RESUMO
In the last years, due to the high demand of food products supplemented with probiotics and the multiple nutritional and therapeutic benefits associated with them, research on these microorganisms has advanced considerably, including their selection and characterization. As a general recommendation, several entities as World Health Organization (WHO) and United Nations Organization for Agriculture and Food recommend that the specification of the alive species contained and their number shall appear in the label of the product. In the present study, six different commercially available products, supplemented with probiotics were analyzed, in order to evaluate the concentration of microorganisms through the shelf life of the product, identify the strains isolated and determine the antibiotic susceptibility pattern of these. Results demonstrated that the strains isolated kept acceptable concentrations during the 28 days of storage. Nevertheless, the identification of these strains variated from the one reported on the label on several of the products tested. This can be due to the commercial method used for the identifications, which is based in the carbohydrate fermentation pattern and not in genotypic trials. The antimicrobials' susceptibility patterns found show that further research shall be performed in order to establish the intrinsic or acquired nature of the resistance determinants, and if these are codified by transferable elements among bacteria.
Assuntos
Antibacterianos/farmacologia , Laticínios/microbiologia , Lactobacillus/efeitos dos fármacos , Leuconostoc/efeitos dos fármacos , Probióticos , Streptococcus thermophilus/efeitos dos fármacos , Costa Rica , Lactobacillus/isolamento & purificação , Leuconostoc/isolamento & purificação , Testes de Sensibilidade Microbiana , Probióticos/isolamento & purificação , Streptococcus thermophilus/isolamento & purificaçãoRESUMO
The species of Leuconostoc are uncommon opportunistic pathogens, which can be isolated in critically ill patients, immunocompromised hosts and in nosocomial infections. They are mostly isolated in bacteremia associated to intravascular devices and to the use of total parenteral nutrition. Nevertheless, other infections due to Leuconostoc sp have been described among which, meningitis, bloodstream infections, urinary tract infections and peritonitis have been reported. In this article we describe a series of cancer patients with Leuconostoc sp infections and their associated clinical conditions, and a literature review is presented.
Assuntos
Infecção Hospitalar/microbiologia , Infecções por Bactérias Gram-Positivas/microbiologia , Leuconostoc/isolamento & purificação , Neoplasias/microbiologia , Adulto , Idoso , Antibacterianos/uso terapêutico , Criança , Feminino , Infecções por Bactérias Gram-Positivas/diagnóstico , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Humanos , Leuconostoc/efeitos dos fármacos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Estudos RetrospectivosRESUMO
Las especies de Leuconostoc son patógenas oportunistas informadas en muy baja frecuencia, que se pueden encontrar en pacientes críticamente enfermos, inmunocomprometidos y con infecciones intra-hospitalarias. Generalmente, se asocian a bacteriemia por dispositivos intra-vasculares y al uso de nutrición parenteral total. Sin embargo, también se han descrito otras infecciones asociadas, dentro de las que se cuentan meningitis, osteomielitis, infección del torrente sanguíneo, de vías urinarias y peritonitis. En este artículo se describe una serie de pacientes con cáncer en quienes se identificó este microorganismo, las condiciones clínicas asociadas y se hace una revisión de la literatura.
The species of Leuconostoc are uncommon opportunistic pathogens, which can be isolated in critically ill patients, immunocompromised hosts and in nosocomial infections. They are mostly isolated in bacteremia associated to intravascular devices and to the use of total parenteral nutrition. Nevertheless, other infections due to Leuconostoc sp have been described among which, meningitis, bloodstream infections, urinary tract infections and peritonitis have been reported. In this article we describe a series of cancer patients with Leuconostoc sp infections and their associated clinical conditions, and a literature review is presented.
Assuntos
Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Infecção Hospitalar/microbiologia , Infecções por Bactérias Gram-Positivas/microbiologia , Leuconostoc/isolamento & purificação , Neoplasias/microbiologia , Antibacterianos/uso terapêutico , Infecções por Bactérias Gram-Positivas/diagnóstico , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Leuconostoc/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estudos RetrospectivosRESUMO
The phenotypic and genotypic characterization of five clinical isolates of Leuconostoc pseudomesenteroides associated with nosocomially acquired urinary tract infections is described. All the strains were susceptible to chloramphenicol, clindamycin, erythromycin, gentamicin, and tetracycline; all were resistant to nalidixic acid, norfloxacin, and vancomycin; and all were intermediately affected by ampicillin and penicillin. Analysis of chromosomal DNA by pulsed-field gel electrophoresis after treatment with SmaI indicated a clonal relationship of the isolates. The results provide evidence for the possibility of nosocomial transmission of this unusual opportunistic, vancomycin-resistant pathogen.
Assuntos
Infecção Hospitalar/microbiologia , Infecções por Bactérias Gram-Positivas/microbiologia , Leuconostoc/classificação , Infecções Urinárias/microbiologia , Adolescente , Adulto , Técnicas de Tipagem Bacteriana , Infecção Hospitalar/transmissão , Eletroforese em Gel de Campo Pulsado , Eletroforese em Gel de Poliacrilamida , Feminino , Infecções por Bactérias Gram-Positivas/transmissão , Humanos , Leuconostoc/efeitos dos fármacos , Leuconostoc/genética , Leuconostoc/isolamento & purificação , Masculino , Testes de Sensibilidade Microbiana , Infecções Urinárias/transmissãoRESUMO
Among three strains of "Pycnosporus sanguineus", MIP 89007 produced more cinnabarin than MIP 95001 and MIP 95002. The antimicrobial activity of cinnabarin was tesred against 11 species of bacteria isolated from food. "Bacillus cereus" and "Leuconostoc plantarum" were the most sensitive to cinnabarin, being inhibited by 0.0625 mg/ml. "Klebsiella pneumonia" was the least sensitive (>4.0 mg/ml).
Assuntos
Polyporaceae/metabolismo , Catalase/biossíntese , Técnicas In Vitro , Bacillus cereus/efeitos dos fármacos , Catalase/farmacologia , Testes de Sensibilidade Microbiana , Microbiologia de Alimentos , Leuconostoc/efeitos dos fármacosRESUMO
Twenty strains of Pediococcus pentosaceus isolated from wine were examined for production of bacteriocins. Only two of them showed inhibitory activity, Ped. pentosaceus N4p against the indicator strains of the same species and N5p against 19 strains of the three genera of lactic acid bacteria from wine. The antimicrobial substance from N5p strains was removed by membrane (0.2 micron) filtration, destroyed by organic solvents and proteolytic enzymes. It was stable for 60 min at 100 degrees C. The bacteriocin was produced early in the growth cycle and its production was maximum after 48 h of culture in tomato juice medium at an initial pH of 6.5. The bactericidal effect was observed.