RESUMO
Lectins are proteins or glycoproteins of non-immunological origin capable of reversibly and specifically binding to glycoconjugates. They exist in free form or associated with cells and are widely distributed in nature, being found in plants, microorganisms, and animals. Due to their characteristics and mainly due to the possibility of reversible binding to glycoconjugates, lectins have stood out as important tools in research involving Neurobiology. These proteins have the ability to modulate molecular targets in the central nervous system (CNS) which may be involved with neuroplasticity, neurobehavioral effects, and neuroprotection. The present report integrates existing information on the activity of animal and plant lectins in different areas of Neuroscience, presenting perspectives to direct new research on lectin function in the CNS, providing alternatives for understanding neurological diseases such as mental disorders, neurodegenerative, and neuro-oncological diseases, and for the development of new drugs, diagnoses and therapies in the field of Neuroscience.
Assuntos
Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Galectinas/administração & dosagem , Galectinas/metabolismo , Lectinas de Plantas/administração & dosagem , Lectinas de Plantas/metabolismo , Animais , Humanos , Plasticidade Neuronal/efeitos dos fármacos , Fármacos Neuroprotetores/administração & dosagem , Neurociências , Projetos de PesquisaRESUMO
BACKGROUND: Lectins, carbohydrate-binding proteins, from the bark (MuBL) and leaf (MuLL) of Myracrodruon urundeuva are termiticidal agents against Nasutitermes corniger workers and have been shown to induce oxidative stress and cell death in the midgut of these insects. In this study, we investigated the binding targets of MuBL and MuLL in the gut of N. corniger workers by determining the effects of these lectins on the activity of digestive enzymes. In addition, we used mass spectrometry to identify peptides from gut proteins that adsorbed to MuBL-Sepharose and MuLL-Sepharose columns. RESULTS: Exoglucanase activity was neutralized in the presence of MuBL and stimulated by MuLL. α-l-Arabinofuranosidase activity was not affected by MuBL but was inhibited by MuLL. Both lectins stimulated α-amylase activity and inhibited protease and trypsin-like activities. Peptides with homology to apolipophorin, trypsin-like enzyme, and ABC transporter substrate-binding protein were detected from proteins that adsorbed to MuBL-Sepharose, while peptides from proteins that bound to MuLL-Sepharose shared homology with apolipophorin. CONCLUSION: This study revealed that digestive enzymes and transport proteins found in worker guts can be recognized by MuBL and MuLL. Thus, the mechanism of their termiticidal activity may involve changes in the digestion and absorption of nutrients. © 2018 Society of Chemical Industry.
Assuntos
Anacardiaceae/química , Inseticidas/metabolismo , Isópteros/efeitos dos fármacos , Lectinas de Plantas/metabolismo , Animais , Sistema Digestório/efeitos dos fármacos , Sistema Digestório/enzimologia , Isópteros/enzimologia , Casca de Planta/química , Folhas de Planta/química , Lectinas de Plantas/administração & dosagemRESUMO
Tumor-associated macrophages (TAMs) have an ambiguous and complex role in the carcinogenic process, since these cells can be polarized into different phenotypes (proinflammatory, antitumor cells or anti-inflammatory, protumor cells) by the tumor microenvironment. Given that the interactions between tumor cells and TAMs involve several players, a better understanding of the function and regulation of TAMs is crucial to interfere with their differentiation in attempts to skew TAM polarization into cells with a proinflammatory antitumor phenotype. In this study, we investigated the modulation of macrophage tumoricidal activities by the lectin jacalin. Jacalin bound to macrophage surface and induced the expression and/or release of mainly proinflammatory cytokines via NF-κB signaling, as well as increased iNOS mRNA expression, suggesting that the lectin polarizes macrophages toward the antitumor phenotype. Therefore, tumoricidal activities of jacalin-stimulated macrophages were evaluated. High rates of tumor cell (human colon, HT-29, and breast, MCF-7, cells) apoptosis were observed upon incubation with supernatants from jacalin-stimulated macrophages. Taken together, these results indicate that jacalin, by exerting a proinflammatory activity, can direct macrophages to an antitumor phenotype. Deep knowledge of the regulation of TAM functions is essential for the development of innovative anticancer strategies.
