RESUMO
Ozone therapy acts in the body inducing controlled oxidative stress, thereby improving the antioxidant, immune and circulatory responses. However, very little is known about how this therapy affects oxidative stress indicators in dogs. We aimed to assess the clinical, hematological, biochemical and oxidative stress parameters of healthy dogs subjected to ozone therapy and oxygen therapy by rectal insufflation. Ten healthy dogs were allocated into three experimental groups in a cross-over design: control, without intervention; ozone, which received 100 µg of O3/kg through rectal insufflation; and oxygen, which received an ozone-equivalent volume of medicinal O2 through rectal insufflation. Dogs received four applications weekly and were followed up until the seventh week. Ozone therapy significantly increased the weight, mean corpuscular volume and mean platelet volume and decreased total cholesterol of treated dogs. Regarding oxidative stress, ozone therapy reduced total antioxidant capacity by ferric reduction (TAC-FRAP) in D7 compared with baseline and the control, significantly increased total antioxidant capacity by cupric reduction (TAC-CUPRAC) in D42 and D49 compared with the control group, caused an increase in uric acid compared with the oxygen group and decreased lipid peroxidation on D21 compared with the control group. In conclusion, ozone therapy through rectal insufflation causes transient oxidative stress followed by an antioxidant response and discreetly interferes with a few clinical, hematological and biochemical variables in healthy dogs, although variables still remained within the reference ranges for the species, thus proving the safety of the therapy. Furthermore, oxygen therapy causes oxidative stress without inducing a subsequent antioxidant response.
Assuntos
Estudos Cross-Over , Insuflação , Estresse Oxidativo , Ozônio , Reto , Animais , Cães , Ozônio/administração & dosagem , Estresse Oxidativo/efeitos dos fármacos , Reto/efeitos dos fármacos , Masculino , Insuflação/veterinária , Feminino , AntioxidantesRESUMO
Ozone (O3 ) therapy has been used to improve peripheral tissue oxygenation in humans and domestic animals. The goal of the present study was to characterize histological changes in the endometria of healthy equines following tissue exposure to gas mixtures enriched with different concentrations of O3 . Cycling mares without endometrial degeneration were divided into three groups according to treatment (n = 9 mares/group). The uteri from the O3 , ½O3 and control groups were insufflated for 3 min with gas containing 42, 21 and 0 µg O3 ml-1 , respectively. Treatments were performed every three days from D0 to D6. Endometrial samples were collected immediately before the first treatment and 24 hr after the last treatment. The following nine histological parameters were evaluated: (i) the number of endometrial blood vessels, (ii) endometrial vascular degree (EVD), (iii) increase rate of blood vessels, (iv) increase rate of EVD, (v) glandular total area, (vi) glandular lumen area, (vii) intraglandular secretion area, (viii) glandular epithelial height and (ix) luminal epithelial height. In the O3 group, a positive effect from treatment (p < .01) was detected for all vascular parameters (i, ii, iii and iv), glandular total area, intraglandular secretion area and glandular epithelial height. Compared to the control group, the ½O3 group had greater (p < .01) EVD (84.1 ± 12%) and a higher increase rate of blood vessels (151.9 ± 47.1%). Uterine insufflation with low or intermediate concentrations of the O2 -O3 gas mixture induced endometrial angiogenesis. Morphometry, but not morphology, of the endometrial glands was affected by local O3 therapy. These findings would be of great significance for the development of new therapies for infertility in mares.
