RESUMO
Tumor cells may develop alterations in glycosylation patterns during the initial phase of carcinogenesis. These alterations may be important therapeutic targets for lectins with antitumor action. This work aimed to evaluate the in vitro cytotoxicity of VML on tumor and non-tumor cells (concentration of 25 µg/mL and then microdiluted) and evaluate its in vivo toxicity at different concentrations (1.8, 3.5 and 7.0 µg/mL), using Drosophila melanogaster. Toxicity in D. melanogaster evaluated mortality rate, as well as oxidative stress markers (TBARS, iron levels, nitric oxide levels, protein and non-protein thiols). The cytotoxicity assay showed that VML had cytotoxic effect on leukemic lines HL-60 (IC50 = 3.5 µg/mL), KG1 (IC50 = 18.6 µg/mL) and K562 (102.0 µg/mL). In the toxicity assay, VML showed no reduction in survival at concentrations of 3.5 and 7.0 µg/mL and did not alter oxidative stress markers at any concentrations tested. Cytotoxicity of VML from HL-60, KG1 and K562 cells could arise from the interaction between the lectin and specific carbohydrates of tumor cells. In contrast, effective concentrations of VML against no-tumor cells human keratinocyte - HaCat and in the D. melanogaster model did not show toxicity, suggesting that VML is a promising molecule in vivo studies involving leukemic cells.
Assuntos
Drosophila melanogaster , Lectinas , Animais , Humanos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Drosophila melanogaster/efeitos dos fármacos , Células HL-60 , Lectinas/farmacologia , Lectinas/toxicidade , Estresse Oxidativo/efeitos dos fármacosRESUMO
Punica granatum, popularly known as pomegranate, is a fruit tree with wide worldwide distribution, containing numerous phytochemicals of great medicinal value. The aim of the present study was to determine the phytochemical profile and antioxidant potential of a protein fraction (PF) derived from P. granatum sarcotesta which is rich in lectin. In addition, the acute oral toxicity, genotoxicity and antigenotoxicity of this protein fraction (PF) from P. granatum sarcotesta was measured. The phytochemical profile of PF was determined using HPLC. The in vitro antioxidant effect was assessed using the methods of total antioxidant capacity (TAC) and DPPH and ABTS+ radical scavenging. Acute oral toxicity was determined in female Swiss mice administered a single dose of 2000 mg/kg. This PF was examined for genotoxicity and antigenotoxicity at doses of 500, 1000 and 2000 mg/kg, utilizing mouse peripheral blood cells. Phytochemical characterization detected a high content of ellagic acid and antioxidant capacity similar to that of ascorbic acid (positive control). PF was not toxic (LD50 >2000 mg/kg) and did not exert a genotoxic effect in mice. PF protected the DNA of peripheral blood cells against damage induced by cyclophosphamide. In conclusion, this PF fraction exhibited significant antioxidant activity without initiating toxic or genotoxic responses in mice.
Assuntos
Antioxidantes , Extratos Vegetais , Punica granatum , Animais , Camundongos , Antioxidantes/farmacologia , Feminino , Extratos Vegetais/toxicidade , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Punica granatum/química , Lectinas/toxicidade , Testes de Mutagenicidade , Dano ao DNA/efeitos dos fármacos , Testes de Toxicidade AgudaRESUMO
The evaluation of ecotoxicity of mosquito larvicidal agents (such as the water-soluble lectin from Moringa oleifera seeds, WSMoL) is an essential step to establish the guidelines for their use. In this sense, this work evaluated the toxicity of WSMoL to Danio rerio embryos and larvae. Embryos were exposed to waterborne WSMoL (0.0125-0.2 mg mL-1) for 96 h and lethal and sub-lethal effects were observed every 24 h. In the bioassays with larvae, the individuals were exposed to the WSMoL (0.025-0.2 mg mL-1), mortality was recorded daily, and larval swimming velocities were analyzed after 72 h and 168 h of exposure. Additionally, acetylcholinesterase (AChE) activity of larvae was determined after 168 h of exposure. WSMoL LC50 values to embryos were 0.190, 0.133 and 0.049 mg mL-1 after 48, 72 and 96 h, respectively. No toxic endpoint was observed after exposure for 24 h. In addition, hatching was delayed and larval length at 96 h was reduced compared to the control. WSMoL LC50 to larvae were 0.21 and 0.135 mg mL-1, after 24 h and 96 h, respectively. Larvae exposed to 0.1 and 0.2 mg mL-1 showed a decrease in swimming speed and a significant reduction in AChE activity. In conclusion, WSMoL at waterborne concentrations needed for its use as a larvicide to A. aegypti causes lethal and sublethal effects to zebrafish embryos and larvae. Therefore, its use in waterbodies where there are non-target organisms is not recommended.
