RESUMO

Bidens pilosa L. is a medicinal plant popularly used for treatment of liver diseases. In this study, the dry extract of aerial parts of Bidens pilosa and Silymarin, a phytocomplex obtained from the Silybum marianum fruits and marketed as hepatoprotective, were tested in dogs experimentally acutely intoxicated with carbon tetrachloride. The liver activity was evaluated by hematological and biochemical profiles, and histological and ultrasound analyzes. It was observed that the lowest serum activities of ALT and serum concentrations of total bilirubin occurred in the groups treated with the dry extract of Bidens pilosa, while only decreased serum concentrations of total bilirubin occurred in the group treated with Silymarin. Best liver recovery was also observed for the dry extract of B. pilosa at a 400mg/Kg dose by ultrasonography. This study showed that the dry extract of Bidens pilosa acted more efficiently in the treatment of acute toxic hepatitis induced in dogs than Silymarin.(AU)

Bidens pilosa L. é uma planta medicinal utilizada popularmente para tratamento de doenças hepáticas. Neste trabalho, o extrato seco das partes aéreas da Bidens pilosa e a silimarina, um fitocomplexo obtido dos frutos da Silybum marianum e comercializado como hepatoprotetor, foram testados em cães intoxicados experimentalmente de forma aguda com tetracloreto de carbono. A atividade hepática foi avaliada por meio dos perfis hematológico e bioquímico, análises histológica e ultrassonográfica. Observou-se que, nos grupos tratados com o extrato seco da Bidens pilosa, ocorreram as menores atividades séricas da ALT e de concentrações séricas de bilirrubina total, enquanto no grupo tratado com silimarina, ocorreu apenas diminuição de concentrações séricas de bilirrubina total. Melhor recuperação hepática também foi verificada para o extrato seco de B. pilosa na dose de 400mg/kg por ultrassonografia. Este estudo evidenciou que o extrato seco da Bidens pilosa atuou de forma mais eficiente no tratamento da hepatite aguda tóxica induzida em cães do que a silimarina.(AU)

Assuntos

RESUMO

Bidens pilosa L. is a medicinal plant popularly used for treatment of liver diseases. In this study, the dry extract of aerial parts of Bidens pilosa and Silymarin, a phytocomplex obtained from the Silybum marianum fruits and marketed as hepatoprotective, were tested in dogs experimentally acutely intoxicated with carbon tetrachloride. The liver activity was evaluated by hematological and biochemical profiles, and histological and ultrasound analyzes. It was observed that the lowest serum activities of ALT and serum concentrations of total bilirubin occurred in the groups treated with the dry extract of Bidens pilosa, while only decreased serum concentrations of total bilirubin occurred in the group treated with Silymarin. Best liver recovery was also observed for the dry extract of B. pilosa at a 400mg/Kg dose by ultrasonography. This study showed that the dry extract of Bidens pilosa acted more efficiently in the treatment of acute toxic hepatitis induced in dogs than Silymarin.(AU)

Bidens pilosa L. é uma planta medicinal utilizada popularmente para tratamento de doenças hepáticas. Neste trabalho, o extrato seco das partes aéreas da Bidens pilosa e a silimarina, um fitocomplexo obtido dos frutos da Silybum marianum e comercializado como hepatoprotetor, foram testados em cães intoxicados experimentalmente de forma aguda com tetracloreto de carbono. A atividade hepática foi avaliada por meio dos perfis hematológico e bioquímico, análises histológica e ultrassonográfica. Observou-se que, nos grupos tratados com o extrato seco da Bidens pilosa, ocorreram as menores atividades séricas da ALT e de concentrações séricas de bilirrubina total, enquanto no grupo tratado com silimarina, ocorreu apenas diminuição de concentrações séricas de bilirrubina total. Melhor recuperação hepática também foi verificada para o extrato seco de B. pilosa na dose de 400mg/kg por ultrassonografia. Este estudo evidenciou que o extrato seco da Bidens pilosa atuou de forma mais eficiente no tratamento da hepatite aguda tóxica induzida em cães do que a silimarina.(AU)