Assuntos
Neoplasias da Mama/tratamento farmacológico , Neoplasias do Colo/tratamento farmacológico , Macrófagos/efeitos dos fármacos , Lectinas de Plantas/administração & dosagem , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Neoplasias do Colo/genética , Neoplasias do Colo/patologia , Feminino , Regulação Neoplásica da Expressão Gênica , Células HT29 , Humanos , Células MCF-7 , Macrófagos/metabolismo , NF-kappa B/genética , Óxido Nítrico Sintase Tipo II/biossíntese , Óxido Nítrico Sintase Tipo II/genética , RNA Mensageiro/biossíntese , Transdução de Sinais/efeitos dos fármacos , Microambiente Tumoral/efeitos dos fármacos , Microambiente Tumoral/genéticaRESUMO
Andira anthelmia (tribe Dalbergieae), a plant from Brazilian Amazon, possesses a seed lectin that was purified by affinity chromatography in sepharose-mannose. This novel Dalbergieae lectin, named AAL, agglutinated rabbit erythrocytes treated with trypsin. The hemagglutinating activity of AAL was maintained after incubation at a wide range of temperature (40 to 70 °C) and pH, was shown to be dependent on divalent cations, and was inhibited by d-mannose and d-sucrose. AAL showed an electrophoretic profile in sodium dodecyl sulfate-polyacrylamide gel electrophoresis similar to other lectins of the tribe Dalbergieae, presenting a double band of molecular weight with approximately 20 kDa and other minor bands of 17, 15, and 13 kDa, being the smaller fragment glycosylated. AAL injected by intravenous route in mice showed antinociceptive activity in two behavioral tests (writhing and formalin). In the writhing test induced by acetic acid, AAL showed inhibitory effect at 0.01 mg/kg (68%), 0.1 mg/kg (46%) and 1 mg/kg (74%). In the formalin test, AAL (0.1 mg/kg) inhibited by 48% the licking time in the inflammatory phase, an effect that was recovered by the lectin association with mannose. In conclusion, AAL presents analgesic effect involving the lectin domain via peripheral mechanisms of inflammatory nociception. This activity highlights the importance of lectins as tools to be used for understanding the interaction of protein-carbohydrate in processes associated to inflammatory pain. Copyright © 2015 John Wiley & Sons, Ltd.
Assuntos
Analgésicos/administração & dosagem , Analgésicos/isolamento & purificação , Fabaceae/química , Dor/tratamento farmacológico , Lectinas de Plantas/administração & dosagem , Lectinas de Plantas/isolamento & purificação , Analgésicos/química , Analgésicos/farmacologia , Animais , Cromatografia de Afinidade , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Eritrócitos/efeitos dos fármacos , Testes de Hemaglutinação , Concentração de Íons de Hidrogênio , Manose/farmacologia , Camundongos , Peso Molecular , Dor/etiologia , Lectinas de Plantas/química , Lectinas de Plantas/farmacologia , Estabilidade Proteica , Coelhos , Sacarose/farmacologia , TemperaturaRESUMO
Silver nanoparticles (AgNPs) induce diverse cell-death mechanisms, similar to those promoted by anticancer chemotherapeutics; however, they have not been tested in vivo because their action is not limited to cancer cells. Therefore, in vivo evaluations of their effectiveness should be developed with targeting systems. Breast cancer shows changes in the sugar expression patterns on cell surfaces, related to cancer progression and metastases; those changes have been identified previously by the specific binding of soybean agglutinin (SBA). Here is proposed the use of SBA to target the AgNP activity in breast cancer. For that, the present work reports the synthesis of AgNPs (3.89 ± 0.90 nm) through the polyol method, the generation of AgNP nanocarriers, and the bioconjugation protocol of the nanocarrier with SBA. The free AgNPs, the AgNP nanocarriers, and the SBA-bioconjugated AgNP nanocarriers were tested for cytotoxicity in breast cancerous (MDA-MB-231and MCF7) and non cancerous (MCF 10A) cells, using the MTT assay. AgNPs demonstrated cytotoxic activity in vitro, the non cancerous cells (MCF 10A) being more sensible than the cancerous cells (MDA-MB-231 and MCF7) showing LD(50) values of 128, 205, and 319 µM Ag, respectively; the nanoencapsulation decreased the cytotoxic effect of AgNPs in non cancerous cells, maintaining or increasing the effect on the cancer-derived cells, whereas the SBA-bioconjugation allowed AgNP cytotoxic activity with a similar behavior to the nanocarriers. Future experiments need to be developed to evaluate the targeting effect of the SBA-bioconjugated AgNP nanocarriers to study their functionality in vivo.