Assuntos
Embrião não Mamífero/efeitos dos fármacos , Lectinas/toxicidade , Moringa oleifera/toxicidade , Peixe-Zebra/embriologia , Animais , Larva , Sementes , Água , Poluentes Químicos da Água/toxicidadeRESUMO
Lectins are a large group of proteins found in animals, plants, fungi, and bacteria that recognize specific carbohydrate targets and play an important role in cell recognition and communication, host-pathogen interactions, embryogenesis, and tissue development. Recently, lectins have emerged as important biomedical tools that have been used in the development of immunomodulatory, antipathogenic, and anticancer agents. Several lectins have been shown to have the ability to discriminate between normal cells and tumor cells as a result of their different glycosylation patterns. Furthermore, the specific binding of lectins to cancer cells has been shown to trigger mechanisms that can promote the death of these abnormal cells. Here, we review the importance of lectins-carbohydrates interactions in cancer therapy and diagnosis. We examine the use of lectins in the modification of nanoparticles (liposomes, solid lipid nanoparticles and other polymers) for anticancer drug delivery. The development of drug delivery systems (liposomes, alginate/chitosan microcapsules, alginate beads) carrying some antitumor lectins is also discussed. In these cases, the processes of cell death induced by these antitumor lectins were also showed (if available). In both cases (lectin-conjugated polymers or encapsulated lectins), these new pharmaceutical preparations showed improved intracellular delivery, bioavailability and targetability leading to enhanced therapeutic index and significantly less side effects.
Assuntos
Amino Açúcares/metabolismo , Antineoplásicos/química , Lectinas/metabolismo , Amino Açúcares/química , Antineoplásicos/uso terapêutico , Antineoplásicos/toxicidade , Apoptose/efeitos dos fármacos , Técnicas Biossensoriais , Portadores de Fármacos/química , Glicosilação , Humanos , Lectinas/química , Lectinas/uso terapêutico , Lectinas/toxicidade , Nanopartículas/química , Neoplasias/diagnóstico , Neoplasias/tratamento farmacológicoRESUMO
As leishmanioses constituem um grupo de doenças crônicas causadas por protozoários pertencentes ao gênero Leishmania. Tendo em vista a complexidade e ineficácia dos tratamentos atuais, o desenvolvimento de novas drogas menos tóxicas ainda é uma necessidade. Na prospecção de possíveis agentes quimioterápicos contra as leishmanioses, as lectinas apresentam-se como candidatos promissores por apresentarem um amplo espectro de atividades biológicas. No presente trabalho nós investigamos o potencial leishmanicida e imunomodulador da lectina Onil. Nossos resultados demonstraram que a Onil apresentou baixa toxidade sobre células do exsudato peritoneal (CEP) de camundongos (CC50= 317,5 ± 0,6 µg/mL), foi efetiva ao inibir o crescimento de formas promastigotas de L. braziliensis (IC50=150,58± 0,78 µg/mL), mostrou-se mais seletiva para o parasito do que para célula do hospedeiro (ISe=2,1). No entanto, não foi capaz de inibir a sobrevivência das amastigotas no interior das CEPs. A lectina Onil causou alterações ultraestruturais no flagelo, bem como mostrou um efeito sobre a divisão celular de formas promastigotas. A marcação das células tratadas com Anexina V (AV) e Iodeto de Propídio (IP) mostrou uma pequena subpopulação de células apresentava marcação para AV/IP compatíveis com o processo de morte celular por necrose/apoptose tardia. A marcação das células controles e tratadas com Onil com Rho 123 revelou que na grande maioria das células o potencial de membrana mitocondrial foi preservado. O tratamento com a lectina (75-300 µg/mL) não alterou significativamente a produção de NO e não induziu alterações na produção de citocinas em CEPs infectadas L. braziliensis. Por outro lado, uma intensa produção de citocinas associadas aos perfis Th1, Th2 e Th17 foi observada em CEPs não infectadas tratadas com 30 µg/mL da Onil. Nossos dados apontam para uma possível utilização da Onil como agente adjuvante ou como carreadora de drogas para o tratamento da leishmaniose cutânea
Leishmaniasis comprises a group of disease caused by protozoa belonging to the Leishmaniagenus. Taking in account the complexity and inefficiency of current treatments against leishmaniasis, the development of new, less toxic drugs is still needed. In a search of potential chemotherapeutic agents against leishmaniasis, lectins presented as promising candidates because for presenting a broad spectrum of biological activities. In this regard, in the present study we investigated the leishmanicidal and immunomodulatory activities of Onil in vitro. Our results demonstrated that Onil presented low toxicity to mice peritoneal exudate cells (PEC) (CC50= 317.5 ± 0.6 μg / mL), was effective to inhibit the growth of promastigotes of Leishmania braziliensis (IC50= 150.58 ± 0.78 μg / mL) and was shown to be more selective for the parasite than to the host cell, with SeI=2.1...