Assuntos
Endométrio/efeitos dos fármacos , Neovascularização Fisiológica/efeitos dos fármacos , Ozônio/farmacologia , Animais , Endométrio/irrigação sanguínea , Feminino , Cavalos , Insuflação/veterinária , Oxigênio/farmacologia , Útero/cirurgiaRESUMO
Background: Mastitis is an economically important disease leading cost associated problems. The most accurate relationship between intramammary infection and somatic cell count (SCC) can be evaluated at quarter level when SCC exceeds 100.000 cells/mL. In subclinical mastitis, pathogens do not cause enough disruption in the alveolar tissue to be seen in the milk but SCC increase indicates the infected milk. Ozone (O3 ) is an unstable polymerized oxygen created by the passage of air or oxygen over high energy electrodes within an ozone generator system or by ultraviolet light. The aim of this study was to compare the treatment efficacy of ozone (O3 ) insufflation daily via latex free syringe in different doses (30-60-70 µg/mL) and high dose O3 insufflation (70 µg/mL) with combination of antibiotic and only antibiotic treatment in dairy cows with clinical mastitis.Materials, Methods & Results: A total of 32 lactating cows with clinical signs of mastitis whom had got 79 infected mammary quarters were used for the study. Mastitis was diagnosed by typical clinical symptoms of the mammary gland and general clinical symptoms of systemic infection. California mastitis test (CMT) was performed to all quarters and recorded before the treatment. Somatic cell count (SCC) of each milk sample of the infected quarters was recorded before the treatment. Microbiological examination was conducted on aseptically collected milk samples from infected quarters before the treatment. Ozone insufflation was applied daily in different doses (30, 60 and 70 µg/mL) intramammary via latex free 100 mL syringe every day for one week and only antibiotic was administered intramuscularly to the mastitic cows for 5 days and high dose ozone insufflation (70 µg/mL) with combination of antibiotic therapy after milking of the animals. On day 8; CMT and SCC were repeated to evaluate the efficacy of the treatments on the milk samples.[...](AU)
Assuntos
Animais , Feminino , Bovinos , Ozônio/administração & dosagem , Mastite Bovina/tratamento farmacológico , Ozônio/uso terapêutico , Antibacterianos/uso terapêutico , Insuflação/veterináriaRESUMO
Background: Mastitis is an economically important disease leading cost associated problems. The most accurate relationship between intramammary infection and somatic cell count (SCC) can be evaluated at quarter level when SCC exceeds 100.000 cells/mL. In subclinical mastitis, pathogens do not cause enough disruption in the alveolar tissue to be seen in the milk but SCC increase indicates the infected milk. Ozone (O3 ) is an unstable polymerized oxygen created by the passage of air or oxygen over high energy electrodes within an ozone generator system or by ultraviolet light. The aim of this study was to compare the treatment efficacy of ozone (O3 ) insufflation daily via latex free syringe in different doses (30-60-70 µg/mL) and high dose O3 insufflation (70 µg/mL) with combination of antibiotic and only antibiotic treatment in dairy cows with clinical mastitis.Materials, Methods & Results: A total of 32 lactating cows with clinical signs of mastitis whom had got 79 infected mammary quarters were used for the study. Mastitis was diagnosed by typical clinical symptoms of the mammary gland and general clinical symptoms of systemic infection. California mastitis test (CMT) was performed to all quarters and recorded before the treatment. Somatic cell count (SCC) of each milk sample of the infected quarters was recorded before the treatment. Microbiological examination was conducted on aseptically collected milk samples from infected quarters before the treatment. Ozone insufflation was applied daily in different doses (30, 60 and 70 µg/mL) intramammary via latex free 100 mL syringe every day for one week and only antibiotic was administered intramuscularly to the mastitic cows for 5 days and high dose ozone insufflation (70 µg/mL) with combination of antibiotic therapy after milking of the animals. On day 8; CMT and SCC were repeated to evaluate the efficacy of the treatments on the milk samples.[...]
Assuntos
Feminino , Animais , Bovinos , Antibacterianos/uso terapêutico , Mastite Bovina/tratamento farmacológico , Ozônio/administração & dosagem , Ozônio/uso terapêutico , Insuflação/veterináriaRESUMO
PURPOSE: To assess comparatively the inflammatory response that follows CO(2) or Ringer's lactate joint capsular distension of horses submitted to experimental arthroscopy METHODS: Each animal was submitted to a bilateral tarsocrural arthroscopy employing gas distention in one joint and fluid distention in the contralateral joint. Synovial fluid was evaluated at 0, six, 12, 24 and 48 hours post-operative. RESULTS: The use of CO(2) for arthroscopy causes an acute and mild synovitis alike to the liquid capsular distension, showing similar synovial fluid increase of leukocytes, TP, and TNF-α. Although synovial fluid PGE(2) content was higher in joints submitted to CO(2) distension, lower levels of hemoglobin and leukocytes oxidative burst after surgery indicates that CO(2) arthroscopy decreased intra-articular bleeding and activation of infiltrating leukocytes. CONCLUSIONS: The use of CO(2) for arthroscopic examination causes acute and mild synovitis that is similar to the effects caused by the liquid capsular distension. CO2 also seems to decrease intra-articular bleeding and activation of leukocytes.