Assuntos
Antineoplásicos/administração & dosagem , Neoplasias da Mama/tratamento farmacológico , Portadores de Fármacos , Nanopartículas Metálicas , Lectinas de Plantas/administração & dosagem , Compostos de Prata , Proteínas de Soja/administração & dosagem , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Portadores de Fármacos/síntese química , Portadores de Fármacos/química , Difusão Dinâmica da Luz , Humanos , Hidrodinâmica , Concentração de Íons de Hidrogênio , Nanopartículas Metálicas/administração & dosagem , Nanopartículas Metálicas/química , Microscopia Eletrônica de Transmissão , Paclitaxel/administração & dosagem , Polietilenoglicóis/administração & dosagem , Compostos de Prata/administração & dosagem , Compostos de Prata/síntese química , Compostos de Prata/química , Análise Espectral RamanRESUMO
Seaweed lectins have been widely investigated as anti-nociceptive and anti-inflammatory agents. This study analyzed the anti-nociceptive and anti-inflammatory responses of a lectin from the green seaweed Caulerpa cupressoides (CcL) on zymosan-induced arthritis of the rat temporomandibular joint (TMJ). Rats received i.v. CcL 30 min prior to injection of zymosan (2mg/art.) or 0.9% saline into the left TMJ. Mechanical hyper-nociception was measured by the electronic von Frey method at baseline and 4h after zymosan injection. Animals were euthanized 6h after zymosan injection and the synovial fluid was collected for leukocyte counting and myeloperoxidase activity assessment. Other animals were treated with ZnPP-IX (3mg/kg; s.c.), a specific heme oxygenase-1 pathway inhibitor, and naloxone (10 µg/art.), a nonselective opioid receptor antagonist. TMJ tissues were excised to perform histopathological and immunohistochemistry analyses. CcL (0.1, 1 or 10mg/kg) significantly reduced zymosan-induced hyper-nociception (81, 83 and 89.5%, respectively) and inhibited the leukocyte influx (77.3, 80.7 and 98.5%, respectively) compared with the zymosan-only group, as confirmed by myeloperoxidase activity; however, treatment with naloxone or ZnPP-IX did not revert the effects of CcL (10mg/kg), suggesting that the naloxone-sensitive opioid and heme oxygenase-1 pathways are not involved. CcL also reduced the leukocyte influx and the expression of IL-1ß and TNF-α in the TMJ, based on histopathological and immunohistochemistry analyses, respectively. Therefore, CcL reduces TMJ hyper-nociception and inflammation with a mechanism that is partially dependent on TNF-α and IL-1ß inhibition. CcL reveals a potentially valuable alternative tool for future studies of TMJ disorders.