Assuntos
Camundongos , Antiprotozoários/farmacologia , Leishmaniose Cutânea , Lectinas/toxicidade , Lectinas/uso terapêutico , Leishmania braziliensis , Leishmania braziliensis/ultraestrutura , Antiprotozoários/toxicidade , Antiprotozoários/uso terapêutico , Ciclídeos/sangue , Exsudatos e TransudatosRESUMO
In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3-1.35%, w/v) for 8 days, in presence or absence of food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglycone flavonoids, cinnamic acid derivatives, traces of steroids, and lectin activity were detected in the extract, which killed the larvae at an LC50 of 0.62% (unfed larvae) and 1.03% (fed larvae). Further, the larvae incubated with the extract reacted by eliminating the gut content. No larvae reached the pupal stage in treatments at concentrations between 0.5% and 1.35%, while in the control (fed larvae), 61.7% of individuals emerged as adults. The extract (1.0%) promoted intense disorganization of larval midgut epithelium, including deformation and hypertrophy of cells, disruption of microvilli, and vacuolization of cytoplasms, affecting digestive, enteroendocrine, regenerative, and proliferating cells. In addition, cells with fragmented DNA were observed. Separation of extract components by solid phase extraction revealed that cinnamic acid derivatives and flavonoids are involved in larvicidal effect of the extract, being the first most efficient in a short time after larvae treatment. The lectin present in the extract was isolated, but did not show deleterious effects on larvae. The extract and cinnamic acid derivatives were toxic to A. salina nauplii, while the flavonoids showed low toxicity. S. terebinthifolius leaf extract caused damage to the midgut of A. aegypti larvae, interfering with survival and development. The larvicidal effect of the extract can be attributed to cinnamic acid derivatives and flavonoids. The data obtained using A. salina indicates that caution should be used when employing this extract as a larvicidal agent.
Assuntos
Aedes/efeitos dos fármacos , Anacardiaceae/química , Intestinos/efeitos dos fármacos , Extratos Vegetais/toxicidade , Aedes/crescimento & desenvolvimento , Anacardiaceae/metabolismo , Animais , Cinamatos/química , Cinamatos/isolamento & purificação , Cinamatos/toxicidade , Fragmentação do DNA/efeitos dos fármacos , Flavonoides/química , Flavonoides/isolamento & purificação , Flavonoides/toxicidade , Mucosa Intestinal/metabolismo , Intestinos/patologia , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Lectinas/química , Lectinas/isolamento & purificação , Lectinas/toxicidade , Microscopia Eletrônica de Transmissão , Microscopia de Fluorescência , Extratos Vegetais/química , Folhas de Planta/química , Folhas de Planta/metabolismo , Extração em Fase SólidaRESUMO
BACKGROUND: The spread of the invasive snail Pomacea canaliculata is expanding the rat lungworm disease beyond its native range. Their toxic eggs have virtually no predators and unusual defenses including a neurotoxic lectin and a proteinase inhibitor, presumably advertised by a warning coloration. We explored the effect of egg perivitellin fluid (PVF) ingestion on the rat small intestine morphology and physiology. METHODOLOGY/PRINCIPAL FINDINGS: Through a combination of biochemical, histochemical, histopathological, scanning electron microscopy, cell culture and feeding experiments, we analyzed intestinal morphology, growth rate, hemaglutinating activity, cytotoxicity and cell proliferation after oral administration of PVF to rats. PVF adversely affects small intestine metabolism and morphology and consequently the standard growth rate, presumably by lectin-like proteins, as suggested by PVF hemaglutinating activity and its cytotoxic effect on Caco-2 cell culture. Short-term effects of ingested PVF were studied in growing rats. PVF-supplemented diet induced the appearance of shorter and wider villi as well as fused villi. This was associated with changes in glycoconjugate expression, increased cell proliferation at crypt base, and hypertrophic mucosal growth. This resulted in a decreased absorptive surface after 3 days of treatment and a diminished rat growth rate that reverted to normal after the fourth day of treatment. Longer exposure to PVF induced a time-dependent lengthening of the small intestine while switching to a control diet restored intestine length and morphology after 4 days. CONCLUSIONS/SIGNIFICANCE: Ingestion of PVF rapidly limits the ability of potential predators to absorb nutrients by inducing large, reversible changes in intestinal morphology and growth rate. The occurrence of toxins that affect intestinal morphology and absorption is a strategy against predation not recognized among animals before. Remarkably, this defense is rather similar to the toxic effect of plant antipredator strategies. This defense mechanism may explain the near absence of predators of apple snail eggs.