Assuntos

RESUMO

Due to its chemical composition and use in folk medicine, the dry standardized extract of Apeiba tibourbou Aubl. (Tiliaceae) leaves (DSEAT) was tested to assess its hepatoprotective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. The animals were treated with DSEAT previously for 7 days, at doses of 25, 50, 100, 200, and 400 mg/kg and 18 mg/kg of rosmarinic acid; the liver damage was induced by administering CCl4 intraperitoneally (i.p.) at days 3 and 7, and 1 h before treating with DSEAT. The hepatoprotective activity was assessed using various biochemical assays such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gammaglutamyltransferase (GGT), malondialdehyde (MDA) and histopatological studies. DSEAT doses of 400, 200, and 100 mg/kg were not capable of protecting the liver against CCl4. However, the dose of 50 mg/kg reduced AST by 31.50% the dose of 25 mg/kg reduced GGT by 57.18% compared to the CCl4 (p < 0.05). In the liver, DSEAT dose of 50 mg/kg and rosmarinic acid reduced MDA by 27.45% and 63.61%, respectively, whereas in plasma, MDA was reduced in all the groups treated with DSEAT and rosmarinic acid. In conclusion, DSEAT exhibits hepatoprotective effect only at low doses and antioxidant activity in vivo after peroral administration. The experimental protocol was approved by the Animal Research Ethics Committee of UFG (CEUA, no. 177/2011).

Assuntos

RESUMO

Due to its chemical composition and use in folk medicine, the dry standardized extract of Apeiba tibourbou Aubl. (Tiliaceae) leaves (DSEAT) was tested to assess its hepatoprotective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. The animals were treated with DSEAT previously for 7 days, at doses of 25, 50, 100, 200, and 400 mg/kg and 18 mg/kg of rosmarinic acid; the liver damage was induced by administering CCl4 intraperitoneally (i.p.) at days 3 and 7, and 1 h before treating with DSEAT. The hepatoprotective activity was assessed using various biochemical assays such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gammaglutamyltransferase (GGT), malondialdehyde (MDA) and histopatological studies. DSEAT doses of 400, 200, and 100 mg/kg were not capable of protecting the liver against CCl4. However, the dose of 50 mg/kg reduced AST by 31.50% the dose of 25 mg/kg reduced GGT by 57.18% compared to the CCl4 (p < 0.05). In the liver, DSEAT dose of 50 mg/kg and rosmarinic acid reduced MDA by 27.45% and 63.61%, respectively, whereas in plasma, MDA was reduced in all the groups treated with DSEAT and rosmarinic acid. In conclusion, DSEAT exhibits hepatoprotective effect only at low doses and antioxidant activity in vivo after peroral administration. The experimental protocol was approved by the Animal Research Ethics Committee of UFG (CEUA, no. 177/2011).(AU)

Assuntos

RESUMO

Fifteen yearling steers were used to study the preventive effect of vitamin E on the protection against free radicals produced by carbon tetrachloride (CCl4). The animals were randomly divided in three equal groups and treated as follows: group A--previously injected (i.m.) with 15 IU/kg BW on the 15th and second day before the trial and drenched with 0.05 ml/kg BW CCl4; group B--only drenched with the same dose of CCl4; group C--drenched with a placebo. Food intake was recorded and blood samples collected daily for 8 days after the CCl4 drenching to compare the activity of aspartate aminotransferase (AST), gamma-glutamyltransferase (gamma-GT) and the levels of erythrocyte reduced glutathione (GSH) and serum malonyldialdehyde (MDA). Food intake was reduced in group B for the first 3 days (P < 0.05); higher activities of AST and gamma-GT were observed in the poisoned groups, nevertheless the overall values were lower in the group A than B (P < 0.02); only the group A reached the basal values of AST at the seventh day; higher levels of GSH and MDA were recorded in the poisoned cattle indicating the generation of free radicals. It was concluded that the preventive use of Vitamin E lessened the damage in hepatic tissue caused by the free radicals and prevented the anorexia caused by CCl4.

Assuntos