Assuntos
Artrite Experimental/tratamento farmacológico , Caulerpa/imunologia , Inflamação/tratamento farmacológico , Leucócitos/efeitos dos fármacos , Lectinas de Plantas/administração & dosagem , Membrana Sinovial/efeitos dos fármacos , Articulação Temporomandibular/efeitos dos fármacos , Administração Intravenosa , Animais , Artrite Experimental/induzido quimicamente , Movimento Celular/efeitos dos fármacos , Inflamação/induzido quimicamente , Interleucina-1beta/metabolismo , Leucócitos/imunologia , Masculino , Nociceptividade/efeitos dos fármacos , Peroxidase/metabolismo , Lectinas de Plantas/efeitos adversos , Ratos , Ratos Wistar , Membrana Sinovial/imunologia , Articulação Temporomandibular/patologia , Fator de Necrose Tumoral alfa/metabolismo , Zimosan/administração & dosagemRESUMO
We evaluated the polypeptide profiles, inhibition of human salivary α-amylase activity, and hemagglutination properties of a commercial phaseolamin sample. We also performed an in vivo assay to investigate the effects of a commercial phaseolamin treatment (100, 500, or 1500 mg/kg) over 20 days on the glycemia, body weight, and serum biochemical parameters (total cholesterol, triglycerides, alanine aminotransferase, and aspartate aminotransferase) of nondiabetic and streptozotocin-induced diabetic rats. The in vitro evaluation showed defined protein profiles, low hemagglutination activity, and high α-amylase inhibition. None of the experimental groups treated with phaseolamin or acarbose showed decreases in body weight. Our data demonstrate that phaseolamin inhibits amylase activity in vitro, reduces blood glucose levels, decreases or attenuates some of the renal and hepatic effects of diabetes in streptozotocin-induced rats, and could therefore have therapeutic potential in the treatment or prevention of the complications of diabetes.
Assuntos
Diabetes Mellitus Experimental/enzimologia , Inibidores Enzimáticos/administração & dosagem , Extratos Vegetais/administração & dosagem , Lectinas de Plantas/administração & dosagem , alfa-Amilases/antagonistas & inibidores , Animais , Glicemia/metabolismo , Peso Corporal/efeitos dos fármacos , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/metabolismo , Inibidores Enzimáticos/química , Humanos , Masculino , Phaseolus/química , Extratos Vegetais/química , Lectinas de Plantas/química , Ratos , Ratos Wistar , alfa-Amilases/metabolismoRESUMO
Acacia farnesiana lectin-like protein (AFAL) is a chitin-binding protein and has been classified as phytohaemagglutinin from Phaseolus vulgaris (PHA). Legume lectins are examples for structural studies, and this family of proteins shows a remarkable conservation in primary, secondary, and tertiary structures. Lectins have ability to reduce the effects of inflammation caused by phlogistic agents, such as carrageenan (CGN). This paper explains the anti-inflammatory activity of AFAL through structural comparison with anti-inflammatory legume lectins. The AFAL model was obtained by molecular modeling and molecular docking with glycan and carrageenan were performed to explain the AFAL structural behavior and biological activity. Pisum sativum lectin was the best template for molecular modeling. The AFAL structure model is folded as a ß sandwich. The model differs from template in loop regions, number of ß strands and carbohydrate-binding site. Carrageenan and glycan bind to different sites on AFAL. The ability of AFAL binding to carrageenan can be explained by absence of the sixth ß -strand (posterior ß sheets) and two ß strands in frontal region. AFAL can inhibit pathway inflammatory process by carrageenan injection by connecting to it and preventing its entry into the cell and triggers the reaction.
