vancomycin for controlled antibiotic release. The assembly was then covered with a PCL membrane that inhibits bacterial biofilm formation early in infection and degrades when exposed to lipase solutions, mimicking enzymatic activity during bacterial infections. This setup provides infection-responsive protection and promotes drug release. We investigated the coating's controlled release, antibacterial capability, and biocompatibility through in vitro experiments. We established a Staphylococcus aureus infection model in rabbits, implanting titanium rods in the femoral medullary cavity. We evaluated the efficacy and safety of the composite coating in preventing implant-related infections using imaging, hematology, and pathology. In vitro experiments demonstrated that the PCL membrane stably protects encapsulated vancomycin during PBS immersion. The PCL membrane rapidly degraded at a lipase concentration of 0.2 mg/mL. The mPEG750-b-PCL2500 gel ensured stable and sustained vancomycin release, inhibiting bacterial growth. We investigated the antibacterial effect of the 3D-printed titanium material, coated with PCL and loaded with mPEG750-b-PCL2500 hydrogel, using a rabbit Staphylococcus aureus infection model. Imaging, hematology, and histopathology confirmed that our composite antibacterial coating exhibited excellent antibacterial effects and infection prevention, with good safety in trials. Our results indicate that the composite antibacterial coating effectively protects vancomycin in the hydrogel from premature release in the absence of bacterial infection. The outer PCL membrane inhibits bacterial growth and prevents biofilm formation. Upon contact with bacterial lipase, the PCL membrane rapidly degrades, releasing vancomycin for antibacterial action. The mPEG750-b-PCL2500 gel provides stable and sustained vancomycin release, prolonging its antibacterial effects. Our composite antibacterial coating demonstrates promising potential for clinical application.
Assuntos
Antibacterianos , Hidrogéis , Poliésteres , Impressão Tridimensional , Titânio , Vancomicina , Titânio/química , Vancomicina/farmacologia , Vancomicina/administração & dosagem , Vancomicina/química , Antibacterianos/farmacologia , Antibacterianos/administração & dosagem , Antibacterianos/química , Poliésteres/química , Animais , Hidrogéis/química , Coelhos , Staphylococcus aureus/efeitos dos fármacos , Liberação Controlada de Fármacos , Porosidade , Biofilmes/efeitos dos fármacos , Polietilenoglicóis/química , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/prevenção & controle , Sistemas de Liberação de Medicamentos/métodos , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologiaRESUMO
BACKGROUND: The long-term impact of drug-coated balloon (DCB) angioplasty for the treatment of patients with de novo coronary artery lesions remains uncertain. We aimed to assess the non-inferiority of DCB angioplasty with rescue stenting to intended drug-eluting stent (DES) deployment for patients with de novo, non-complex coronary artery lesions. METHODS: REC-CAGEFREE I was an open-label, randomised, non-inferiority trial conducted at 43 sites in China. After successful lesion pre-dilatation, patients aged 18 years or older with de novo, non-complex coronary artery disease (irrespective of target vessel diameter) and an indication for percutaneous coronary intervention were randomly assigned (1:1), via a web-based centralised system with block randomisation (block size of two, four, or six) and stratified by site, to paclitaxel-coated balloon angioplasty with the option of rescue stenting due to an unsatisfactory result (DCB group) or intended deployment of second-generation thin-strut sirolimus-eluting stents (DES group). The primary outcome was the device-oriented composite endpoint (DoCE; including cardiovascular death, target vessel