Assuntos
Intestino Delgado/patologia , Óvulo/química , Caramujos , Animais , Células CACO-2 , Dieta , Hemaglutinação , Humanos , Hipertrofia , Mucosa Intestinal/crescimento & desenvolvimento , Mucosa Intestinal/patologia , Intestino Delgado/crescimento & desenvolvimento , Espécies Introduzidas , Lectinas/toxicidade , Masculino , Óvulo/parasitologia , Ratos , Ratos WistarRESUMO
A novel lectin, HGA-2, was isolated from the sea cucumber Holothuria grisea. The protein was isolated by a single chromatographic step using a column of Guar Gum as affinity. HGA-2 showed an apparent molecular mass of 17 kDa and 34 kDa under reducing and nonreducing conditions, respectively. The hemagglutinating activity was specific for rabbit erythrocytes, showing no activity for human blood A, B and O. Its hemagglutinating activity was inhibited by carbohydrates containing galactose, with higher affinity for GalNAc and glycoprotein porcine stomach mucin (PSM). HGA-2 was stable at pH 6-10, significantly declining at pH 5 and a temperature of 40°C, with its activity being abolished at 100 °C. The HGA-2 protein was found to be Ca(2+)-dependent; it was highly toxic against Artemia nauplii and able to recognize and agglutinate cells of Escherichia coli. Amino acid sequences of tryptic peptides of HGA-2 strongly suggest that HGA-2 is a member of the C-type lectin family.
Assuntos
Aglutininas/química , Aglutininas/metabolismo , Escherichia coli/metabolismo , Galactosídeos/metabolismo , Holothuria/química , Lectinas/química , Lectinas/metabolismo , Aglutininas/isolamento & purificação , Aglutininas/toxicidade , Sequência de Aminoácidos , Animais , Hemaglutinação , Testes de Hemaglutinação , Humanos , Concentração de Íons de Hidrogênio , Íons , Lectinas/isolamento & purificação , Lectinas/toxicidade , Lectinas Tipo C , Dados de Sequência Molecular , Coelhos , Alinhamento de Sequência , TemperaturaRESUMO
The aim of this study was to determine the binding patterns of Canavalia ensiformis (ConA), Canavalia boliviana (ConBol) and Canavalia brasiliensis (ConBr) lectins to bovine sperm and their effects on sperm motility, viability, lipid peroxidation, reactive oxygen species production and fertilization ability. ConA bound to whole spermatozoa, with the exception of the equatorial segment, ConBol did not interact with the acrosome region and ConBr exhibited a fragmented binding pattern. The three lectins decreased sperm motility but did not affect cell viability or lipid peroxidation. Nevertheless, ROS production was increased in comparison to controls and a reduction in the cleavage and blastocyst ratio was induced in comparison to controls. In conclusion, this study determined that structurally similar lectins interact differently with bovine sperm and affect sperm motility, viability, lipid peroxidation, ROS production and fertilization ability in various ways.
Assuntos
Canavalia , Lectinas/toxicidade , Espermatozoides/efeitos dos fármacos , Animais , Bovinos , Feminino , Fertilização/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Oócitos/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Sementes , Motilidade dos Espermatozoides/efeitos dos fármacos , Espermatozoides/fisiologiaRESUMO
Lectins are sugar-binding proteins widely distributed in nature with many biological functions. Although many lectins have a remarkable biotechnological potential, some of them can be cytotoxic. Thus, the aim of this study was to assess the toxicity of five lectins, purified from seeds of different species of Canavalia genus. In order to determine the toxicity, assays with Artemia nauplii were performed. In addition, a fluorescence assay was carried out to evaluate the binding of lectins to Artemia nauplii. In order to verify the relationship between the structure of lectins and their cytotoxic effect, structural analysis was carried out to evaluate the volume of the carbohydrate recognition domain (CRD) of each lectin. The results showed that all lectins exhibited different toxicities and bound to a similar area in the digestive tract of Artemia nauplii. Concerning the structural analysis, differences in spatial arrangement and volume of CRD may explain the variation of the toxicity exhibited by each lectin. To this date, this is the first study that establishes a link between toxicity and structure of CRD from Diocleinae lectins.