Assuntos
Anti-Inflamatórios/química , Inflamação/tratamento farmacológico , Modelos Moleculares , Lectinas de Plantas/química , Acacia , Animais , Anti-Inflamatórios/metabolismo , Carragenina/toxicidade , Quitina/química , Cristalografia por Raios X , Inflamação/induzido quimicamente , Inflamação/patologia , Camundongos , Simulação de Acoplamento Molecular , Fito-Hemaglutininas/química , Fito-Hemaglutininas/metabolismo , Lectinas de Plantas/administração & dosagem , Lectinas de Plantas/isolamento & purificação , Lectinas de Plantas/metabolismo , Ligação ProteicaRESUMO
The immunoregulatory effect of Artin M and jacalin from extract of Artocarpus integrifolia seeds (jack extract) against infection with Candida albicans was investigated. Swiss mice received jack extract containing 500 µg protein/ml PBS intraperitoneally (i.p.) or PBS alone and after 72 h were infected i.p. with C. albicans CR15 (10(7)) and sacrificed after 30 min, 2, 6, 24, and 72 h. ELISA analysis revealed that in jack extract-treated mice IFN-γ was predominantly produced versus IL-10 in control mice. These results suggest that jack extract induced a protective immune response, since C. albicans clearance was complete at 72 h postinfection. Jack extract presents two lectins (Artin M and jacalin) with distinct biological properties. Artin M was able to induce IL-12 production by macrophages. Also, Artin M in different concentrations, associated with jacalin or in jack extract induced both IFN-γ and IL-17 production. As a consequence, phagocytic and candidacidal activity increased significantly. Alanine aminotransferase activity (ALT) was used as parameter for damage of the liver. The activity of ALT correlated with inoculum size that increased significantly in control group, however, mice pretreated with jack extract 3 days before infection presented normal ALT. Mice pretreated with jack extract that received a lethal inoculum of Candida presented 90% survival versus 20% among controls or mice pretreated with jacalin. Thus, the results suggest that Artin M by itself, associated with jacalin or present in jack extract is able to induce protective Th1 and Th17 immune responses against Candida albicans infection.
Assuntos
Adjuvantes Imunológicos/administração & dosagem , Candida albicans/imunologia , Candidíase/imunologia , Fitoterapia , Extratos Vegetais/administração & dosagem , Lectinas de Plantas/administração & dosagem , Células Th1/efeitos dos fármacos , Células Th17/efeitos dos fármacos , Adjuvantes Imunológicos/efeitos adversos , Alanina Transaminase/genética , Alanina Transaminase/imunologia , Alanina Transaminase/metabolismo , Animais , Artocarpus/imunologia , Candida albicans/patogenicidade , Candidíase/prevenção & controle , Citocinas/genética , Citocinas/imunologia , Citocinas/metabolismo , Ativação Enzimática/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/imunologia , Imunidade/efeitos dos fármacos , Lectinas/administração & dosagem , Lectinas/efeitos adversos , Camundongos , Extratos Vegetais/efeitos adversos , Lectinas de Plantas/efeitos adversos , Sementes , Células Th1/imunologia , Células Th1/metabolismo , Células Th1/patologia , Células Th17/imunologia , Células Th17/metabolismo , Células Th17/patologiaRESUMO
This study investigated and compared vascular actions of leguminous lectins obtained from the Canavalia genus (Canavalia brasiliensis, Canavalia gladiata, and Canavalia maritima) in the rat models of paw edema and isolated aorta. Paw edema was induced by subcutaneous injection of lectins (0.01-1 mg/kg) in animals pre-treated or not with indomethacin or L-NAME. In isolated aorta, cumulative concentration curves of C. gladiata or C. brasiliensis (1-100 microg/ml) were performed at the contraction plateau induced by phenylephrine or at tissue basal tonus. The mechanism of the lectin relaxant action was investigated by previous addition of L-NAME, indomethacin, or tetraethylammonium. In both models, the lectin domain involvement was evaluated by incubation of lectins with their ligand and non-ligand sugars. The lectins induced paw edema paralleled by protein leakage. The edematogenic activity elicited by C. gladiata and C. brasiliensis involves prostaglandins and nitric oxide (NO), while that of C. maritima occurs without NO interference. C. gladiata and C. brasiliensis elicited aorta relaxation involving NO and prostacyclin, while that of C. gladiata included EDHF. All lectin effects were prevented by their binding sugars. The present study demonstrated important vasodilator effects of different degrees and mechanisms in vivo and in vitro of Canavalia lectins. In vivo, the edematogenic activity was paralleled by plasma exudation, and in vitro, aorta relaxation was strictly dependent on intact endothelium. All effects occurred via interaction with lectin domains and participation of NO and/or prostanoids.