myocardial infarction, and clinically and physiologically indicated target lesion revascularisation) assessed at 24 months in the intention-to-treat (ITT) population (ie, all participants randomly assigned to treatment). Non-inferiority was established if the upper limit of the one-sided 95% CI for the absolute risk difference was smaller than 2·68%. Safety was assessed in the ITT population. This study is registered with ClinicalTrials.gov, NCT04561739. It is closed to accrual and extended follow-up is ongoing. FINDINGS: Between Feb 5, 2021, and May 1, 2022, 2272 patients were randomly assigned to the DCB group (1133 [50%]) or the DES group (1139 [50%]). Median age at the time of randomisation was 62 years (IQR 54-69), 1574 (69·3%) of 2272 were male, 698 (30·7%) were female, and all patients were of Chinese ethnicity. 106 (9·4%) of 1133 patients in the DCB group received rescue DES after unsatisfactory DCB angioplasty. As of data cutoff (May 1, 2024), median follow-up was 734 days (IQR 731-739). At 24 months, the DoCE occurred in 72 (6·4%) of 1133 patients in the DCB group and 38 (3·4%) of 1139 in the DES group, with a risk difference of 3·04% in the cumulative event rate (upper boundary of the one-sided 95% CI 4·52; pnon-inferiority=0·65; two-sided 95% CI 1·27-4·81; p=0·0008); the criterion for non-inferiority was not met. During intervention, no acute vessel closures occurred in the DCB group and one (0·1%) of 1139 patients in the DES group had acute vessel closure. Periprocedural myocardial infarction occurred in ten (0·9%) of 1133 patients in the DCB group and nine (0·8%) in the DES group. INTERPRETATION: In patients with de novo, non-complex coronary artery disease, irrespective of vessel diameter, a strategy of DCB angioplasty with rescue stenting did not achieve non-inferiority compared with the intended DES implantation in terms of the DoCE at 2 years, which indicates that DES should remain the preferred treatment for this patient population. FUNDING: Xijing Hospital and Shenqi Medical. TRANSLATION: For the Chinese translation of the abstract see Supplementary Materials section.
Assuntos
Angioplastia Coronária com Balão , Doença da Artéria Coronariana , Stents Farmacológicos , Paclitaxel , Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Angioplastia Coronária com Balão/métodos , Paclitaxel/administração & dosagem , Paclitaxel/uso terapêutico , Doença da Artéria Coronariana/terapia , Idoso , Sirolimo/uso terapêutico , Sirolimo/administração & dosagem , Resultado do Tratamento , Materiais Revestidos Biocompatíveis , China/epidemiologia , Intervenção Coronária Percutânea/métodosRESUMO
Maintaining the differentiated phenotype and function of primary hepatocytes in vitro and in vivo represents a distinct challenge. Our paper describes microcapsules comprised of a bioactive polymer and overcoated with an ultrathin film as a means of maintaining the function of entrapped hepatocytes for at least two weeks. We previously demonstrated that heparin (Hep)-based microcapsules improved the function of entrapped primary hepatocytes by capturing and releasing cell-secreted inductive signals, including hepatocyte growth factor (HGF). Further enhancement of hepatic function could be gained by loading exogenous HGF into microcapsules. In this study, we demonstrate that an ultrathin coating of tannic acid (TA) further enhances endogenous HGF signaling for entrapped hepatocytes and increases by 2-fold the rate of uptake of exogenous HGF by Hep microcapsules. Hepatocytes in overcoated microcapsules exhibited better function and hepatic gene expression than in capsules without a TA coating. Our study showcases the potential application of ultrathin coatings to modulate the bioactivity of microcapsules and may enable the use of encapsulated hepatocytes for modeling drug toxicity or treating liver diseases.