Assuntos
Artroscopia/veterinária , Insuflação/veterinária , Articulações/cirurgia , Animais , Artroscopia/métodos , Dióxido de Carbono/administração & dosagem , Feminino , Citometria de Fluxo , Cavalos , Injeções Intra-Articulares/veterinária , Insuflação/efeitos adversos , Insuflação/métodos , Masculino , Líquido Sinovial , Sinovite/etiologiaRESUMO
PURPOSE: To assess comparatively the inflammatory response that follows CO2 or Ringer's lactate joint capsular distension of horses submitted to experimental arthroscopy METHODS: Each animal was submitted to a bilateral tarsocrural arthroscopy employing gas distention in one joint and fluid distention in the contralateral joint. Synovial fluid was evaluated at 0, six, 12, 24 and 48 hours post-operative. RESULTS: The use of CO2 for arthroscopy causes an acute and mild synovitis alike to the liquid capsular distension, showing similar synovial fluid increase of leukocytes, TP, and TNF-α. Although synovial fluid PGE2 content was higher in joints submitted to CO2 distension, lower levels of hemoglobin and leukocytes oxidative burst after surgery indicates that CO2 arthroscopy decreased intra-articular bleeding and activation of infiltrating leukocytes. CONCLUSIONS: The use of CO2 for arthroscopic examination causes acute and mild synovitis that is similar to the effects caused by the liquid capsular distension. CO2 also seems to decrease intra-articular bleeding and activation of leukocytes.
OBJETIVO: Avaliar comparativamente a resposta inflamatória decorrente da distensão líquida ou gasosa em cavalos submetidos ao exame artroscópico. MÉTODOS: Cada animal foi submetido a uma artroscopia bilateral tarsocrural empregando uma distensão com gás em uma articulação e líquido na articulação contralateral. O líquido sinovial foi avaliado as zero, seis, 12, 24 e 48 horas do pós-operatório. RESULTADOS: A utilização de CO2 para a artroscopia provoca uma sinovite aguda e leve tal como a distensão capsular por líquido, mostrando um aumento similar de leucócitos, TP (proteína total) e TNF-a. Embora no líquido sinovial a quantidade de PGE2 tenha sido maior nas articulações submetidas à distensão por CO2, níveis mais baixos de hemoglobina e explosão oxidativa de leucócitos após a cirurgia indica que a artroscopia com CO2 diminuiu o sangramento intra-articular e ativação de leucócitos. CONCLUSÕES: A utilização de CO2 para exame artroscópico provoca uma sinovite aguda e leve que são semelhantes aos efeitos causados pela distensão capsular por líquido. O CO2 também parece diminuir o sangramento intra-articular e a ativação de leucócitos.
Assuntos
Animais , Feminino , Masculino , Artroscopia/veterinária , Insuflação/veterinária , Articulações/cirurgia , Artroscopia/métodos , Dióxido de Carbono/administração & dosagem , Citometria de Fluxo , Cavalos , Injeções Intra-Articulares/veterinária , Insuflação/efeitos adversos , Insuflação/métodos , Líquido Sinovial , Sinovite/etiologiaRESUMO
La cirugía laparoscópica ha llegado a ser clínicamente relevante. Siempre que se lleva a cabo este tipo de cirugías se tiene que insuflar la cavidad abdominal con dióxido de carbono (CO2). Nosotros evaluamos el uso de CO2 y helio (He) en la producción de neumoperitoneo experimental. Doce perros mestizos fueron sometidos a neumoperitoneo bajo anestesia con halotano al 1 por ciento. Todos los animales se ventilaron mecánicamente con volumen 15 mL/kg, frecuencia respiratoria de 20/min y 100 por ciento de fracción inspirada de O2. Los animales fueron divididos en 2 grupos de estudio. Grupo I: (n = 6) se les produjo neumoperitoneo experimental con CO2 y grupo II: (n = 6) neumoperitoneo experimental con He. El neumoperitoneo se estableció a través de un trocar de 5 mm que se insertó a nivel de la cicatriz umbilical. La presión de insuflación de la cavidad fue de 10 cmH2O durante 30 minutos, 15 cmH2O por 30 minutos y 20 cmH2O también durante 30 minutos. Los parámetros hemodinámicos, gases sanguíneos y la presión inspiratoria máxima fueron evaluados después de la insuflación cada 30 minutos de establecido el neumoperitoneo y 30 minutos después de desinsuflar el abdomen. En los resultados se observó que el gasto cardiaco disminuyó en ambos grupos durante la producción del neumoperitoneo. La presión venosa central (PVC) y la resistencia vascular pulmonar se mantuvo similar en ambos grupos. Las gasometrías del grupo I mostraron incrementó en la pCO2 venosa a los 30 minutos de haberse iniciado el neumoperitoneo, a los 30 mintos de mantener la presión a 20 cmH2O y al finalizar el estudio, mientras que el grupo II no sufrió cambios. La presión inspiratoria máxima (PIM) se incrementó en ambos grupos. Concluimos que no hay diferencia entre la producción del neumoperitoneo con CO2 o He.