Assuntos
Canavalia/química , Lectinas de Plantas/farmacologia , Vasodilatação/efeitos dos fármacos , Vasodilatadores/farmacologia , Animais , Aorta Torácica/efeitos dos fármacos , Aorta Torácica/metabolismo , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Edema/induzido quimicamente , Edema/fisiopatologia , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/metabolismo , Masculino , Óxido Nítrico/metabolismo , Lectinas de Plantas/administração & dosagem , Lectinas de Plantas/isolamento & purificação , Prostaglandinas/metabolismo , Ratos , Ratos Wistar , Vasodilatadores/administração & dosagem , Vasodilatadores/isolamento & purificaçãoRESUMO
The aim of the present study was to evaluate the potential antinociceptive and toxicity of Canavalia boliviana lectin (CboL) using different methods in mice. The role of carbohydrate-binding sites was also investigated. CboL given to mice daily for 14 days at doses of 5 mg/kg did not cause any observable toxicity. CboL (1, 5, and 10 mg/kg) administered to mice intravenously inhibited abdominal constrictions induced by acetic acid and the two phases of the formalin test. In the hot plate and tail immersion tests, the same treatment of CboL induced significant increase in the latency period. In the hot plate test, the effect of CboL (5 mg/kg) was reversed by naloxone (1 mg/kg), indicating the involvement of the opioid system. In the open-field and rota-rod tests, the CboL treatment did not alter animals' motor function. These results show that CboL presents antinociceptive effects of both central and peripheral origin, involving the participation of the opioid system via lectin domain.
Assuntos
Analgésicos/farmacologia , Canavalia/química , Dor/tratamento farmacológico , Lectinas de Plantas/farmacologia , Analgésicos/administração & dosagem , Analgésicos/toxicidade , Animais , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Masculino , Camundongos , Medição da Dor , Lectinas de Plantas/administração & dosagem , Lectinas de Plantas/toxicidade , SementesRESUMO
The lectin from the legume Vatairea macrocarpa is a galactose/N-acetylgalactosamine binding protein that induced cellular inflammatory response mediated by resident cells. This study investigated which inflammatory mediators would be released from lectin-activated cells. The intraperitoneal injection in rats of the supernatant from cultured macrophages, but not from mast cells, stimulated with lectin induced a time- and dose-dependent release of a neutrophil chemotactic factor, termed MNCF-VML. Pharmacological modulation with dexamethasone inhibited both the lectin-induced chemotactic activity in vivo and also the lectin-induced release of MNCF-VML into the supernatant of cultured macrophages. Cyclooxygenase and lipoxygenase metabolites are apparently not involved in the action of this factor or its release, since indomethacin or MK886 were unable to affect the lectin response. The molecular weight of MNCF-VML was found to be greater than 5 kDa, which led to the investigation of which cytokine(s) could be involved by the following approaches: (a) treatment of animals with antiserum to tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-1, or IL-8 before intraperitoneal injection of lectin and (b) addition of antiserum to TNF-alpha, IL-1, or IL-8 to the supernatant of lectin-stimulated macrophages before intraperitoneal administration. Antiserum to TNF-alpha, but not IL-1 nor IL-8, inhibited the neutrophil migration induced either by lectin or MNCF-VML. Our data suggest that neutrophil migration induced by V. macrocarpa lectin occurs via the release of cytokines such as TNF-alpha by macrophages. Thus, this lectin may represent an important tool to better understand pathological situations where an excess of leukocytes at inflammatory sites causes tissue injury.