Assuntos
Cápsulas , Heparina , Hepatócitos , Hepatócitos/metabolismo , Hepatócitos/citologia , Hepatócitos/efeitos dos fármacos , Cápsulas/química , Animais , Heparina/química , Heparina/farmacologia , Taninos/química , Taninos/farmacologia , Fator de Crescimento de Hepatócito/metabolismo , Fator de Crescimento de Hepatócito/química , Fator de Crescimento de Hepatócito/farmacologia , Esferoides Celulares/efeitos dos fármacos , Esferoides Celulares/metabolismo , Humanos , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , CamundongosAssuntos
Angioplastia Coronária com Balão , Materiais Revestidos Biocompatíveis , Angiografia Coronária , Valor Preditivo dos Testes , Ultrassonografia de Intervenção , Humanos , Angioplastia Coronária com Balão/instrumentação , Angioplastia Coronária com Balão/efeitos adversos , Resultado do Tratamento , Doença da Artéria Coronariana/diagnóstico por imagem , Doença da Artéria Coronariana/terapia , Fármacos Cardiovasculares/administração & dosagem , Cateteres Cardíacos , Stents FarmacológicosAssuntos
Angioplastia Coronária com Balão , Materiais Revestidos Biocompatíveis , Angiografia Coronária , Valor Preditivo dos Testes , Ultrassonografia de Intervenção , Humanos , Angioplastia Coronária com Balão/instrumentação , Angioplastia Coronária com Balão/efeitos adversos , Resultado do Tratamento , Doença da Artéria Coronariana/diagnóstico por imagem , Doença da Artéria Coronariana/terapia , Stents Farmacológicos , Cateteres Cardíacos , Fármacos Cardiovasculares/administração & dosagem , Vasos Coronários/diagnóstico por imagemRESUMO
Background: The most problematic complication of external fixation is infection at the pin insertion site. Technology that improves the adhesion of the external fixation pin to the skin, subcutaneous tissue, and bone may prevent infection at the pin site. The purpose of this study is to formulate a calcium phosphate-fibroblast growth factor (Cp-FGF) coating on a stainless-steel external fixation pin and to verify its effectiveness in reducing infection at the pin site and its possible influence on bone fixation in animal experiments. Methods: We compared stainless-steel screws without coating (SS group; n = 32), those with a calcium phosphate coating (Cp group; n = 30), those with a Cp-FGF coating (FGF group; n = 32), and those with a Cp-FGF coating having enhanced biological activity (FGF+ group; n = 32) in male Japanese white domesticated rabbits. Screws were inserted percutaneously into the bilateral proximal tibial diaphysis of the rabbits and implanted for 4 weeks. Screws and periscrew tissue were observed postoperatively for qualitatively assessing infection. Results: Infection assessment by gross findings after 4 weeks (at screw removal) showed no significant differences between the groups. Histopathological evaluation of soft tissue infection and bone tissue infection showed no significant differences between the groups for either soft tissue or bone tissue. Since neither the FGF+ group nor the FGF group showed anti-infective effects, the biological activity of FGF is not the only determining factor. We compared SEM, XRD, coating detaching test, sustained release test, and bioassay to examine physicochemical properties among the coatings but found no sufficient differences. Conclusions: It is suggested that improving the tissue adhesion to and/or biocompatibility of pins is also important to improve the in vivo performance of Cp-FGF-coated external fixation pins.
Assuntos
Fatores de Crescimento de Fibroblastos , Aço Inoxidável , Animais , Coelhos , Masculino , Fatores de Crescimento de Fibroblastos/uso terapêutico , Fatores de Crescimento de Fibroblastos/administração & dosagem , Fatores de Crescimento de Fibroblastos/farmacologia , Pinos Ortopédicos , Materiais Revestidos Biocompatíveis , Fosfatos de Cálcio/uso terapêutico , Anti-Infecciosos/farmacologia , Anti-Infecciosos/uso terapêutico , Anti-Infecciosos/administração & dosagemRESUMO
BACKGROUND: Dental implants are increasingly favored as a therapeutic replacement option for edentulism. Titanium (Ti), due to its excellent biocompatibility and unique osseointegration properties, is commonly used in dental implants. Various surface modifications have been explored to improve osseointegration outcomes. Graphene oxide (GO) is a promising material with various applications. Chitosan, found in the exoskeleton of crustaceans and in marine algae, has several biomedical applications. Silver (Ag) is another promising antibacterial agent that increases permeability and damages the bacterial cell membrane upon binding. OBJECTIVES: The present study applied a novel implant surface coating of Ag-decorated GO and chitosan on Ti implants to promote bone formation. We further analyzed the physiochemical and antibacterial properties of this surface coating. MATERIAL AND METHODS: A solution was prepared by mixing 3 mL of 1% chitosan solution with 10 mg of Ag-GO nanoparticles (NPs). Titanium metal was heated to 70-80°C on a hotplate and the solution was applied onto Ti to obtain an adhesive surface coating. The coated implant was further analyzed for surface properties, using scanning electron microscopy (SEM), the energy dispersive X-ray (EDX) analysis, the attenuated total reflectance-Fourier transform infrared (ATR-FTIR) technique, and the biocompatibility and corrosion analyses. RESULTS: The SEM analysis revealed a homogenously spread, rough, fibrillar and porous layer of coating on the metal surface. The EDX and ATR-FTIR analyses confirmed the successful coating of the implant surface with Ag-decorated GO and chitosan layers. The cell culture assay demonstrated excellent biocompatibility of the surface coating. The corrosion analysis showed improved corrosion resistance of the developed implant surface coating. CONCLUSIONS: The various analyses of the coating showed ideal properties for improved cell attachment, differentiation and proliferation while maintaining an antimicrobial environment on the implant surface.