Assuntos
Artemia/efeitos dos fármacos , Artemia/metabolismo , Lectinas/toxicidade , Sequência de Aminoácidos , Animais , Artemia/química , Canavalia/toxicidade , Carboidratos/química , Lectinas/química , Lectinas/metabolismo , Sementes/químicaRESUMO
UNLABELLED: This article reports the genotoxicity assessment of an extract of M. oleifera seed powder and the water-soluble Moringa oleifera lectin (WSMoL) isolated from seeds. The lectin isolated by chitin chromatography showed hemagglutinating activity with different erythrocytes, activity in a broad pH range (4.5 to 9.5), and retention of hemagglutinating activity after being heated to 100 °C. Genotoxicity of the seed extract and WSMoL were assessed using the cell-free plasmid DNA as well as the Salmonella typhimurium (Ames and Kado) assays with TA97, TA98, TA100, and TA102 in the presence or absence of hepatic metabolization. Seed extract at concentration (0.2 µg/µL) recommended to treat water was not genotoxic by Ames, Kado, and cell-free plasmid DNA assays. S. typhimurium strains showed to be sensitive to M. oleifera extract revealing a mutagenic effect at doses higher than 0.6 µg/µL with hepatic metabolization. The extract at doses higher than 0.4 µg/µL, without hepatic metabolization, was mutagenic for TA100 and TA102. WSMoL was nonmutagenic by used assays. The use of high concentrations of the extract may pose a risk to human health and the safe use of M. oleifera seed powder to treat water for human consumption requires more study; however, the purified lectin could be an alternative for water treatment. PRACTICAL APPLICATION: The concentration 0.2 µg/µL of M. oleifera seed extract recommended to treat water for humans did not pose a risk to human health. The mutagenicity detected at concentrations higher than 0.4 µg/µL was not due to WSMoL, lectin isolated from extract.
Assuntos
Dano ao DNA , Lectinas/toxicidade , Moringa oleifera/química , Extratos Vegetais/toxicidade , Sementes/química , Inocuidade dos Alimentos , Hemaglutinação , Concentração de Íons de Hidrogênio , Lectinas/análise , Mutagênicos/toxicidade , Extratos Vegetais/análise , Fatores de Risco , Salmonella typhimurium/efeitos dos fármacosRESUMO
Snake venom lectins have been studied in regard to their chemical structure and biological functions. However, little is known about lectins isolated from Bothrops atrox snake venom. We report here the isolation and partial functional and biochemical characterization of an acidic glycan-binding protein called galatrox from this venom. This lectin was purified by affinity chromatography using a lactosyl-sepharose column, and its homogeneity and molecular mass were evaluated by high-performance liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. The purified galatrox was homogeneous and characterized as an acidic protein (pI 5.2) with a monomeric and dimeric molecular mass of 16.2 and 32.5 kDa, respectively. Alignment of N-terminal and internal amino acid sequences of galatrox indicated that this protein exhibits high homology to other C-type snake venom lectins. Galatrox showed optimal hemagglutinating activity at a concentration of 100 µg/ml and this effect was drastically inhibited by lactose, ethylenediaminetetraacetic acid, and heating, which confirmed galatrox's lectin activity. While galatrox failed to induce the same level of paw edema or mast cell degranulation as B. atrox crude venom, galatrox did alter cellular viability, which suggested that galatrox might contribute to venom toxicity by directly inducing cell death.
Assuntos
Bothrops/metabolismo , Lectinas/genética , Lectinas/isolamento & purificação , Venenos de Serpentes/química , Venenos de Serpentes/isolamento & purificação , Venenos de Víboras/química , Venenos de Víboras/isolamento & purificação , Sequência de Aminoácidos , Animais , Morte Celular/efeitos dos fármacos , Linhagem Celular/citologia , Linhagem Celular/efeitos dos fármacos , Cromatografia de Afinidade/métodos , Cromatografia Líquida de Alta Pressão/métodos , Edema/induzido quimicamente , Lectinas/química , Lectinas/metabolismo , Lectinas/toxicidade , Masculino , Mastócitos/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Ratos , Homologia de Sequência de Aminoácidos , Venenos de Serpentes/genética , Venenos de Serpentes/metabolismo , Venenos de Serpentes/toxicidade , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Venenos de Víboras/toxicidadeRESUMO
Schistosomiasis or bilharzia, which affects millions of people living in Africa, Asia and Latin America, is closely associated with certain species of aquatic snails. One way of attacking the disease is to eradicate the host snails. Molluscicidal activities of natural compounds are especially important in the widespread control of this tropical disease. As part of our search for natural compounds with molluscicidal properties for the vector control of schistosomiasis, we are now evaluating for the first time the toxicity of the plant lectins from Canavalia brasiliensis (ConBr), Cratylia floribunda (CFL), Dioclea guianensis (Dgui), Dioclea grandiflora (DGL) and Dioclea virgata (Dvir) to Biomphalaria glabrata Say and Artemia salina Leach. Results indicate that all the samples were toxic to A. salina Leach, some of them with values of lethal concentration that kills 90% of the population (LC(90))<10 microg mL(-1). They are also active against B. glabrata Say, killing 100% of adult snails, at a concentration of 50 microg mL(-1). The lectins CFL and Dgui possess properties lethal to mollusks, with values of LC(90)=50.3 microg mL(-1) and LC(90)=41.0 microg mL(-1), respectively.