Assuntos
Fatores Quimiotáticos/metabolismo , Fabaceae/química , Macrófagos/efeitos dos fármacos , Lectinas de Plantas/farmacologia , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/farmacologia , Células Cultivadas , Fatores Quimiotáticos/química , Quimiotaxia de Leucócito/efeitos dos fármacos , Dexametasona/administração & dosagem , Dexametasona/farmacologia , Relação Dose-Resposta a Droga , Feminino , Indóis/administração & dosagem , Indóis/farmacologia , Indometacina/administração & dosagem , Indometacina/farmacologia , Injeções Intraperitoneais , Interleucina-8/metabolismo , Inibidores de Lipoxigenase/administração & dosagem , Inibidores de Lipoxigenase/farmacologia , Macrófagos/citologia , Macrófagos/metabolismo , Mastócitos/citologia , Mastócitos/efeitos dos fármacos , Mastócitos/metabolismo , Peso Molecular , Neutrófilos/citologia , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Lectinas de Plantas/administração & dosagem , Lectinas de Plantas/química , Ratos , Ratos Wistar , Sementes/química , Fator de Necrose Tumoral alfa/metabolismoRESUMO
This paper describes the purification and characterization of a new N-acetyl-d-glucosamine-specific lectin from Araucaria angustifolia (AaL) seeds (Araucariaceae) and its anti-inflammatory and antibacterial activities. AaL was purified using a combination of affinity chromatography on a chitin column and ion exchange chromatography on Sephacel-DEAE. The pure protein has 8.0kDa (SDS-PAGE) and specifically agglutinates rabbit erythrocytes, effect that was independent of the presence of divalent cations and was inhibited after incubation with glucose and N-acetyl-d-glucosamine. AaL showed antibacterial activity against Gram-negative and Gram-positive strains, shown by scanning electron microscopy. AaL, intravenously injected into rats, showed anti-inflammatory effect, via carbohydrate site interaction, in the models of paw edema and peritonitis. This lectin can be used as a tool for studying bacterial infections and inflammatory processes.
Assuntos
Bactérias/citologia , Bactérias/efeitos dos fármacos , Cycadopsida/metabolismo , Inflamação/tratamento farmacológico , Lectinas de Plantas/administração & dosagem , Sementes/química , Animais , Relação Dose-Resposta a Droga , Extratos Vegetais/administração & dosagem , Extratos Vegetais/isolamento & purificação , Lectinas de Plantas/isolamento & purificação , RatosRESUMO
In the present study we evaluated Canavalia brasiliensis (ConBr), Pisum arvense (PAA) and Artocarpus integrifolia (KM+) lectins as immunostimulatory molecules in vaccination against Leishmania amazonensis infection. Although they induced IFN-gamma production, the combination of the lectins with SLA antigen did not lead to lesion reduction. However, parasite load was largely reduced in mice immunized with KM+ lectin and SLA. KM+ induced a smaller inflammatory reaction in the air pouch model and was able to inhibit differentiation of dendritic cells (BMDC), but to induce maturation by enhancing the expression of MHC II, CD80 and CD86. These observations indicate the modulatory role of plant lectins in leishmaniasis vaccination may be related to their action on the initial innate response.
Assuntos
Adjuvantes Imunológicos/farmacologia , Antígenos de Protozoários/imunologia , Leishmania/imunologia , Leishmaniose/imunologia , Lectinas de Plantas/farmacologia , Adjuvantes Imunológicos/administração & dosagem , Estruturas Animais/parasitologia , Animais , Antígenos de Protozoários/administração & dosagem , Artocarpus , Canavalia , Células Dendríticas/química , Células Dendríticas/imunologia , Feminino , Antígenos de Histocompatibilidade Classe II/análise , Interferon gama/biossíntese , Interleucina-12/biossíntese , Leishmaniose/patologia , Linfócitos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Lectinas de Plantas/administração & dosagemRESUMO
This study was aimed at determining the effect of raw full-fat soybean in the digestion/absorption of broilers and to evaluate the role of soybean agglutinin (SBA) in the pathogenesis of the runting and stunting syndrome of broilers. Six broiler groups were fed with six experimental diets for 42 days in which the defatted soybean meal of the basal diet was substituted with increasing raw full-fat soybean percentages ranging from 0 to 100%. The results suggested that SBA included in the raw full-fat soybean can play a role in the pathogenesis of the runting and stunting syndrome in broilers since binding and incorporation of SBA to enterocytes were associated with hyperplasia and dysplasia of the intestinal epithelium, as suggested by the progressive increases (P < 0.001) of mitosis/crypt in the duodenum and in the midintestine, as well as by intestinal villi atrophy (P < 0.001) in the duodenum and the midintestine. Indigestion and malabsorption of proteins induced progressive low weight gain (P < 0.001) up to 50% with 100% of raw full-fat soybean in the diet at 42 days. Xanthophylls in serum and skin yellowness were enhanced linearly in the broilers fed 42 days with increasing percentages of raw full-fat soybean in the diet. Increased xanthophylls absorption and decreased protein absorption can be caused by pathological changes in the epithelial intestine triggered by SBA binding and internalization.