Assuntos
Quitosana , Materiais Revestidos Biocompatíveis , Implantes Dentários , Grafite , Teste de Materiais , Prata , Propriedades de Superfície , Titânio , Quitosana/farmacologia , Quitosana/química , Prata/farmacologia , Corrosão , Antibacterianos/farmacologia , Microscopia Eletrônica de Varredura , HumanosRESUMO
Immunoglobulin G (IgG) comprises a significant portion of the protein corona that forms on biomaterial surfaces and holds a pivotal role in modulating host immune responses. To shed light on the important relationship between biomaterial surface functionality, IgG adsorption, and innate immune responses, we prepared, using plasma deposition, four surface coatings with specific chemistries, wettability, and charge. We found that nitrogen-containing coatings such as these deposited from allylamine (AM) and 2-methyl-2-oxazoline (POX) cause the greatest IgG unfolding, while hydrophilic acrylic acid (AC) surfaces allowed for the retention of the protein structure. Structural changes in IgG significantly modulated macrophage attachment, migration, polarization, and the expression of pro- and anti-inflammatory cytokines. Unfolded IgG on the POX and AM surfaces enhanced macrophage attachment, migration, extracellular trap release, and pro-inflammatory factors production such as IL-6 and TNF-α. Retention of IgG structure on the AC surface downregulated inflammatory responses. The findings of this study demonstrate that the retention of protein structure is an essential factor that must be taken into consideration when designing biomaterial surfaces. Our study indicates that using hydrophilic surface coatings could be a promising strategy for designing immune-modulatory biomaterials for clinical applications.
Assuntos
Imunoglobulina G , Propriedades de Superfície , Imunoglobulina G/química , Imunoglobulina G/imunologia , Camundongos , Animais , Desdobramento de Proteína , Macrófagos/imunologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Humanos , Células RAW 264.7 , Interações Hidrofóbicas e Hidrofílicas , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Adsorção , Citocinas/metabolismo , Citocinas/imunologiaRESUMO
BACKGROUND: Spinal implants' longevity is crucial, but titanium alloys, while advantageous, lack strong bone integration. This study aimed to achieve better osseointegration rates by utilizing the ability of boron compounds to transform stem cells in the vertebra into osteoblasts. METHOD: Twenty male albino rabbits were divided into control (n = 10) and experimental (n = 10) groups. Control group received titanium alloy pedicle screws, while experimental group received boron-coated titanium alloy screws. Under general anesthesia, screws were inserted into the L6 and L7 lumbar spines. After 16 weeks, all animals were euthanized for histological examination. Vertebra samples underwent decalcification and H&E staining. Microscopic examination assessed osseointegration, necrosis, fibrosis, and vascularization using a triple scoring system by two blinded observers. RESULT: In the boron-coated titanium alloy group, all subjects exhibited osseointegration, with 50% showing focal, 40% moderate, and 10% complete osseointegration. In the titanium alloy group, 90% showed osseointegration (70% focal, 10% moderate, and 10% complete).The differences between the groups were not statistically significant (p = 0.302). Focal necrosis rates were similar between groups, with 50.0% in the titanium alloy and 60.0% in the boron-coated group (p = 0.653).Fibrosis was absent in the titanium alloy group but present in the boron-coated group, albeit with lower rates of focal fibrosis (20.0%). However, the difference was not statistically significant (p = 0.086).Vascularization patterns showed no significant difference between groups. CONCLUSION: Boron-coated titanium alloy pedicle screws provided osseointegration rates comparable to standard titanium screws and exhibited acceptable levels of necrosis and fibrosis. With stronger biomechanical properties, they could be a better alternative to currently used titanium screws.