Assuntos
Artemia/metabolismo , Biomphalaria/metabolismo , Glucose/metabolismo , Lectinas/toxicidade , Manose/metabolismo , Animais , Lectinas/isolamento & purificação , Lectinas/metabolismoRESUMO
Aedes aegypti larvae have developed tolerance to many insecticides used for mosquito control. Moringa oleifera seeds contain a water-soluble lectin (WSMoL) and this paper reports the effect of M. oleifera seed extracts (MoE(1-15)) and WSMoL on development and survival of A. aegypti larvae. WSMoL peptide from in-gel trypsin digestion is also described. MoE(1-15) showed hemagglutinating activity and WSMoL had similarity with flocculating proteins from M. oleifera seeds. MoE(1) and MoE(3) delayed larval development which stopped in the third instar (L3) in MoE(6) and MoE(15). Significant (p<0.0001) larval mortality was only detected in MoE(15). Native WSMoL showed larvicidal activity (LC(50) 0.197 mg mL(-1)) and heated lectin, without hemagglutinating activity, did not kill fourth instar (L4) larvae. Optical microscopy showed that live L4 from MoE(1) presented underlying epithelium, increased gut lumen and hypertrophic segments; dead L4 from WSMoL were absent of underlying epithelium, had increased gut lumen and hypertrophic segments. The presence of hemagglutinating activity in the extracts suggests that soluble lectin promotes the delay of larval development and mortality; furthermore, the absence of larvicidal activity in heat-denatured WSMoL strengthens the involvement of lectin in this activity mechanism.
Assuntos
Aedes/efeitos dos fármacos , Inseticidas/toxicidade , Lectinas/toxicidade , Moringa oleifera/química , Aedes/crescimento & desenvolvimento , Animais , Monitoramento Ambiental , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Controle de Mosquitos , Sementes/química , Testes de ToxicidadeRESUMO
As plantas medicinais têm sido usadas desde a antiguidade no tratamento de diversas enfermidades humanas. As folhas de Bauhinia monandra são amplamente utilizadas no Brasil como fitoterápico no tratamento do Diabetes Mellitus. A partir das folhas de B. monandra, foi purificada uma lectina galactose-específica, denominada de BmoLL, que também apresentou uma importante capacidade hipoglicemiante. Seguindo as normas propostas pela Portaria nº 116, de 8 de agosto de 1996 do Ministério da Saúde do Brasil, o trabalho objetivou avaliar o potencial de mutagenicidade e toxicidade da BmoLL, mediante a utilização dos testes com cepas de Escherichia coli da linhagem CC104 (Teste de mutagênesedireta), com cepas de Salmonella typhimurium da linhagem TA (Teste de Kado), com plasmídeo pBCKS (Quebra de DNA plasmidial) e com enzima Exonuclease III (Detecção de sítios abásicos). Os resultados demonstraram que a lectina foi incapaz de aumentar a freqüência de mutação reversa das cepas de S. typhimurium, com e sem ativador metabólico.No entanto, uma diminuição significativa na frequência de mutação espontânea foi observada nas cepas de E. coli, especialmente na deficiente de reparo (CC104mutMmutY), sugerindo um potencial antioxidante da lectina. A BmoLL é incapaz de gerar danos genotóxicos e citotóxicos, com base nas concentrações testadas e nos ensaios realizados.
Medicinal plants have been used since ancient times to treat various human diseases.The leaves of Bauhinia monandra are widely used in Brazil as herbal remedies in the treatmentof Diabetes Mellitus. From the leaves of B. monandra, a galactose-specific lectin was purified,called BmoLL, which also showed a significant hypoglycemic capacity. In accordance with therules proposed by Brazil?s Ministry of Health Decree 116 of 08 August 1996, this study aimedat assessing the potential for toxicity and mutagenicity of BmoLL by means of using tests withEscherichia coli strain CC104 (Forward mutagenesis assay) with Salmonella typhimurium strain TA(Kado test), with plasmid pBCKS (Break occurrences in plasmid DNA) and enzyme exonucleaseIII (Search of abasic sites). Results demonstrated that lectin was unable to increase the frequencyof reverse mutation of strains of S. typhimurium, with and without metabolic activity. However,a significant decrease in the frequency of spontaneous mutation was observed in strains ofE. coli, especially in poor repair (CC104mutMmutY), suggesting an anti-oxidant potential of lectin.BmoLL is unable to generate genotoxic and cytotoxic damage, based on the concentrations andtests performed.