Assuntos
Galinhas , Gorduras na Dieta/administração & dosagem , Glycine max/química , Carne , Pigmentação , Lectinas de Plantas/administração & dosagem , Proteínas de Soja/administração & dosagem , Animais , Dieta , Transtornos do Crescimento/etiologia , Transtornos do Crescimento/patologia , Transtornos do Crescimento/veterinária , Absorção Intestinal , Intestinos/patologia , Lectinas de Plantas/efeitos adversos , Doenças das Aves Domésticas/etiologia , Doenças das Aves Domésticas/patologia , Proteínas de Soja/efeitos adversos , Glycine max/efeitos adversosRESUMO
This present work was undertaken to answer two basic questions (a) is C. argentea lectin part of the general defensive strategy of the plant against predation by animals? (b) if so, how does it act on them? To achieve these goals the lectin from C. argentea seeds was purified to homogeneity and included at a 2% level in a diet containing 10% total protein and given to growing rats for 10 days. In vivo it was noted that the lectin from C. argentea is resistant to gut proteolysis, binds to the cells lining the small intestine and induces enlargement in the small intestine, caecum and colon, kidneys and pancreas compared to control rats exposed to the egg-white diet (EW). As the diet containing the purified C. argentea lectin has the same basic composition and protein content of EW diet, the small intestine, kidney and pancreas enlargements are clearly lectin-specific effects. Moreover the animals exposed to the lectin-containing diet presented a significant reduction in the growth rate and lower values of digestibility, NPU and biological value compared to animals fed on a control lectin-free diet. Thus the data from this present study and the report that the C. argentea lectin has insecticidal activity upon Callosobruchus maculatus larvae which attacks cowpea (Vigna unguiculata) seeds reinforce the hypothesis that lectins take part in the mechanisms against herbivory.
Assuntos
Sistema Digestório/efeitos dos fármacos , Fabaceae/química , Insetos/efeitos dos fármacos , Lectinas de Plantas/farmacologia , Ratos Wistar/crescimento & desenvolvimento , Animais , Bioensaio , Digestão/efeitos dos fármacos , Sistema Digestório/patologia , Eletroforese em Gel de Poliacrilamida , Fezes/química , Hemaglutinação/efeitos dos fármacos , Imuno-Histoquímica , Insetos/crescimento & desenvolvimento , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Masculino , Tamanho do Órgão/efeitos dos fármacos , Lectinas de Plantas/administração & dosagem , Lectinas de Plantas/química , Lectinas de Plantas/isolamento & purificação , Distribuição Aleatória , Ratos , Sementes/química , Aumento de Peso/efeitos dos fármacosRESUMO
The effects of the snowdrop lectin, Galanthus nivalis agglutinin (GNA), delivered through an artificial diet, on growth, development, and life history parameters of the Mexican rice borer, Eoreuma loftini (Dyar), were evaluated in the laboratory. Incorporation of GNA at three treatment levels, 0.5, 1.0, and 2.0% of total dietary protein, in the larval diet significantly decreased larval survivorship and percentage of adults emerging relative to a control diet lacking GNA, whereas differences were not observed among the three treatment levels. Both larvae and pupae in the control were 8-25% larger than those in the GNA treatments, but differences were not observed between larvae in the GNA treatments. Furthermore, presence of GNA did not affect larval and pupal developmental periods, longevities, and fecundities compared with the control. Mexican rice borer life history parameters, such as net reproductive rate and intrinsic rate of increase, were substantially reduced by the presence of GNA in the diet, but differences were not evident among the three GNA treatment levels.