RESUMO
The authors have previously studied the presence and distribution of a 24-kilodalton (KD) estrogen-regulated protein in the human normal cervix (Am J Obstet Gynecol 1986; 155:1090-1096). This protein has recently been identified as a heat-shock protein, and in order to continue its study the authors have now examined its expression in preneoplastic to neoplastic cervical samples. The study involved 53 patients, the presence of 24-KD protein together with keratin and carcinoembryonic antigen (CEA) was investigated by immunohistochemical analysis. Cytosol samples from 15 patients with squamous cervical carcinomas were also studied by the Western blot technique, and the presence of estrogen receptors was analyzed biochemically. The 24-KD protein was observed in cervical intraepithelial neoplasias (CIN), but it was not useful to identify the different degrees of CIN examined. The 24-KD protein, keratin, and CEA were predominantly expressed in well and moderately differentiated squamous carcinomas in the more differentiated areas, and the protein was also found in cervical adenocarcinomas. The presence of 24-KD protein did not correlate with that of estrogen receptors in squamous cervical carcinomas. The Western blot and the immunohistochemical studies revealed that the antibody to 24-KD protein does not cross-react with epitopes of CEA and keratins.
Assuntos
Colo do Útero/análise , Proteínas de Choque Térmico/análise , Lesões Pré-Cancerosas/análise , Neoplasias do Colo do Útero/análise , Adenocarcinoma/análise , Western Blotting , Antígeno Carcinoembrionário/análise , Carcinoma de Células Escamosas/análise , Feminino , Humanos , Imuno-Histoquímica , Queratinas/análise , Peso Molecular , Receptores de Estrogênio/análiseRESUMO
The gastric juice of patients with atrophic gastritis from the high-risk gastric cancer region of Nariño, Colombia, was analyzed for nitrate, nitrite, and thiocyanate content. Patients whose gastric juice pH is greater than 5 have significant elevation of nitrite values. The possible role of nitrite in gastric carcinogenesis is discussed.
Assuntos
Suco Gástrico/análise , Lesões Pré-Cancerosas/análise , Neoplasias Gástricas/análise , Adulto , Idoso , Colômbia , Feminino , Gastrite/complicações , Humanos , Concentração de Íons de Hidrogênio , Masculino , Pessoa de Meia-Idade , Nitratos/análise , Nitritos/análise , Risco , Neoplasias Gástricas/epidemiologia , Neoplasias Gástricas/etiologiaRESUMO
Samples of gastric contents from 2 groups of patients from a region of high risk for gastric cancer were analyzed for pH, nitrite, nitrate, thiocyanate, and chloride. In each group, the patients could be divided into 2 subgroups: those with a gastric pH of less than 5 and those with a gastric pH of greater than 5. Above pH 5, nitrite was correlated with nitrate. The pH greater than 5 subgroups had significantly higher (P less than 0.01) nitrite content (20- to 100-fold). Some high- and low-nitrite samples were also analyzed for macro and trace metal ions, but differences were not significant. This is the first report in which patients with diagnosed gastric pathology related to a precancerous state were shown to have high levels of a putative carcinogen precursor. The results were compatible with our original hypothesis of intragastric nitrite formation by bacterial reduction of nitrate and concomitant synthesis of carcinogenic N-nitroso compounds.