Assuntos
Bauhinia , Diabetes Mellitus/tratamento farmacológico , Genotoxicidade , Lectinas/toxicidade , Fitoterapia , Plantas MedicinaisRESUMO
The agglutinin from the red marine alga Hypnea cervicornis (HCA) was tested in models of nociception and inflammation. The role of carbohydrate-binding sites and the systemic toxicity were assessed. HCA (10(-1), 1, and 10 mg/kg) administered i.v. to mice inhibited writhes induced by acetic acid and, at 10 mg/kg, inhibited the second phase of the formalin test, but did not alter the response latency in the hot-plate test. HCA (1 mg/kg) administered i.v. to rats reduced carrageenan-induced paw edema at 1, 2, and 3 h after challenge, but not edema induced by dextran. The neutrophil migration induced by both N-formyl-methionyl-leucyl-phenylalanine (fMLP) and carrageenan was inhibited by HCA at 10(-1), 1, and 10 mg/kg. The combination of HCA (1 mg/kg) and its ligand mucin reversed the lectin inhibitory effect on carrageenan-induced neutrophil migration and acetic acid-induced writhes. The i.v. treatment of rats with HCA (1 mg/kg) for 7 days did not affect body mass; liver, kidney or heart wet weight; blood leukocyte counts; urea, creatinine or serum transaminase activity; or macroscopy of the organs examined. In short, H. cervicornis agglutinin showed important antinociceptive and anti-inflammatory activity via interaction with the lectin carbohydrate-binding site.
Assuntos
Aglutininas/farmacologia , Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Lectinas/farmacologia , Mucinas/metabolismo , Rodófitas/química , Animais , Lectinas/toxicidade , Masculino , Camundongos , Medição da Dor , Ratos , Ratos WistarRESUMO
To meet the demands for food of the expanding world population, there is need of new ways for protecting plant crops against predators and pathogens while avoiding the use of environmentally aggressive chemicals. A milestone in this field was the introduction into crop plants of genes expressing Bacillus thuringiensis entomotoxic proteins. In spite of the success of this new technology, however, there are difficulties for acceptance of these 'anti-natural' products by the consumers and some concerns about its biosafety in mammals. An alternative could be exploring the plant's own defense mechanisms, by manipulating the expression of their endogenous defense proteins, or introducing an insect control gene derived from another plant. This review deals with the biochemical features and mechanisms of actions of plant proteins supposedly involved in defense mechanisms against insects, including lectins, ribosome-inactivating proteins, enzymes inhibitors, arcelins, chitinases, ureases, and modified storage proteins. The potentialities of genetic engineering of plants with increased resistance to insect predation relying on the repertoire of genes found in plants are also discussed. Several different genes encoding plant entomotoxic proteins have been introduced into crop genomes and many of these insect resistant plants are now being tested in field conditions or awaiting commercialization.
Assuntos
Controle de Insetos , Proteínas de Plantas/toxicidade , Plantas Geneticamente Modificadas , Sequência de Aminoácidos , Animais , Inibidores Enzimáticos/toxicidade , Engenharia Genética , Insetos , Lectinas/toxicidade , Dados de Sequência Molecular , Inibidores de Proteases/toxicidade , alfa-Amilases/antagonistas & inibidoresRESUMO
An 87.7% (P < 0.01) and 84% (P < 0.001) of protection against visceral leishmaniasis was achieved in CB hamsters and Balb/c mice, respectively, with saponin combined to the fucose-mannose ligand of Leishmania donovani (FML). However, an undesirable haemolytic effect was described for several saponins. Aiming to improve the formulation with FML/saponin, we comparatively analysed the haemolytic potential of recently characterized plant saponins and currently used adjuvants. The haemolytic activity of steroidic saponins from Agave sisalana; Smilax officinalis as well as commercial saponin (Riedel De Haën's), was higher than that of triterpenoid ones (Bredemeyera floribunda; Periandra mediterranea) and the Freund's complete adjuvant. The concentration resulting in 50% haemolysis was 500 micrograms ml-1 for aluminum hydroxide. The low haemolytic effect of P. mediterranea saponin was abolished by removal of its glycidic moiety and its sapogenin fraction as well as the Freund's Incomplete Adjuvant were non-haemolytic within this range. Furthermore, the adjuvant effect of three doses of P. mediterranea saponin injected with the FML antigen of L. donovani, was assayed in mice, either by the intraperitoneal (i.p.) or the subcutaneous (s.c.) route. The anti-FML IgG antibody levels increased and detectable levels were observed up to 3 months in the s.c. group. The response was expanded in both groups after an injection with a fourth vaccine dose. The IgG response showed increased levels of IgG2a only in the i.p. group, while IgG2b and IgG1 but not IgG3 antibodies were higher than controls in both groups. In conclusion, the results suggest that the recently described triterpenoid fractions of P. mediterranea can be safely used as adjuvant with low or non-haemolytic effect.
