RESUMO
The aim of this study was to investigate the effects of Tris use supplemented with Mauritia Flexuoxa oilas diluent for goat semen cryopreservation. four goats, clinically healthy were used. The animals were fed dailywith roughage, concentrate and mineral salt will. Initially, we conducted a toxicity test total of 32 collections.After the toxicity test, the concentration was chosen that showed the best result. Soon after, there were 32 morecollections, which were diluted in Tris-egg yolk-glycerol (control group) or GC diluent containing vegetable oil(Mauritia flexuoxa) GB. Samples were cryopreserved with the aid of Tk3000® device. Subsequently sampleswere thawed and analyzed for motility and sperm morphology force. It was observed obtaining post thawmotility in the group plus oil burity which shows a possible alternative to replacement products derived fromanimal origin.
Assuntos
Masculino , Animais , Análise do Sêmen/métodos , Análise do Sêmen/veterinária , Criopreservação/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/fisiologiaRESUMO
The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.
Assuntos
Masculino , Animais , Análise do Sêmen/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/química , RuminantesRESUMO
The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.(AU)
Assuntos
Animais , Masculino , Análise do Sêmen/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/química , RuminantesRESUMO
The aim of this study was to investigate the effects of Tris use supplemented with Mauritia Flexuoxa oilas diluent for goat semen cryopreservation. four goats, clinically healthy were used. The animals were fed dailywith roughage, concentrate and mineral salt will. Initially, we conducted a toxicity test total of 32 collections.After the toxicity test, the concentration was chosen that showed the best result. Soon after, there were 32 morecollections, which were diluted in Tris-egg yolk-glycerol (control group) or GC diluent containing vegetable oil(Mauritia flexuoxa) GB. Samples were cryopreserved with the aid of Tk3000® device. Subsequently sampleswere thawed and analyzed for motility and sperm morphology force. It was observed obtaining post thawmotility in the group plus oil burity which shows a possible alternative to replacement products derived fromanimal origin.(AU)
Assuntos
Animais , Masculino , Criopreservação/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/fisiologia , Análise do Sêmen/métodos , Análise do Sêmen/veterináriaRESUMO
UNLABELLED: "Matè", a popular hot infusion of a herb (Ilex paraguayensis) drunk in large volumes, is a known risk factor for squamous cell carcinoma of the esophagus and there is a suspicion that high temperature of boiled water used for the infusion may contribute for carcinogenesis. METHODS: We measured the temperature of "matè" infusion drunk by a sample of the population at risk for this carcinoma in Taquara, southern Brazil. We interviewed inhabitants for drinking habits and the temperature of the infusion was measured with high precision thermometers. Temperature of the infusion was asked to consumers and their estimate compared to our measurements. We considered 60 degrees C or higher as "hot". RESULTS: In 36 residencies, 107 individuals were drinking "matè". Most individuals drank it daily (97.2%), and the medium daily volume was 1,265 ml (SD +/- 1,132 mL) ranging from 250 to 6,000 mL. The measured temperature was 60 degrees C or higher in 72% of residencies with medium of 63.4 degrees C (51-78 degrees C) and median 64.4 degrees C. CONCLUSION: In this study, "matè" was consumed in large volumes at high temperature and individuals did not estimate correctly the temperature of infusion. High temperatures of "matè" may contribute to carcinogenesis in this population.
Assuntos
Bebidas/efeitos adversos , Carcinoma de Células Escamosas/etiologia , Neoplasias Esofágicas/etiologia , Comportamento Alimentar , Temperatura Alta/efeitos adversos , Adulto , Idoso , Brasil , Feminino , Humanos , Magnoliopsida/efeitos adversos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Estatísticas não Paramétricas , Inquéritos e Questionários , Sensação Térmica , População UrbanaRESUMO
During the period from January 1988 to December 1994, a case-control study that included 497 cases of lung cancer and 497 controls was carried out at the Instituto de Oncologia, Montevideo, Uruguay, to evaluate the relationship between the drinking of mate (a local tea prepared with infusions of the herb Ilex paraguariensis) and the risk of lung cancer in men. Mate drinking has been associated with risk of most upper-aerodigestive tract cancers. After adjusting for major covariates, including pack-years of cigarette smoking, the amount of mate was associated with a 1.6-fold increase in risk for heavy drinkers, compared with light drinkers, with a significant dose-response pattern. When the analysis was performed by cell type, small cell lung cancer showed a significant increase in relative risk for mate amount (odds ratio, 2.9; 95% confidence interval, 1.3-6.2) and mate duration (odds ratio, 3.6; 95% confidence interval, 1.3-9.9). On the other hand, pulmonary adenocarcinoma was not associated with mate drinking. Possible reasons for these results are discussed, and areas for future research are suggested.