Assuntos
Ligas , Boro , Osseointegração , Parafusos Pediculares , Titânio , Animais , Osseointegração/efeitos dos fármacos , Coelhos , Masculino , Boro/farmacologia , Boro/química , Materiais Revestidos Biocompatíveis , Vértebras Lombares/cirurgiaRESUMO
In recent years, with the advent of a super-aged society, lifelong dental care has gained increasing emphasis, and implant therapy for patients with an edentulous jaw has become a significant option. However, for implant therapy to be suitable for elderly patients with reduced regenerative and immunological capabilities, higher osteoconductive and antimicrobial properties are required on the implant surfaces. Silicon nitride, a non-oxide ceramic known for its excellent mechanical properties and biocompatibility, has demonstrated high potential for inducing hard tissue differentiation and exhibiting antibacterial properties. In this study, silicon nitride was deposited on pure titanium metal surfaces and evaluated for its biocompatibility and antibacterial properties. The findings indicate that silicon nitride improves the hydrophilicity of the material surface, enhancing the initial adhesion of rat bone marrow cells and promoting hard tissue differentiation. Additionally, the antibacterial properties were assessed using Staphylococcus aureus, revealing that the silicon nitride-coated surfaces exhibited significant antibacterial activity. Importantly, no cytotoxicity was observed, suggesting that silicon nitride-coated titanium could serve as a novel implant material.
Assuntos
Antibacterianos , Materiais Revestidos Biocompatíveis , Compostos de Silício , Staphylococcus aureus , Propriedades de Superfície , Titânio , Titânio/química , Titânio/farmacologia , Animais , Antibacterianos/farmacologia , Antibacterianos/química , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Ratos , Staphylococcus aureus/efeitos dos fármacos , Compostos de Silício/química , Compostos de Silício/farmacologia , Teste de Materiais , Adesão Celular/efeitos dos fármacos , Células da Medula Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacosRESUMO
Purpose: The paper shows a preliminary study of the basic strength parameters of printed parts made of biocompatible polymers with ceramic layers applied to increase the strength of the tool cutting surface. Methods: The specimens were made from different materials and using different 3D printing technologies and the working surfaces that will eventually form the cutting element of the tool were coated with Al2O3. Gloss tests were conducted, properties of the coating, a scratch test of the coated surface, also evaluated surface to-pography. Results: Based on the conducted research, it was found that polymeric materials are characterized by sufficient strength and can be used for disposable tools, however, the use of thin layers of Al2O3 significantly increases the surface strength parameters, which may have a significant impact on the reliability and durability of the tools. The polymer surface covered with an Al2O3 layer is characterised by increased scratch resistance ranging from 24% to 75% depending on the core material and printing technology. The gloss of the surfaces is disproportionately low compared to currently used metal tools, which indicates that they can be used in endoscopic procedures. Conclusions: Based on the conducted research, it was found that the use of thin layers of Al2O3 covering polymer 3D prints is an excellent way to increase strength parameters such as scratch resistance, tribological parameters and light reflections arising on the surface as a result of endoscopic lighting are disproportionately small compared to metallic biomaterials. This gives great hope for using polymer 3D prints for personalised neurosurgical tools.
Assuntos
Cerâmica , Teste de Materiais , Impressão Tridimensional , Cerâmica/química , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Propriedades de Superfície , Óxido de Alumínio/químicaRESUMO
Purpose: Titanium alloys are among the most widely used materials in medicine, especially in orthopedics. However, their use requires the application of an appropriate surface modification method to improve their properties. Such methods include anodic oxidation and the application of polymer coatings, which limit the release of alloying element ions. In addition, biodegradable polymer coatings can serve as a carrier for drugs and other substances. The paper presents the results of research on the physical properties of biodegradable polymer coatings containing nanoparticle hydroxyapatite on a titanium alloy substrate. Methods: A PLGA coating was used in the tests. The coatings on the substrate of the anodized Ti6Al7Nb alloy were applied by ultrasonic spray coating. The tests were carried out for coatings with various hydroxyapatite content (5, 10, 15, 20%) and thickness resulting from the number of layers applied (5, 10, 15 layers). The scope of the research included microscopic observations using scanning electron microscopy, topography tests with optical profilometry, structural studies using X-ray diffraction, as well as wettability and adhesion tests. Results: The results shows that with the use of ultrasonic spray coating system is possible to obtain the continuous coatings containing hydroxyapaptite. Conclusions: The properties of the coating can be controlled by changing the percentage of hydroxyapatite and the number of layers of which the coating is composed.