Assuntos
Adjuvantes Imunológicos/toxicidade , Antígenos de Protozoários/imunologia , Fucose/imunologia , Proteínas Hemolisinas/toxicidade , Lectinas/imunologia , Leishmania donovani/imunologia , Manose/imunologia , Saponinas/imunologia , Adulto , Animais , Anticorpos Antiprotozoários/biossíntese , Anticorpos Antiprotozoários/efeitos dos fármacos , Anticorpos Antiprotozoários/imunologia , Cricetinae , Fucose/metabolismo , Humanos , Lectinas/toxicidade , Leishmaniose Visceral/imunologia , Leishmaniose Visceral/prevenção & controle , Ligantes , Manose/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Extratos Vegetais/toxicidade , Saponinas/toxicidadeRESUMO
Physicochemical characterization and biological properties of a new toxic protein isolated from soybeans (Glycine max) is reported. The purification procedure consisted basically of ammonium sulfate fractionation, ion exchange, and affinity chromatographies, the latter being used for the removal of the seed's lectin and of its trypsin inhibitor. The highly purified protein, designated soyatoxin, is a single chain acidic protein (pI 4.4-4.6) of 21 kDa, dependent on reduced thiol groups to maintain its solubility and biological activities. The toxin is a metalloprotein containing iron, calcium, zinc, and magnesium. Soyatoxin is highly toxic to mice (LD50 7-8 mg/kg mouse body wt upon intraperitoneal injection). It produces dyspnoea, tonic-clonic convulsions, and flaccid paralysis prior to death of intraperitoneally injected mice. Furthermore, soyatoxin is immunologically related to another toxic protein (canatoxin), isolated from Canavalia ensiformis seeds, which is distinct from soyatoxin in containing 18 x 10 kDa noncovalently bound subunits. Some biological properties including acute intraperitoneal toxicity, canatoxin-like immunoreactivity, hemagglutination, trypsin inhibitory activity, induction of platelet release reaction, and aggregation displayed by soyatoxin were studied and used to differentiate soyatoxin from soybean lectin and trypsin inhibitors.
Assuntos
Glycine max/química , Proteínas de Plantas/isolamento & purificação , Toxinas Biológicas/isolamento & purificação , Animais , Reações Cruzadas , Focalização Isoelétrica , Lectinas/imunologia , Lectinas/isolamento & purificação , Lectinas/toxicidade , Camundongos , Lectinas de Plantas , Proteínas de Plantas/toxicidade , Agregação Plaquetária/efeitos dos fármacos , Coelhos , Toxinas Biológicas/toxicidadeRESUMO
Las lectinas son proteínas capaces de enlazar carbohidratos en forma específica y reversible. Debido a sus interesantes propiedades biológicas se ha mantenido la búsqueda, caracterización y estudio en semillas de la flora venezolana. Estas proteínas presentan estructura oligomérica con propiedades físico-químicas similares y homología estructural (isolectinas); sin embargo, existen lectinas monoméricas. Su función en las plantas no se conoce bien. Presentan actividad mitogénica sobre linfocitos cultivados in vitro y son capaces de aglutinar tanto leucocitos como eritrocitos. Polisacáridos ramificados como la goma arábiga, aumentan el título hemaglutinante de varias lectinas, particularmente aquellas altamente glicosidadas. Algunas lectinas son tóxicas sobre cultivos celulares, así la Concanavalina A causa la disminución de la incorporación de [3H]-timidina en células renales cultivadas in vitro. En Phaseolus vulgaris hay una clara relación entre especificidad hemaglutinante y toxicidad. Animales intubados o alimentados con semillas de las variedades tóxicas de P.vulgaris muestran daño en el borde estriado de las vellosidades del duodeno y yeyuno. La lectina de una varieda tóxica de P.vulgaris interfiere con la utilización de nutrientes a nivel intestinal, inhibe la digestión de carbohidratos de la dieta y la absorción de glucosa en el intestino