Assuntos
Bebidas/efeitos adversos , Neoplasias Pulmonares/epidemiologia , Magnoliopsida/efeitos adversos , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Intervalos de Confiança , Humanos , Incidência , Neoplasias Pulmonares/diagnóstico , Masculino , Pessoa de Meia-Idade , Razão de Chances , Valores de Referência , Fatores de Risco , Fumar/efeitos adversos , Uruguai/epidemiologiaRESUMO
A hospital-based case-control study, including 131 cases of esophageal cancer and 381 controls, was carried out in Paraguay to investigate the role of hot and cold mate drinking in esophageal cancer risk. Detailed information on mate drinking and on tobacco smoking, alcohol consumption, and dietary habits was obtained by interview. Amount and duration of cold or hot mate drinking were not associated with esophageal cancer risk. However, temperature at which mate was drunk was significantly associated with risk. As compared to drinkers of warm or hot mate, drinkers of very hot mate had an increased risk for esophageal cancer even after adjusting for the strong effects of alcohol and tobacco consumption (adjusted odds ratio = 2.4; 95% confidence interval = 1.3-4.3). This effect seemed to be mainly due to the temperature at which mate cocido (one of the two ways in which hot mate is prepared) was drunk (odds ratio = 6.5; 95% confidence interval = 3.2-12.2). As expected, very strong dose-response associations were found for alcohol consumption and cigarette smoking. After correcting for these and the consumption of other food groups, diets rich in fats and red meats, especially beef, were associated with esophageal cancer risk. In conclusion, the findings from this study suggest that cold mate drinking does not increase the risk of esophageal cancer. This study identifies the very hot temperature at which mate is drunk, and not the amount or the duration, as an important risk factor for esophageal cancer in this population. Alcohol drinking and tobacco smoking remain, nevertheless, the main risk factors for esophageal cancer in Paraguay.
Assuntos
Bebidas/efeitos adversos , Neoplasias Esofágicas/etiologia , Comportamento Alimentar , Magnoliopsida/efeitos adversos , Adulto , Distribuição por Idade , Idoso , Consumo de Bebidas Alcoólicas/efeitos adversos , Estudos de Casos e Controles , Temperatura Baixa , Intervalos de Confiança , Neoplasias Esofágicas/epidemiologia , Feminino , Temperatura Alta , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Razão de Chances , Paraguai/epidemiologia , Análise de Regressão , Fatores de Risco , Distribuição por Sexo , Fumar/efeitos adversosRESUMO
Consumption of maté, a tea-like infusion of the herb Ilex paraguariensis, is common in South America. Drinkers have high risks of upper aerodigestive tract cancers, but it is conceivable that this high risk may be attributable to confounding by smoking alcohol, and other exposures. To test this hypothesis, we analyzed the data from a case-control study of upper aerodigestive tract cancers conducted in Southern Brazil. We matched noncancer controls (N = 756) to cases (N = 378) on the basis of age, sex, and period of admission. We estimated the effect of mateé consumption by conditional logistic regression with adjustment for smoking, alcohol, sociodemographics, and several dietary items, considered as confounders. The unadjusted relative risk (RR) for all upper aerodigestive tract cancers was 2.1 [95% confidence interval (CI) = 1.6-2.7]. Some excess risk persisted after adjustment for potential confounders (RR = 1.6; 95% CI = 1.2-2.2). Most of the excess risk for maté drinkers was for oral (RR = 1.9; 95% CI = 1.1-3.3) and laryngeal (RR = 2.2; 95% CI = 2.2; 95% CI = 1.1-4.5) cancers. There was no evidence of associations with coffee and tea drinking. We conclude that the association of maté consumption with upper aerodigestive tract cancer risk is unlikely to result from insufficient control of confounding by critical exposures. Owing to its high prevalence in Southern South America, maté drinking may be linked to as many as 20% of all cases occurring in this region.