Assuntos
Ligas , Materiais Revestidos Biocompatíveis , Durapatita , Titânio , Durapatita/química , Materiais Revestidos Biocompatíveis/química , Titânio/química , Ligas/química , Teste de Materiais , Difração de Raios X , Molhabilidade , Polímeros/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Propriedades de SuperfícieAssuntos
Angioplastia Coronária com Balão , Humanos , Angioplastia Coronária com Balão/instrumentação , Angioplastia Coronária com Balão/efeitos adversos , Angioplastia Coronária com Balão/métodos , Stents Farmacológicos , Resultado do Tratamento , Vasos Coronários/diagnóstico por imagem , Doença da Artéria Coronariana/terapia , Materiais Revestidos BiocompatíveisRESUMO
Chitosan is a promising natural polymer for coatings, it combines intrinsic antibacterial and pro-osteoblastic properties, but the literature still has a gap from the development to behavior of these coatings, so this systematic review aimed to answer, "What is the relationship between the physical and chemical properties of polymeric chitosan coatings on titanium implants on antibacterial activity and osteoblast viability?". PRISMA guidelines was followed, the search was applied into 4 databases and grey literature, without the restriction of time and language. The selection process occurred in 2 blinded steps by the authors. The criteria of eligibility were in vitro studies that evaluated the physical, chemical, microbiological, and biological properties of chitosan coatings on titanium surfaces. The risk of bias was analyzed by the specific tool. Of 734 potential articles 10 were included; all had low risk of bias. The coating was assessed according to the technique of fabrication, FT-IR, thickness, adhesion, roughness, wettability, antibacterial activity, and osteoblast viability. The analyzed coatings showed efficacy on antibacterial activity and cytocompatibility dependent on the class of material incorporated. Thus, this review motivates the development of time-dependent studies to optimize manufacturing and allow for an increase in patents and availability on the market.
Assuntos
Antibacterianos , Quitosana , Materiais Revestidos Biocompatíveis , Osteoblastos , Titânio , Quitosana/química , Quitosana/farmacologia , Titânio/química , Titânio/farmacologia , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Humanos , Antibacterianos/farmacologia , Antibacterianos/química , Osteoblastos/efeitos dos fármacos , Osteoblastos/citologia , Propriedades de Superfície , Próteses e Implantes , Animais , Sobrevivência Celular/efeitos dos fármacosRESUMO
Graphitic carbon nitride (g-C3N4, CN) has emerged as a promising photocatalytic material due to its inherent stability, antibacterial properties, and eco-friendliness. However, its tendency to aggregate and limited dispersion hinder its efficacy in practical antibacterial applications. To address these limitations, this study focuses on developing a composite hydrogel coating, in which sodium alginate (SA) molecules interact electrostatically and through hydrogen bonding to anchor CN, thereby significantly improving its dispersion. The optimal CN loading of 35% results in a hydrogel with a tensile strength of 120 MPa and an antibacterial rate of 99.87% within 6 h. The enhanced mechanical properties are attributed to hydrogen bonding between the -NH2 groups of CN and the -OH groups of SA, while the -OH groups of SA facilitate the attraction of photogenerated holes from CN, promoting carrier transfer and separation, thereby strengthening the antibacterial action. Moreover, the hydrogel coating exhibits excellent antibacterial and corrosion resistance capabilities against Pseudomonas aeruginosa on 316L stainless steel (316L SS), laying the foundation for advanced antimicrobial and anticorrosion hydrogel systems.